Abstract:
:Chlorambucil-induced apoptosis was assessed by three different flow cytometric methods in B-cell chronic lymphocytic leukaemia (B-CLL) cells cultured in vitro and the results were compared with those derived from the morphological assessment of the same samples. Spontaneous apoptosis was consistently observed in the control cultures in the absence of drug but this accounted for less than 12% of all cells in every case. The methods under investigation were the Annexin V labelling assay, the terminal deoxynucleotidyl transferase (TdT) end-labelling assay and the labelling of a 38 kDa mitochondrial membrane protein (7A6 antigen) which is exposed on cells undergoing apoptotic cell death (Apo2.7 assay). The Annexin V assay consistently stained a higher percentage of cells and with a greater separation between the positive and negative cell populations. We conclude that the phosphatidyl serine translocation to the outer leaflet of the cell membrane following an apoptotic signal, as labelled by Annexin V, probably occurs before the development of the DNA strand breaks or the exposure of 7A6 antigen in those cells triggered to die by apoptosis.
journal_name
Leuk Resjournal_title
Leukemia researchauthors
Pepper C,Thomas A,Tucker H,Hoy T,Bentley Pdoi
10.1016/s0145-2126(98)00013-7subject
Has Abstractpub_date
1998-05-01 00:00:00pages
439-44issue
5eissn
0145-2126issn
1873-5835pii
S0145-2126(98)00013-7journal_volume
22pub_type
杂志文章abstract::As a part of our continuing efforts to develop gene therapy for acute myelogenous leukemia (AML), this study was undertaken to evaluate the possibility of using autologous bone marrow stromal fibroblasts (BMSFs) as a target cell population. Autologous BMSFs in AML were isolated from the stromal layers of long-term bon...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(01)00134-5
更新日期:2002-04-01 00:00:00
abstract::Hemopoietic responses were studied in splenectomized mice after transplantation of the hemopoietic colony stimulating activity producing leukemia, L8313. In non-splenectomized mice, the number of peripheral leukocytes was elevated to 4 x 10(5)/mm3 after transplantation. In contrast, it was below 1.7 x 10(4)/mm3 in spl...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(89)90159-8
更新日期:1989-01-01 00:00:00
abstract::Two AML patients, whose leukemic clonogenic cells totally reacted to the anti-lactofucopentaose III S4-7 monoclonal antibody (MoAb), underwent autologous bone marrow transplantation, in first complete remission, after in-vitro purging with S4-7 MoAb and complement. After ablative chemotherapy (BAVC regimen) and reinfu...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(87)90050-6
更新日期:1987-01-01 00:00:00
abstract::The inaugural meeting of the International Working Group on MDS cytogenetics convened 22-23 October 2007 in Chicago, IL. Under the sponsorship of the Myelodysplastic Syndromes Foundation, the group was organized to address the substantial need for worldwide standardized cytogenetic testing for MDS in clinical practice...
journal_title:Leukemia research
pub_type: 总体
doi:10.1016/j.leukres.2008.03.009
更新日期:2008-09-01 00:00:00
abstract::We evaluated the molecular mechanism of telomerase activation by erythropoietin (EPO) in human erythroleukemic JAS-REN-A cells. Telomerase activity increased 3-4 fold after 3-24h of culture with EPO and was associated with increases in c-myc mRNA after 1-3h, of c-Myc protein after 3-6h, and of human telomerase reverse...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2010.11.002
更新日期:2011-03-01 00:00:00
abstract::We have previously shown that HL-60 cells exposed to all-trans retinoic acid (ATRA) after treatment with a non-cytotoxic concentration of vincristine (VCR) result in granulocytic maturation and differentiation, suggesting that VCR might exhibit a synergistic action with ATRA in the treatment of acute promyelocytic leu...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(96)00090-2
更新日期:1997-01-01 00:00:00
abstract::MDS is primarily a disease of the elderly. Cases who give a history of exposure to X-rays, cytotoxic drugs or leukaemogenic chemicals may be younger. Many cases of MDS present because of an incidental blood count. The most prominent clinical features are those of anaemia, neutropenia, thrombocytopenia. Because haemopo...
journal_title:Leukemia research
pub_type: 杂志文章,评审
doi:10.1016/0145-2126(92)90106-h
更新日期:1992-01-01 00:00:00
abstract::Recombinant TNF was capable of inducing differentiation of human leukemic ML-1 cells in the monocytic pathway. Recombinant interleukin 6 did not have the activity but it could significantly increase the activity of recombinant TNF. Both of these molecules were found to play a similar role in PWM-induced conditioned me...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(90)90010-7
更新日期:1990-01-01 00:00:00
abstract::The glycosylation of beta-hexosaminidase was investigated in the transformed cell-line, CCRF/CEM, derived from a human acute lymphoblastic leukaemia, and comparisons were made with enzyme from normal human skin fibroblasts. A series of studies including neuraminidase sensitivity, lectin chromatography, Biogel P4 chrom...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(88)90146-4
更新日期:1988-01-01 00:00:00
abstract::Flow cytometry is commonly used to establish the diagnosis of chronic lymphocytic leukemia (CLL). A defined combination of antibodies discriminates between normal B cells and CLL cells (coexpression of CD5, CD19, and CD23). The receptor tyrosine-like orphan receptor one (ROR1) is an embryonic glycoprotein involved in ...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2011.04.006
更新日期:2011-10-01 00:00:00
abstract::With the application of adequately sensitive tests, it is now becoming evident that more than 90% of patients with conventionally-defined polycythemia vera (PV) carry the somatic JAK2V617F mutation in their granulocytes. However, the specific mutation is also found in other classic and atypical myeloproliferative diso...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2006.01.004
更新日期:2006-06-01 00:00:00
abstract::To study the role of SDF-1/CXCR4 axis in MDS, the expression of SDF-1 and CXCR4, VEGF, MVD and apoptosis were measured in MDS. The results showed that the expression of SDF-1 of the low-grade MDS is higher than that of the high-grade MDS and the control. The high-grade MDS had a significantly higher CXCR4 expression o...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2011.06.017
更新日期:2012-03-01 00:00:00
abstract::Treatment failure of patients with acute myelogenous leukemia (AML) is frequently due to the development of multidrug resistance phenotype blasts. We have expressed a fusion protein consisting of human granulocyte-macrophage colony stimulating factor (GMCSF) fused to the N-terminus of a lectin-deficient ricin toxin B ...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(97)00043-x
更新日期:1997-07-01 00:00:00
abstract::In a prospective study of 27 patients with acute myeloid leukaemia (AML) quality of life was studied using three different instruments, Life Ingredient Profile, Karnofsky Performance Scale and Vitagram. The course of the disease was followed during the induction period, remission, relapse and in the terminal phase. We...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(94)90028-0
更新日期:1994-04-01 00:00:00
abstract::The colony-forming capacity of the peripheral blood stem/progenitor cells (PBSC) in different forms of myelodysplastic syndrome (MDS) was investigated. In most cases of refractory anemia (RA) the colony growth of PBSC was definitely reduced as compared to the controls. However, in RA with unfavorable chromosomal aberr...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(01)00064-9
更新日期:2001-11-01 00:00:00
abstract::Dietary bioflavonoids are secondary metabolites of plants that are known to have a variety of bio-effects, including anti-cancer activity. In this study, we examined the effects of flavonoids on the growth of human leukemia cells and found that certain flavonoids induce apoptosis in a variety of human leukemia cells. ...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2004.11.015
更新日期:2005-05-01 00:00:00
abstract::We studied 98 female patients in remission (2-240 months) from childhood ALL to determine the clonality status of their hematopoiesis. Thirty-one (31.6%) were heterozygous at the PGK locus for the BstX1 endonuclease restriction site, permitting X-linked clonality assays to be performed. Two patients were in relapse at...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(94)90121-x
更新日期:1994-02-01 00:00:00
abstract::Acute myeloid leukemia is an aggressive disease that arises from clonal expansion of malignant hematopoietic precursor cells of the bone marrow. Deletions on the long arm of chromosome 9 (del(9q)) are observed in 2% of acute myeloid leukemia patients. Our deletion analysis in a cohort of 31 del(9q) acute myeloid leuke...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2018.11.007
更新日期:2019-01-01 00:00:00
abstract::We established a plasmacytoid dendritic cell (pDC) line (PMDC05) from leukemia cells of pDC leukemia. PMDC05 cells were positive for CD4, CD56, CD33, HLA-DR, CD123 (IL-3Ralpha) and CD86 in the absence of lineage markers. mRNA of TLR1, TLR2, TLR4, TLR7 and TLR9 was clearly expressed and among these TLRs, TLR7 was promi...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2009.03.047
更新日期:2009-09-01 00:00:00
abstract::Epstein-Barr virus (EBV) can induce a broad spectrum of hematological diseases, especially in immune deficient patients. We assayed for receptor for EBV (EBVR) using fluoresceinated viral particles on 44 human hematopoietic cell lines derived from patients with T, B, and non-T, non-B acute lymphocytic leukemia (ALL), ...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(85)90085-2
更新日期:1985-01-01 00:00:00
abstract::Argininosuccinate synthetase (ASS) is a ATP-dependent and rate-limiting enzyme of the urea cycle which catalyzes L-citrulline to L-arginine in combination with argininosuccinate lyase (ASL). We demonstrate here that (a) human normal T and B lymphocytes did not express ASS activity, (b) however, three adult T leukemia ...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(90)90184-b
更新日期:1990-01-01 00:00:00
abstract::It has become routine practice to culture bone marrow and/or unstimulated peripheral blood for cytogenetic analysis due to associations of consistent chromosomal abnormalities within specific subgroups of leukemias. Unfortunately, two leukemias for which numerous chromosomal aberrations have been recorded, acute myelo...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(89)90085-4
更新日期:1989-01-01 00:00:00
abstract::Fifty-three patients with Ph positive chronic myeloid leukaemia in blastic phase were studied. Additional abnormalities were found in 29 (55%) patients and were more common in myeloid (64%) than lymphoid (45%) blast crisis. The most frequent were +Ph (32%), +8 (28%), +19 (19%), +20 (9%) and +21 (9%). i(17q) (9%) was a...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(94)90023-x
更新日期:1994-05-01 00:00:00
abstract::All-trans-retinoic acid (ATRA) has been proven active against a range of malignancies in isolated tissue culture systems and in human clinical trials, but the duration of its effects has been transient. Recent evidence indicates that the basis for the limited duration of ATRA's activity, at least in one form of leukem...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(94)90040-x
更新日期:1994-08-01 00:00:00
abstract::The usefulness of a new method to detect B-cell monoclonality named VR, which is a combination of the digestion with restriction enzymes and vectorette PCR, in aspiration biopsy-nucleic acid diagnosis (ABND) of thyroid malignant lymphoma was investigated. ABND was performed in 12 patients in whom malignant lymphoma wa...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2007.03.019
更新日期:2008-01-01 00:00:00
abstract::The goal of this study was to ascertain the specific effects of 17-N-Allylamino-17-demethoxygeldanamycin (17-AAG) treatment in human acute myelogenous leukemia (AML). Four human leukemia cell lines were treated with varying doses of 17-AAG followed by analysis of toxicity, apoptosis, proliferation, and cell cycle. Cel...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2010.05.024
更新日期:2010-11-01 00:00:00
abstract::Stem cell factor (SCF) is a growth factor that promotes the survival, proliferation, and differentiation of hematopoietic cells. SCF and its receptor, Kit, are normally present in both cell surface and soluble forms. Both forms of Kit can bind SCF. However, the function of soluble Kit is unknown. In order to determine...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(00)00122-3
更新日期:2001-05-01 00:00:00
abstract:BACKGROUND:Chronic Myeloid Leukemia (CML) is characterized by the overproduction of BCR-ABL, a tyrosine kinase with constitutive activity, in which the majority of CML patients have e13a2 or e14a2 transcripts. Reckoned the possible associations between the hematologic and molecular features of the disease, a profound u...
journal_title:Leukemia research
pub_type: 杂志文章,评审
doi:10.1016/j.leukres.2021.106512
更新日期:2021-01-19 00:00:00
abstract::Mononuclear cells from a bone marrow infiltrated by plasmacytoma cells were examined by electron microscopy. An analysis was performed according to different cytoarchitectural forms of the endoplasmic reticulum (ER). Six types of plasma cells could be distinguished. The bone marrow cells were treated with an anti-idio...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(84)90090-0
更新日期:1984-01-01 00:00:00
abstract:BACKGROUND:Therapy strategies for myelodysplastic syndromes (MDS) and juvenile myelomonocytic leukemia (JMML) vary considerably. OBJECTIVE:To review the treatment of Brazilian children who were diagnosed with MDS or JMML in the past decade and reported to the Brazilian Cooperative Group on Pediatric Myelodysplastic Sy...
journal_title:Leukemia research
pub_type: 杂志文章,多中心研究
doi:10.1016/j.leukres.2004.01.008
更新日期:2004-09-01 00:00:00