Polar zipper sequence in the high-affinity hemoglobin of Ascaris suum: amino acid sequence and structural interpretation.

abstract：:By using a combination of genetic, pharmacological, and anatomical approaches, we show that the melanocortin 4 receptor (MC4R), implicated in the control of food intake and energy expenditure, also modulates erectile function and sexual behavior. Evidence supporting this notion is based on several findings: (i) a high...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Van der Ploeg LH,Martin WJ,Howard AD,Nargund RP,Austin CP,Guan X,Drisko J,Cashen D,Sebhat I,Patchett AA,Figueroa DJ,DiLella AG,Connolly BM,Weinberg DH,Tan CP,Palyha OC,Pong SS,MacNeil T,Rosenblum C,Vongs A,Tang R

更新日期：2002-08-20 00:00:00

abstract：:The putative oxidation of hydroethidine (HE) has become a widely used fluorescent assay for the detection of superoxide in cultured cells. By covalently joining HE to a hexyl triphenylphosphonium cation (Mito-HE), the HE moiety can be targeted to mitochondria. However, the specificity of HE and Mito-HE for superoxide ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Robinson KM,Janes MS,Pehar M,Monette JS,Ross MF,Hagen TM,Murphy MP,Beckman JS

更新日期：2006-10-10 00:00:00

abstract：:The Arabidopsis DEMETER (DME) DNA glycosylase demethylates the maternal genome in the central cell prior to fertilization and is essential for seed viability. DME preferentially targets small transposons that flank coding genes, influencing their expression and initiating plant gene imprinting. DME also targets interg...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang C,Hung YH,Rim HJ,Zhang D,Frost JM,Shin H,Jang H,Liu F,Xiao W,Iyer LM,Aravind L,Zhang XQ,Fischer RL,Huh JH,Hsieh TF

更新日期：2019-08-27 00:00:00

abstract：:Clostridium botulinum neurotoxins (BoNTs), the most potent toxins known, disrupt neurotransmission through proteolysis of proteins involved in neuroexocytosis. The light chains of BoNTs are unique zinc proteases that have stringent substrate specificity and require exceptionally long substrates. We have determined the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Segelke B,Knapp M,Kadkhodayan S,Balhorn R,Rupp B

更新日期：2004-05-04 00:00:00

abstract：:Thermotaxis by individual amoebae of Dictyostelium discoideum on a temperature gradient is described. These amoebae show positive thermotaxis at temperatures between 14 degrees C and 28 degrees C shortly (3 hr) after food depletion. Increasing time on the gradient reduces the positive thermotactic response at the lowe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hong CB,Fontana DR,Poff KL

更新日期：1983-09-01 00:00:00

abstract：:In a previous study we demonstrated that vesicular stomatitis virus (VSV) can be used as a vector to express a soluble protein in mammalian cells. Here we have generated VSV recombinants that express four different membrane proteins: the cellular CD4 protein, a CD4-G hybrid protein containing the ectodomain of CD4 and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schnell MJ,Buonocore L,Kretzschmar E,Johnson E,Rose JK

更新日期：1996-10-15 00:00:00

abstract：:Many synthetic or natural fibers are produced via the transformation of a liquid solution into a solid filament, which allows the wet processing of high molecular weight polymers, proteins, or inorganic particles. Synthetic wet-spun fibers are used in our everyday life from clothing to composite reinforcement applicat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mercader C,Lucas A,Derré A,Zakri C,Moisan S,Maugey M,Poulin P

更新日期：2010-10-26 00:00:00

abstract：:A comprehensive nonredundant database of 475 cocrystallized protein-protein complexes was used to study low-resolution recognition, which was reported in earlier docking experiments with a small number of proteins. The docking program GRAMM was used to delete the atom-size structural details and systematically dock th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vakser IA,Matar OG,Lam CF

更新日期：1999-07-20 00:00:00

abstract：:All cell functions that involve membrane deformation or a change in cell shape (e.g., endocytosis, exocytosis, cell motility, and cytokinesis) are regulated by membrane tension. While molecular contacts between the plasma membrane and the underlying actin cytoskeleton are known to make significant contributions to mem...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nambiar R,McConnell RE,Tyska MJ

更新日期：2009-07-21 00:00:00

abstract：:By employing the nuclear DNA of the African rice Oryza glaberrima as a reference genome, the timing, natures, mechanisms, and specificities of recent sequence evolution in the indica and japonica subspecies of Oryza sativa were identified. The data indicate that the genome sizes of both indica and japonica have increa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ma J,Bennetzen JL

更新日期：2004-08-24 00:00:00

abstract：:An unstable allele of the bronze 2 (bz2) locus was isolated from a Robertson's Mutator Zea mays line containing a family of active transposable Mu elements. This mutation is somatically unstable, resulting in numerous revertant purple sectors on a bronze kernel. By following the variegated kernel phenotype through two...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chandler VL,Walbot V

更新日期：1986-03-01 00:00:00

abstract：:Persisting (P) cells (murine cells that resemble mast cells and grow continuously in vitro for prolonged periods in the presence of a specific growth factor) did not express detectable levels of Ia antigens (murine class II major histocompatibility antigens) when their growth was supported by partially purified P cell...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wong GH,Clark-Lewis I,McKimm-Breschkin JL,Schrader JW

更新日期：1982-11-01 00:00:00

abstract：:ALOX15 (12/15-lipoxygenase) orthologs have been implicated in maturational degradation of intracellular organelles and in the biosynthesis of antiinflammatory and proresolving eicosanoids. Here we hypothesized that lower mammals (mice, rats, pigs) express 12-lipoxygenating ALOX15 orthologs. In contrast, 15-lipoxygenat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Adel S,Karst F,González-Lafont À,Pekárová M,Saura P,Masgrau L,Lluch JM,Stehling S,Horn T,Kuhn H,Heydeck D

更新日期：2016-07-26 00:00:00

abstract：:Loss of IκB kinase (IKK) β-dependent NF-κB signaling in hematopoietic cells is associated with increased granulopoiesis. Here we identify a regulatory cytokine loop that causes neutrophilia in Ikkβ-deficient mice. TNF-α-dependent apoptosis of myeloid progenitor cells leads to the release of IL-1β, which promotes Th17 ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mankan AK,Canli O,Schwitalla S,Ziegler P,Tschopp J,Korn T,Greten FR

更新日期：2011-04-19 00:00:00

abstract：:Abnormal H2O2 levels are closely related to many diseases, including inflammation and cancers. Herein, we simultaneously load HRP and its substrate, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), into liposomal nanoparticles, obtaining a Lipo@HRP&ABTS optical nanoprobe for in vivo H2O2-responsive chrom...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen Q,Liang C,Sun X,Chen J,Yang Z,Zhao H,Feng L,Liu Z

更新日期：2017-05-23 00:00:00

abstract：:The Miocene is characterized by a series of key climatic events that led to the founding of the late Cenozoic icehouse mode and the dawn of modern biota. The processes that caused these developments, and particularly the role of atmospheric CO2 as a forcing factor, are poorly understood. Here we present a CO2 record b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kürschner WM,Kvacek Z,Dilcher DL

更新日期：2008-01-15 00:00:00

abstract：:Transmission electron microscopy studies have been used to argue that magnetite crystals in carbonate from Martian meteorite ALH84001 have a composition and morphology indistinguishable from that of magnetotactic bacteria. It has even been claimed from scanning electron microscopy imaging that some ALH84001 magnetite ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weiss BP,Kim SS,Kirschvink JL,Kopp RE,Sankaran M,Kobayashi A,Komeili A

更新日期：2004-06-01 00:00:00

abstract：:The elucidation of the mechanisms of antigen presentation by major histocompatibility complex class I molecules has stimulated the search for nonreplicative vectors that could deliver CD8+ T cell epitopes to the cytosol of antigen-presenting cells to trigger the activation of specific cytotoxic T lymphocytes (CTLs) in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Saron MF,Fayolle C,Sebo P,Ladant D,Ullmann A,Leclerc C

更新日期：1997-04-01 00:00:00

abstract：:It is suggested that active transport, muscle contraction, and ribosomal translocation may all make use of a common allosteric mechanism in which ATP or GTP serves as both the effector and substrate and in which a conformational change in a protein (enzyme) moves or exerts a force on a second ligand. The enzymatic spl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hill TL

更新日期：1969-09-01 00:00:00

abstract：:We describe a physical map of the end of the long arm of the human X chromosome encompassing the region from Xq27.2 to the q telomere, inclusive of the chromosomal band Xq28. This region is of particular interest, since it contains the highest density of genes associated with genetic diseases. The map covers a total o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Poustka A,Dietrich A,Langenstein G,Toniolo D,Warren ST,Lehrach H

更新日期：1991-10-01 00:00:00

abstract：:Programmed cell death regulates a number of biological phenomena, and the apoptotic signal must itself be tightly controlled to avoid inappropriate cell death. We established a genetic screen to search for molecules that inhibit the apoptotic signal from the Fas receptor. Here we report the isolation of a gene, LFG, t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Somia NV,Schmitt MJ,Vetter DE,Van Antwerp D,Heinemann SF,Verma IM

更新日期：1999-10-26 00:00:00

abstract：:Neuroblastoma-glioma hybrid cells (NG108-15) in suspension accumulate the permeant lipophilic cation [(3)H]tetraphenylphosphonium (TPP(+)) against a concentration gradient. The steady-state level of TPP(+) accumulation is about twice as great in physiological media of low K(+) concentration (i.e., 5 mM K(+)/135 mM Na(...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lichtshtein D,Kaback HR,Blume AJ

更新日期：1979-02-01 00:00:00

abstract：:We describe here the successful establishment of a reverse genetics system for rotavirus (RV), a member of the Reoviridae family whose genome consists of 10-12 segmented dsRNA. The system is based on the recombinant vaccinia virus T7 RNA polymerase-driven procedure for supplying artificial viral mRNA in the cytoplasm....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Komoto S,Sasaki J,Taniguchi K

更新日期：2006-03-21 00:00:00

abstract：:Hereditary hemochromatosis (HH) is a disorder of iron metabolism caused by common mutations in the gene HFE. The HFE protein binds to transferrin receptor-1 (TfR1) in competition with transferrin, and in vitro, reduces cellular iron by reducing iron uptake. However, in vivo, HFE is strongly expressed by liver macropha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Drakesmith H,Sweetland E,Schimanski L,Edwards J,Cowley D,Ashraf M,Bastin J,Townsend AR

更新日期：2002-11-26 00:00:00

abstract：:We established a pancreatic adenocarcinoma cell line (CFPAC-1) from a patient with cystic fibrosis (CF) and assessed some of its properties. The cells show epithelial morphology and express cytokeratin and oncofetal antigens characteristic of pancreatic duct cells. Basal and stimulated levels of cAMP and cAMP-dependen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schoumacher RA,Ram J,Iannuzzi MC,Bradbury NA,Wallace RW,Hon CT,Kelly DR,Schmid SM,Gelder FB,Rado TA

更新日期：1990-05-01 00:00:00

abstract：:Distinguishing tumor from normal glandular breast tissue is an important step in breast-conserving surgery. Because this distinction can be challenging in the operative setting, up to 40% of patients require an additional operation when traditional approaches are used. Here, we present a proof-of-concept study to dete...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Calligaris D,Caragacianu D,Liu X,Norton I,Thompson CJ,Richardson AL,Golshan M,Easterling ML,Santagata S,Dillon DA,Jolesz FA,Agar NY

更新日期：2014-10-21 00:00:00

abstract：:Bacillus thuringiensis is a widely used bacterial entomopathogen producing insecticidal toxins, some of which are expressed in insect-resistant transgenic crops. Surprisingly, the killing mechanism of B. thuringiensis remains controversial. In particular, the importance of the septicemia induced by the host midgut mic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Caccia S,Di Lelio I,La Storia A,Marinelli A,Varricchio P,Franzetti E,Banyuls N,Tettamanti G,Casartelli M,Giordana B,Ferré J,Gigliotti S,Ercolini D,Pennacchio F

更新日期：2016-08-23 00:00:00

abstract：:An enzymatic activity, having the properties expected of a B-specific host-controlled modification enzyme, has been purified from an extract of Escherichia coli strain B. This activity renders the unmodified replicative form of phage fd resistant to B-specific restriction and is only present in strains carrying intact...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kühnlein U,Linn S,Arber W

更新日期：1969-06-01 00:00:00

abstract：:The Drosophila melanogaster urate oxidase (UO)-encoding gene is expressed in the third-instar larva and adult. In contrast, the Drosophila pseudoobscura UO gene is only expressed in the adult, whereas the Drosophila virilis UO gene is expressed only in the third-instar larva. UO activity in these three Drosophila spec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wallrath LL,Friedman TB

更新日期：1991-07-01 00:00:00

abstract：:In this report we identify the specific isozymes of protein kinase C (PKC) that are involved in c-fos and c-jun mRNA accumulation in the rat basophilic leukemia cell line RBL-2H3. These cells could be largely depleted of the endogenous PKC isozymes by chronic treatment with phorbol 12-myristate 13-acetate followed by ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Razin E,Szallasi Z,Kazanietz MG,Blumberg PM,Rivera J

更新日期：1994-08-02 00:00:00