H2O2-responsive liposomal nanoprobe for photoacoustic inflammation imaging and tumor theranostics via in vivo chromogenic assay.

Abstract:

:Abnormal H2O2 levels are closely related to many diseases, including inflammation and cancers. Herein, we simultaneously load HRP and its substrate, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), into liposomal nanoparticles, obtaining a Lipo@HRP&ABTS optical nanoprobe for in vivo H2O2-responsive chromogenic assay with great specificity and sensitivity. In the presence of H2O2, colorless ABTS would be converted by HRP into the oxidized form with strong near-infrared (NIR) absorbance, enabling photoacoustic detection of H2O2 down to submicromolar concentrations. Using Lipo@HRP&ABTS as an H2O2-responsive nanoprobe, we could accurately detect the inflammation processes induced by LPS or bacterial infection in which H2O2 is generated. Meanwhile, upon systemic administration of this nanoprobe we realize in vivo photoacoustic imaging of small s.c. tumors (∼2 mm in size) as well as orthotopic brain gliomas, by detecting H2O2 produced by tumor cells. Interestingly, local injection of Lipo@HRP&ABTS further enables differentiation of metastatic lymph nodes from those nonmetastatic ones, based on their difference in H2O2 contents. Moreover, using the H2O2-dependent strong NIR absorbance of Lipo@HRP&ABTS, tumor-specific photothermal therapy is also achieved. This work thus develops a sensitive H2O2-responsive optical nanoprobe useful not only for in vivo detection of inflammation but also for tumor-specific theranostic applications.

authors

Chen Q,Liang C,Sun X,Chen J,Yang Z,Zhao H,Feng L,Liu Z

doi

10.1073/pnas.1701976114

subject

Has Abstract

pub_date

2017-05-23 00:00:00

pages

5343-5348

issue

21

eissn

0027-8424

issn

1091-6490

pii

1701976114

journal_volume

114

pub_type

杂志文章
  • Membrane lipid saturation activates endoplasmic reticulum unfolded protein response transducers through their transmembrane domains.

    abstract::Endoplasmic reticulum (ER) stress sensors use a related luminal domain to monitor the unfolded protein load and convey the signal to downstream effectors, signaling an unfolded protein response (UPR) that maintains compartment-specific protein folding homeostasis. Surprisingly, perturbation of cellular lipid compositi...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1217611110

    authors: Volmer R,van der Ploeg K,Ron D

    更新日期:2013-03-19 00:00:00

  • Alkynyl sugar analogs for the labeling and visualization of glycoconjugates in cells.

    abstract::Developing tools for investigating the cellular activity of glycans will help to delineate the molecular basis for aberrant glycosylation in pathological processes such as cancer. Metabolic oligosaccharide engineering, which inserts sugar-reporting groups into cellular glycoconjugates, represents a powerful method for...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0611307104

    authors: Hsu TL,Hanson SR,Kishikawa K,Wang SK,Sawa M,Wong CH

    更新日期:2007-02-20 00:00:00

  • Color naming reflects optimal partitions of color space.

    abstract::The nature of color categories in the world's languages is contested. One major view holds that color categories are organized around universal focal colors, whereas an opposing view holds instead that categories are defined at their boundaries by linguistic convention. Both of these standardly opposed views are chall...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0610341104

    authors: Regier T,Kay P,Khetarpal N

    更新日期:2007-01-23 00:00:00

  • Specificity of diffusion channels produced by lambda phage receptor protein of Escherichia coli.

    abstract::The lamB protein, the receptor for phage lambda, was purified from the outer membrane of Escherichia coli K-12 by extraction with Triton X-100 and EDTA, chromatography on DEAE-Sephacel in Triton X-100, exchange of Triton for cholate by gel filtration, and chromatography on Sephacryl S-200 in cholate, NaCl, and EDTA. T...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.77.1.167

    authors: Luckey M,Nikaido H

    更新日期:1980-01-01 00:00:00

  • Global temperature responses to current emissions from the transport sectors.

    abstract::Transport affects climate directly and indirectly through mechanisms that cause both warming and cooling of climate, and the effects operate on very different timescales. We calculate climate responses in terms of global mean temperature and find large differences between the transport sectors with respect to the size...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0804844105

    authors: Berntsen T,Fuglestvedt J

    更新日期:2008-12-09 00:00:00

  • Coastal landforms and accumulation of mangrove peat increase carbon sequestration and storage.

    abstract::Given their relatively small area, mangroves and their organic sediments are of disproportionate importance to global carbon sequestration and carbon storage. Peat deposition and preservation allows some mangroves to accrete vertically and keep pace with sea-level rise by growing on their own root remains. In this stu...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1519774113

    authors: Ezcurra P,Ezcurra E,Garcillán PP,Costa MT,Aburto-Oropeza O

    更新日期:2016-04-19 00:00:00

  • Halogen bonds in biological molecules.

    abstract::Short oxygen-halogen interactions have been known in organic chemistry since the 1950s and recently have been exploited in the design of supramolecular assemblies. The present survey of protein and nucleic acid structures reveals similar halogen bonds as potentially stabilizing inter- and intramolecular interactions t...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0407607101

    authors: Auffinger P,Hays FA,Westhof E,Ho PS

    更新日期:2004-11-30 00:00:00

  • Maternal mediation, stress inoculation, and the development of neuroendocrine stress resistance in primates.

    abstract::The stress inoculation hypothesis presupposes that brief intermittent stress exposure early in life induces the development of subsequent stress resistance in human and nonhuman primates. Rodent studies, however, suggest a role for maternal care rather than stress exposure per se (i.e., the maternal mediation hypothes...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0506571103

    authors: Parker KJ,Buckmaster CL,Sundlass K,Schatzberg AF,Lyons DM

    更新日期:2006-02-21 00:00:00

  • Selective loss of wheat germ agglutinin binding to agglutinin-resistant mutants of Chinese hamster ovary cells.

    abstract::The binding of 125I-labeled wheat germ agglutinin (WGA) to parental and three distinct WGA-resistant Chinese hamster ovary cell lines possessing modified cell surface carbohydrate structures has been examined over a 10(6)-fold range of WGA concentrations. The Scatchard plot for WGA binding to parental cells was comple...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.74.11.5056

    authors: Stanley P,Carver JP

    更新日期:1977-11-01 00:00:00

  • Biased learning affects mate choice in a butterfly.

    abstract::Early acquisition of mate preferences or mate-preference learning is associated with signal diversity and speciation in a wide variety of animal species. However, the diversity of mechanisms of mate-preference learning across taxa remains poorly understood. Using the butterfly Bicyclus anynana we uncover a mechanism t...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1118378109

    authors: Westerman EL,Hodgins-Davis A,Dinwiddie A,Monteiro A

    更新日期:2012-07-03 00:00:00

  • Glucose induces two amino acid transport systems in Chlorella.

    abstract::In autotrophically grown Chlorella cells, glucose induces a hexose transport system but, at the same time, the synthesis of two amino acid transport systems is also induced. Thus, the rates of uptake of glycine, L-alanine, L-proline, and L-serine, all of which compete with each other for entry into the cells, increase...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.78.6.3591

    authors: Cho BH,Sauer N,Komor E,Tanner W

    更新日期:1981-06-01 00:00:00

  • X chromosome and autosomal recombination are differentially sensitive to disruptions in SC maintenance.

    abstract::The synaptonemal complex (SC) is a conserved meiotic structure that regulates the repair of double-strand breaks (DSBs) into crossovers or gene conversions. The removal of any central-region SC component, such as the Drosophila melanogaster transverse filament protein C(3)G, causes a complete loss of SC structure and ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1910840116

    authors: Billmyre KK,Cahoon CK,Heenan GM,Wesley ER,Yu Z,Unruh JR,Takeo S,Hawley RS

    更新日期:2019-10-22 00:00:00

  • A putative ATP-dependent RNA helicase involved in Saccharomyces cerevisiae ribosome assembly.

    abstract::We have isolated a cold-sensitive mutant of Saccharomyces cerevisiae in which there is a deficit of 60S ribosomal subunits. Cold sensitivity and the assembly defect are recessive and cosegregate, defining a single essential gene that we designated DRS1 (deficiency of ribosomal subunits). The wild-type DRS1 gene was cl...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.89.23.11131

    authors: Ripmaster TL,Vaughn GP,Woolford JL Jr

    更新日期:1992-12-01 00:00:00

  • The peptide-catalyzed stereospecific synthesis of tetroses: a possible model for prebiotic molecular evolution.

    abstract::Using a water-based prebiotic model of sugar synthesis involving glycolaldehyde self-condensation, we demonstrate that homochiral L-dipeptide catalysts lead to the stereospecific syntheses of tetroses. The asymmetric effect is largest for erythrose, which may reach a D-enantiomeric excess of >80% with L-Val-L-Val cata...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0602320103

    authors: Weber AL,Pizzarello S

    更新日期:2006-08-22 00:00:00

  • Surface-wave-enabled darkfield aperture for background suppression during weak signal detection.

    abstract::Sensitive optical signal detection can often be confounded by the presence of a significant background, and, as such, predetection background suppression is substantively important for weak signal detection. In this paper, we present a novel optical structure design, termed surface-wave-enabled darkfield aperture (SWE...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0912563107

    authors: Zheng G,Cui X,Yang C

    更新日期:2010-05-18 00:00:00

  • Oligomeric structure of a prototype retrovirus glycoprotein.

    abstract::The structure of the Rous sarcoma virus envelope glycoprotein complex was studied by sedimentation gradient centrifugation analyses of detergent-solubilized wild-type and mutant envelope (env) gene products. These studies show that the envelope glycoprotein forms an oligomer during biosynthesis, which is most likely a...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.85.22.8688

    authors: Einfeld D,Hunter E

    更新日期:1988-11-01 00:00:00

  • Facebook language predicts depression in medical records.

    abstract::Depression, the most prevalent mental illness, is underdiagnosed and undertreated, highlighting the need to extend the scope of current screening methods. Here, we use language from Facebook posts of consenting individuals to predict depression recorded in electronic medical records. We accessed the history of Faceboo...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1802331115

    authors: Eichstaedt JC,Smith RJ,Merchant RM,Ungar LH,Crutchley P,Preoţiuc-Pietro D,Asch DA,Schwartz HA

    更新日期:2018-10-30 00:00:00

  • Translocation of molecules into cells by pH-dependent insertion of a transmembrane helix.

    abstract::We have previously observed the spontaneous, pH-dependent insertion of a water-soluble peptide to form a helix across lipid bilayers [Hunt, J. F., Rath, P., Rothschild, K. J. & Engelman, D. M. (1997) Biochemistry 36, 15177-15192]. We now use a related peptide, pH (low) insertion peptide, to translocate cargo molecules...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0601463103

    authors: Reshetnyak YK,Andreev OA,Lehnert U,Engelman DM

    更新日期:2006-04-25 00:00:00

  • On a classical limit for electronic degrees of freedom that satisfies the Pauli exclusion principle.

    abstract::Fermions need to satisfy the Pauli exclusion principle: no two can be in the same state. This restriction is most compactly expressed in a second quantization formalism by the requirement that the creation and annihilation operators of the electrons satisfy anticommutation relations. The usual classical limit of quant...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.97.5.1965

    authors: Levine RD

    更新日期:2000-02-29 00:00:00

  • Plasma membrane/cell wall perturbation activates a novel cell cycle checkpoint during G1 in Saccharomyces cerevisiae.

    abstract::Cellular wound healing or the repair of plasma membrane/cell wall damage (plasma membrane damage) occurs frequently in nature. Although various cellular perturbations, such as DNA damage, spindle misalignment, and impaired daughter cell formation, are monitored by cell cycle checkpoint mechanisms in budding yeast, whe...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1523824113

    authors: Kono K,Al-Zain A,Schroeder L,Nakanishi M,Ikui AE

    更新日期:2016-06-21 00:00:00

  • Functional characterization of bacterial sRNAs using a network biology approach.

    abstract::Small RNAs (sRNAs) are important components of posttranscriptional regulation. These molecules are prevalent in bacterial and eukaryotic organisms, and involved in a variety of responses to environmental stresses. The functional characterization of sRNAs is challenging and requires highly focused and extensive experim...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1104318108

    authors: Modi SR,Camacho DM,Kohanski MA,Walker GC,Collins JJ

    更新日期:2011-09-13 00:00:00

  • Impaired attentional selection following lesions to human pulvinar: evidence for homology between human and monkey.

    abstract::We examined the contributions of the human pulvinar to goal directed selection of visual targets in 3 patients with chronic, unilateral lesions involving topographic maps in the ventral pulvinar. Observers completed 2 psychophysical tasks in which they discriminated the orientation of a lateralized target grating in t...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 临床试验,杂志文章

    doi:10.1073/pnas.0810086106

    authors: Snow JC,Allen HA,Rafal RD,Humphreys GW

    更新日期:2009-03-10 00:00:00

  • Molecular cloning and expression of an additional epidermal growth factor receptor-related gene.

    abstract::Epidermal growth factor (EGF), transforming growth factor alpha (TGF-alpha), and amphiregulin are structurally and functionally related growth regulatory proteins. These secreted polypeptides all bind to the 170-kDa cell-surface EGF receptor, activating its intrinsic kinase activity. However, amphiregulin exhibits dif...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.87.13.4905

    authors: Plowman GD,Whitney GS,Neubauer MG,Green JM,McDonald VL,Todaro GJ,Shoyab M

    更新日期:1990-07-01 00:00:00

  • Catalytic asymmetric addition of diphenylzinc to cyclic alpha,beta-unsaturated ketones.

    abstract::We have examined the use of our bis(sulfonamide) diol ligand (1) in the asymmetric addition of phenyl groups to cyclic alpha,beta-unsaturated ketones. Good to excellent enantioselectivities have been obtained with cyclic enones bearing alkyl substituents in the 2 position (71-97% enantiomeric excess). Furthermore, exc...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0307119101

    authors: Li H,García C,Walsh PJ

    更新日期:2004-04-13 00:00:00

  • F901318 represents a novel class of antifungal drug that inhibits dihydroorotate dehydrogenase.

    abstract::There is an important medical need for new antifungal agents with novel mechanisms of action to treat the increasing number of patients with life-threatening systemic fungal disease and to overcome the growing problem of resistance to current therapies. F901318, the leading representative of a novel class of drug, the...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1608304113

    authors: Oliver JD,Sibley GEM,Beckmann N,Dobb KS,Slater MJ,McEntee L,du Pré S,Livermore J,Bromley MJ,Wiederhold NP,Hope WW,Kennedy AJ,Law D,Birch M

    更新日期:2016-11-08 00:00:00

  • Anacardic acid induces IL-33 and promotes remyelination in CNS.

    abstract::Given the known neuroreparative actions of IL-33 in experimental models of central nervous system (CNS) injury, we predicted that compounds which induce IL-33 are likely to promote remyelination. We found anacardic acid as a candidate molecule to serve as a therapeutic agent to promote remyelination. Addition of anaca...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.2006566117

    authors: Ljunggren-Rose Å,Natarajan C,Matta P,Pandey A,Upender I,Sriram S

    更新日期:2020-09-01 00:00:00

  • Host factor Hfq of Escherichia coli stimulates elongation of poly(A) tails by poly(A) polymerase I.

    abstract::Current evidence suggests that the length of poly(A) tails of bacterial mRNAs result from a competition between poly(A) polymerase and exoribonucleases that attack the 3' ends of RNAs. Here, we show that host factor Hfq is also involved in poly(A) tail metabolism. Inactivation of the hfq gene reduces the length of pol...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.040549897

    authors: Hajnsdorf E,Régnier P

    更新日期:2000-02-15 00:00:00

  • Synergy of aromatic residues and phosphoserines within the intrinsically disordered DNA-binding inhibitory elements of the Ets-1 transcription factor.

    abstract::The E26 transformation-specific (Ets-1) transcription factor is autoinhibited by a conformationally disordered serine-rich region (SRR) that transiently interacts with its DNA-binding ETS domain. In response to calcium signaling, autoinhibition is reinforced by calmodulin-dependent kinase II phosphorylation of serines...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1401891111

    authors: Desjardins G,Meeker CA,Bhachech N,Currie SL,Okon M,Graves BJ,McIntosh LP

    更新日期:2014-07-29 00:00:00

  • Withdrawal of IL-7 induces Bax translocation from cytosol to mitochondria through a rise in intracellular pH.

    abstract::IL-7 functions as a trophic factor during T lymphocyte development by a mechanism that is partly based on the induction of Bcl-2, which protects cells from apoptosis. Here we report a mechanism by which cytokine withdrawal activates the prodeath protein Bax. On loss of IL-7 in a dependent cell line, Bax protein transl...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.96.25.14476

    authors: Khaled AR,Kim K,Hofmeister R,Muegge K,Durum SK

    更新日期:1999-12-07 00:00:00

  • neurogenin2 elicits the genesis of retinal neurons from cultures of nonneural cells.

    abstract::neurogenin2 (ngn2) encodes a basic helix-loop-helix transcription factor and plays an important role in neurogenesis from migratory neural crest cells. Its role in retinal development is poorly understood. We observed that in the developing chick retina, ngn2 was expressed in a subpopulation of proliferating progenito...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.261455698

    authors: Yan RT,Ma WX,Wang SZ

    更新日期:2001-12-18 00:00:00