The catalytic core of DEMETER guides active DNA demethylation in Arabidopsis.

abstract：:Fast axoplasmic transport was studied in dorsal root ganglion cells of the cat. Proteins carried in the fast axoplasmic flow were labeled after an intraganglionic injection of (L)-[4,5-(3)H]leucine. The rate of transport was 380 +/- 26 mm/day in both the central and peripheral branches of the bifurcating axons that ar...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Anderson LE,McClure WO

更新日期：1973-05-01 00:00:00

abstract：:Prolonged incubation of NIH 3T3 cells under the growth constraint of confluence results in a persistent impairment of proliferation when the cells are subcultured at low density and a greatly increased probability of neoplastic transformation in assays for transformation. These properties, along with the large accumul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chow M,Rubin H

更新日期：1996-09-03 00:00:00

abstract：:Proteasome activator PA200 enhances proteasome-mediated cleavage after acidic residues in vitro; however, its role within cells is not known. Here, we show that, in response to ionizing radiation, PA200 forms hybrid proteasomes with 19S caps and 20S core proteasomes that accumulate on chromatin, leading to an increase...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Blickwedehl J,Agarwal M,Seong C,Pandita RK,Melendy T,Sung P,Pandita TK,Bangia N

更新日期：2008-10-21 00:00:00

abstract：:All immunoglobulins and T-cell receptors throughout phylogeny share regions of highly conserved amino acid sequence. To identify possible primitive immunoglobulins and immunoglobulin-like molecules, we utilized 3' RACE (rapid amplification of cDNA ends) and a highly conserved constant region consensus amino acid seque...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berstein RM,Schluter SF,Shen S,Marchalonis JJ

更新日期：1996-04-16 00:00:00

abstract：:Transporters in the Golgi apparatus membrane translocate nucleotide sugars from the cytosol into the Golgi lumen before these can be substrates for the glycosylation of proteins, lipids, and proteoglycans. We have cloned the mammalian Golgi membrane transporter for uridine diphosphate-N-acetylglucosamine by phenotypic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guillen E,Abeijon C,Hirschberg CB

更新日期：1998-07-07 00:00:00

abstract：:RNA synthesis by the paramyxovirus respiratory syncytial virus, a ubiquitous human pathogen, was found to be more complex than previously appreciated for the nonsegmented negative-strand RNA viruses. Intracellular RNA replication of a plasmid-encoded "minigenome" analog of viral genomic RNA was directed by coexpressio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Collins PL,Hill MG,Cristina J,Grosfeld H

更新日期：1996-01-09 00:00:00

abstract：:A method using PCR amplification and primer extension with fluorescent oligonucleotides was developed to analyze T-cell repertoires. The sizes of the hypervariable CDR3-like regions of the murine T-cell antigen receptor beta chains were measured for all possible V beta-J beta combinations. This analysis shows that bet...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pannetier C,Cochet M,Darche S,Casrouge A,Zöller M,Kourilsky P

更新日期：1993-05-01 00:00:00

abstract：:Harvey and Kirsten murine sarcoma viruses each encode a structurally and functionally related 21-kilodalton protein (p21), which is the transforming protein of each virus. Using probes from the 0.9-kilobase (kb) p21-coding region of each virus (called v-Ha-ras and v-Ki-ras, respectively), we have molecularly cloned fr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chang EH,Gonda MA,Ellis RW,Scolnick EM,Lowy DR

更新日期：1982-08-01 00:00:00

abstract：:Hereditary deficiency of factor IXa (fIXa), a key enzyme in blood coagulation, causes hemophilia B, a severe X chromosome-linked bleeding disorder afflicting 1 in 30,000 males; clinical studies have identified nearly 500 deleterious variants. The x-ray structure of porcine fIXa described here shows the atomic origins ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brandstetter H,Bauer M,Huber R,Lollar P,Bode W

更新日期：1995-10-10 00:00:00

abstract：:We used gene knockout mice to explore the role of Angiopoietin-like-4 (Angptl4) in lipid metabolism as well as to generate anti-Angptl4 mAbs with pharmacological activity. Angptl4 -/- mice had lower triglyceride (TG) levels resulting both from increased very low-density lipoprotein (VLDL) clearance and decreased VLDL ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Desai U,Lee EC,Chung K,Gao C,Gay J,Key B,Hansen G,Machajewski D,Platt KA,Sands AT,Schneider M,Van Sligtenhorst I,Suwanichkul A,Vogel P,Wilganowski N,Wingert J,Zambrowicz BP,Landes G,Powell DR

更新日期：2007-07-10 00:00:00

abstract：:In vertebrate retinal photoreceptors, photoisomerization of opsin-bound visual chromophore 11-cis-retinal to all-trans-retinal triggers phototransduction events. Regeneration of the chromophore is a critical step in restoring photoreceptors to their dark-adapted state. This regeneration process, called the retinoid cy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Golczak M,Kuksa V,Maeda T,Moise AR,Palczewski K

更新日期：2005-06-07 00:00:00

abstract：:During vertebrate brain development, the onset of neuronal differentiation is under strict temporal control. In the mammalian thalamus and other brain regions, neurogenesis is regulated also in a spatially progressive manner referred to as a neurogenetic gradient, the underlying mechanism of which is unknown. Here we ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Scholpp S,Delogu A,Gilthorpe J,Peukert D,Schindler S,Lumsden A

更新日期：2009-11-24 00:00:00

abstract：:The guanidinium group of arginine-166 has been postulated to act as an electrophilic species during phosphorylation of alkaline phosphatase. Its role could be either to stabilize the developing negative charge on the oxygen of the leaving group or the pentacoordinate transition state or to help bind the -PO2-3 group. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Butler-Ransohoff JE,Kendall DA,Kaiser ET

更新日期：1988-06-01 00:00:00

abstract：:An emergent viral pathogen termed severe fever with thrombocytopenia syndrome virus (SFTSV) is responsible for thousands of clinical cases and associated fatalities in China, Japan, and South Korea. Akin to other phleboviruses, SFTSV relies on a viral glycoprotein, Gc, to catalyze the merger of endosomal host and vira...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Halldorsson S,Behrens AJ,Harlos K,Huiskonen JT,Elliott RM,Crispin M,Brennan B,Bowden TA

更新日期：2016-06-28 00:00:00

abstract：:Many Drosophila non-long terminal repeat (LTR) retrotransposons actively transpose into internal, gene-rich regions of chromosomes but do not transpose onto chromosome ends. HeT-A and TART are remarkable exceptions; they form telomeres of Drosophila by repeated transpositions onto the ends of chromosomes and never tra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rashkova S,Karam SE,Pardue ML

更新日期：2002-03-19 00:00:00

abstract：:In skeletal muscle, dihydropyridine receptors are functionally coupled to ryanodine receptors of the sarcoplasmic reticulum in triadic or diadic junctional complexes. In cardiac muscle direct physical or functional couplings have not been demonstrated. We have tested the hypothesis of functional coupling of L-type Ca2...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sham JS,Cleemann L,Morad M

更新日期：1995-01-03 00:00:00

abstract：:A survey was performed to estimate the frequency of enterohemorrhagic Escherichia coli O157:H7 or O157:nonmotile (EHEC O157) in feces and on hides within groups of fed cattle from single sources (lots) presented for slaughter at meat processing plants in the Midwestern United States, as well as frequency of carcass co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Elder RO,Keen JE,Siragusa GR,Barkocy-Gallagher GA,Koohmaraie M,Laegreid WW

更新日期：2000-03-28 00:00:00

abstract：:Devastating floods due to Atlantic hurricanes are relatively rare events. However, the frequency of the most intense storms is likely to increase with rises in sea surface temperatures. Geoengineering by stratospheric sulfate aerosol injection cools the tropics relative to the polar regions, including the hurricane Ma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Moore JC,Grinsted A,Guo X,Yu X,Jevrejeva S,Rinke A,Cui X,Kravitz B,Lenton A,Watanabe S,Ji D

更新日期：2015-11-10 00:00:00

abstract：:AK032317 is the GenBank accession no. of a full-length RIKEN mouse cDNA. It encodes a putative variant of the C3-type TRPC (transient receptor potential channel) that differs from the previously cloned murine TRPC3 cDNA in that it has a 5' extension stemming from inclusion of an additional exon (exon 0). The extended ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yildirim E,Kawasaki BT,Birnbaumer L

更新日期：2005-03-01 00:00:00

abstract：:Carbon monoxide (CO) synthesized by heme oxygenase 2 (HO2) and nitric oxide (NO) produced by neuronal NO synthase (nNOS) mediate nonadrenergic/noncholinergic (NANC) intestinal relaxation. In many areas of the gastrointestinal tract, NO and CO function as coneurotransmitters. In the internal anal sphincter (IAS), NANC ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Watkins CC,Boehning D,Kaplin AI,Rao M,Ferris CD,Snyder SH

更新日期：2004-02-24 00:00:00

abstract：:During mammalian aging, cellular proteins become increasingly damaged: for example, by carbonylation and formation of advanced glycation end products (AGEs). The means to ensure that offspring are born without such damage are unknown. Unexpectedly, we found that undifferentiated mouse ES cells contain high levels of b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hernebring M,Brolén G,Aguilaniu H,Semb H,Nyström T

更新日期：2006-05-16 00:00:00

abstract：:The empirical criteria for defining a clinical subtype of lung cancer are gradually transiting from histopathology to genetic variations in driver genes. Targeting these driver mutations, such as sensitizing epidermal growth factor receptor (EGFR) mutations, has dramatically improved the prognosis of advanced non-smal...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang Z,Shen Z,Li Z,Duan J,Fu S,Liu Z,Bai H,Zhang Z,Zhao J,Wang X,Wang J

更新日期：2015-08-11 00:00:00

abstract：:RNA movements and localization pervade biology, from embryonic development to disease. To identify RNAs at specific locations, we developed a strategy in which a uridine-adding enzyme is anchored to subcellular sites, where it directly marks RNAs with 3' terminal uridines. This localized RNA recording approach yields ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Medina-Munoz HC,Lapointe CP,Porter DF,Wickens M

更新日期：2020-09-22 00:00:00

abstract：:Microtubule assembly is enhanced by the addition of 1 M sucrose or 4 M glycerol to the reassembly mixture. Tubulin can be purified from guinea pig brain readily and in good yield by two cycles of assembly in glycerol-containing solutions. The tubules assembled in glycerol and sucrose are more stable than tubules forme...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shelanski ML,Gaskin F,Cantor CR

更新日期：1973-03-01 00:00:00

abstract：:Nutrition recommendations worldwide emphasize ingestion of plant-based diets rather than diets that rely primarily on animal products. However, this plant-based diet could limit the intake of essential nutrients such as calcium. Osteoporosis is one of the world's most prevalent nutritional disorders, and inadequate di...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morris J,Hawthorne KM,Hotze T,Abrams SA,Hirschi KD

更新日期：2008-02-05 00:00:00

abstract：:What drives scientists' position taking on matters where empirical answers are unavailable or contradictory? We examined the contentious debate on whether to limit experiments involving the creation of potentially pandemic pathogens. Hundreds of scientists, including Nobel laureates, have signed petitions on the debat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Edelmann A,Moody J,Light R

更新日期：2017-06-13 00:00:00

abstract：:The primary structure of the precursor of urotensin I, a neuropeptide hormone from the caudal neurosecretory system of the carp Cyprinus carpio, has been determined by analyzing the nucleotide sequence of cloned DNA complementary to the mRNA encoding it. The precursor consists of 145 amino acid residues and the carbox...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ishida I,Ichikawa T,Deguchi T

更新日期：1986-01-01 00:00:00

abstract：:Variable regions of Ig chains provide the antigen recognition portion of B-cell receptors and derivative antibodies. Ig heavy-chain variable region exons are assembled developmentally from V, D, J gene segments. Each variable region contains three antigen-contacting complementarity-determining regions (CDRs), with CDR...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hwang JK,Wang C,Du Z,Meyers RM,Kepler TB,Neuberg D,Kwong PD,Mascola JR,Joyce MG,Bonsignori M,Haynes BF,Yeap LS,Alt FW

更新日期：2017-08-08 00:00:00

abstract：:The role of the serum proteolytic system plasminogen/plasminogen activator as a biochemical tool used by the glia or neurons, or both, for maintaining their temporary and flexible cellular interactions during histogenesis of the nervous system is under study. The present report identifies a glia cell type, the Schwann...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kalderon N

更新日期：1984-11-01 00:00:00

abstract：:Tomato flavor is dependent upon a complex mixture of volatiles including multiple acetate esters. Red-fruited species of the tomato clade accumulate a relatively low content of acetate esters in comparison with the green-fruited species. We show that the difference in volatile ester content between the red- and green-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goulet C,Mageroy MH,Lam NB,Floystad A,Tieman DM,Klee HJ

更新日期：2012-11-13 00:00:00