Abstract：
:Chalcones are phenolic compounds that can be isolated from plants. Previous studies have described some pharmacological applications for these compounds. Making use of our established reporter gene system, we determined the effect of five hydroxychalcones--2-hydroxychalcone, 2'-hydroxychalcone, 4-hydroxychalcone, 4,2',4'-trihydroxychalcone, and 3,4,2',4'-tetrahydroxychalcone--on the cellular xenobiotic responsive element (XRE)-transactivation. The interference of chalcones acting against polycyclic aromatic hydrocarbon (PAH)-DNA binding was also examined. Enzyme inhibition assays of cytochrome P450 (CYP) 1A1 and CYP1B1 were initially performed on recombinant protein expressed in insect microsomes. 2'-Hydroxychalcone and 2-hydroxychalcone were the most effective among the tested hydroxychalcones. The two hydroxychalcones had comparable IC50 values for CYP1A1 and CYP1B1, which were determined to be at the micromolar and submicromolar range, respectively. However, reporter gene assays indicated that 2'-hydroxychalcone suppressed XRE-transactivation, whereas 2-hydroxychalcone induced it when 7,12-dimethylbenz[a]anthracene (DMBA) was co-administered. In the absence of DMBA, 10 microM 2-hydroxychalcone and 2'-hydroxychalcone increased XRE-transactivation by 18- and 2.5-fold, respectively, while other chalcones did not significantly alter the response. Cultures treated with the two hydroxychalcones also displayed separate trends in ethoxyresorufin-O-deethylase (EROD) activity and DMBA-DNA covalent binding. In summary, the present study illustrated that the inhibition of hydroxychalcone on CYP1 enzymes and XRE-transactivation was affected by the position and number of hydroxyl groups in its structure.
journal_name
Toxicologyjournal_title
Toxicologyauthors
Wang H,Wang Y,Chen ZY,Chan FL,Leung LKdoi
10.1016/j.tox.2004.10.005keywords:
subject
Has Abstractpub_date
2005-02-14 00:00:00pages
303-13issue
2eissn
0300-483Xissn
1879-3185pii
S0300-483X(04)00586-4journal_volume
207pub_type
杂志文章相关文献
TOXICOLOGY文献大全abstract：:The regional distribution of metallothionein (MT), zinc and copper was measured in brains of transgenic MT-I overexpressor (MT-I*) mice, MT-I/MT-II gene knockout (MT-I/MT-II null) mice, and in brains of control C57BL/6J mice with normal MT expression. Toxic milk (tx) mutant mice with abnormally high MT and copper accu...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/s0300-483x(97)00133-9
更新日期：1997-12-19 00:00:00
abstract：:Recent Canadian research efforts have been directed towards the development of a reactive skin decontaminant (RSD) lotion active against classical nerve agents and mustard. The formulation presently under study consists of a 1.25 molal solution of potassium 2,3-butanedione monoximate (KBDO) in polyethylene glycol meth...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(91)90028-y
更新日期：1991-05-01 00:00:00
abstract：:Transgenic rat mutation assays can be used to assess genotoxic properties of chemicals in target organs for carcinogenicity. Mutations in transgenes are genetically neutral and accumulate during a treatment period; thus, assays are suitable for assessing the genotoxic risk of chemicals using a repeated-dose treatment ...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2014.07.003
更新日期：2014-10-03 00:00:00
abstract：:A number of studies reported that the MTS in vitro cytotoxicity assay is a convenient method for assessing cell viability. The main features found with this assay are its ease of use, accuracy and rapid indication of toxicity. It might well be a useful tool in human health risk assessment if it can be shown that this ...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/s0300-483x(97)00151-0
更新日期：1997-12-31 00:00:00
abstract：:4-Amino-2,6-dichlorophenol (ADCP) is a potent acute nephrotoxicant in vivo inducing prominent renal corticomedullary necrosis. In vitro, ADCP exposure increases lactate dehydrogenase (LDH) release from rat renal cortical slices at 0.05 mM or greater. The purpose of this study was to examine the ability of antioxidants...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2007.12.014
更新日期：2008-03-12 00:00:00
abstract：:Tobacco smoking is the largest preventable risk factor for morbidity and mortality in industrialized countries. WHO estimates that tobacco will become the largest single health problem by 2020, causing an estimated 8.4 million deaths annually. Tobacco has central importance in the etiology of cancers of the lung, head...
journal_title：Toxicology
pub_type： 杂志文章,评审
doi：10.1016/s0300-483x(01)00445-0
更新日期：2001-09-14 00:00:00
abstract：:To investigate the mechanism of hepatocarcinogenesis due to dicyclanil (DC), an insect growth regulator for sheep, histopathological and molecular biological analyses were performed in the liver of male ICR mice fed on a diet containing 1500 ppm of DC for 2 weeks (Experiment I; Exp. I). In gene expression analyses usi...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2004.10.011
更新日期：2005-02-28 00:00:00
abstract：:The development of mouse embryos was studied after intraperitoneal injection of nickel chloride in the preimplantation period. A single intraperitoneal injection of NiCl2 . 6H2O in 0.154 M NaCl corresponding to 20 mg/kg body wt was given to groups of female mice on days 1, 2, 3, 4, 5 or 6 of gestation. Control groups ...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(81)90104-9
更新日期：1981-01-01 00:00:00
abstract：:The standard treatment of poisoning by organophosphorus (OP) nerve agents with atropine and oximes is not sufficiently effective against all types of nerve agents. Alternative therapeutic strategies are required and bispyridinium non-oximes, acting as nicotinic antagonists, were identified as promising compounds. A pr...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2012.02.002
更新日期：2012-04-11 00:00:00
abstract：:The coumarin 7-hydroxylase of mice (Coh, CYP2A5) is known to be highly selectively inducible by both a set of heavy metals such as cobalt, indium and cerium and a variety of organic nitrogen-containing heteroaromatic compounds such as 3-amino-1,2,4-triazole, pyrazine and pyrazole. The investigations presented reveal t...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(95)03285-n
更新日期：1996-04-15 00:00:00
abstract：:Cyclosporine (CsA) is a universally used immunosuppressive drug which induces adverse side effects in several organs, but its impact on the heart is still controversial. Small heat shock proteins (sHSPs), such as HSP25 and alpha B-crystallin, are cytoprotective stress proteins exceptionally represented in the heart. T...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2009.06.007
更新日期：2009-08-21 00:00:00
abstract：:Many models have been developed to predict the combined effect of drugs and chemicals. Most models are classified into two additive models: independent action (IA) and concentration addition (CA). It is generally considered if the modes of action of chemicals are similar then the combined effect obeys CA; however, man...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2015.06.008
更新日期：2015-09-01 00:00:00
abstract：:The action of various manganese organic compounds, which are structural analogs of methylcyclopentadienyl manganese tricarbonyl (MMT), was investigated. Only the cyclopentadienyl manganese tricarbonyl compounds are effective inhibitors of mitochondrial respiration, but only when associated with NAD+-linked substrates....
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(77)90001-4
更新日期：1977-10-01 00:00:00
abstract：:Exposure of cultured rat mesangial cells to CdCl2 caused a dose- and time-dependent increase in intracellular glutathione that was significant at 0.5 microM and maximal at 1 microM Cd2+. The effect depended on glutathione synthesis and was masked by inhibiting synthesis with buthionine sulfoximine. The cells responded...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(93)90145-i
更新日期：1993-01-29 00:00:00
abstract：:This paper looks at toxicology digital sources produced and available in the United Kingdom (UK). It includes products aggregating a number of sources, which may include information from other countries, and for the convenience of the reader and information seeker these have also been described. ...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/s0300-483x(03)00198-7
更新日期：2003-08-21 00:00:00
abstract：:Both catecholamine (CA) released from adrenal chromaffin cells and hydrogen sulfide (H2S) have been shown to play critical roles in the regulation of hypoxic stress response. Our previous study has demonstrated that exogenous H2S directly induced quantal CA released from adult rat adrenal chromaffin cells (ARACCs) by ...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2013.06.014
更新日期：2013-10-04 00:00:00
abstract：:We have previously shown that young guinea pigs repeatedly exposed to sidestream cigarette smoke (SS) develop decreased airway reactivity of the C-fiber system without changing reactivity to one of its neurotransmitters, substance P (SP). This study was designed to determine whether the decreased reactivity was due to...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(96)03403-8
更新日期：1996-09-02 00:00:00
abstract：:The influence of pretreatment with the drug mixture (pyridostigmine, benactyzine and trihexyphenidyle), and antidotal treatment (the oxime HI-6 in combination with benactyzine) on respiration, circulation and survival of experimental animals poisoned with supralethal doses of soman (2 x LD50) was investigated in a rat...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/s0300-483x(97)00186-8
更新日期：1998-06-26 00:00:00
abstract：:Multidrug resistance type 1 P-glycoproteins (P-gp) and multidrug resistance associated proteins (MRP) were studied in differentiated primary human lung cells in culture, in comparison with permanent human lung cell lines and primary alveolar type II cells from rat lung. AII cells exhibited low basal levels of mdr1b mR...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/s0300-483x(01)00458-9
更新日期：2001-10-05 00:00:00
abstract：:The intravenous toxicity of phytic acid (inositol hexakisphosphate, IHP) has recently become of interest because of the potential for IHP incorporation into red blood cells to achieve a therapeutically useful shift in the hemoglobin-oxygen dissociation curve (Gersonde, K. and Nicolau, C., Blut, 39 (1979) 1). The obser...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(81)90022-6
更新日期：1981-01-01 00:00:00
abstract：:Malathion is one of the most widely used organophosphorus insecticides in agriculture. However, malathion may be involved in the etiology of human brain dysfunction. Induction of ROS has been proposed as a mechanism of malathion-induced poisoning cases, but there are few data regarding the effects of malathion on oxid...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2019.02.004
更新日期：2019-04-01 00:00:00
abstract：:Cyclophosphamide (CP) is a known immunomodulating agent. When presented to either late stage chick embryos (e.g. 18 days of incubation (DI] or neonatal chicks. CP induces selective B cell damage resulting in humoral immunosuppression in chickens. The present study was undertaken in order to provide further insights in...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(91)90196-8
更新日期：1991-03-11 00:00:00
abstract：:The distribution and excretion of radioactivity from [14C]citrinin (3 mg/kg, i.v) was determined in male rats. At 0.5 h after administration maximum values of 14.7% and 5.6% of total radioactivity were observed in the liver and kidneys, respectively, and by 6 h decreased to 7.5% in the liver and 4.7% in the kidney. Pl...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(79)90075-1
更新日期：1979-03-01 00:00:00
abstract：:Chlordecone (CD) impairs biliary excretion of organic anions (including phenolphthalein glucuronide (PG), imipramine polar metabolites, and taurocholate) without evidence of hepatocellular necrosis in rats. In this study we investigated the hypothesis that CD-induced hepatobiliary dysfunction is dependent on CD concen...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(94)90057-4
更新日期：1994-01-26 00:00:00
abstract：:Numerous dietary compounds, ubiquitous in fruits, vegetables and spices have been isolated and evaluated during recent years for their therapeutic potential. These compounds include flavonoid and non-flavonoid polyphenols, which describe beneficial effects against a variety of ailments. The notion that these plant pro...
journal_title：Toxicology
pub_type： 杂志文章,评审
doi：10.1016/j.tox.2009.11.008
更新日期：2010-11-28 00:00:00
abstract：:Rats were exposed to isopropanol by inhalation of 200,2000 and 8000 ppm for 2 weeks with a daily exposure of 6 h. A pilot group exposed to 8000 ppm was given a recovery period of 4 weeks. Kidney and liver microsomal metabolism of n-hexane was investigated in vitro concomitant with activities of cytochrome P-450 and GS...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(85)90078-2
更新日期：1985-01-01 00:00:00
abstract：:This paper presents an overview of issues related to surface water contamination arising from uranium mining activities in the Alligator Rivers Region (ARR) of northern Australia, and a program of research and monitoring that must assess the potential and actual effects on ecosystem and human health. The program of as...
journal_title：Toxicology
pub_type： 杂志文章,评审
doi：10.1016/s0300-483x(02)00470-5
更新日期：2002-12-27 00:00:00
abstract：:Dimethoate, azinphos-methyl, diazinon and pirimiphos-methyl, widely used organophosphorous insecticides, and benomyl, a benzimidazole fungicide, induce different cytotoxic effects on the human leukemia cell line HL-60. Among the insecticides tested, only azinphos and diazinon induced a dose-related inhibition of prote...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(94)90036-1
更新日期：1994-11-01 00:00:00
abstract：:Arsenic is an endocrine disruptor that promotes breast cancer (BCa) development. Estrogen synthesis, through aromatase activation, is essential for BCa promotion and progression through activating the G-coupled estrogen receptor 1 (GPER1), regulating rapid nongenomic effects involved in cell proliferation and migratio...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2020.152440
更新日期：2020-05-15 00:00:00
abstract：:The nerve agent tabun inhibits the essential enzyme acetylcholinesterase (AChE) by a rapid phosphoramidation of the catalytic serine residue. Oximes, such as K027 and HLö-7, can reactivate tabun-inhibited human AChE (tabun-hAChE) whereas the activity of their close structural analogue HI-6 is notably low. To investiga...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2009.09.002
更新日期：2009-11-30 00:00:00
