Abstract:
:To investigate the mechanism of hepatocarcinogenesis due to dicyclanil (DC), an insect growth regulator for sheep, histopathological and molecular biological analyses were performed in the liver of male ICR mice fed on a diet containing 1500 ppm of DC for 2 weeks (Experiment I; Exp. I). In gene expression analyses using a large-scale cDNA microarray and RT-PCR, fluctuations of expressions of metabolism-/oxidation-/reduction-related genes, such as CYP1A, aldehyde dehydrogenase family 1 subfamily A1 (Aldh1a1), and thioredoxin reductase 1 (Txnrd1), were predominantly observed in the liver of the DC-treated group. In Experiment II (Exp. II), small-scale and metabolism/oxidative stress-specific cDNA microarray, real-time RT-PCR, and measurement of NF-kappaB protein were performed in the mice liver using a two-stage hepatocarcinogenesis model, in which the male ICR mice were fed on a diet containing 1500 ppm of DC for 7 weeks after a single injection of dimethylnitrosamine (DMN). These mice were subjected to two-thirds partial hepatectomy (PH) at week 3. During histopathological examinations, a remarkable increase in gamma-glutamyltransferase-positive cells was observed in the DMN+DC+PH group. During the microarray and PCR analyses, the metabolism and oxidative stress-related genes, such as Cyp1a, P450 oxidoreductase (Por), and thioredoxin reductase 1 (Txnrd1); a few DNA damage/repair genes, such as 8-oxoguanine DNA-glycosylase 1 (Ogg1); and growth arrest and DNA-damage-inducible 45 alpha (Gadd45a), were fluctuated in this group, together with a slight increase in the concentration of activated NF-kappaB. These results suggest that DNA damages due to oxidative stress may be involved in the mechanism of DC-induced hepatocarcinogenesis in mice.
journal_name
Toxicologyjournal_title
Toxicologyauthors
Moto M,Okamura M,Muto T,Kashida Y,Machida N,Mistumori Kdoi
10.1016/j.tox.2004.10.011keywords:
subject
Has Abstractpub_date
2005-02-28 00:00:00pages
419-36issue
3eissn
0300-483Xissn
1879-3185pii
S0300-483X(04)00594-3journal_volume
207pub_type
杂志文章相关文献
TOXICOLOGY文献大全abstract::Ursodeoxycholic acid (UDCA) has been widely used for treating cholestatic liver diseases. However, in a recent review of clinical trial articles, its therapeutic benefits were not proven. Therefore, we investigated whether UDCA prevents or potentiates glycochenodeoxycholic acid (GCDCA)-induced apoptosis in isolated ra...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2005.05.025
更新日期:2005-10-15 00:00:00
abstract:OBJECTIVE:We investigated the potential dose related apoptotic effect of subchronic administration of the alkylating chemotherapeutic agent ifosfamide in different sites of the small and large rabbit intestine. MATERIALS AND METHODS:Twenty New Zealand White rabbits received intravenously every week, for 10 weeks, 0 (g...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2004.03.020
更新日期:2004-08-05 00:00:00
abstract::Troglitazone is a thiazolidinedione antidiabetic agent with insulin-sensitizing activities that was withdrawn from the market in 2000 due to its association with idiosyncratic hepatotoxicity. To address the suspected autoantibody production associated with troglitazone, we investigated autoantibodies in sera from pati...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2005.07.012
更新日期:2005-12-01 00:00:00
abstract::This review concerns some of the cell culture systems that are most frequently used in toxicology investigations. In particular, it sets out to evaluate the effectiveness of these cell culture systems in assessing the toxic potential of chemicals. Metabolic studies and general and specific toxicology investigations ar...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(81)90046-9
更新日期:1981-01-01 00:00:00
abstract::In the present study, we investigated the inducibility of the drug conjugate transporter genes MRP1 and MRP2 by redox-active compounds such as tertiary butylated hydroquinone (tBHQ) and quercetin and by chemicals known to activate the pregnane X receptor (PXR) such as rifampicin and clotrimazol and by the metalloid co...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(01)00570-4
更新日期:2002-02-28 00:00:00
abstract::In an attempt to assess the effect of ethanol on cadmium accumulation, metallothionein (MT) synthesis, Cd-binding capacity and lipid peroxidation, rats were administered either Cd, ethanol or their combination for a period of 4 weeks. A significant increase in Cd accumulation was observed in all the organs of rats und...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(92)90177-g
更新日期:1992-01-01 00:00:00
abstract::Butachlor is a systemic herbicide widely applied on rice, tea, wheat, beans and other crops; however, it concurrently exerts toxic effects on beneficial organisms like earthworms, aquatic invertebrates and other non-target animals including humans. Owing to the associated risk to humans, this chloroacetanilide class o...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2012.07.014
更新日期:2012-12-08 00:00:00
abstract::The effect of administration of a glutamate-containing protein hydrolysate on the arcuate nucleus of 10-day-old mice was studied by two methods. Arcuate nucleus damage resulted when administration was by a single large subcutaneous dose (100 ml/kg). When the same total dose was administered subcutaneously in five smal...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(75)90070-0
更新日期:1975-09-01 00:00:00
abstract::Rats were exposed to isopropanol by inhalation of 200,2000 and 8000 ppm for 2 weeks with a daily exposure of 6 h. A pilot group exposed to 8000 ppm was given a recovery period of 4 weeks. Kidney and liver microsomal metabolism of n-hexane was investigated in vitro concomitant with activities of cytochrome P-450 and GS...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(85)90078-2
更新日期:1985-01-01 00:00:00
abstract::The effect of tamoxifen on cytosolic free Ca2+ concentrations ([Ca2+]i) and viability has not been explored in corneal epithelial cells. This study examined whether tamoxifen altered [Ca2+]i and viability in SIRC corneal epithelial cells. Tamoxifen at concentrations > or = 1 microM increased [Ca2+]i in a concentration...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2008.10.001
更新日期:2009-01-08 00:00:00
abstract::Zinc (Zn) is an essential nutrient that is required in humans and animals for many physiological functions, including immune and antioxidant function, growth and reproduction. The present study was conducted to investigate the effects of adequate Zn level (38 mg/kg diet, as a control) and two low levels that create Zn...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(02)00049-5
更新日期:2002-06-14 00:00:00
abstract::The effect of amyl nitrite on the erythrocytes of adult and cord hemoglobin was examined in vitro. This study revealed that amyl nitrite caused oxyhemoglobin to become oxidized to methemoglobin wherein a rectangular hyperbolic curve was generated as the reaction progressed. This curve consisted of a reactionary log ph...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(85)90003-4
更新日期:1985-07-01 00:00:00
abstract::Gambogic acid (GA), a major active component of gamboge, exhibits potent anticancer activity in many kinds of cancer cells. However, the anticancer mechanism of GA is not clearly understood. Here we showed that GA could cause growth inhibition, induce the G0/G1 phase cell cycle arrest and apoptosis in human chronic my...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2009.04.059
更新日期:2009-08-03 00:00:00
abstract::The multiple cascades of signal transduction pathways that lead from receptors on the cell membrane to the nucleus, thus translating extracellular signals into changes in gene expression, may represent important targets for neurotoxic compounds. Among the biochemical steps and pathways that have been investigated are ...
journal_title:Toxicology
pub_type: 杂志文章,评审
doi:10.1016/s0300-483x(00)00435-2
更新日期:2001-03-07 00:00:00
abstract::Hesperidin (HDN) is a flavanone glycoside abundantly found in citrus fruits. HDN has been reported to possess significant activities against allergy, haemorrhoids, hormonal disorders and ulcers. Other reported activities include anti-inflammatory, analgesic, antibacterial, antifungal, antiviral, antioxidant and free r...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2006.06.018
更新日期:2006-09-21 00:00:00
abstract::We investigate the ability of S-adenosylmethionine (SAMe) to antagonize the cyclosporine A (CyA)-induced inhibition of biliary glutathione efflux induced by long-term administration of CyA (10 mg/kg per day-CyA(10) or 20 mg/kg per day-CyA(20) for 4 weeks) in rats. CyA treatment reduced the liver content of total gluta...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2004.02.030
更新日期:2004-07-15 00:00:00
abstract::Previous in vivo studies in rats have shown that susceptibility to 1,3-dinitrobenzene (DNB)-induced testicular damage increases with age. The present study has used an in vitro approach to investigate the possibility that differences in testicular metabolism contribute to the age-related differences in toxicity. Semin...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(97)00111-x
更新日期:1997-11-21 00:00:00
abstract::p-Dichlorobenzene (p-DCB) is widely used as a moth repellent and a space deodorant. In mice pretreated with DL-buthionine sulfoximine (BSO; 2 mmol/kg or higher doses, i.p.), an inhibitor of glutathione (GSH) synthesis, administration of p-DCB (100-400 mg/kg, p.o.) resulted in dose-dependent hepatotoxicity as judged by...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(94)90028-0
更新日期:1994-11-01 00:00:00
abstract::Exposure to polychlorinated biphenyls (PCBs) is associated with numerous adverse health effects. Although the main route of exposure to PCBs is through the gastrointestinal tract, little is known about the contribution of the gut to the health effects of PCBs. We hypothesize that PCBs can disrupt intestinal integrity,...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2012.08.001
更新日期:2012-12-16 00:00:00
abstract::Simple and efficient ex vivo/in vitro screening systems for contact allergens are developed for alternative to conventional animal tests. We have previously proposed an ex vivo/in vitro proliferation assay as a first stage screening method with advantages over existing alternatives, using lymph node cells (LNC) from s...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(96)89846-5
更新日期:1996-11-15 00:00:00
abstract::The induction of unscheduled DNA synthesis (UDS) in early spermatid stages of mice was studied after treatment with hydrazine or procarbazine (Natulan). The UDS response was assayed by measuring the levels of [3H]dThd in the nuclei of sperm cells derived from treated and untreated early spermatid stages. The radioacti...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(82)90030-0
更新日期:1982-01-01 00:00:00
abstract::The effects of N-acetylcysteine (NAC), 2-mercaptopropionylglycine (MPG) and dithiothreitol (DTT) on the metabolism and toxicity of acetaminophen (APAP) were examined in isolated mouse hepatocytes maintained in primary culture on collagen-coated dishes. Both NAC and MPG increased the formation of the glutathione and su...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(86)90106-x
更新日期:1986-10-01 00:00:00
abstract::Aconitine (ACO) is a highly toxic diterpenoid alkaloid and known to exert the immunomodulatory action. However, whether it has any effects on ion currents in immune cells remains unknown. The effects of ACO and other related compounds on ion currents in Jurkat T-lymphocytes were investigated in this study. ACO suppres...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2011.07.003
更新日期:2011-10-28 00:00:00
abstract::Silicosis is a universal occupational disease, which is caused by long-term crystalline silica exposure. Recent studies have shown that noncoding RNAs participate in diverse pathological cellular pathways. However, the precise regulation mechanism remains limited in silicosis. Here, we established a silica-induced mou...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2021.152683
更新日期:2021-01-19 00:00:00
abstract::The development of poisons information centers has in general complied with the actual needs and conditions in the society, and a pragmatic approach and limited funds have been characteristic for these units. To operate in a satisfying way a poisons information center is dependent on two cornerstones-a well educated a...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2004.01.033
更新日期:2004-05-20 00:00:00
abstract::Static and rotary cultures of adult rabbit hepatocytes can be produced reproducibly following their isolation by means of a simplified, inexpensive recycling perfusion system. Light and electron microscopic examination of the cultures showed that these hepatocytes retained the morphological characteristics of fully di...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(82)90052-x
更新日期:1982-01-01 00:00:00
abstract::The objective of this study was to observe whether 1.8 GHz microwaves (MW) (SAR, 3 W/kg) exposure can influence human lymphocyte DNA damage induced by ultraviolet ray C (UVC). The lymphocytes, which were from three young healthy donors, were exposed to 254 nm UVC at the doses of 0.25, 0.5, 0.75, 1.0, 1.5 and 2.0 J m(-...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2007.01.019
更新日期:2007-04-11 00:00:00
abstract::The Salmonella mutagenicity assay was utilized to compare hepatic S9 fractions derived from wild and laboratory reared woodchucks (Marmota monax). Two promutagens, 7,12-dimethylbenz[a]anthracence (DMBA) and 2-amino-fluorene (AF) were tested at 5 concentrations with the tester strains TA98 and TA100, against 2 levels o...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(88)90058-3
更新日期:1988-01-01 00:00:00
abstract::The mouse lymphoma thymidine kinase assay (MLA) has been optimized to quantitatively determine the in vitro mutagenicity of cigarette mainstream smoke particulate phase. To test whether the MLA is able to discriminate between different cigarette types, specially constructed cigarettes each containing a single tobacco ...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2006.07.019
更新日期:2006-10-29 00:00:00
abstract::Challenges to improve toxicological risk assessment to meet the demands of the EU chemical's legislation, REACH, and the EU 7th Amendment of the Cosmetics Directive have accelerated the development of non-animal based methods. Unfortunately, uncertainties remain surrounding the power of alternative methods such as in ...
journal_title:Toxicology
pub_type: 杂志文章,评审
doi:10.1016/j.tox.2013.08.012
更新日期:2015-06-05 00:00:00