Abstract:
:In order to find the immune-relevant factors responsible for the virus resistance in the WSSV-resistant shrimp, a suppression subtractive hybridization method was employed to identify differentially expressed genes and their expression profile in the hepatopancreas of the virus-resistant penaeid shrimp. Thirty five genes were identified from more than 400 clones, of which eight are found for the first time in penaeid shrimp. betaGBP is the most abundant gene in our subtractive library except hemocyanin. Lectin, ferritin, oxygenase and chitinase of the virus-resistant shrimp all showed up-regulation in expression compared with those of normal shrimp. In addition, Ranbp, Rho and Rab were found in the subtractive library. This is the first evidence indicating that small GTPases are involved in the signal transduction in shrimp defense response. Furthermore, a number of genes encoding apoptotic-related proteins and antioxidant enzymes were expressed at a higher level in the virus-resistant shrimp. In short, the high expression of immuno-related genes in response to the virus infection and the involvement of small GTPases in the antiviral response provide a new insight for further study in the crustacean innate immunity.
journal_name
Dev Comp Immunoljournal_title
Developmental and comparative immunologyauthors
Pan D,He N,Yang Z,Liu H,Xu Xdoi
10.1016/j.dci.2004.07.001keywords:
subject
Has Abstractpub_date
2005-01-01 00:00:00pages
103-12issue
2eissn
0145-305Xissn
1879-0089pii
S0145-305X(04)00105-3journal_volume
29pub_type
杂志文章abstract::Levels of interferon (IFN)-γ and interleukin (IL)-10 were measured in the serum of immunocompetent and immunosuppressed New Zealand White rabbits naturally infected with Encephalitozoon cuniculi. IFN-γ levels were elevated in infected rabbits, and a synergic effect was observed in animals treated with the immunosuppre...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2016.05.003
更新日期:2016-09-01 00:00:00
abstract::Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases mainly involved in extracellular matrix (ECM) degradation. We have cloned and identified BbMMPL2 as homolog of MMPs from adult amphioxus. Recombinant BbMMPL2 proteins underwent self-processing during refolding in vitro. The final ~23 kDa po...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2012.02.015
更新日期:2012-07-01 00:00:00
abstract::Marijuana components modulate a variety of immune response parameters. The cannabinoids delta 9-tetrahydrocannabinol (THC) and 11-hydroxy-tetrahydrocannabinol (11 OH-THC) are known to depress the in vitro proliferative response of murine lymphoid cells to the mitogens concanavalin A (Con A) and phytohemagglutinin (PHA...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/0145-305x(90)90015-7
更新日期:1990-01-01 00:00:00
abstract::Thymic non-lymphoid cells have been shown to influence the differentiation of T-lymphocytes. Large numbers of non-lymphoid cells are concentrated in the thymic medullary zone. A morphometric analysis of these cells in the medulla identified little change in their absolute number and volume density during thymic develo...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/0145-305x(84)90102-2
更新日期:1984-07-01 00:00:00
abstract::The αβ T cells are important components of the adaptive immune system and can recognize a vast array of peptides presented by MHC molecules. The ability of these T cells to recognize the complex depends on the diversity of the αβ TR, which is generated by a recombination of specific Variable, Diversity and Joining gen...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2017.05.021
更新日期:2017-11-01 00:00:00
abstract::Immunofluorescence studies of bream lymphoid tissues revealed that our 90% of the lymphocytes from blood, anterior kidney, spleen and thymus exhibited membrane immunoglobulin determinants. Furthermore, a majority of such cells were observed to undergo patching and capping when the membrane proteins were complexed with...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/s0145-305x(77)80004-9
更新日期:1977-04-01 00:00:00
abstract::Iron functions as a nutrient and a potential toxin in all organisms. It plays a key role in the interaction between microbes and their hosts as well. Microbial infection disrupts iron homeostasis in the host; meanwhile the host endeavors to keep the homeostasis through iron transport and storage. Transferrins and ferr...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2015.10.022
更新日期:2016-02-01 00:00:00
abstract::Yellow head virus (YHV) is one of the most widespread viruses seriously affecting black tiger shrimp (Penaeus monodon) cultivation. A previous microarray study demonstrated that clathrin coat assembly protein 17 (AP17) was significantly up-regulated after YHV infection (Pongsomboon et al., 2011). Clathrin coat AP17 is...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2013.11.017
更新日期:2014-05-01 00:00:00
abstract::Fusion experiments with three colonies (AC-BC-BD) by Tanaka and Watanabe indicated that the mixture of blood from two nonfusible colonies induced a "nonfusion" reaction (NFR)-like responses. Thus, artificial mixture of the blood from two nonfusible colonies is expected to result in NFR-like reactions. The present stud...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/s0145-305x(82)80007-4
更新日期:1982-04-01 00:00:00
abstract::Next generation sequencing (NGS) allows for the rapid, comprehensive and cost effective analysis of entire genomes and transcriptomes. NGS provides approaches for immune response gene discovery, profiling gene expression over the course of parasitosis, studying mechanisms of diversification of immune receptors and inv...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章,评审
doi:10.1016/j.dci.2014.01.022
更新日期:2014-07-01 00:00:00
abstract::Marek's disease (MD) vaccines, although effective in reducing lymphoproliferation, cannot control infectious virus production in the feather follicle epithelium (FFE) which is the site of virus shedding. Therefore, we investigated Marek's disease virus (MDV) replication as well as the expression of cytokine genes in f...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2008.11.003
更新日期:2009-04-01 00:00:00
abstract::Temperature variability, and in particular temperature decreases, can increase susceptibility of amphibians to infections by the fungus Batrachochytrium dendrobatidis (Bd). However, the effects of temperature shifts on the immune systems of Bd-infected amphibians are unresolved. We acclimated frogs to 16 °C and 26 °C ...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2017.08.018
更新日期:2017-12-01 00:00:00
abstract::Interferon regulatory factors (IRFs) are transcription factors that play important roles in immune defense, stress response, hematopoietic differentiation, and cell apoptosis. IRFs of invertebrate organisms and their functions remain largely unexplored. In the present study, for the first time new IRFs (BpIRF2 and MmI...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2017.01.015
更新日期:2017-06-01 00:00:00
abstract::The Ikaros gene encodes a family of transcription factors which plays a crucial role in hematopoiesis. To improve our knowledge about the immune system of Pleurodeles waltl, we sequenced the cDNA coding for the Ik-1 isoform of that salamander and analyzed its tissue expression by semi-quantitative RT-PCR. Ikaros trans...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/s0145-305x(02)00014-9
更新日期:2002-09-01 00:00:00
abstract::To date, many immunoglobulin (Ig) genes have been identified in diverse teleost species, but the contributions of different types of light chain (IgL) to the immune response remain unclear. Screening of a stimulated kidney cDNA library from orange-spotted grouper (Osg, Epinephelus coioides) resulted in the identificat...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2012.11.003
更新日期:2013-03-01 00:00:00
abstract::The development of mammalian megakaryocytes and platelets is regulated by numerous cytokine signals, primarily through the thrombopoietin (TPO)/c-MPL axis. Although non-mammalian vertebrates are known to possess nucleated thrombocytes functionally equivalent to mammalian platelets, the dynamics of the thrombocyte deve...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2018.03.005
更新日期:2018-07-01 00:00:00
abstract::Immunoglobulin heavy chain (IgH) variable (V) region cDNAs from the Atlantic salmon, Salmo salar L., have been isolated and analysed with respect to diversity and transcription of the two parallel IgH isoloci in this species. A total of nine V(H) families were defined according to the 80% identity criterion, of which ...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/s0145-305x(01)00008-8
更新日期:2001-06-01 00:00:00
abstract::The appearance of antibody genes over evolution coincided with the origin of the vertebrates. Reptiles are of great interest in evolution since they are the link between the amphibians, birds, and mammals. This work describes the presence of a gene in the reptile leopard gecko (Eublepharis macularius) where phylogenet...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2006.09.005
更新日期:2007-01-01 00:00:00
abstract::The Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling pathway is one of the main pleiotropic cascades used to transmit information from extracellular receptors to the nucleus, which results in DNA transcription and expression of genes involved in immunity, proliferation, differentiat...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2017.12.019
更新日期:2018-04-01 00:00:00
abstract::Adult female frogs (Rana perezi) have been used to analyze structural and morphometrical changes which affect lymphoid organs after treatment with a single dose of dexamethasone (DX). Special attention was given to the distinct sensitivity observed during two different periods of the year. Morphological differences we...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/0145-305x(89)90017-7
更新日期:1989-01-01 00:00:00
abstract::Cathelicidins were initially characterized as a family of antimicrobial peptides. Now it is clear that they fulfill several immune functions in addition to their antimicrobial activity. In the current work, three cDNA sequences encoding pheasant cathelicidins were cloned from a constructed bone marrow cDNA library of ...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2010.10.004
更新日期:2011-03-01 00:00:00
abstract::Studies of the innate immune system have recently shown that, in addition to its role in producing the primary response that slows down pathogens, it may also play an important role in initiating and directing the type of response that the adaptive immune system makes. These discoveries have shown a complex web of con...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章,评审
doi:10.1016/s0145-305x(01)00030-1
更新日期:2001-10-01 00:00:00
abstract::A comprehensive analysis of cattle shotgun sequencing data reveals 36 immunoglobulin heavy chain variable genes. The previously described bovine subgroup IGHV1 contains 10 functional genes with a conserved promoter including the consensus octamer and several other transcription factor binding sites, intact exons and m...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2012.02.006
更新日期:2012-07-01 00:00:00
abstract::RNA interference (RNAi) is reported here for the first time for Biomphalaria glabrata, the snail intermediate host for the human parasite Schistosoma mansoni. The fibrinogen-related protein 2 (FREP2) gene, normally expressed at increased levels following exposure to digenetic trematode parasites, such as S. mansoni or...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2005.12.004
更新日期:2006-01-01 00:00:00
abstract::The identification of molecular markers considerably facilitated the classification and functional analysis of blood cell types. Apis mellifera hemocytes have been classified by morphological criteria and lectin binding properties; however, the use of molecular markers has been minimal. Here we describe a monoclonal a...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2017.07.013
更新日期:2017-11-01 00:00:00
abstract::Lymphocytes from tumor-bearing damselfish are cytotoxic towards target cell lines derived from damselfish neurofibromatosis. These cell lines contain at least one retrovirus which appears to be related to the etiology of the disease. The current studies were designed to characterize the effectors of this cytotoxic rea...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/s0145-305x(97)00010-4
更新日期:1997-05-01 00:00:00
abstract::Population declines due to chytridiomycosis among frogs belonging to the Amerana (Rana boylii) species group from western North America have been particularly severe. Norepinephrine-stimulated skin secretions from the Oregon spotted frog Rana pretiosa Baird and Girard, 1853 were collected from individuals that had bee...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2011.01.017
更新日期:2011-06-01 00:00:00
abstract::The insect phenoloxidase (PO) cascade is known to be tightly regulated by serine proteases and serine protease inhibitors of the serpin family. As a key component of the insect immune system, it is also suspected to be inhibited by several endoparasitoid wasps, insects that develop inside other arthropods as hosts. Ho...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2008.11.013
更新日期:2009-05-01 00:00:00
abstract::Damselfish neurofibromatosis (DNF) is a malignant transmissible disease affecting Schwann cells, and is the only naturally occurring animal model of human neurofibromatosis type 1. The current study was designed to determine whether fish in the early stages of disease have measurable immune responses toward DNF tumor ...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/s0145-305x(94)90356-5
更新日期:1994-07-01 00:00:00
abstract::In a previous report we described the existence of two structural and antigenic variants of light chains in serum trout Igs, defined by mAbs 2H9 and 2A1. In this report, we analyse the cell distribution, the "in vitro" kinetics of production, and the ontogenic appearance of these two variants. Both variants were alrea...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/0145-305x(94)00058-n
更新日期:1995-01-01 00:00:00