Agitation rate effects on plasmid stability in immobilized and free-cell continuous cultures of recombinant E. coli.

abstract：:Controlled hydrolysis of lactonic sophorolipids from Starmerella bombicola yields a previously undescribed sophorose analog that potently induces cellulase in Trichoderma reesei Rut-C30. Acid treatment of natural sophorolipids results in a mixture of monoacetylated, deacetylated, and diacetylated sophorolipids in acid...

journal_title：Enzyme and microbial technology

authors： Huang TT,Wages JM

更新日期：2016-04-01 00:00:00

abstract：:Effects of different microaerophilic conditions on cell growth, glucose consumption, hydrogen production and cellular metabolism of wild Enterobacter aerogenes strain and polyphosphate kinase (PPK) overexpressing strain were systematically studied in this paper, using NaH(2)PO(4) as the phosphate sources. Under differ...

journal_title：Enzyme and microbial technology

authors： Lu Y,Zhang C,Lai Q,Zhao H,Xing XH

更新日期：2011-02-08 00:00:00

abstract：:The cellulase and xylanase genes of filamentous Trichoderma fungi exist under carbon catabolite repression mediated by the regulator carbon catabolite repressor (CREI). Our objective was to find the role of CREI in a cellulase-hyperproducing mutant of Trichoderma koningii, and address whether enzyme production can be ...

journal_title：Enzyme and microbial technology

authors： Wang S,Liu G,Yu J,Tian S,Huang B,Xing M

更新日期：2013-07-10 00:00:00

abstract：:Two acidic β-glucosidases (βGI and βGII) from the brown rot fungus Fomitopsis palustris were purified to homogeneity by several chromatographic steps. βGI and βGII had molecular weights of 130 and 213 kDa, respectively, and exhibited optimum activity at pH 2.5 and 55°C. The K(m) values of βGI and βGII for p-nitropheny...

journal_title：Enzyme and microbial technology

authors： Okamoto K,Sugita Y,Nishikori N,Nitta Y,Yanase H

更新日期：2011-04-07 00:00:00

abstract：:The mechanical properties of TB/C3 hybridoma cells taken from a continuous culture were measured by micromanipulation. The culture conditions were constant except for the presence or absence of Pluronic F-68 in the medium. It was found that the mean bursting membrane tension and the mean elastic area compressibility m...

journal_title：Enzyme and microbial technology

authors： Zhang Z,al-Rubeai M,Thomas CR

更新日期：1992-12-01 00:00:00

abstract：:A colorimetric platform based on enzyme-inorganic hybrid nanoflowers was used for ultraviolet-visible detection of hydrogen peroxide (H2O2). The hybrid nanoflowers were prepared via incubation at room temperature using horseradish peroxidase (HRP) and copper phosphate (Cu3(PO4)2) as the organic and inorganic component...

journal_title：Enzyme and microbial technology

authors： Yang C,Zhang M,Wang W,Wang Y,Tang J

更新日期：2020-10-01 00:00:00

abstract：:Oxygen uptake rate and oxygen mass transfer rate have been studied during xanthan gum production process in stirred tank bioreactor. Empirical equations for the oxygen mass transfer coefficient have been obtained taking into account several variables such as air flow rate, stirrer speed and apparent viscosity. Oxygen ...

journal_title：Enzyme and microbial technology

authors： García-Ochoa F,Castro EG,Santos VE

更新日期：2000-11-15 00:00:00

abstract：:Three threonine aldolases (TAs) were cloned and overexpressed in Escherichia coli (Aeromonas jandaeil-allo-threonine aldolase, E. colil-threonine aldolase and Thermotoga maritimal-allo-threonine aldolase). A Design of Experiments strategy was used to identify optimal reaction conditions for each enzyme. These conditio...

journal_title：Enzyme and microbial technology

authors： Beaudoin SF,Hanna MP,Ghiviriga I,Stewart JD

更新日期：2018-12-01 00:00:00

abstract：:Bacterial chitinases recruited multiple accessory domains for the conversion of recalcitrant polysaccharides to simple soluble sugars/amino sugars. Here, we report detailed properties of a multi-domain GH18 chitinase from Enterobacter cloacae subsp. cloacae (EcChi2) that preferred β-chitin as substrate. EcChi2 exhibit...

journal_title：Enzyme and microbial technology

authors： Mallakuntla MK,Podile AR

更新日期：2021-02-01 00:00:00

abstract：:Photothermal therapy (PTT) is a minimally invasive and effective cancer treatment method and has a great potential for innovating the conventional chemotherapy approaches. Copper sulfide (CuS) exhibits photostability, low cost, and high absorption in near infrared region, and is recognized as an ideal candidate for PT...

journal_title：Enzyme and microbial technology

authors： Zhou NQ,Tian LJ,Wang YC,Li DB,Li PP,Zhang X,Yu HQ

更新日期：2016-12-01 00:00:00

abstract：:Addition of non-ionic surfactants (NIS) is known to accelerate enzymatic lignocellulose hydrolysis. The mechanism behind this accelerating effect is still not elucidated but has been hypothesized to originate from favorable NIS-lignin interactions which alleviate non-productive adsorption of cellulases to lignin. In t...

journal_title：Enzyme and microbial technology

authors： Olsen SN,Bohlin C,Murphy L,Borch K,McFarland KC,Sweeny MD,Westh P

更新日期：2011-09-10 00:00:00

abstract：:Enzymatic production of cephalexin using immobilized penicillin G acylase was studied. The factors that affect the conversion of 7-amino-3-deacetoxycephalosporanic acid (7-ADCA) to cephalexin, such as pH; temperature; concentrations of 7-ADCA, D(-)-phenylglycine methyl ester (PGME), and immobilized penicillin G acylas...

journal_title：Enzyme and microbial technology

authors： Maladkar NK

更新日期：1994-08-01 00:00:00

abstract：:By using very active and very stable penicillin G acylase (PGA)--agarose derivatives we have studied the industrial design of equilibrium-controlled synthesis of lactamic antibiotics. In the presence of high concentrations of organic cosolvents we have carried out the direct enzymatic condensation of phenylacetic acid...

journal_title：Enzyme and microbial technology

authors： Fernandez-Lafuente R,Rosell CM,Guisán JM

更新日期：1991-11-01 00:00:00

abstract：:Nitrification kinetics of synthetic saline wastewater was investigated by using an activated sludge unit. Initial experiments were performed with salt-free wastewater to obtain baseline information. Experiments with 3% salt concentration were performed at different sludge ages in order to investigate the system perfor...

journal_title：Enzyme and microbial technology

authors： Dinçer AR,Kargi F

更新日期：2001-05-07 00:00:00

abstract：:An improved amperometric l-lactate biosensor was constructed based on covalent immobilization of lactate oxidase (LOx) from Pediococcus species onto carboxylated multiwalled carbon nanotubes (cMWCNT)/copper nanoparticles (CuNPs)/polyaniline (PANI) hybrid film electrodeposited on the surface of a pencil graphite electr...

journal_title：Enzyme and microbial technology

authors： Dagar K,Pundir CS

更新日期：2017-01-01 00:00:00

abstract：:In this study, distillery wastewater was treated by dark fermentation or photofermentation alone, and by sequential dark and photofermentation processes using anaerobic saccharolytic consortium and purple nonsulfur bacteria. Combination of dark and photofermentation resulted in the maximal H2 yield of 17.6L/L of disti...

journal_title：Enzyme and microbial technology

authors： Laurinavichene T,Tekucheva D,Laurinavichius K,Tsygankov A

更新日期：2018-03-01 00:00:00

abstract：:Astragalin (kaempferol-3-O-β-D-glucopyranoside, Ast) glucosides were synthesized by the acceptor reaction of a dextransucrase produced by Leuconostoc mesenteroides B-512FMCM with astragalin and sucrose. Each glucoside was purified and their structures were assigned as kaempferol-3-O-β-D-glucopyranosyl-(1→3)-O-α-D-gluc...

journal_title：Enzyme and microbial technology

authors： Kim GE,Kang HK,Seo ES,Jung SH,Park JS,Kim DH,Kim DW,Ahn SA,Sunwoo C,Kim D

更新日期：2012-01-05 00:00:00

abstract：:Human interferon alpha 2b (IFN α2b) is a type I interferon exhibiting antiviral, anti-proliferative and immunomodulatory activities. The clinical outcome of the approved recombinant human IFN α2b drugs in the market suffers from short plasma half-life, rapid clearance and other side effects. Human IFN α2b expression i...

journal_title：Enzyme and microbial technology

authors： Katla S,Yoganand KNR,Hingane S,Ranjith Kumar CT,Anand B,Sivaprakasam S

更新日期：2019-09-01 00:00:00

abstract：:Non-productive cellulase adsorption onto lignin is a major inhibitory mechanism preventing enzymatic hydrolysis of lignocellulosic feedstocks. Therefore, understanding of enzyme-lignin interactions is essential for the development of enzyme mixtures and processes for lignocellulose hydrolysis. We have studied cellulas...

journal_title：Enzyme and microbial technology

authors： Rahikainen JL,Evans JD,Mikander S,Kalliola A,Puranen T,Tamminen T,Marjamaa K,Kruus K

更新日期：2013-10-10 00:00:00

abstract：:An analysis developed by Westgate et al. for the digestible energy of edible and inedible biomass, including hydrolysis and fermentation, is reexamined with state-of-the-art values for the harvest index of hydroponic crops. ...

journal_title：Enzyme and microbial technology

authors： Volk T

更新日期：1993-10-01 00:00:00

abstract：:Turanose is a natural isomer of sucrose. It is an emerging functional sweetener of the next generation. Turanose is catalytically synthesized from the sucrose biomass by employing amylosucrase enzyme. In this study, a novel gene encoding amylosucrase (Asmet) has been identified from the metagenome of a thermal aquatic...

journal_title：Enzyme and microbial technology

authors： Agarwal N,Narnoliya LK,Singh SP

更新日期：2019-12-01 00:00:00

abstract：:Laccases (benzenediol oxygen oxidoreductases, EC 1.10.3.2) are important multicopper enzymes that are used in many biotechnological processes. A recombinant form of laccase from Bacillus sp. HR03 was overexpressed in Escherichia coli BL-21(DE3). Inclusion body (IB) formation happens quite often during recombinant prot...

journal_title：Enzyme and microbial technology

authors： Mollania N,Khajeh K,Ranjbar B,Rashno F,Akbari N,Fathi-Roudsari M

更新日期：2013-05-10 00:00:00

abstract：:Biosensors have been extensively studied during the last 20 years, and a myriad of laboratory biosensors have been developed. Improvements are required in biosensor design and performance before they become widely accepted in industrial process monitoring. However, as the biotechnology industry expands, biosensors may...

journal_title：Enzyme and microbial technology

authors： Brooks SL,Higgins IJ,Newman JD,Turner AP

更新日期：1991-12-01 00:00:00

abstract：:2,5-Bis(hydroxymethyl)furan (BHMF) is a versatile building block in the synthesis of polymers, fuels, and macrocycle polyethers. In this work, alcohol dehydrogenases (ADHs) were identified from Meyerozyma guilliermondii SC1103 and were heterologously expressed in Saccharomyces cerevisiae for the synthesis of BHMF from...

journal_title：Enzyme and microbial technology

authors： Xia ZH,Zong MH,Li N

更新日期：2020-03-01 00:00:00

abstract：:Glutaric acid is one of the promising C5 platform compounds in the biochemical industry. It can be produced chemically, through the ring-opening of butyrolactone followed by hydrolysis. Alternatively, glutaric acid can be produced via lysine degradation pathways by microorganisms. In microorganisms, the overexpression...

journal_title：Enzyme and microbial technology

authors： Hong YG,Moon YM,Hong JW,No SY,Choi TR,Jung HR,Yang SY,Bhatia SK,Ahn JO,Park KM,Yang YH

更新日期：2018-11-01 00:00:00

abstract：:Progesterone 5β-reductases (P5βRs) are involved in 5β-cardenolide formation by stereo-specific reduction of the △4,5 double bond of steroid precursors. In this study a steroid 5β-reductase was identified in Capsella rubella (CrSt5βR1) and its function in steroid 5β-reduction was validated experimentally. CrSt5βR1 is c...

journal_title：Enzyme and microbial technology

authors： Li Y,Pan H,Chang Y,Dong N,Zou L,Liang P,Tian W,Chang Z

更新日期：2020-03-01 00:00:00

abstract：:The heat and mass transfer have been proved to be the important factors in air pressure pulsation for cellulase production. However, as process of enzyme secretion, the cellulase formation has not been studied in the view of microorganism metabolism and metabolic key enzyme activity under air pressure pulsation condit...

journal_title：Enzyme and microbial technology

authors： Chen H,Shao M,Li H

更新日期：2014-03-05 00:00:00

abstract：:A three-phase fluidized bed reactor (TPFBR) was designed to evaluate the potential of CoQ(10) production by gel-entrapped Sphingomonas sp. ZUTE03 via a conversion-extract coupled process. In the reactor, the CoQ(10) yield reached 46.99 mg/L after 8 h of conversion; a high-level yield of about 45 mg/L was maintained ev...

journal_title：Enzyme and microbial technology

authors： Qiu L,Ding H,Wang W,Kong Z,Li X,Shi Y,Zhong W

更新日期：2012-02-10 00:00:00

abstract：:A novel endoglucanase active on 1,3-1,4-beta-D-glucans was purified to apparent homogeneity from submerged cultures of the moderately thermophilic aerobic fungus Talaromyces emersonii CBS 814.70. The enzyme is a single subunit glycoprotein with M(r) and pI values of 40.7 +/- 0.3 kDa and 4.4, respectively, and an estim...

journal_title：Enzyme and microbial technology

authors： Murray PG,Grassick A,Laffey CD,Cuffe MM,Higgins T,Savage AV,Planas A,Tuohy MG

更新日期：2001-07-05 00:00:00

abstract：:Ergosterol, like cholesterol, has many beneficial physiological activities, and because it has no adverse clinical problem with cholesterol, it is a substance that can be used as a biomaterial in the food and cosmetics industry. However, ergosterol has low oil solubility and is easily crystallized, which is problemati...

journal_title：Enzyme and microbial technology

authors： Park SH,Kim HK

更新日期：2020-09-01 00:00:00