Abstract:
:The cellulase and xylanase genes of filamentous Trichoderma fungi exist under carbon catabolite repression mediated by the regulator carbon catabolite repressor (CREI). Our objective was to find the role of CREI in a cellulase-hyperproducing mutant of Trichoderma koningii, and address whether enzyme production can be further improved by silencing the cre1 gene. cre1 partially silenced strains were constructed to improve enzyme production in T. koningii YC01, a cellulase-hyperproducing mutant. Silencing of cre1 resulted in derepression of cellulase gene expression in glucose-based cultivation. The cre1 interference strain C313 produced 2.1-, 1.4-, 0.8-, and 0.8-fold higher amounts of filter paper activity, β-1,4-exoglucanase activity (ρ-nitrophenyl-β-D-cellobioside as substrate), β-1,4-endoglucanase activity (sodium carboxymethyl cellulose as substrate), and xylanase activity, respectively, than the control strain, suggesting that silencing of cre1 resulted in enhanced enzyme production capability. In addition, downregulation of cre1 resulted in elevated expression of another regulator of xylanase and cellulase expression, xyr1, indicating that CREI also acted as a repressor of xyr1 transcription in T. koningii under inducing conditions. These results show that RNAi is a feasible method for analyzing the regulatory mechanisms of gene expression and improving xylanase and cellulase productivity in T. koningii.
journal_name
Enzyme Microb Technoljournal_title
Enzyme and microbial technologyauthors
Wang S,Liu G,Yu J,Tian S,Huang B,Xing Mdoi
10.1016/j.enzmictec.2013.04.007subject
Has Abstractpub_date
2013-07-10 00:00:00pages
104-9issue
2eissn
0141-0229issn
1879-0909pii
S0141-0229(13)00097-5journal_volume
53pub_type
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journal_title:Enzyme and microbial technology
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journal_title:Enzyme and microbial technology
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pub_type: 杂志文章
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journal_title:Enzyme and microbial technology
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journal_title:Enzyme and microbial technology
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journal_title:Enzyme and microbial technology
pub_type: 杂志文章
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更新日期:2013-04-10 00:00:00