Abstract:
:The heat and mass transfer have been proved to be the important factors in air pressure pulsation for cellulase production. However, as process of enzyme secretion, the cellulase formation has not been studied in the view of microorganism metabolism and metabolic key enzyme activity under air pressure pulsation condition. Two fermentation methods in ATPase activity, cellulase productivity, weight lose rate and membrane permeability were systematically compared. Results indicated that gas double-dynamic solid state fermentation had no obviously effect on cell membrane permeability. However, the relation between ATPase activity and weight loss rate was linearly dependent with r=0.9784. Meanwhile, the results also implied that gas periodic stimulation had apparently strengthened microbial metabolism through increasing ATPase activity during gas double-dynamic solid state fermentation, resulting in motivating the production of cellulase by Trichoderma reesei YG3. Therefore, the increase of ATPase activity would be another crucial factor to strengthen fermentation process for cellulase production under gas double-dynamic solid state fermentation.
journal_name
Enzyme Microb Technoljournal_title
Enzyme and microbial technologyauthors
Chen H,Shao M,Li Hdoi
10.1016/j.enzmictec.2013.12.015subject
Has Abstractpub_date
2014-03-05 00:00:00pages
35-9eissn
0141-0229issn
1879-0909pii
S0141-0229(13)00261-5journal_volume
56pub_type
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