abstract：:Bacterial chitinases recruited multiple accessory domains for the conversion of recalcitrant polysaccharides to simple soluble sugars/amino sugars. Here, we report detailed properties of a multi-domain GH18 chitinase from Enterobacter cloacae subsp. cloacae (EcChi2) that preferred β-chitin as substrate. EcChi2 exhibit...

journal_title：Enzyme and microbial technology

authors： Mallakuntla MK,Podile AR

更新日期：2021-02-01 00:00:00

abstract：:Operational stability under high temperature is required for enzyme application in industrial processes. Error-prone PCR and B-factor analysis were employed to enhance the thermostability of a xylanase from GH family 11 in this study. Based on the top 10 mutants screened from the random mutation libraries, mutant Xyn3...

journal_title：Enzyme and microbial technology

authors： Xing H,Zou G,Liu C,Chai S,Yan X,Li X,Liu R,Yang Y,Zhou Z

更新日期：2021-02-01 00:00:00

abstract：:Bacterial spores maintain metabolic dormancy and have high resistance to external pressure. Germination requires degradation of the spore cortex and the participation of germination-specific cortex-lytic enzymes (GSLEs). Previously reported GSLEs have been identified in bacteria and facilitate germination. In this stu...

journal_title：Enzyme and microbial technology

authors： Fu Y,Liang L,Deng S,Wu Y,Yuan Y,Gao M

更新日期：2020-12-01 00:00:00

abstract：:'Candidatus Liberibacter asiaticus' ('Ca. L. asiaticus'), the suspected causative agent of citrus greening disease, is one of many phloem-restricted plant pathogens that have not been isolated and grown in an axenic culture. In this study, infected Asian citrus psyllids were used to prepare a host-free source of 'Ca. ...

journal_title：Enzyme and microbial technology

authors： Molki B,Call DR,Ha PT,Omsland A,Gang DR,Lindemann SR,Killiny N,Beyenal H

更新日期：2020-12-01 00:00:00

abstract：:Corynebacterium glutamicum is an important industrial organism for the production of a variety of biological commodities. We discovered a promoter encoded by the gene NCgl2319 in C. glutamicum, which could be induced by benzyl alcohol, could be used as an efficient tunable expression system. In initial attempts, this ...

journal_title：Enzyme and microbial technology

authors： Liu X,Zhao Z,Dong G,Li Y,Peng F,Liu C,Zhang F,Linhardt RJ,Yang Y,Bai Z

更新日期：2020-11-01 00:00:00

abstract：:6-(N-hydroxyethyl)-amino-6-deoxy-l-sorbofuranose (6NSL), a key precursor in the synthesis of miglitol, is produced from N-2-hydroxyethyl-glucamine (NHEG) by the regioselective oxidation of Gluconobacter oxydans. The limitation of PQQ biosynthesis became a bottleneck for improvement of PQQ-dependent D-sorbitol dehydrog...

journal_title：Enzyme and microbial technology

authors： Liu D,Ke X,Hu ZC,Zheng YG

更新日期：2020-11-01 00:00:00

abstract：:A colorimetric platform based on enzyme-inorganic hybrid nanoflowers was used for ultraviolet-visible detection of hydrogen peroxide (H2O2). The hybrid nanoflowers were prepared via incubation at room temperature using horseradish peroxidase (HRP) and copper phosphate (Cu3(PO4)2) as the organic and inorganic component...

journal_title：Enzyme and microbial technology

authors： Yang C,Zhang M,Wang W,Wang Y,Tang J

更新日期：2020-10-01 00:00:00

abstract：:Crocin, one of the major carotenoid pigments of Crocus sativus (saffron), is responsible for antioxidant activity, neuroprotection, and the inhibition of tumor cell proliferation. In order to improve the functionality of crocin, α-glucosyl-(1→6)-trans-crocins (C-Gs) were synthesized using sucrose and dextransucrase fr...

journal_title：Enzyme and microbial technology

authors： Mok IK,Nguyen TTH,Kim DH,Lee JW,Lim S,Jung HY,Lim T,Pal K,Kim D

更新日期：2020-10-01 00:00:00

abstract：:Long-chain alkyl glucosides, such as octyl and decyl β-d-glucopyranosides (OG and DG, respectively), are regarded as a new generation of biodegradable, non-ionic surfactants. Previously, the mutants of Dalbergia cochinchinensis Pierre dalcochinase showed potential in the synthesis of oligosaccharides and alkyl glucosi...

journal_title：Enzyme and microbial technology

authors： Thenchartanan P,Pitchayatanakorn P,Wattana-Amorn P,Ardá A,Svasti J,Jiménez-Barbero J,Kongsaeree PT

更新日期：2020-10-01 00:00:00

abstract：:Ergosterol, like cholesterol, has many beneficial physiological activities, and because it has no adverse clinical problem with cholesterol, it is a substance that can be used as a biomaterial in the food and cosmetics industry. However, ergosterol has low oil solubility and is easily crystallized, which is problemati...

journal_title：Enzyme and microbial technology

authors： Park SH,Kim HK

更新日期：2020-09-01 00:00:00

abstract：:Epoxide hydrolases (EHs) catalyse the conversion of epoxides into vicinal diols. These enzymes have extensive value in biocatalysis as they can generate enantiopure epoxides and diols which are important and versatile synthetic intermediates for the fine chemical and pharmaceutical industries. Despite these benefits, ...

journal_title：Enzyme and microbial technology

authors： Stojanovski G,Dobrijevic D,Hailes HC,Ward JM

更新日期：2020-09-01 00:00:00

abstract：:In this work, porous biochar derived from sugarcane bagasse was prepared and then coated with different amounts of chitosan (C@CS) for cellulase immobilization. Cellulase was covalently immobilized on the support by using glutaraldehyde as a linker. The chemical characteristics and morphology of the samples were deter...

journal_title：Enzyme and microbial technology

authors： Mo H,Qiu J,Yang C,Zang L,Sakai E,Chen J

更新日期：2020-08-01 00:00:00

abstract：:Phytate is the major storage form of phosphorus in plants. It is present in cereals and raw materials of vegetable origin used in animal and human diets. However, non-ruminant animals have little phytase activity in their guts and, therefore, cannot digest phytate. As a result, almost all dietary phytate is discharged...

journal_title：Enzyme and microbial technology

authors： Xie Z,Fong WP,Tsang PW

更新日期：2020-06-01 00:00:00

abstract：:Trehalose diesters are Gemini-type surfactants that might have better surface activity than conventional surfactants. A one-step method for the preparation of trehalose unsaturated fatty acid diesters has been successfully developed. The yield of trehalose diester of different unsaturated fatty acids was between 78 % ...

journal_title：Enzyme and microbial technology

authors： Ji S,Jia C,Cao D,Li S,Zhang X

更新日期：2020-05-01 00:00:00

abstract：:Amylosucrase (ASase) has great industrial potential owing to its multifunctional activities, including transglucosylation, polymerization, and isomerization. In the present study, the properties of Deinococcus geothermalis ASase (DGAS) expressed in Corynebacterium glutamicum (cDGAS) and purified via Ni-NTA affinity ch...

journal_title：Enzyme and microbial technology

authors： Chin YW,Jang SW,Shin HS,Kim TW,Kim SK,Park CS,Seo DH

更新日期：2020-04-01 00:00:00

abstract：:2,5-Bis(hydroxymethyl)furan (BHMF) is a versatile building block in the synthesis of polymers, fuels, and macrocycle polyethers. In this work, alcohol dehydrogenases (ADHs) were identified from Meyerozyma guilliermondii SC1103 and were heterologously expressed in Saccharomyces cerevisiae for the synthesis of BHMF from...

journal_title：Enzyme and microbial technology

authors： Xia ZH,Zong MH,Li N

更新日期：2020-03-01 00:00:00

abstract：:Progesterone 5β-reductases (P5βRs) are involved in 5β-cardenolide formation by stereo-specific reduction of the △4,5 double bond of steroid precursors. In this study a steroid 5β-reductase was identified in Capsella rubella (CrSt5βR1) and its function in steroid 5β-reduction was validated experimentally. CrSt5βR1 is c...

journal_title：Enzyme and microbial technology

authors： Li Y,Pan H,Chang Y,Dong N,Zou L,Liang P,Tian W,Chang Z

更新日期：2020-03-01 00:00:00

abstract：:To increase the efficiency of enzyme cocktails in deconstructing cellulose and hemicelluloses present in the plant cell wall, a combination of enzymes with complementary activities is required. Xylan is the main hemicellulose component of energy crops and for its complete hydrolysis a system consisting of several enzy...

journal_title：Enzyme and microbial technology

authors： Cintra LC,da Costa IC,de Oliveira ICM,Fernandes AG,Faria SP,Jesuíno RSA,Ravanal MC,Eyzaguirre J,Ramos LP,de Faria FP,Ulhoa CJ

更新日期：2020-02-01 00:00:00

abstract：:The present study investigated the influence of low-shear modeled microgravity (LSMMG) conditions on Listeria monocytogenes stress response (heat, cold, and acid), membrane fatty acid composition, and virulence potential as well as stress-/virulence-associated gene expression. The results showed that LSMMG-cultivated ...

journal_title：Enzyme and microbial technology

authors： Sheet S,Yesupatham S,Ghosh K,Choi MS,Shim KS,Lee YS

更新日期：2020-02-01 00:00:00

abstract：:Microorganism selection is critical to deglycosylation in soybean fermentation for producing beneficial phytochemicals. This study investigated isoflavone bioconversion in soybean extract inoculated with Lactobacillus plantarum K2-12 and Lactobacillus curvatus JD0-31 exhibiting different enzyme activities. L. plantaru...

journal_title：Enzyme and microbial technology

authors： Lim YJ,Lim B,Kim HY,Kwon SJ,Eom SH

更新日期：2020-01-01 00:00:00

abstract：:This work describes for the first time the green synthesis of neopentyl glycol diheptanoate in a solvent-free medium via an enzymatic pathway. The process has been carried out in an open-air reactor in order to ease water removal through evaporation and shift the chemical equilibrium towards product formation. The inh...

journal_title：Enzyme and microbial technology

authors： Serrano-Arnaldos M,García-Martínez JJ,Ortega-Requena S,Bastida J,Máximo F,Montiel MC

更新日期：2020-01-01 00:00:00

abstract：:In our previous study, we produced α-keto acids by using an L-amino acid deaminase PmiLAAD (wide-type) from Proteus mirabilis, however, the catalytic efficiency was low due to its low substrate affinity. In this study, protein engineering of PmiLAAD was performed to improve the α-keto acid production. PmiLAAD was engi...

journal_title：Enzyme and microbial technology

authors： Wu L,Guo X,Wu G,Liu P,Liu Z

更新日期：2020-01-01 00:00:00

abstract：:Turanose is a natural isomer of sucrose. It is an emerging functional sweetener of the next generation. Turanose is catalytically synthesized from the sucrose biomass by employing amylosucrase enzyme. In this study, a novel gene encoding amylosucrase (Asmet) has been identified from the metagenome of a thermal aquatic...

journal_title：Enzyme and microbial technology

authors： Agarwal N,Narnoliya LK,Singh SP

更新日期：2019-12-01 00:00:00

abstract：:The hydrolytic activity of a thermophilic cyclomaltodextrinase (CMD) from Anoxybacillus flavithermus ZNU-NGA and a representative single mutant were investigated against soluble substrates including α-, β- and γ-cyclomaltodestrines (CDs). Based on the occurrence of arginine (Arg) at position 403 in some homologue prot...

journal_title：Enzyme and microbial technology

authors： Mirzaee Z,Jafarian V,Shirdel SA,Khalifeh K

更新日期：2019-12-01 00:00:00

abstract：:Musky macrolactones are an important group of compounds used in high-valued perfumery. An enzymatic cascade reaction including cytochrome P450 hydroxylase and lipase was explored to biosynthesize musky macrolactones. Firstly, fatty acids were hydroxylated by P450 hydroxylase to produce the corresponding ω-hydroxy fatt...

journal_title：Enzyme and microbial technology

authors： Meng S,Guo J,Li Z,Nie K,Xu H,Tan T,Liu L

更新日期：2019-12-01 00:00:00

abstract：:GH11 xylanase XynJ from Bacillus sp. strain 41M-1 has a β-jellyroll fold composed of eight β strands with a deep active-site cleft. We hypothesized that the thermostability of XynJ will increase if the flexibility of the β strands in the jellyroll structure is decreased without impairing activity. To verify this hypot...

journal_title：Enzyme and microbial technology

authors： Takita T,Nakatani K,Katano Y,Suzuki M,Kojima K,Saka N,Mikami B,Yatsunami R,Nakamura S,Yasukawa K

更新日期：2019-11-01 00:00:00

abstract：:Kumamolisin from Alicyclobacillus sendaiensis strain NTAP-1 is a serine protease with collagenase activity. After molecular engineering, a kumamolisin mutant, named Kuma030, was obtained with high proteolytic activity against gluten, which might cause celiac disease. Kuma030 exhibited its potential application in indu...

journal_title：Enzyme and microbial technology

authors： Liu H,Fan X,Song H,Hu X,Zhang G,Yu C,Yi L

更新日期：2019-10-01 00:00:00

abstract：:Human interferon alpha 2b (IFN α2b) is a type I interferon exhibiting antiviral, anti-proliferative and immunomodulatory activities. The clinical outcome of the approved recombinant human IFN α2b drugs in the market suffers from short plasma half-life, rapid clearance and other side effects. Human IFN α2b expression i...

journal_title：Enzyme and microbial technology

authors： Katla S,Yoganand KNR,Hingane S,Ranjith Kumar CT,Anand B,Sivaprakasam S

更新日期：2019-09-01 00:00:00

abstract：:D-glucuronic acid (GlcUA) is an important intermediate with numerous applications in the food, cosmetics, and pharmaceutical industries. Its biological production routes which employ myo-inositol oxygenase (MIOX) as the key enzyme are attractive. In this study, five diverse MIOX-encoding genes, from Cryptococcus neofo...

journal_title：Enzyme and microbial technology

authors： Teng F,You R,Hu M,Liu W,Wang L,Tao Y

更新日期：2019-08-01 00:00:00

abstract：:A solvent-mediated method (SMM) was used to prepare supersaturated sugar solutions in hydrophobic and mixture of hydrophilic/hydrophobic ionic liquids (ILs), namely, [Bmim][Tf2N] and [Bmim][TfO]/[Bmim][Tf2N], respectively. In this method, sugars were first solubilized in a mixture of organic solvent and water (i.e. me...

journal_title：Enzyme and microbial technology

authors： Shin DW,Mai NL,Bae SW,Koo YM

更新日期：2019-07-01 00:00:00

abstract：:The cell wall integrity and stress response component (WSC) domain was first described in the Wsc-family protein of the yeast Saccharomyces cerevisiae, and later found in diverse eukaryotic organisms. Due solely to their presence in the Wsc-family proteins working as a plasma membrane sensor for surface stress and in ...

journal_title：Enzyme and microbial technology

authors： Oide S,Tanaka Y,Watanabe A,Inui M

更新日期：2019-06-01 00:00:00

abstract：:The effect of intense pulsed light (IPL) irradiation on Chromobacterium viscosum lipase was investigated with a primary focus on catalytic activity and molecular structure. During IPL irradiation, lipase activity decreased significantly with increasing pulse fluence (Fp) and exposure time (te). IPL-induced deactivatio...

journal_title：Enzyme and microbial technology

authors： Jeon MS,Park KM,Yu H,Park JY,Chang PS

更新日期：2019-05-01 00:00:00

abstract：:Recently enzymatic catalysts have replaced organic and organometallic catalysts in the synthesis of bio-resorbable polymers. Enzymatic polymerization is considered as an alternative to conventional polymerization as they are less toxic, environmental friendly and can operate under mild conditions. In this research, th...

journal_title：Enzyme and microbial technology

authors： Pakalapati H,Tariq MA,Arumugasamy SK

更新日期：2019-03-01 00:00:00

abstract：:Various recombinant proteins can be produced by the yeast Saccharomyces cerevisiae cell factories; therefore, efficient recombinant protein production techniques are desirable. In this study, to establish an efficient recombinant protein production technique in S. cerevisiae, the secretory production of recombinant pr...

journal_title：Enzyme and microbial technology

authors： Sasaki Y,Mitsui R,Yamada R,Ogino H

更新日期：2019-02-01 00:00:00

abstract：:Marine microbes have gained significant attention as potential biofactories for broad spectrum bioactive compounds. In the recent years, bioactive biosurfactants have warranted renewed interest from both environmental and clinical sectors as anti-biofilm agents due to their excellent properties of dispersing microbial...

journal_title：Enzyme and microbial technology

authors： Balan SS,Mani P,Kumar CG,Jayalakshmi S

更新日期：2019-01-01 00:00:00

abstract：:Thiol dioxygenases are non-heme mononuclear-iron proteins and belong to the cupin superfamily. In 2014, mercaptosuccinate dioxygenase (Msdo) of Variovorax paradoxus B4 was identified as another bacterial cysteine dioxygenase (Cdo) homolog catalyzing the conversion of mercaptosuccinate (MS) into succinate and sulfite. ...

journal_title：Enzyme and microbial technology

authors： Brandt U,Galant G,Meinert-Berning C,Steinbüchel A

更新日期：2019-01-01 00:00:00

abstract：:Three threonine aldolases (TAs) were cloned and overexpressed in Escherichia coli (Aeromonas jandaeil-allo-threonine aldolase, E. colil-threonine aldolase and Thermotoga maritimal-allo-threonine aldolase). A Design of Experiments strategy was used to identify optimal reaction conditions for each enzyme. These conditio...

journal_title：Enzyme and microbial technology

authors： Beaudoin SF,Hanna MP,Ghiviriga I,Stewart JD

更新日期：2018-12-01 00:00:00

abstract：:Glutaric acid is one of the promising C5 platform compounds in the biochemical industry. It can be produced chemically, through the ring-opening of butyrolactone followed by hydrolysis. Alternatively, glutaric acid can be produced via lysine degradation pathways by microorganisms. In microorganisms, the overexpression...

journal_title：Enzyme and microbial technology

authors： Hong YG,Moon YM,Hong JW,No SY,Choi TR,Jung HR,Yang SY,Bhatia SK,Ahn JO,Park KM,Yang YH

更新日期：2018-11-01 00:00:00

abstract：:A method to stably immobilize microbes on electrodes was developed. Resting cells of Methylobacterium extorquens AM1(MeAM1) were caged within multiwalled carbon nanotubes (MWNTs)by adding the cells to a water dispersion of MWNTs then allowing the resulting mixture to dry on electrodes. The MeAM1-MWCNTs electrode thus ...

journal_title：Enzyme and microbial technology

authors： Xia HQ,Sakai K,Kitazumi Y,Shirai O,Takagi K,Kano K

更新日期：2018-10-01 00:00:00

abstract：:A novel kinetic method was developed for the quantitation of α-ketoglutaric acid (AKG) in cardioplegic solution and athletic supplements. The assay relied on an enzymatic transamination of AKG and d-4-hydroxyphenylglycine to form 4-hydroxybenzoylformic acid and l-glutamic acid using d-phenylglycine aminotransferase. S...

journal_title：Enzyme and microbial technology

authors： Rojanarata T,Laiwattanapaisal W,Ngawhirunpat T,Opanasopit P,Worrawethanakul K,Chantadee T,Fuangwattana T

更新日期：2018-09-01 00:00:00