UV-Vis detection of hydrogen peroxide using horseradish peroxidase/copper phosphate hybrid nanoflowers.

Abstract:

:A colorimetric platform based on enzyme-inorganic hybrid nanoflowers was used for ultraviolet-visible detection of hydrogen peroxide (H2O2). The hybrid nanoflowers were prepared via incubation at room temperature using horseradish peroxidase (HRP) and copper phosphate (Cu3(PO4)2) as the organic and inorganic components, respectively. HRP/Cu3(PO4)2 hybrid nanoflowers (HRP-HNFs) showed linear H2O2 detection range from 100 nM to 100 μM, moreover, HRP-HNFs exhibited good selectivity for H2O2 detection due to the specificity of HRP. In addition, HRP-HNFs have good reusability, excellent color stability and long-term storage stability, which indicate HRP-HNFs are promising for catalysis and biomedical applications.

journal_name

Enzyme Microb Technol

authors

Yang C,Zhang M,Wang W,Wang Y,Tang J

doi

10.1016/j.enzmictec.2020.109620

subject

Has Abstract

pub_date

2020-10-01 00:00:00

pages

109620

eissn

0141-0229

issn

1879-0909

pii

S0141-0229(20)30113-7

journal_volume

140

pub_type

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