UV-Vis detection of hydrogen peroxide using horseradish peroxidase/copper phosphate hybrid nanoflowers.


:A colorimetric platform based on enzyme-inorganic hybrid nanoflowers was used for ultraviolet-visible detection of hydrogen peroxide (H2O2). The hybrid nanoflowers were prepared via incubation at room temperature using horseradish peroxidase (HRP) and copper phosphate (Cu3(PO4)2) as the organic and inorganic components, respectively. HRP/Cu3(PO4)2 hybrid nanoflowers (HRP-HNFs) showed linear H2O2 detection range from 100 nM to 100 μM, moreover, HRP-HNFs exhibited good selectivity for H2O2 detection due to the specificity of HRP. In addition, HRP-HNFs have good reusability, excellent color stability and long-term storage stability, which indicate HRP-HNFs are promising for catalysis and biomedical applications.


Enzyme Microb Technol


Yang C,Zhang M,Wang W,Wang Y,Tang J




Has Abstract


2020-10-01 00:00:00












  • Effect of threonine, cystathionine, and the branched-chain amino acids on the metabolism of Zygosaccharomyces rouxii*

    abstract::Zygosaccharomyces rouxii is an important yeast in the formation of flavor in soy sauce. In this study, we investigated the separate effects of exogenous threonine, cystathionine, and the branched-chain amino acids on the metabolism of Z. rouxii. The addition of these amino acids had significant effects on both Z. roux...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: van der Sluis C,Wolken WA,Giuseppin ML,Tramper J,Wijffels RH

    更新日期:2000-02-01 00:00:00

  • Structural characterization and biological evaluation of Staphylosan (dimannooleate), a new glycolipid surfactant produced by a marine Staphylococcus saprophyticus SBPS-15.

    abstract::Marine microbes have gained significant attention as potential biofactories for broad spectrum bioactive compounds. In the recent years, bioactive biosurfactants have warranted renewed interest from both environmental and clinical sectors as anti-biofilm agents due to their excellent properties of dispersing microbial...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Balan SS,Mani P,Kumar CG,Jayalakshmi S

    更新日期:2019-01-01 00:00:00

  • Progress in using threonine aldolases for preparative synthesis.

    abstract::Three threonine aldolases (TAs) were cloned and overexpressed in Escherichia coli (Aeromonas jandaeil-allo-threonine aldolase, E. colil-threonine aldolase and Thermotoga maritimal-allo-threonine aldolase). A Design of Experiments strategy was used to identify optimal reaction conditions for each enzyme. These conditio...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Beaudoin SF,Hanna MP,Ghiviriga I,Stewart JD

    更新日期:2018-12-01 00:00:00

  • Functional analysis of active amino acid residues of the mercaptosuccinate dioxygenase of Variovorax paradoxus B4.

    abstract::Thiol dioxygenases are non-heme mononuclear-iron proteins and belong to the cupin superfamily. In 2014, mercaptosuccinate dioxygenase (Msdo) of Variovorax paradoxus B4 was identified as another bacterial cysteine dioxygenase (Cdo) homolog catalyzing the conversion of mercaptosuccinate (MS) into succinate and sulfite. ...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Brandt U,Galant G,Meinert-Berning C,Steinbüchel A

    更新日期:2019-01-01 00:00:00

  • Stabilization of Penicillium occitanis cellulases by spray drying in presence of Maltodextrin.

    abstract::The stabilization of fungal cellulases by spray drying, the thermal stability of Penicillium occitanis cellulases and the effect of some additives were studied. We observed that CMCase activity presents a good stability at 50 degrees C, even after 60 h of incubation. On the other hand, beta-glucosidase activity was mo...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Belghith H,Ellouz Chaabouni S,Gargouri A

    更新日期:2001-02-01 00:00:00

  • Enzymatic production of cephalexin.

    abstract::Enzymatic production of cephalexin using immobilized penicillin G acylase was studied. The factors that affect the conversion of 7-amino-3-deacetoxycephalosporanic acid (7-ADCA) to cephalexin, such as pH; temperature; concentrations of 7-ADCA, D(-)-phenylglycine methyl ester (PGME), and immobilized penicillin G acylas...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Maladkar NK

    更新日期:1994-08-01 00:00:00

  • Course of pH during the formation of amoxicillin by a suspension-to-suspension reaction.

    abstract::Amoxicillin can be produced in an enzymatic suspension-to-suspension reaction in which the substrate(s) and product(s) are mainly present as solid particles, while the reaction takes place in the liquid phase. During these suspension-to-suspension reactions different subprocesses take place, such as dissolution/crysta...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Diender MB,Straathof AJ,van der Does T,Zomerdijk M,Heijnen JJ

    更新日期:2000-11-01 00:00:00

  • CAH1 and CAH2 as key enzymes required for high bicarbonate tolerance of a novel microalga Dunaliella salina HTBS.

    abstract::Outdoor microalgal cultivation with high concentration bicarbonate has been considered as a strategy for reducing contamination and improving carbon supply efficiency. The mechanism responsible for algae's strong tolerance to high bicarbonate however, remains not clear. In this study, we isolated and characterized a s...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Hou Y,Liu Z,Zhao Y,Chen S,Zheng Y,Chen F

    更新日期:2016-06-01 00:00:00

  • Production of glutaric acid from 5-aminovaleric acid using Escherichia coli whole cell bio-catalyst overexpressing GabTD from Bacillus subtilis.

    abstract::Glutaric acid is one of the promising C5 platform compounds in the biochemical industry. It can be produced chemically, through the ring-opening of butyrolactone followed by hydrolysis. Alternatively, glutaric acid can be produced via lysine degradation pathways by microorganisms. In microorganisms, the overexpression...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Hong YG,Moon YM,Hong JW,No SY,Choi TR,Jung HR,Yang SY,Bhatia SK,Ahn JO,Park KM,Yang YH

    更新日期:2018-11-01 00:00:00

  • A sialic acid aldolase from Peptoclostridium difficile NAP08 with 4-hydroxy-2-oxo-pentanoate aldolase activity.

    abstract::Sialic acid aldolases (E.C. catalyze the reversible aldol cleavage of N-acetyl-d-neuraminic acid (Neu5Ac) to from N-acetyl-d-mannosamine (ManNAc) and pyruvate. In this study, a sialic acid aldolase (PdNAL) from Peptoclostridium difficile NAP08 was expressed in Escherichia coli BL21 (DE3). This homotetrameric e...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Chen Q,Han L,Chen X,Cui Y,Feng J,Wu Q,Zhu D

    更新日期:2016-10-01 00:00:00

  • Carbon-nanotube-caged microbial electrodes for bioelectrocatalysis.

    abstract::A method to stably immobilize microbes on electrodes was developed. Resting cells of Methylobacterium extorquens AM1(MeAM1) were caged within multiwalled carbon nanotubes (MWNTs)by adding the cells to a water dispersion of MWNTs then allowing the resulting mixture to dry on electrodes. The MeAM1-MWCNTs electrode thus ...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Xia HQ,Sakai K,Kitazumi Y,Shirai O,Takagi K,Kano K

    更新日期:2018-10-01 00:00:00

  • Transient behavior of T-cell hybridomas in response to changes in metabolite concentrations in continuous culture.

    abstract::Effects of pulse and step changes in major metabolite concentrations such as glucose, glutamine, lactic acid, and ammonium on behavior of T-cell hybridomas in continuous culture were investigated in order to develop a strategy to maximize specific rate of lymphokine formation. Step increases in glucose, lactic acid, a...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Shaughnessy TS,Kargi F

    更新日期:1990-09-01 00:00:00

  • Enzymatic synthesis of phenethyl ester from phenethyl alcohol with acyl donors.

    abstract::Phenethyl ester is an important contributors to food, drinks, cosmetics and perfumery. Phenethyl acetate was synthesized enzymatically from phenethyl alcohol with acyl donors catalyzed by Novozym 435 from Candida antarctica lipase B. Through acyl donor screening, acetic anhydride and vinyl acetate were found to be the...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Kim H,Park C

    更新日期:2017-05-01 00:00:00

  • Effects of non-ionic surfactants on the interactions between cellulases and tannic acid: a model system for cellulase-poly-phenol interactions.

    abstract::Addition of non-ionic surfactants (NIS) is known to accelerate enzymatic lignocellulose hydrolysis. The mechanism behind this accelerating effect is still not elucidated but has been hypothesized to originate from favorable NIS-lignin interactions which alleviate non-productive adsorption of cellulases to lignin. In t...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Olsen SN,Bohlin C,Murphy L,Borch K,McFarland KC,Sweeny MD,Westh P

    更新日期:2011-09-10 00:00:00

  • Increasing the refolding efficiency in vitro by site-directed mutagenesis of Cys383 in rat procarboxypeptidase B.

    abstract::This study examines a novel method to reduce the probability of disulfide mismatches during the refolding process by the replacement of cysteines within a protein. Specifically, Cys383 of recombinant rat procarboxypeptidase B was replaced by other amino acids to increase the refolding efficiency in vitro. Mutants C383...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Li S,Zhang L,Wu Q,Xin A,Zhao J,Fan L

    更新日期:2011-07-10 00:00:00

  • Secretory overexpression of the endoglucanase by Saccharomyces cerevisiae via CRISPR-δ-integration and multiple promoter shuffling.

    abstract::Various recombinant proteins can be produced by the yeast Saccharomyces cerevisiae cell factories; therefore, efficient recombinant protein production techniques are desirable. In this study, to establish an efficient recombinant protein production technique in S. cerevisiae, the secretory production of recombinant pr...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Sasaki Y,Mitsui R,Yamada R,Ogino H

    更新日期:2019-02-01 00:00:00

  • Engineering the central pathways in Lactococcus lactis: functional expression of the phosphofructokinase (pfk) and alternative oxidase (aox1) genes from Aspergillus niger in Lactococcus lactis facilitates improved carbon conversion rates under oxidizing c

    abstract::The present work describes a novel central pathway engineering method that has been designed with the aim to increase the carbon conversion rates under oxidizing conditions in L. lactis fermentations. The nisin producer L. lactis ATCC11454 strain has been genetically engineered by cloning a truncated version of the ph...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Papagianni M,Avramidis N

    更新日期:2012-08-10 00:00:00

  • Structural and functional consequences of replacement of His403 with Arg near the catalytic site of Anoxybacillus flavithermus cyclomaltodextrinase.

    abstract::The hydrolytic activity of a thermophilic cyclomaltodextrinase (CMD) from Anoxybacillus flavithermus ZNU-NGA and a representative single mutant were investigated against soluble substrates including α-, β- and γ-cyclomaltodestrines (CDs). Based on the occurrence of arginine (Arg) at position 403 in some homologue prot...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Mirzaee Z,Jafarian V,Shirdel SA,Khalifeh K

    更新日期:2019-12-01 00:00:00

  • Dynamics of proteolysis and its influence on the accumulation of intracellular recombinant proteins.

    abstract::A method to quantify the impact of proteolysis on accumulation of recombinant proteins in E. coli is described. A much smaller intracellular concentration of staphylococcal protein A (SpA) (14.7 mg. g(-1)) compared to the fusion protein SpA-betagalactosidase (138 mg. g(-1)) is explained by a very high proteolysis rate...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Rozkov A,Schweder T,Veide A,Enfors S

    更新日期:2000-12-01 00:00:00

  • Enhanced lipase-catalyzed synthesis of sugar fatty acid esters using supersaturated sugar solution in ionic liquids.

    abstract::A solvent-mediated method (SMM) was used to prepare supersaturated sugar solutions in hydrophobic and mixture of hydrophilic/hydrophobic ionic liquids (ILs), namely, [Bmim][Tf2N] and [Bmim][TfO]/[Bmim][Tf2N], respectively. In this method, sugars were first solubilized in a mixture of organic solvent and water (i.e. me...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Shin DW,Mai NL,Bae SW,Koo YM

    更新日期:2019-07-01 00:00:00

  • Combinational expression of D-sorbitol dehydrogenase and pyrroloquinoline quinone increases 6-(N-hydroxyethyl)-amino-6-deoxy-α-L-sorbofuranose production by Gluconobacter oxydans through cofactor manipulation.

    abstract::6-(N-hydroxyethyl)-amino-6-deoxy-l-sorbofuranose (6NSL), a key precursor in the synthesis of miglitol, is produced from N-2-hydroxyethyl-glucamine (NHEG) by the regioselective oxidation of Gluconobacter oxydans. The limitation of PQQ biosynthesis became a bottleneck for improvement of PQQ-dependent D-sorbitol dehydrog...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Liu D,Ke X,Hu ZC,Zheng YG

    更新日期:2020-11-01 00:00:00

  • Improved thermostability and enzyme activity of a recombinant phyA mutant phytase from Aspergillus niger N25 by directed evolution and site-directed mutagenesis.

    abstract::We previously constructed three recombinant phyA mutant strains (PP-NPm-8, PP-NPep-6A and I44E/T252R-PhyA), showing improved catalytic efficiency or thermostability of Aspergillus niger N25 phytase, by error-prone PCR or site-directed mutagenesis. In this study, directed evolution and site-directed mutagenesis were fu...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Tang Z,Jin W,Sun R,Liao Y,Zhen T,Chen H,Wu Q,Gou L,Li C

    更新日期:2018-01-01 00:00:00

  • The kinetics of affinity-mediated cell-surface attachment.

    abstract::Data and a semi-empirical model are presented that describe the affinity interaction of yeast cells with a Concanavalin A derivatised surface. The model uses 3 parameters to describe the time course of cell attachment from a flowing suspension of yeast cells, over a range of flow rates, and gives an effective global f...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Ming F,Eisenthal R,Whish WJ,Hubble J

    更新日期:2000-02-01 00:00:00

  • The influence of hexoses addition on the fermentation of d-xylose in Debaryomyces hansenii under continuous cultivation.

    abstract::The effect of hexoses (glucose and galactose) addition to the feed xylose mineral medium of Debaryomyces hansenii chemostat cultures grown at a constant dilution rate of 0.055 h(-1) was studied. Xylitol was the major product detected amongst all tested conditions. The maximal values for xylitol yield and volumetric pr...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Tavares JM,Duarte LC,Amaral-Collaço MT,Gírio FM

    更新日期:2000-06-01 00:00:00

  • Protein nano-crystallogenesis.

    abstract::We demonstrate the feasibility of growing crystals of protein in volumes as small as 1 nanoliter. Advances in the handling of very small volumes (i.e. through inkjet and other technologies) open the way towards fully automated systems. The rationale for these experiments is the desire to develop a system that speeds u...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Kuil ME,Bodenstaff ER,Hoedemaeker FJ,Abrahams JP

    更新日期:2002-03-13 00:00:00

  • Screening and identification of five peptides from pinto bean with inhibitory activities against α-amylase using phage display technique.

    abstract::The objective of this study was to screen and identify α-amylase inhibitor peptides from Pinto bean. Five Pinto bean bioactive peptides were successfully identified: PPHMLP (P1), PLPWGAGF (P3), PPHMGGP (P6), PLPLHMLP (P7) and LSSLEMGSLGALFVCM (P9). Based on ELISA results, their promising optical density values were 1....

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Ngoh YY,Lim TS,Gan CY

    更新日期:2016-07-01 00:00:00

  • Aspergillus niger I-1472 and Pycnoporus cinnabarinus MUCL39533, selected for the biotransformation of ferulic acid to vanillin, are also able to produce cell wall polysaccharide-degrading enzymes and feruloyl esterases.

    abstract::The filamentous fungal strains Aspergillus niger I-1472 and Pycnoporus cinnabarinus MUCL39533, previously selected for the bioconversion of ferulic acid to vanillic acid and vanillin respectively, were grown on sugar beet pulp. A large spectrum of polysaccharide-degrading enzymes was produced by A. niger and very few ...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Bonnina E,Brunel M,Gouy Y,Lesage-Meessen L,Asther M,Thibault J

    更新日期:2001-01-02 00:00:00

  • Effect of intense pulsed light on the deactivation of lipase: Enzyme-deactivation kinetics and tertiary structural changes by fragmentation.

    abstract::The effect of intense pulsed light (IPL) irradiation on Chromobacterium viscosum lipase was investigated with a primary focus on catalytic activity and molecular structure. During IPL irradiation, lipase activity decreased significantly with increasing pulse fluence (Fp) and exposure time (te). IPL-induced deactivatio...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Jeon MS,Park KM,Yu H,Park JY,Chang PS

    更新日期:2019-05-01 00:00:00

  • Genetic engineering of Trichoderma to produce strains with novel cellulase profiles.

    abstract::Genetic engineering has been used to modify the proportion of different cellulases produced by a hypercellulolytic Trichoderma reesei mutant strain. A general expression vector, pAMH110, containing the promoter and terminator sequences of the strongly expressed main cellobiohydrolase 1 (cbh1) gene was used to overexpr...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Harkki A,Mäntylä A,Penttilä M,Muttilainen S,Bühler R,Suominen P,Knowles J,Nevalainen H

    更新日期:1991-03-01 00:00:00

  • Enzymatic cascade biosynthesis reaction of musky macrolactones from fatty acids.

    abstract::Musky macrolactones are an important group of compounds used in high-valued perfumery. An enzymatic cascade reaction including cytochrome P450 hydroxylase and lipase was explored to biosynthesize musky macrolactones. Firstly, fatty acids were hydroxylated by P450 hydroxylase to produce the corresponding ω-hydroxy fatt...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Meng S,Guo J,Li Z,Nie K,Xu H,Tan T,Liu L

    更新日期:2019-12-01 00:00:00