A single and two step isomerization process for d-tagatose and l-ribose bioproduction using l-arabinose isomerase and d-lyxose isomerase.

abstract：:Kinetics of nitrification and denitrification of synthetic wastewater was investigated by using two reactors in series. An activated sludge unit was used for nitrification followed by a downflow biofilter for denitrification. Glucose solution was fed to the denitrification column to supply carbon source. Reactors were...

journal_title：Enzyme and microbial technology

authors： Dinçer AR,Kargi F

更新日期：2000-07-01 00:00:00

abstract：:Escherichia coli B/pTG201 recombinant cells were immobilized by entrapment in a carrageenan gel and cultivated in nonselective media to investigate the effect of agitation rate on plasmid stability, biomass concentration, and enzyme productivity. These parameters were studied in continuous cultures for free and immobi...

journal_title：Enzyme and microbial technology

authors： Huang J,Dhulster P,Thomas D,Barbotin JN

更新日期：1990-12-01 00:00:00

abstract：:beta-Xylosidase (1,4-beta-D-xylan xylohydrolase EC 3.2.1.37) and xylose isomerase (D-xylose ketol-isomerase EC 5.3.1.5) produced by Streptomyces sp. strain EC 10, were cell-bound enzymes induced by xylan, straw, and xylose. Enzyme production was subjected to a form of carbon catabolite repression by glycerol. beta-Xyl...

journal_title：Enzyme and microbial technology

authors： Belfaquih N,Penninckx MJ

更新日期：2000-07-01 00:00:00

abstract：:The methyl ester of 2-benzoxazolon-3-yl-acetic acid was used as an acyl donor in the penicillin amidase-catalysed transfer reaction to 7-aminodesacetoxycephalosporanic acid. The synthesis of 7-(2-benzoxazolon-3-yl-acetamido)-desacetoxycephalosporanic acid was carried out as a kinetically controlled reaction. A charact...

journal_title：Enzyme and microbial technology

authors： Stambolieva N,Mincheva Z,Galunsky B,Kalcheva V

更新日期：1992-06-01 00:00:00

abstract：:The cell wall integrity and stress response component (WSC) domain was first described in the Wsc-family protein of the yeast Saccharomyces cerevisiae, and later found in diverse eukaryotic organisms. Due solely to their presence in the Wsc-family proteins working as a plasma membrane sensor for surface stress and in ...

journal_title：Enzyme and microbial technology

authors： Oide S,Tanaka Y,Watanabe A,Inui M

更新日期：2019-06-01 00:00:00

abstract：:Amoxicillin can be produced in an enzymatic suspension-to-suspension reaction in which the substrate(s) and product(s) are mainly present as solid particles, while the reaction takes place in the liquid phase. During these suspension-to-suspension reactions different subprocesses take place, such as dissolution/crysta...

journal_title：Enzyme and microbial technology

authors： Diender MB,Straathof AJ,van der Does T,Zomerdijk M,Heijnen JJ

更新日期：2000-11-01 00:00:00

abstract：:Different carbon sources affecting growth and lipase production in Candida rugosa were studied by using batch cultures on defined medium. Carbohydrates and acids non-related to fats did not induce lipase production. The highest yields of enzyme were obtained with lipids or fatty acids as carbon sources. Tween 80 stimu...

journal_title：Enzyme and microbial technology

authors： Dalmau E,Montesinos JL,Lotti M,Casas C

更新日期：2000-06-01 00:00:00

abstract：:This study reports a general strategy for the encapsulation of various enzymes in amphiphilic hollow carbonaceous microspheres (CMs). We found that enzymes could be spontaneously encapsulated in the interior cavity of the CMs via hydrophobic interactions. Due to strong hydrophobic interactions and robust confinement, ...

journal_title：Enzyme and microbial technology

authors： Liu L,Sun Y,He L,Jiang L,Yang S

更新日期：2015-11-01 00:00:00

abstract：:When dissolved oxygen (DO) was maintained at saturation level during batch fermentations of Streptomyces clavuligerus (NRRL 3585), the accumulation of the intermediate penicillin N was lowered while formation of the end product cephamycin C was increased relative to fermentations without DO control. The specific activ...

journal_title：Enzyme and microbial technology

authors： Rollins MJ,Jensen SE,Wolfe S,Westlake DW

更新日期：1990-01-01 00:00:00

abstract：:Advantages in maltose fermentation at extremely low temperatures (5-10°C) using an alcohol resistant and cryotolerant yeast strain (Saccharomyces cerevisiae AXAZ-1) immobilized on porous cellulosic material (or tubular cellulose, TC), produced by delignification of wood sawdust, are reported. Pure maltose and glucose ...

journal_title：Enzyme and microbial technology

authors： Ganatsios V,Koutinas AA,Bekatorou A,Kanellaki M,Nigam P

更新日期：2014-11-01 00:00:00

abstract：:Filamentous fungal secretomes comprise highly dynamic sets of proteins, including multiple carbohydrate active enzymes (CAZymes) which are able to hydrolyze plant biomass polysaccharides into products of biotechnological interest such as fermentable sugars. In recent years, proteomics has been used to identify and qua...

journal_title：Enzyme and microbial technology

authors： Cologna NMD,Gómez-Mendoza DP,Zanoelo FF,Giannesi GC,Guimarães NCA,Moreira LRS,Filho EXF,Ricart CAO

更新日期：2018-02-01 00:00:00

abstract：:Before applying nanotechnologies in biomedical and environmental areas it is advised to study interactions of nanoparticles and other nanomaterials with biomacromolecule present in living system. Moreover there is scarcity of reports on interactions between nanoparticles and biomaterials. In present report a rapid, ec...

journal_title：Enzyme and microbial technology

authors： Patil CD,Borase HP,Suryawanshi RK,Patil SV

更新日期：2016-10-01 00:00:00

abstract：:In this work, porous biochar derived from sugarcane bagasse was prepared and then coated with different amounts of chitosan (C@CS) for cellulase immobilization. Cellulase was covalently immobilized on the support by using glutaraldehyde as a linker. The chemical characteristics and morphology of the samples were deter...

journal_title：Enzyme and microbial technology

authors： Mo H,Qiu J,Yang C,Zang L,Sakai E,Chen J

更新日期：2020-08-01 00:00:00

abstract：:The D-amino acid oxidase cDNA gene (daao) of Trigonopsis variabilis was prepared by reverse transcriptase-polymerase chain reaction (PCR) and cloned into Escherichia coli expression vector, pTrc99A, under the control of tac promoter. Expression of daao gene significantly affected the growth and morphology of E. coli. ...

journal_title：Enzyme and microbial technology

authors： Lin L,Chien HR,Wang W,Hwang T,Fu H,Hsu W

更新日期：2000-10-01 00:00:00

abstract：:Magnetosomes are intracellular structures produced by magnetotactic bacteria and are magnetic nanoparticles surrounded by a lipid bilayer membrane. Magnetosomes reportedly possess intrinsic enzyme mimetic activity similar to that found in horseradish peroxidase (HRP) and can scavenge reactive oxygen species depending ...

journal_title：Enzyme and microbial technology

authors： Li K,Chen C,Chen C,Wang Y,Wei Z,Pan W,Song T

更新日期：2015-05-01 00:00:00

abstract：:This study examines a novel method to reduce the probability of disulfide mismatches during the refolding process by the replacement of cysteines within a protein. Specifically, Cys383 of recombinant rat procarboxypeptidase B was replaced by other amino acids to increase the refolding efficiency in vitro. Mutants C383...

journal_title：Enzyme and microbial technology

authors： Li S,Zhang L,Wu Q,Xin A,Zhao J,Fan L

更新日期：2011-07-10 00:00:00

abstract：:6-(N-hydroxyethyl)-amino-6-deoxy-l-sorbofuranose (6NSL), a key precursor in the synthesis of miglitol, is produced from N-2-hydroxyethyl-glucamine (NHEG) by the regioselective oxidation of Gluconobacter oxydans. The limitation of PQQ biosynthesis became a bottleneck for improvement of PQQ-dependent D-sorbitol dehydrog...

journal_title：Enzyme and microbial technology

authors： Liu D,Ke X,Hu ZC,Zheng YG

更新日期：2020-11-01 00:00:00

abstract：:We demonstrate the feasibility of growing crystals of protein in volumes as small as 1 nanoliter. Advances in the handling of very small volumes (i.e. through inkjet and other technologies) open the way towards fully automated systems. The rationale for these experiments is the desire to develop a system that speeds u...

journal_title：Enzyme and microbial technology

authors： Kuil ME,Bodenstaff ER,Hoedemaeker FJ,Abrahams JP

更新日期：2002-03-13 00:00:00

abstract：:A novel endoglucanase active on 1,3-1,4-beta-D-glucans was purified to apparent homogeneity from submerged cultures of the moderately thermophilic aerobic fungus Talaromyces emersonii CBS 814.70. The enzyme is a single subunit glycoprotein with M(r) and pI values of 40.7 +/- 0.3 kDa and 4.4, respectively, and an estim...

journal_title：Enzyme and microbial technology

authors： Murray PG,Grassick A,Laffey CD,Cuffe MM,Higgins T,Savage AV,Planas A,Tuohy MG

更新日期：2001-07-05 00:00:00

abstract：:Continuous culture and fed-batch fermentations were used to test the behavior of the system Bacillus subtilis DN1885(pCH7) that synthesizes a recombinant beta-1,4-endoglucanase. Continuous culture experiments were focused on the study of the instability aspects of the system as well as determination of the biomass gro...

journal_title：Enzyme and microbial technology

authors： Shene C,Mir N,Andrews BA,Asenjo JA

更新日期：2000-08-01 00:00:00

abstract：:The hydrolytic activity of a thermophilic cyclomaltodextrinase (CMD) from Anoxybacillus flavithermus ZNU-NGA and a representative single mutant were investigated against soluble substrates including α-, β- and γ-cyclomaltodestrines (CDs). Based on the occurrence of arginine (Arg) at position 403 in some homologue prot...

journal_title：Enzyme and microbial technology

authors： Mirzaee Z,Jafarian V,Shirdel SA,Khalifeh K

更新日期：2019-12-01 00:00:00

abstract：:Laccases (benzenediol oxygen oxidoreductases, EC 1.10.3.2) are important multicopper enzymes that are used in many biotechnological processes. A recombinant form of laccase from Bacillus sp. HR03 was overexpressed in Escherichia coli BL-21(DE3). Inclusion body (IB) formation happens quite often during recombinant prot...

journal_title：Enzyme and microbial technology

authors： Mollania N,Khajeh K,Ranjbar B,Rashno F,Akbari N,Fathi-Roudsari M

更新日期：2013-05-10 00:00:00

abstract：:An extracellular esterase from the actinomycetes Amycolatopsis orientalis was found by screening. It is capable of splitting the isomeric mixture (K/J) of (I, Scheme 1) into 7-amino-3-methoxymethyl-3-cephem-4-carboxylic acid, pivalic acid, and acetaldehyde with a high yield. The purified enzyme of 55.4 Kd by SDS-PAGE ...

journal_title：Enzyme and microbial technology

authors： Sauber K,Aretz W,Meiwes J,Wollmann T

更新日期：1996-07-01 00:00:00

abstract：:Anthocyanin production by strawberry cells depends not only on light intensity but also on the light/dark cycle operation with hour- or second-scale periods. These findings are useful for designing and operating photobioreactors for enhanced anthocyanin production. Intermittent illumination with a second-scale period ...

journal_title：Enzyme and microbial technology

authors： Kurata H,Mochizuki A,Okuda N,Seki M,Furusaki S

更新日期：2000-05-01 00:00:00

abstract：:The mechanical properties of TB/C3 hybridoma cells taken from a continuous culture were measured by micromanipulation. The culture conditions were constant except for the presence or absence of Pluronic F-68 in the medium. It was found that the mean bursting membrane tension and the mean elastic area compressibility m...

journal_title：Enzyme and microbial technology

authors： Zhang Z,al-Rubeai M,Thomas CR

更新日期：1992-12-01 00:00:00

abstract：:The effect of treatment with various intercalating dyes on the ability to produce antibiotics in Micromonospora rosaria and Micromonospora purpurea was studied. Treatment with acriflavine resulted in a high frequency loss of antibiotic productivity in both species. In M. rosaria, the loss of antibiotic-producing abili...

journal_title：Enzyme and microbial technology

authors： Kim KS,Pai HS,Lee SY,Ryu DD

更新日期：1990-08-01 00:00:00

abstract：:The possibility of using thermostable inulinases from Aspergillus ficuum in place of invertase for sucrose hydrolysis was explored. The commercial inulinases preparation was immobilized onto porous glass beads by covalent coupling using activation by a silane reagent and glutaraldehyde before adding the enzyme. The im...

journal_title：Enzyme and microbial technology

authors： Ettalibi M,Baratti JC

更新日期：2001-05-07 00:00:00

abstract：:Sialic acid aldolases (E.C.4.1.3.3) catalyze the reversible aldol cleavage of N-acetyl-d-neuraminic acid (Neu5Ac) to from N-acetyl-d-mannosamine (ManNAc) and pyruvate. In this study, a sialic acid aldolase (PdNAL) from Peptoclostridium difficile NAP08 was expressed in Escherichia coli BL21 (DE3). This homotetrameric e...

journal_title：Enzyme and microbial technology

authors： Chen Q,Han L,Chen X,Cui Y,Feng J,Wu Q,Zhu D

更新日期：2016-10-01 00:00:00

abstract：:Two GH43 β-xylosidases, RS223-BX from a rice straw metagenomic library, and BoXA from Bacteroides ovatus, that share similar amino acid sequences (81% identical) and 19 of 20 active-site residues, were compared by using site-directed mutagenesis of Asp and His residues implicated in metal binding. Thus, RS223-BX is st...

journal_title：Enzyme and microbial technology

authors： Jordan DB,Stoller JR,Kibblewhite RE,Chan VJ,Lee CC,Wagschal K

更新日期：2018-07-01 00:00:00

abstract：:An improved amperometric l-lactate biosensor was constructed based on covalent immobilization of lactate oxidase (LOx) from Pediococcus species onto carboxylated multiwalled carbon nanotubes (cMWCNT)/copper nanoparticles (CuNPs)/polyaniline (PANI) hybrid film electrodeposited on the surface of a pencil graphite electr...

journal_title：Enzyme and microbial technology

authors： Dagar K,Pundir CS

更新日期：2017-01-01 00:00:00