Abstract:
:An extracellular esterase from the actinomycetes Amycolatopsis orientalis was found by screening. It is capable of splitting the isomeric mixture (K/J) of (I, Scheme 1) into 7-amino-3-methoxymethyl-3-cephem-4-carboxylic acid, pivalic acid, and acetaldehyde with a high yield. The purified enzyme of 55.4 Kd by SDS-PAGE shows an N-terminal sequence of VRTCADLVRTYDLPGAVTH. The isoelectric point is 8.9 +/- 0.1. It can be immobilized with good yield to VA-Epoxy Biosynth. Besides the above-mentioned reaction, the esterase cleaves many other esters such as methyl-2-chloropropionic acid.
journal_name
Enzyme Microb Technoljournal_title
Enzyme and microbial technologyauthors
Sauber K,Aretz W,Meiwes J,Wollmann Tdoi
10.1016/0141-0229(95)00171-9subject
Has Abstractpub_date
1996-07-01 00:00:00pages
15-9issue
1eissn
0141-0229issn
1879-0909pii
0141022995001719journal_volume
19pub_type
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journal_title:Enzyme and microbial technology
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journal_title:Enzyme and microbial technology
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journal_title:Enzyme and microbial technology
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journal_title:Enzyme and microbial technology
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journal_title:Enzyme and microbial technology
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