Abstract:
:Although the chaperon solvent plug was reported as a strategy to reduce aggregation before the column inlet in SEC (size-exclusion chromatography) protein refolding process, the appropriate position at which sample injected and the volume of the chaperon solvent plug have not been elucidated. Therefore, the detail of chaperon solvent plug design was investigated in this work. Our results indicated that, to ensure good performances in the SEC refolding process, the appropriate front and tail volumes of chaperon solvent plug should be slightly larger than the optimal values, which depend on the flow dispersion from the injector to the column inlet. However, with the front volume more than the optimum, it could have an adverse effect on activity recovery but not the mass recovery, while no effect at all if the tail volume exceeded the optimum. Furthermore, it might be economical to replace the eluent (refolding buffer) after the tail of chaperon solvent plug with a cheaper one.
journal_name
Enzyme Microb Technoljournal_title
Enzyme and microbial technologyauthors
Chen YC,Liu HSdoi
10.1016/j.enzmictec.2011.05.003subject
Has Abstractpub_date
2011-07-10 00:00:00pages
203-8issue
2eissn
0141-0229issn
1879-0909pii
S0141-0229(11)00094-9journal_volume
49pub_type
杂志文章abstract::In this study, we report the enzymatic production of glycerol acetate from glycerol and methyl acetate. Lipases are essential for the catalysis of this reaction. To find the optimum conditions for glycerol acetate production, sequential experiments were designed. Type of lipase, lipase concentration, molar ratio of re...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2014.11.004
更新日期:2015-02-01 00:00:00
abstract::In the paper, a green and efficient biosynthetical technique was reported for preparing cadmium sulfide (CdS) quantum dots, in which Escherichia coli (E. coli) was chosen as a biomatrix. Fluorescence emission spectra and fluorescent microscopic photographs revealed that as-produced CdS quantum dots had an optimum fluo...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2016.09.017
更新日期:2017-01-01 00:00:00
abstract::The hydrolytic activity of a thermophilic cyclomaltodextrinase (CMD) from Anoxybacillus flavithermus ZNU-NGA and a representative single mutant were investigated against soluble substrates including α-, β- and γ-cyclomaltodestrines (CDs). Based on the occurrence of arginine (Arg) at position 403 in some homologue prot...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2019.109421
更新日期:2019-12-01 00:00:00
abstract::D-glucuronic acid (GlcUA) is an important intermediate with numerous applications in the food, cosmetics, and pharmaceutical industries. Its biological production routes which employ myo-inositol oxygenase (MIOX) as the key enzyme are attractive. In this study, five diverse MIOX-encoding genes, from Cryptococcus neofo...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2019.04.013
更新日期:2019-08-01 00:00:00
abstract::Effects of pulse and step changes in major metabolite concentrations such as glucose, glutamine, lactic acid, and ammonium on behavior of T-cell hybridomas in continuous culture were investigated in order to develop a strategy to maximize specific rate of lymphokine formation. Step increases in glucose, lactic acid, a...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/0141-0229(90)90007-d
更新日期:1990-09-01 00:00:00
abstract::Acetobacter pasteurianus oxidizes glycidol with high activity, comparable to the oxidation of ethanol. The organism has a preference for the S-enantiomer, and the kinetic resolution process obeys a simple relationship, indicating an enantiomeric ratio (E) of 19. The compound is converted into glycidic acid, although a...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/0141-0229(94)90143-0
更新日期:1994-12-01 00:00:00
abstract::Monod kinetic parameters provide information required for kinetic analysis of anaerobic oxidation of methane coupled to denitrification (AOM-D). This information is critical for engineering AOM-D processes in wastewater treatment facilities. We first experimentally determined Monod kinetic parameters for an AOM-D enri...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2017.05.005
更新日期:2017-09-01 00:00:00
abstract::Musky macrolactones are an important group of compounds used in high-valued perfumery. An enzymatic cascade reaction including cytochrome P450 hydroxylase and lipase was explored to biosynthesize musky macrolactones. Firstly, fatty acids were hydroxylated by P450 hydroxylase to produce the corresponding ω-hydroxy fatt...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2019.109417
更新日期:2019-12-01 00:00:00
abstract::Two novel glycosyl hydrolase family 5 (GH5) β-mannanases (AoMan5A and AoMan5B) were identified from Aspergillus oryzae RIB40 by genome mining. The AoMan5A contains a predicted family 1 carbohydrate binding module (CBM-1), located at its N-terminal. The AoMan5A, AoMan5B and truncated mutant AoMan5AΔCL (truncating the N...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2016.08.003
更新日期:2016-11-01 00:00:00
abstract::Direct esterifications using a nylon-immobilized lipase from Candida cylindracea were carried out in batch and continuous-flow reactors. The immobilized enzyme was effective in catalyzing the synthesis of ethylpropionate, isoamylpropionate, and isoamylbutyrate. With ethanol dissolved in hexane as a substrate, the maxi...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/0141-0229(92)90054-r
更新日期:1992-11-01 00:00:00
abstract::Operational stability under high temperature is required for enzyme application in industrial processes. Error-prone PCR and B-factor analysis were employed to enhance the thermostability of a xylanase from GH family 11 in this study. Based on the top 10 mutants screened from the random mutation libraries, mutant Xyn3...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2020.109720
更新日期:2021-02-01 00:00:00
abstract::Even though many enzyme processes occur at the interface of an insoluble substrate, these reactions are generally much less studied than homogenous enzyme reactions in the aqueous bulk. Interfacial (or heterogeneous) enzyme reactions involve several reaction steps, and the established experimental approach to elucidat...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2017.06.009
更新日期:2017-10-01 00:00:00
abstract::Chemical modification of lysine residues in two bacterial alpha-amylases, a mesophilic enzyme from Bacillus amyloliquefaciens (BAA) and a thermophilic enzyme from Bacillus licheniformis (BLA) was carried out using citraconic anhydride. 13 +/- 1 residues in BAA and 10 +/- 1 residues in BLA were found modified under def...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/s0141-0229(01)00296-4
更新日期:2001-04-05 00:00:00
abstract::Detection of mRNA is a valuable method for monitoring the specific gene expression. In this study, we devised a novel cDNA synthesis method using three enzymes, the genetically engineered thermostable variant of reverse transcriptase (RT), MM4 (E286R/E302K/L435R/D524A) from Moloney murine leukemia virus (MMLV), the ge...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2016.10.003
更新日期:2017-01-01 00:00:00
abstract::Trehalose diesters are Gemini-type surfactants that might have better surface activity than conventional surfactants. A one-step method for the preparation of trehalose unsaturated fatty acid diesters has been successfully developed. The yield of trehalose diester of different unsaturated fatty acids was between 78 % ...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2020.109516
更新日期:2020-05-01 00:00:00
abstract::beta-amylases are used in production of maltose syrup. It is shown that sweet potato beta-amylase can be purified by affinity precipitation with alginate with 80% activity yield and 44 fold purification. SDS-PAGE of the purified protein showed a single band and a subunit weight of 50 kDa. Preliminary data with soybean...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/s0141-0229(01)00338-6
更新日期:2001-06-07 00:00:00
abstract::Two GH43 β-xylosidases, RS223-BX from a rice straw metagenomic library, and BoXA from Bacteroides ovatus, that share similar amino acid sequences (81% identical) and 19 of 20 active-site residues, were compared by using site-directed mutagenesis of Asp and His residues implicated in metal binding. Thus, RS223-BX is st...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2018.03.007
更新日期:2018-07-01 00:00:00
abstract::Outdoor microalgal cultivation with high concentration bicarbonate has been considered as a strategy for reducing contamination and improving carbon supply efficiency. The mechanism responsible for algae's strong tolerance to high bicarbonate however, remains not clear. In this study, we isolated and characterized a s...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2016.02.010
更新日期:2016-06-01 00:00:00
abstract::We have designed an electrochemical DNA biosensor based on stem-loop structured probes for enzymatic detection of Pseudomonas aeruginosa 16S ribosomal RNA (rRNA) in composting degradation. The probe modified with a thiol at its 5' end and a biotin at its 3' end was immobilized on a gold electrode through self-assembly...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2011.06.011
更新日期:2011-08-10 00:00:00
abstract::The present study investigated the influence of low-shear modeled microgravity (LSMMG) conditions on Listeria monocytogenes stress response (heat, cold, and acid), membrane fatty acid composition, and virulence potential as well as stress-/virulence-associated gene expression. The results showed that LSMMG-cultivated ...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2019.109440
更新日期:2020-02-01 00:00:00
abstract::A novel magnetic Fe3O4 nanoparticles (MNPs) coupled with agarose (AMNPs) was synthesized using co-precipitation via alkaline condition and span-80 surfactants in organic solvent. Iminodiacetate was first attached to the MNPs through epichlorohydrin agent and then chelated with metal ions. The morphology and chemical p...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2014.05.008
更新日期:2014-09-01 00:00:00
abstract::This study aimed to develop viable enzymes for bioconversion of resveratrol-glucoside into resveratrol. Out of 13 bacterial strains tested, Lactobacillus kimchi JB301 could completely convert polydatin into resveratrol. The purified enzyme had an optimum temperature of 30-40°C and optimum pH of pH 5.0 against polydati...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2014.09.001
更新日期:2014-12-01 00:00:00
abstract::A reactive water-soluble polymer was synthesized by copolymerizing N-isopropylacrylamide and glycidyl acrylate. The reactive polymer could react with the amino groups of enzymes/proteins or other ligands to form an affinity polymer. As a model, the reactive polymer was allowed to react with paraaminobenzamidine, a str...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/0141-0229(90)90005-b
更新日期:1990-09-01 00:00:00
abstract::Global substitution of canonical amino acids (cAAs) with noncanonical (ncAAs) counterparts in proteins whose function is dependent on post-translational events such as cofactor binding is still a methodically challenging and difficult task as ncAA insertion generally interferes with the cofactor biosynthesis machinery...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2017.06.014
更新日期:2017-11-01 00:00:00
abstract::Water soluble ZnS quantum dots (QDs) modified by mercaptoacetic acid (MAA) were used to determinate proton concentration in aqueous solutions by fluorescence spectroscopic technique. The results showed that the fluorescence of the water-soluble QDs could be quenched by proton concentration and the fluorescence intensi...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2012.03.012
更新日期:2012-06-10 00:00:00
abstract::Different whole-cell immobilization methods and conditions were tested for the delta 1-dehydrogenation of 6-alpha-methyl-hydrocortisone-21-acetate with Arthrobacter simplex cells, in n-decane-1-ol and chloroform. Among the entrapment methods, polyurethane foams gave the best productivity of n-decane-1-ol, but could no...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/0141-0229(92)90036-n
更新日期:1992-08-01 00:00:00
abstract::In our previous study, we produced α-keto acids by using an L-amino acid deaminase PmiLAAD (wide-type) from Proteus mirabilis, however, the catalytic efficiency was low due to its low substrate affinity. In this study, protein engineering of PmiLAAD was performed to improve the α-keto acid production. PmiLAAD was engi...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2019.109393
更新日期:2020-01-01 00:00:00
abstract::Enzymatic production of cephalexin using immobilized penicillin G acylase was studied. The factors that affect the conversion of 7-amino-3-deacetoxycephalosporanic acid (7-ADCA) to cephalexin, such as pH; temperature; concentrations of 7-ADCA, D(-)-phenylglycine methyl ester (PGME), and immobilized penicillin G acylas...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/0141-0229(94)90095-7
更新日期:1994-08-01 00:00:00
abstract::Developing a strain with high docosahexaenoic acid (DHA) yield and stable fermenting-performance is an imperative way to improve DHA production using Aurantiochytrium sp., a microorganism with two fatty acid synthesis pathways: polyketide synthase (PKS) pathway and Type I fatty acid synthase (FAS) pathway. This study ...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2016.08.019
更新日期:2016-11-01 00:00:00
abstract::Kinetics of nitrification and denitrification of synthetic wastewater was investigated by using two reactors in series. An activated sludge unit was used for nitrification followed by a downflow biofilter for denitrification. Glucose solution was fed to the denitrification column to supply carbon source. Reactors were...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/s0141-0229(00)00145-9
更新日期:2000-07-01 00:00:00