On-line chemiluminescence assay using FIA and fiber optics for urinary and blood glucose.

abstract：:Malolactic fermentation (MLF) is a secondary bioconversion developed in some wines involving malic acid decarboxylation. The induction of MLF in wine by cultures of free and immobilized Oenococcus oeni cells was investigated. This work reports on the effect of surface charges in the immobilization material, a recently...

journal_title：Enzyme and microbial technology

authors： Maicas S,Pardo I,Ferrer S

更新日期：2001-03-08 00:00:00

abstract：:Sialic acid aldolases (E.C.4.1.3.3) catalyze the reversible aldol cleavage of N-acetyl-d-neuraminic acid (Neu5Ac) to from N-acetyl-d-mannosamine (ManNAc) and pyruvate. In this study, a sialic acid aldolase (PdNAL) from Peptoclostridium difficile NAP08 was expressed in Escherichia coli BL21 (DE3). This homotetrameric e...

journal_title：Enzyme and microbial technology

authors： Chen Q,Han L,Chen X,Cui Y,Feng J,Wu Q,Zhu D

更新日期：2016-10-01 00:00:00

abstract：:The xylanase R8 gene (xynR8) from uncultured rumen fungi was cloned and successfully expressed in Lactobacillus reuteri. A xylanase activity of 132.1 U/mL was found in the broth of L. reuteri R8, the transformant containing pNZ3004 vector with xynR8 gene insertion. Two distinct forms of recombinant xylanase with diffe...

journal_title：Enzyme and microbial technology

authors： Cheng HL,Hu CY,Lin SH,Wang JY,Liu JR,Chen YC

更新日期：2014-10-01 00:00:00

abstract：:Two novel glycosyl hydrolase family 5 (GH5) β-mannanases (AoMan5A and AoMan5B) were identified from Aspergillus oryzae RIB40 by genome mining. The AoMan5A contains a predicted family 1 carbohydrate binding module (CBM-1), located at its N-terminal. The AoMan5A, AoMan5B and truncated mutant AoMan5AΔCL (truncating the N...

journal_title：Enzyme and microbial technology

authors： Tang CD,Shi HL,Tang QH,Zhou JS,Yao LG,Jiao ZJ,Kan YC

更新日期：2016-11-01 00:00:00

abstract：:Magnetosomes are intracellular structures produced by magnetotactic bacteria and are magnetic nanoparticles surrounded by a lipid bilayer membrane. Magnetosomes reportedly possess intrinsic enzyme mimetic activity similar to that found in horseradish peroxidase (HRP) and can scavenge reactive oxygen species depending ...

journal_title：Enzyme and microbial technology

authors： Li K,Chen C,Chen C,Wang Y,Wei Z,Pan W,Song T

更新日期：2015-05-01 00:00:00

abstract：:'Candidatus Liberibacter asiaticus' ('Ca. L. asiaticus'), the suspected causative agent of citrus greening disease, is one of many phloem-restricted plant pathogens that have not been isolated and grown in an axenic culture. In this study, infected Asian citrus psyllids were used to prepare a host-free source of 'Ca. ...

journal_title：Enzyme and microbial technology

authors： Molki B,Call DR,Ha PT,Omsland A,Gang DR,Lindemann SR,Killiny N,Beyenal H

更新日期：2020-12-01 00:00:00

abstract：:We have designed an electrochemical DNA biosensor based on stem-loop structured probes for enzymatic detection of Pseudomonas aeruginosa 16S ribosomal RNA (rRNA) in composting degradation. The probe modified with a thiol at its 5' end and a biotin at its 3' end was immobilized on a gold electrode through self-assembly...

journal_title：Enzyme and microbial technology

authors： Liu C,Zeng GM,Tang L,Zhang Y,Li YP,Liu YY,Li Z,Wu MS,Luo J

更新日期：2011-08-10 00:00:00

abstract：:In the present work, NAD(+)-dependent formate dehydrogenase (FDH), encoded by fdh gene from Candida boidinii was successfully displayed on Escherichia coli cell surface using ice nucleation protein (INP) from Pseudomonas borealis DL7 as an anchoring protein. Localization of matlose binding protein (MBP)-INP-FDH fusion...

journal_title：Enzyme and microbial technology

authors： Liu A,Feng R,Liang B

更新日期：2016-09-01 00:00:00

abstract：:Phenethyl ester is an important contributors to food, drinks, cosmetics and perfumery. Phenethyl acetate was synthesized enzymatically from phenethyl alcohol with acyl donors catalyzed by Novozym 435 from Candida antarctica lipase B. Through acyl donor screening, acetic anhydride and vinyl acetate were found to be the...

journal_title：Enzyme and microbial technology

authors： Kim H,Park C

更新日期：2017-05-01 00:00:00

abstract：:Conventional lipase screening methods are mostly based on hydrolytic activity, which may not always be the best method to assess the enzyme activity, especially for evaluating synthetic activity. Here we developed a high throughput and visual method to screen clones with high synthetic activity and used it to assess l...

journal_title：Enzyme and microbial technology

authors： Zhang JH,Lin Y,Sun YF,Ye YR,Zheng SP,Han SY

更新日期：2012-05-10 00:00:00

abstract：:Two acidic β-glucosidases (βGI and βGII) from the brown rot fungus Fomitopsis palustris were purified to homogeneity by several chromatographic steps. βGI and βGII had molecular weights of 130 and 213 kDa, respectively, and exhibited optimum activity at pH 2.5 and 55°C. The K(m) values of βGI and βGII for p-nitropheny...

journal_title：Enzyme and microbial technology

authors： Okamoto K,Sugita Y,Nishikori N,Nitta Y,Yanase H

更新日期：2011-04-07 00:00:00

abstract：:Epoxide hydrolases (EHs) catalyse the conversion of epoxides into vicinal diols. These enzymes have extensive value in biocatalysis as they can generate enantiopure epoxides and diols which are important and versatile synthetic intermediates for the fine chemical and pharmaceutical industries. Despite these benefits, ...

journal_title：Enzyme and microbial technology

authors： Stojanovski G,Dobrijevic D,Hailes HC,Ward JM

更新日期：2020-09-01 00:00:00

abstract：:Cytochrome P450 enzyme preparations were obtained from phenobarbital-treated rats. On immobilization in Romicon PM 10 polysulfone anisotropic hollow fibers, 20-25% of the original activity remained. The immobilization procedure did not significantly alter the Km of the preparations when 7-ethoxycoumarin was the substr...

journal_title：Enzyme and microbial technology

authors： Fernandez-Salguero P,Gutierrez-Merino C,Bunch AW

更新日期：1993-02-01 00:00:00

abstract：:Various recombinant proteins can be produced by the yeast Saccharomyces cerevisiae cell factories; therefore, efficient recombinant protein production techniques are desirable. In this study, to establish an efficient recombinant protein production technique in S. cerevisiae, the secretory production of recombinant pr...

journal_title：Enzyme and microbial technology

authors： Sasaki Y,Mitsui R,Yamada R,Ogino H

更新日期：2019-02-01 00:00:00

abstract：:The objective of this study was to screen and identify α-amylase inhibitor peptides from Pinto bean. Five Pinto bean bioactive peptides were successfully identified: PPHMLP (P1), PLPWGAGF (P3), PPHMGGP (P6), PLPLHMLP (P7) and LSSLEMGSLGALFVCM (P9). Based on ELISA results, their promising optical density values were 1....

journal_title：Enzyme and microbial technology

authors： Ngoh YY,Lim TS,Gan CY

更新日期：2016-07-01 00:00:00

abstract：:Corynebacterium glutamicum is an important industrial organism for the production of a variety of biological commodities. We discovered a promoter encoded by the gene NCgl2319 in C. glutamicum, which could be induced by benzyl alcohol, could be used as an efficient tunable expression system. In initial attempts, this ...

journal_title：Enzyme and microbial technology

authors： Liu X,Zhao Z,Dong G,Li Y,Peng F,Liu C,Zhang F,Linhardt RJ,Yang Y,Bai Z

更新日期：2020-11-01 00:00:00

abstract：:The mechanical properties of TB/C3 hybridoma cells taken from a continuous culture were measured by micromanipulation. The culture conditions were constant except for the presence or absence of Pluronic F-68 in the medium. It was found that the mean bursting membrane tension and the mean elastic area compressibility m...

journal_title：Enzyme and microbial technology

authors： Zhang Z,al-Rubeai M,Thomas CR

更新日期：1992-12-01 00:00:00

abstract：:The full utilization of hemicellulose sugars (pentose and exose) present in lignocellulosic material, is required for an efficient bio-based fuels and chemicals production. Two recombinant thermophilic enzymes, an endo-1,4-β-mannanase from Dictyoglomus turgidum (DturCelB) and an α-galactosidase from Thermus thermophil...

journal_title：Enzyme and microbial technology

authors： Aulitto M,Fusco FA,Fiorentino G,Bartolucci S,Contursi P,Limauro D

更新日期：2018-04-01 00:00:00

abstract：:Global substitution of canonical amino acids (cAAs) with noncanonical (ncAAs) counterparts in proteins whose function is dependent on post-translational events such as cofactor binding is still a methodically challenging and difficult task as ncAA insertion generally interferes with the cofactor biosynthesis machinery...

journal_title：Enzyme and microbial technology

authors： Völler JS,Thi To TM,Biava H,Koksch B,Budisa N

更新日期：2017-11-01 00:00:00

abstract：:Zygosaccharomyces rouxii is an important yeast in the formation of flavor in soy sauce. In this study, we investigated the separate effects of exogenous threonine, cystathionine, and the branched-chain amino acids on the metabolism of Z. rouxii. The addition of these amino acids had significant effects on both Z. roux...

journal_title：Enzyme and microbial technology

authors： van der Sluis C,Wolken WA,Giuseppin ML,Tramper J,Wijffels RH

更新日期：2000-02-01 00:00:00

abstract：:Ergosterol, like cholesterol, has many beneficial physiological activities, and because it has no adverse clinical problem with cholesterol, it is a substance that can be used as a biomaterial in the food and cosmetics industry. However, ergosterol has low oil solubility and is easily crystallized, which is problemati...

journal_title：Enzyme and microbial technology

authors： Park SH,Kim HK

更新日期：2020-09-01 00:00:00

abstract：:This work presents the purification and characterization of an extracellular alpha-amylase (1,4-alpha-D-glucan glucanohydrolase, EC 3.2.1.1) produced by a new lactic acid bacterium: Lactobacillus manihotivorans able to produce L(+) lactic acid from starch. The molecular weight was found to be 135 kDa. The temperature ...

journal_title：Enzyme and microbial technology

authors： Aguilar G,Morlon-Guyot J,Trejo-Aguilar B,Guyot JP

更新日期：2000-09-01 00:00:00

abstract：:Photothermal therapy (PTT) is a minimally invasive and effective cancer treatment method and has a great potential for innovating the conventional chemotherapy approaches. Copper sulfide (CuS) exhibits photostability, low cost, and high absorption in near infrared region, and is recognized as an ideal candidate for PT...

journal_title：Enzyme and microbial technology

authors： Zhou NQ,Tian LJ,Wang YC,Li DB,Li PP,Zhang X,Yu HQ

更新日期：2016-12-01 00:00:00

abstract：:Using bioinformatic homology search tools, this study utilized sequence phylogeny, gene organization and conserved motifs to identify members of the family of O-methyltransferases from lignin-degrading fungus Phanerochaete chrysosporium. The heterologous expression and characterization of O-methyltransferases from P. ...

journal_title：Enzyme and microbial technology

authors： Thanh Mai Pham L,Kim YH

更新日期：2016-01-01 00:00:00

abstract：:Zymomonas mobilis genes encoding INVA and INVB were expressed in Pichia pastoris, under the control of the strong AOX1 promoter, and the recombinant enzymes were named INVAAOX1 and INVBAOX1. The expression levels of INVAAOX1 (1660 U/mg) and INVBAOX1 (1993 U/mg) in P. pastoris were 9- and 7-fold higher than those obser...

journal_title：Enzyme and microbial technology

authors： Pérez de los Santos AI,Cayetano-Cruz M,Gutiérrez-Antón M,Santiago-Hernández A,Plascencia-Espinosa M,Farrés A,Hidalgo-Lara ME

更新日期：2016-02-01 00:00:00

abstract：:This study aimed to improve the thermostability of a newly cloned tyrosinase from Streptomyces kathirae SC-1. The POPMuSiC algorithm was applied to predict the folding free energy change (ΔDG) of amino acid substitution. Site-directed mutagenesis was used to construct mutants (Q7K, G234P, and Q7K/G234P), and the mutan...

journal_title：Enzyme and microbial technology

authors： Guo J,Rao Z,Yang T,Man Z,Xu M,Zhang X,Yang ST

更新日期：2015-09-01 00:00:00

abstract：:Human interferon alpha 2b (IFN α2b) is a type I interferon exhibiting antiviral, anti-proliferative and immunomodulatory activities. The clinical outcome of the approved recombinant human IFN α2b drugs in the market suffers from short plasma half-life, rapid clearance and other side effects. Human IFN α2b expression i...

journal_title：Enzyme and microbial technology

authors： Katla S,Yoganand KNR,Hingane S,Ranjith Kumar CT,Anand B,Sivaprakasam S

更新日期：2019-09-01 00:00:00

abstract：:The effect of treatment with various intercalating dyes on the ability to produce antibiotics in Micromonospora rosaria and Micromonospora purpurea was studied. Treatment with acriflavine resulted in a high frequency loss of antibiotic productivity in both species. In M. rosaria, the loss of antibiotic-producing abili...

journal_title：Enzyme and microbial technology

authors： Kim KS,Pai HS,Lee SY,Ryu DD

更新日期：1990-08-01 00:00:00

abstract：:The different physical and chemical properties of lignin might have various effects on the enzymatic hydrolysis of lignocellulosic substrates. In this study, the influence of lignin on enzymatic digestibility of cellulose was assessed. Addition of 20% (4g/L) isolated enzymatic lignin (lignin 2 and 3) and kraft lignin ...

journal_title：Enzyme and microbial technology

authors： Zhang H,Wu S,Xie J

更新日期：2017-06-01 00:00:00

abstract：:Bacterial spores maintain metabolic dormancy and have high resistance to external pressure. Germination requires degradation of the spore cortex and the participation of germination-specific cortex-lytic enzymes (GSLEs). Previously reported GSLEs have been identified in bacteria and facilitate germination. In this stu...

journal_title：Enzyme and microbial technology

authors： Fu Y,Liang L,Deng S,Wu Y,Yuan Y,Gao M

更新日期：2020-12-01 00:00:00