Global substitution of hemeproteins with noncanonical amino acids in Escherichia coli with intact cofactor maturation machinery.

abstract：:An extracellular esterase from the actinomycetes Amycolatopsis orientalis was found by screening. It is capable of splitting the isomeric mixture (K/J) of (I, Scheme 1) into 7-amino-3-methoxymethyl-3-cephem-4-carboxylic acid, pivalic acid, and acetaldehyde with a high yield. The purified enzyme of 55.4 Kd by SDS-PAGE ...

journal_title：Enzyme and microbial technology

authors： Sauber K,Aretz W,Meiwes J,Wollmann T

更新日期：1996-07-01 00:00:00

abstract：:When dissolved oxygen (DO) was maintained at saturation level during batch fermentations of Streptomyces clavuligerus (NRRL 3585), the accumulation of the intermediate penicillin N was lowered while formation of the end product cephamycin C was increased relative to fermentations without DO control. The specific activ...

journal_title：Enzyme and microbial technology

authors： Rollins MJ,Jensen SE,Wolfe S,Westlake DW

更新日期：1990-01-01 00:00:00

abstract：:This study examined the potential of untreated and alkali-pretreated sugarcane bagasse (SCB) in cellulase, reducing sugar (RS) and fungal biomass production via solid state fermentation (SSF) using Pycnoporus sanguineus. The impact of the composition, structure and cellulase adsorption ability of SCB on the production...

journal_title：Enzyme and microbial technology

authors： Yoon LW,Ngoh GC,Chua AS

更新日期：2013-09-10 00:00:00

abstract：:A methyl parathion hydrolase (MPH) gene, bjmpd, was cloned from a newly isolated MP-degrading bacterial strain, Burkholderia jiangsuensis MP-1T and heterologously expressed in Escherichia coli BL21 (DE3). Although the amino acid sequence of the bjmpd-encoded enzyme, named BjMPH, differed from that of MPH from Pseudomo...

journal_title：Enzyme and microbial technology

authors： Liu XY,Chen FF,Li CX,Luo XJ,Chen Q,Bai YP,Xu JH

更新日期：2016-11-01 00:00:00

abstract：:Cystathionine γ-lyase (CGL) is a key enzyme in the methionine-cysteine cycle in all living organisms forming cysteine, α-ketobutyrate and ammonia via homocysteine and cystathionine intermediates. Although, human and plant CGLs have been extensively studied at the molecular and mechanistic levels, there has been little...

journal_title：Enzyme and microbial technology

authors： El-Sayed AS,Abdel-Azeim S,Ibrahim HM,Yassin MA,Abdel-Ghany SE,Esener S,Ali GS

更新日期：2015-12-01 00:00:00

abstract：:We generated a bifunctional enzyme chimera containing the xylanase and lichenase coupled with SpyTag between them. Meanwhile, we generated another chimera containing SpyCatcher and elastin-like polypeptides (ELPs). As ELPs could bond to the xylanase-lichenase chimera through SpyTag/SpyCatcher spontaneous reaction in m...

journal_title：Enzyme and microbial technology

authors： Lin Y,Jin W,Wang J,Cai Z,Wu S,Zhang G

更新日期：2018-08-01 00:00:00

abstract：:An intracellular alcohol oxidase (AOX) was isolated from the white-rot basidiomycete Phanerochaete chrysosporium (Pch), grown on l-lactate induction medium, and purified to electrophoretic homogeneity. The dimeric protein consisted of two identical 75kDa subunits. The open reading frame of 1,956bp resulted in a monome...

journal_title：Enzyme and microbial technology

authors： Linke D,Lehnert N,Nimtz M,Berger RG

更新日期：2014-07-01 00:00:00

abstract：:Various recombinant proteins can be produced by the yeast Saccharomyces cerevisiae cell factories; therefore, efficient recombinant protein production techniques are desirable. In this study, to establish an efficient recombinant protein production technique in S. cerevisiae, the secretory production of recombinant pr...

journal_title：Enzyme and microbial technology

authors： Sasaki Y,Mitsui R,Yamada R,Ogino H

更新日期：2019-02-01 00:00:00

abstract：:Chemical modification of lysine residues in two bacterial alpha-amylases, a mesophilic enzyme from Bacillus amyloliquefaciens (BAA) and a thermophilic enzyme from Bacillus licheniformis (BLA) was carried out using citraconic anhydride. 13 +/- 1 residues in BAA and 10 +/- 1 residues in BLA were found modified under def...

journal_title：Enzyme and microbial technology

authors： Khajeh K,Naderi-Manesh H,Ranjbar B,Moosavi-Movahedi A,Nemat-Gorgani M

更新日期：2001-04-05 00:00:00

abstract：:The lipases from Thermomyces lanuginosus and Pseudomonas cepacia have been immobilized on octyl and cyanogen bromide (CNBr) agarose beads. The immobilization on octyl-agarose is slowed with increasing ionic strength, while the immobilization on CNBr is not significantly affected by the ionic strength. The inhibition o...

journal_title：Enzyme and microbial technology

authors： Manoel EA,Dos Santos JC,Freire DM,Rueda N,Fernandez-Lafuente R

更新日期：2015-04-01 00:00:00

abstract：:This work presents the purification and characterization of an extracellular alpha-amylase (1,4-alpha-D-glucan glucanohydrolase, EC 3.2.1.1) produced by a new lactic acid bacterium: Lactobacillus manihotivorans able to produce L(+) lactic acid from starch. The molecular weight was found to be 135 kDa. The temperature ...

journal_title：Enzyme and microbial technology

authors： Aguilar G,Morlon-Guyot J,Trejo-Aguilar B,Guyot JP

更新日期：2000-09-01 00:00:00

abstract：:The effect of treatment with various intercalating dyes on the ability to produce antibiotics in Micromonospora rosaria and Micromonospora purpurea was studied. Treatment with acriflavine resulted in a high frequency loss of antibiotic productivity in both species. In M. rosaria, the loss of antibiotic-producing abili...

journal_title：Enzyme and microbial technology

authors： Kim KS,Pai HS,Lee SY,Ryu DD

更新日期：1990-08-01 00:00:00

abstract：:Advantages in maltose fermentation at extremely low temperatures (5-10°C) using an alcohol resistant and cryotolerant yeast strain (Saccharomyces cerevisiae AXAZ-1) immobilized on porous cellulosic material (or tubular cellulose, TC), produced by delignification of wood sawdust, are reported. Pure maltose and glucose ...

journal_title：Enzyme and microbial technology

authors： Ganatsios V,Koutinas AA,Bekatorou A,Kanellaki M,Nigam P

更新日期：2014-11-01 00:00:00

abstract：:Recently, we reported a highly sensitive immunoassay using Escherichia coli cells with autodisplayed Z-domains. In this work, E. coli cells with autodisplayed Z-domains were applied to the flow-cytometry-based simultaneous detection of multiple analytes. The E. coli cells were doubly transfected to express a fluoresce...

journal_title：Enzyme and microbial technology

authors： Park M,Bong JH,Yoo G,Jose J,Kang MJ,Pyun JC

更新日期：2013-08-15 00:00:00

abstract：:Phytate is the major storage form of phosphorus in plants. It is present in cereals and raw materials of vegetable origin used in animal and human diets. However, non-ruminant animals have little phytase activity in their guts and, therefore, cannot digest phytate. As a result, almost all dietary phytate is discharged...

journal_title：Enzyme and microbial technology

authors： Xie Z,Fong WP,Tsang PW

更新日期：2020-06-01 00:00:00

abstract：:We here describe a unique β-D-glucosidase (BGL; Blon_0625) derived from Bifidobacterium longum subsp. infantis ATCC 15697. The Blon_0625 gene was expressed by recombinant Escherichia coli. Purified recombinant Blon_0625 retains hydrolyzing activity against both p-nitrophenyl-β-D-glucopyranoside (pNPG; 17.3±0.24Umg(-1)...

journal_title：Enzyme and microbial technology

authors： Matsumoto T,Shimada S,Hata Y,Tanaka T,Kondo A

更新日期：2015-01-01 00:00:00

abstract：:The kinetic and thermodynamic parameters of wheat β-amylase (WBA) were characterized and various additives were evaluated for enhancing its activity and thermostability. WBA activity was examined by neocuproine method using soluble starch as substrate. The Michaelis constant (K(m)) and molecular activity (k(cat)) were...

journal_title：Enzyme and microbial technology

authors： Daba T,Kojima K,Inouye K

更新日期：2012-10-10 00:00:00

abstract：:The effect of hexoses (glucose and galactose) addition to the feed xylose mineral medium of Debaryomyces hansenii chemostat cultures grown at a constant dilution rate of 0.055 h(-1) was studied. Xylitol was the major product detected amongst all tested conditions. The maximal values for xylitol yield and volumetric pr...

journal_title：Enzyme and microbial technology

authors： Tavares JM,Duarte LC,Amaral-Collaço MT,Gírio FM

更新日期：2000-06-01 00:00:00

abstract：:Spodoptera frugiperda insect cells (IPLB-Sf21-AE) (Sf21), infected with baculovirus expression vectors during their exponential growth phase, are commonly used to produce a variety of heterologous recombinant proteins. In the present study the culture conditions of these insect cells were studied to establish high-den...

journal_title：Enzyme and microbial technology

authors： Deutschmann SM,Jäger V

更新日期：1994-06-01 00:00:00

abstract：:Industrial use of Novozym 435 in synthesis of structured lipids and biodiesel via alcoholysis is limited by mass transfer effects of the glycerides through immobilized enzymes and its low operational stability under operation conditions. To better understand this, differently modified Novozym 435 preparations, differi...

journal_title：Enzyme and microbial technology

authors： Villalba M,Verdasco-Martín CM,Dos Santos JC,Fernandez-Lafuente R,Otero C

更新日期：2016-08-01 00:00:00

abstract：:The methyl ester of 2-benzoxazolon-3-yl-acetic acid was used as an acyl donor in the penicillin amidase-catalysed transfer reaction to 7-aminodesacetoxycephalosporanic acid. The synthesis of 7-(2-benzoxazolon-3-yl-acetamido)-desacetoxycephalosporanic acid was carried out as a kinetically controlled reaction. A charact...

journal_title：Enzyme and microbial technology

authors： Stambolieva N,Mincheva Z,Galunsky B,Kalcheva V

更新日期：1992-06-01 00:00:00

abstract：:The yeast Pichia pastoris is a widely used host for recombinant protein expression, and has recently been engineered for whole-cell biocatalysis. The inducible P(AOX) and constitutive P(GAP) promoters are commonly employed. In this study, the S-adenosyl-L-methionine (SAM) biosynthesis and degradation efficiency of two...

journal_title：Enzyme and microbial technology

authors： Hu X,Chu J,Zhang S,Zhuang Y

更新日期：2014-02-05 00:00:00

abstract：:Horseradish peroxidase (HRP) was successfully purified from horseradish roots by a two-stage reverse-micellar extraction from the dialyzed aqueous extract. The anionic surfactant AOT dissolved in isooctane was used to produce the reverse-micellar phases. The narrow pH range at which HRP solubilization occurred was exp...

journal_title：Enzyme and microbial technology

authors： Regalado C,Asenjo JA,Pyle DL

更新日期：1996-04-01 00:00:00

abstract：:Turanose is a natural isomer of sucrose. It is an emerging functional sweetener of the next generation. Turanose is catalytically synthesized from the sucrose biomass by employing amylosucrase enzyme. In this study, a novel gene encoding amylosucrase (Asmet) has been identified from the metagenome of a thermal aquatic...

journal_title：Enzyme and microbial technology

authors： Agarwal N,Narnoliya LK,Singh SP

更新日期：2019-12-01 00:00:00

abstract：:D-glucuronic acid (GlcUA) is an important intermediate with numerous applications in the food, cosmetics, and pharmaceutical industries. Its biological production routes which employ myo-inositol oxygenase (MIOX) as the key enzyme are attractive. In this study, five diverse MIOX-encoding genes, from Cryptococcus neofo...

journal_title：Enzyme and microbial technology

authors： Teng F,You R,Hu M,Liu W,Wang L,Tao Y

更新日期：2019-08-01 00:00:00

abstract：:The primary aim of this study was to decipher the catalytic functions of the NHase with wide substrate spectra from Rhodococcus ruber CGMCC3090 by computer modeling and substrate docking. 3D structure model of the enzyme was built by computer modeling to obtain the optimal structure. The larger binding site cavity (55...

journal_title：Enzyme and microbial technology

authors： Wang S,Dai Y,Wang J,Shen Y,Zhai Y,Zheng H,Wang M

更新日期：2013-02-05 00:00:00

abstract：:The fatty acid methyl esters and fatty acid ethyl esters are known as biodiesels which are considered to be renewable, nontoxic and biodegradable biofuels. However, the conventional biodiesels show a high crystallization temperature which is one of the most critical obstacles against the widespread biodiesel usage. Th...

journal_title：Enzyme and microbial technology

authors： Pan H,Zhang L,Li X,Guo D

更新日期：2017-07-01 00:00:00

abstract：:A reactive water-soluble polymer was synthesized by copolymerizing N-isopropylacrylamide and glycidyl acrylate. The reactive polymer could react with the amino groups of enzymes/proteins or other ligands to form an affinity polymer. As a model, the reactive polymer was allowed to react with paraaminobenzamidine, a str...

journal_title：Enzyme and microbial technology

authors： Nguyen AL,Luong JH

更新日期：1990-09-01 00:00:00

abstract：:Enzymatic production of cephalexin using immobilized penicillin G acylase was studied. The factors that affect the conversion of 7-amino-3-deacetoxycephalosporanic acid (7-ADCA) to cephalexin, such as pH; temperature; concentrations of 7-ADCA, D(-)-phenylglycine methyl ester (PGME), and immobilized penicillin G acylas...

journal_title：Enzyme and microbial technology

authors： Maladkar NK

更新日期：1994-08-01 00:00:00

abstract：:The heat and mass transfer have been proved to be the important factors in air pressure pulsation for cellulase production. However, as process of enzyme secretion, the cellulase formation has not been studied in the view of microorganism metabolism and metabolic key enzyme activity under air pressure pulsation condit...

journal_title：Enzyme and microbial technology

authors： Chen H,Shao M,Li H

更新日期：2014-03-05 00:00:00