Biosynthesis of the fatty acid isopropyl esters by engineered Escherichia coli.


:The fatty acid methyl esters and fatty acid ethyl esters are known as biodiesels which are considered to be renewable, nontoxic and biodegradable biofuels. However, the conventional biodiesels show a high crystallization temperature which is one of the most critical obstacles against the widespread biodiesel usage. The high crystallization temperature of biodiesel can be reduced by replacing the methyl or ethyl ester with an isopropyl moiety. Here we report on a strategy to establish biosynthesis of the fatty acid isopropyl esters(FAIPEs) from the simple substrate glucose in Escherichia coli with heterologous coexpression of atoB encoded acetyl-CoA acetyltransferase and atoAD encode acetoacetyl-CoA transferase from E. coli, ADC encode acetoacetate decarboxylase from Clostridium acetobutylicum, ADH encoded NADP-dependent alcohol dehydrogenase from Clostridium beijerinckii, 'TesA encoded a truncated fatty acyl-ACP thioesterase and FadD encoded fatty acyl-CoA synthetase from E. coli, and the WS/DGAT encoded acyltransferase from Acinetobacter baylyi strain ADP1. It was found that the yield of FAIPEs was up to 203.4mg/L and accounted for around 6.4% (wt/wt) of the dry cell weight. Our results indicates that it is a feasible strategy to improve the yield of FAIPEs by increasing fatty acyl-CoA availability in biosynthetic pathway and exhibit a promising method for production of biodiesels with good low-temperature flow properties.


Enzyme Microb Technol


Pan H,Zhang L,Li X,Guo D




Has Abstract


2017-07-01 00:00:00












  • Whole-cell bio-oxidation of n-dodecane using the alkane hydroxylase system of P. putida GPo1 expressed in E. coli.

    abstract::The alkane-1-monoxygenase (alkB) complex of Pseudomonas putida GPo1 has been extensively studied in the past and shown to be capable of oxidising aliphatic C(5)-C(12) alkanes to primary alcohols both in the wild-type organism by growth on C(5)-C(12) alkanes as sole carbon source and in vitro. Despite this, successful ...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Grant C,Woodley JM,Baganz F

    更新日期:2011-05-06 00:00:00

  • Immobilization of lipases on hydrophobic supports involves the open form of the enzyme.

    abstract::The lipases from Thermomyces lanuginosus and Pseudomonas cepacia have been immobilized on octyl and cyanogen bromide (CNBr) agarose beads. The immobilization on octyl-agarose is slowed with increasing ionic strength, while the immobilization on CNBr is not significantly affected by the ionic strength. The inhibition o...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Manoel EA,Dos Santos JC,Freire DM,Rueda N,Fernandez-Lafuente R

    更新日期:2015-04-01 00:00:00

  • Modulatory effect of low-shear modeled microgravity on stress resistance, membrane lipid composition, virulence, and relevant gene expression in the food-borne pathogen Listeria monocytogenes.

    abstract::The present study investigated the influence of low-shear modeled microgravity (LSMMG) conditions on Listeria monocytogenes stress response (heat, cold, and acid), membrane fatty acid composition, and virulence potential as well as stress-/virulence-associated gene expression. The results showed that LSMMG-cultivated ...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Sheet S,Yesupatham S,Ghosh K,Choi MS,Shim KS,Lee YS

    更新日期:2020-02-01 00:00:00

  • The application of foaming for the recovery of Surfactin from B. subtilis ATCC 21332 cultures.

    abstract::Foaming, a proficient method for the recovery of surface active solutes from dilute solutions, was successfully applied for the concentration of the lipopeptide biosurfactant Surfactin from B. subtilis ATCC 21332 cell culture broths. Foaming was only partially successful in concentrating Surfactin when applied as a se...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Davis DA,Lynch HC,Varley J

    更新日期:2001-03-08 00:00:00

  • UV-Vis detection of hydrogen peroxide using horseradish peroxidase/copper phosphate hybrid nanoflowers.

    abstract::A colorimetric platform based on enzyme-inorganic hybrid nanoflowers was used for ultraviolet-visible detection of hydrogen peroxide (H2O2). The hybrid nanoflowers were prepared via incubation at room temperature using horseradish peroxidase (HRP) and copper phosphate (Cu3(PO4)2) as the organic and inorganic component...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Yang C,Zhang M,Wang W,Wang Y,Tang J

    更新日期:2020-10-01 00:00:00

  • Effects of non-ionic surfactants on the interactions between cellulases and tannic acid: a model system for cellulase-poly-phenol interactions.

    abstract::Addition of non-ionic surfactants (NIS) is known to accelerate enzymatic lignocellulose hydrolysis. The mechanism behind this accelerating effect is still not elucidated but has been hypothesized to originate from favorable NIS-lignin interactions which alleviate non-productive adsorption of cellulases to lignin. In t...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Olsen SN,Bohlin C,Murphy L,Borch K,McFarland KC,Sweeny MD,Westh P

    更新日期:2011-09-10 00:00:00

  • Exploring Trichoderma and Aspergillus secretomes: Proteomics approaches for the identification of enzymes of biotechnological interest.

    abstract::Filamentous fungal secretomes comprise highly dynamic sets of proteins, including multiple carbohydrate active enzymes (CAZymes) which are able to hydrolyze plant biomass polysaccharides into products of biotechnological interest such as fermentable sugars. In recent years, proteomics has been used to identify and qua...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章,评审


    authors: Cologna NMD,Gómez-Mendoza DP,Zanoelo FF,Giannesi GC,Guimarães NCA,Moreira LRS,Filho EXF,Ricart CAO

    更新日期:2018-02-01 00:00:00

  • On-line chemiluminescence assay using FIA and fiber optics for urinary and blood glucose.

    abstract::A chemiluminescence fiber optic system coupled to flow injection analysis (FIA) and ion exchange chromatography has been developed for determining glucose in blood and urine. Immobilized glucose oxidase acted on beta-D-glucose to produce hydrogen peroxide, which was then reacted with luminol in the presence of ferricy...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Cattaneo MV,Luong JH

    更新日期:1993-05-01 00:00:00

  • Sucrose hydrolysis by thermostable immobilized inulinases from aspergillus ficuum.

    abstract::The possibility of using thermostable inulinases from Aspergillus ficuum in place of invertase for sucrose hydrolysis was explored. The commercial inulinases preparation was immobilized onto porous glass beads by covalent coupling using activation by a silane reagent and glutaraldehyde before adding the enzyme. The im...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Ettalibi M,Baratti JC

    更新日期:2001-05-07 00:00:00

  • Direct cellobiose production from cellulose using sextuple beta-glucosidase gene deletion Neurospora crassa mutants.

    abstract::Direct cellobiose production from cellulose by a genetically modified fungus-Neurospora crassa, was explored in this study. A library of N. crassa sextuple beta-glucosidase (bgl) gene deletion strains was constructed. Various concentrations of cellobiose were detected in the culture broth of the N. crassa sextuple bet...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Wu W,Hildebrand A,Kasuga T,Xiong X,Fan Z

    更新日期:2013-03-05 00:00:00

  • The kinetics of affinity-mediated cell-surface attachment.

    abstract::Data and a semi-empirical model are presented that describe the affinity interaction of yeast cells with a Concanavalin A derivatised surface. The model uses 3 parameters to describe the time course of cell attachment from a flowing suspension of yeast cells, over a range of flow rates, and gives an effective global f...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Ming F,Eisenthal R,Whish WJ,Hubble J

    更新日期:2000-02-01 00:00:00

  • A bifunctional beta-xylosidase-xylose isomerase from Streptomyces sp. EC 10.

    abstract::beta-Xylosidase (1,4-beta-D-xylan xylohydrolase EC and xylose isomerase (D-xylose ketol-isomerase EC produced by Streptomyces sp. strain EC 10, were cell-bound enzymes induced by xylan, straw, and xylose. Enzyme production was subjected to a form of carbon catabolite repression by glycerol. beta-Xyl...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Belfaquih N,Penninckx MJ

    更新日期:2000-07-01 00:00:00

  • An efficient in vitro refolding of recombinant bacterial laccase in Escherichia coli.

    abstract::Laccases (benzenediol oxygen oxidoreductases, EC are important multicopper enzymes that are used in many biotechnological processes. A recombinant form of laccase from Bacillus sp. HR03 was overexpressed in Escherichia coli BL-21(DE3). Inclusion body (IB) formation happens quite often during recombinant prot...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Mollania N,Khajeh K,Ranjbar B,Rashno F,Akbari N,Fathi-Roudsari M

    更新日期:2013-05-10 00:00:00

  • Increase in the thermostability of GH11 xylanase XynJ from Bacillus sp. strain 41M-1 using site saturation mutagenesis.

    abstract::GH11 xylanase XynJ from Bacillus sp. strain 41M-1 has a β-jellyroll fold composed of eight β strands with a deep active-site cleft. We hypothesized that the thermostability of XynJ will increase if the flexibility of the β strands in the jellyroll structure is decreased without impairing activity. To verify this hypot...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Takita T,Nakatani K,Katano Y,Suzuki M,Kojima K,Saka N,Mikami B,Yatsunami R,Nakamura S,Yasukawa K

    更新日期:2019-11-01 00:00:00

  • Efficient enzymatic synthesis of α-keto acids by redesigned substrate-binding pocket of the l-amino acid deaminase (PmiLAAD).

    abstract::In our previous study, we produced α-keto acids by using an L-amino acid deaminase PmiLAAD (wide-type) from Proteus mirabilis, however, the catalytic efficiency was low due to its low substrate affinity. In this study, protein engineering of PmiLAAD was performed to improve the α-keto acid production. PmiLAAD was engi...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Wu L,Guo X,Wu G,Liu P,Liu Z

    更新日期:2020-01-01 00:00:00

  • ZnS quantum dots as pH probes for study of enzyme reaction kinetics.

    abstract::Water soluble ZnS quantum dots (QDs) modified by mercaptoacetic acid (MAA) were used to determinate proton concentration in aqueous solutions by fluorescence spectroscopic technique. The results showed that the fluorescence of the water-soluble QDs could be quenched by proton concentration and the fluorescence intensi...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Wu D,Chen Z

    更新日期:2012-06-10 00:00:00

  • Progress in using threonine aldolases for preparative synthesis.

    abstract::Three threonine aldolases (TAs) were cloned and overexpressed in Escherichia coli (Aeromonas jandaeil-allo-threonine aldolase, E. colil-threonine aldolase and Thermotoga maritimal-allo-threonine aldolase). A Design of Experiments strategy was used to identify optimal reaction conditions for each enzyme. These conditio...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Beaudoin SF,Hanna MP,Ghiviriga I,Stewart JD

    更新日期:2018-12-01 00:00:00

  • Intracellular synthesis of gold nanoparticles with antioxidant activity by probiotic Lactobacillus kimchicus DCY51T isolated from Korean kimchi.

    abstract::A straightforward synthesis of gold nanoparticles (AuNps) is achieved by novel probiotic Lactobacillus kimchicus DCY51T isolated from Korean kimchi via an intracellular membrane-bound mechanism. The bioreduction of HAuCl4 into AuNps was verified by ultraviolet-visible spectrophotometry at ∼540nm. AuNps were spherical ...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Markus J,Mathiyalagan R,Kim YJ,Abbai R,Singh P,Ahn S,Perez ZEJ,Hurh J,Yang DC

    更新日期:2016-12-01 00:00:00

  • Stability of a recombinant shuttle plasmid in Bacillus subtilis and Escherichia coli.

    abstract::The recombinant shuttle plasmid pCPPS-31, which confers carboxymethylcellulase (CMCase) production and neomycin resistance (Ner), was segregationally but not structurally unstable in both B. subtilis (50-60%) and Escherichia coli (80-90%) when grown in serial batch cultures for 72 h diluting 1:1,000 with fresh medium ...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Vyas VV,Gupta S,Sharma P

    更新日期:1994-03-01 00:00:00

  • Estimation of the kinetic constants and elucidation of trends in growth and erythromycin production in batch and continuous cultures of Saccharopolyspora erythraea using curve-fitting techniques.

    abstract::The kinetics of erythromycin production were dependent on the identity of the growth rate-limiting nutrient during batch cultures of Saccharopolyspora erythraea. Semilogarithmic linear regression provided a single estimate of growth rate during the exponential phase, but partial cubic spline curve fit derivatives prov...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: McDermott JF,Lethbridge G,Bushell ME

    更新日期:1993-08-01 00:00:00

  • Fast, affordable and eco-friendly enzyme kinetic method for the assay of α-ketoglutaric acid in medical product and sports supplements.

    abstract::A novel kinetic method was developed for the quantitation of α-ketoglutaric acid (AKG) in cardioplegic solution and athletic supplements. The assay relied on an enzymatic transamination of AKG and d-4-hydroxyphenylglycine to form 4-hydroxybenzoylformic acid and l-glutamic acid using d-phenylglycine aminotransferase. S...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Rojanarata T,Laiwattanapaisal W,Ngawhirunpat T,Opanasopit P,Worrawethanakul K,Chantadee T,Fuangwattana T

    更新日期:2018-09-01 00:00:00

  • Utilization of distillery wastewater for hydrogen production in one-stage and two-stage processes involving photofermentation.

    abstract::In this study, distillery wastewater was treated by dark fermentation or photofermentation alone, and by sequential dark and photofermentation processes using anaerobic saccharolytic consortium and purple nonsulfur bacteria. Combination of dark and photofermentation resulted in the maximal H2 yield of 17.6L/L of disti...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Laurinavichene T,Tekucheva D,Laurinavichius K,Tsygankov A

    更新日期:2018-03-01 00:00:00

  • Effect of intense pulsed light on the deactivation of lipase: Enzyme-deactivation kinetics and tertiary structural changes by fragmentation.

    abstract::The effect of intense pulsed light (IPL) irradiation on Chromobacterium viscosum lipase was investigated with a primary focus on catalytic activity and molecular structure. During IPL irradiation, lipase activity decreased significantly with increasing pulse fluence (Fp) and exposure time (te). IPL-induced deactivatio...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Jeon MS,Park KM,Yu H,Park JY,Chang PS

    更新日期:2019-05-01 00:00:00

  • Enhanced detection of RNA by MMLV reverse transcriptase coupled with thermostable DNA polymerase and DNA/RNA helicase.

    abstract::Detection of mRNA is a valuable method for monitoring the specific gene expression. In this study, we devised a novel cDNA synthesis method using three enzymes, the genetically engineered thermostable variant of reverse transcriptase (RT), MM4 (E286R/E302K/L435R/D524A) from Moloney murine leukemia virus (MMLV), the ge...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Okano H,Katano Y,Baba M,Fujiwara A,Hidese R,Fujiwara S,Yanagihara I,Hayashi T,Kojima K,Takita T,Yasukawa K

    更新日期:2017-01-01 00:00:00

  • Effects of gas periodic stimulation on key enzyme activity in gas double-dynamic solid state fermentation (GDD-SSF).

    abstract::The heat and mass transfer have been proved to be the important factors in air pressure pulsation for cellulase production. However, as process of enzyme secretion, the cellulase formation has not been studied in the view of microorganism metabolism and metabolic key enzyme activity under air pressure pulsation condit...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Chen H,Shao M,Li H

    更新日期:2014-03-05 00:00:00

  • Enzyme reaction engineering: design of peptide synthesis by stabilized trypsin.

    abstract::By using very active and very stable trypsin agarose derivatives, we have optimized the design of the synthesis of a model dipeptide, benzoylarginine leucinamide, by two different strategies: (i) kinetically controlled synthesis (KCS), by using benzoyl arginine ethyl ester and leucinamide as substrates, and (ii) therm...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Blanco RM,Alvaro G,Guisán JM

    更新日期:1991-07-01 00:00:00

  • Porous biochar/chitosan composites for high performance cellulase immobilization by glutaraldehyde.

    abstract::In this work, porous biochar derived from sugarcane bagasse was prepared and then coated with different amounts of chitosan (C@CS) for cellulase immobilization. Cellulase was covalently immobilized on the support by using glutaraldehyde as a linker. The chemical characteristics and morphology of the samples were deter...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Mo H,Qiu J,Yang C,Zang L,Sakai E,Chen J

    更新日期:2020-08-01 00:00:00

  • The effect of gas double-dynamic on mass distribution in solid-state fermentation.

    abstract::The mass distribution regularity in substrate of solid-state fermentation (SSF) has rarely been reported due to the heterogeneity of solid medium and the lack of suitable instrument and method, which limited the comprehensive analysis and enhancement of the SSF performance. In this work, the distributions of water, bi...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Chen HZ,Zhao ZM,Li HQ

    更新日期:2014-05-10 00:00:00

  • Chemical modification of lysine residues in Bacillus alpha-amylases: effect on activity and stability.

    abstract::Chemical modification of lysine residues in two bacterial alpha-amylases, a mesophilic enzyme from Bacillus amyloliquefaciens (BAA) and a thermophilic enzyme from Bacillus licheniformis (BLA) was carried out using citraconic anhydride. 13 +/- 1 residues in BAA and 10 +/- 1 residues in BLA were found modified under def...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Khajeh K,Naderi-Manesh H,Ranjbar B,Moosavi-Movahedi A,Nemat-Gorgani M

    更新日期:2001-04-05 00:00:00

  • Cellulase-lignin interactions-the role of carbohydrate-binding module and pH in non-productive binding.

    abstract::Non-productive cellulase adsorption onto lignin is a major inhibitory mechanism preventing enzymatic hydrolysis of lignocellulosic feedstocks. Therefore, understanding of enzyme-lignin interactions is essential for the development of enzyme mixtures and processes for lignocellulose hydrolysis. We have studied cellulas...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Rahikainen JL,Evans JD,Mikander S,Kalliola A,Puranen T,Tamminen T,Marjamaa K,Kruus K

    更新日期:2013-10-10 00:00:00