Abstract:
:The fatty acid methyl esters and fatty acid ethyl esters are known as biodiesels which are considered to be renewable, nontoxic and biodegradable biofuels. However, the conventional biodiesels show a high crystallization temperature which is one of the most critical obstacles against the widespread biodiesel usage. The high crystallization temperature of biodiesel can be reduced by replacing the methyl or ethyl ester with an isopropyl moiety. Here we report on a strategy to establish biosynthesis of the fatty acid isopropyl esters(FAIPEs) from the simple substrate glucose in Escherichia coli with heterologous coexpression of atoB encoded acetyl-CoA acetyltransferase and atoAD encode acetoacetyl-CoA transferase from E. coli, ADC encode acetoacetate decarboxylase from Clostridium acetobutylicum, ADH encoded NADP-dependent alcohol dehydrogenase from Clostridium beijerinckii, 'TesA encoded a truncated fatty acyl-ACP thioesterase and FadD encoded fatty acyl-CoA synthetase from E. coli, and the WS/DGAT encoded acyltransferase from Acinetobacter baylyi strain ADP1. It was found that the yield of FAIPEs was up to 203.4mg/L and accounted for around 6.4% (wt/wt) of the dry cell weight. Our results indicates that it is a feasible strategy to improve the yield of FAIPEs by increasing fatty acyl-CoA availability in biosynthetic pathway and exhibit a promising method for production of biodiesels with good low-temperature flow properties.
journal_name
Enzyme Microb Technoljournal_title
Enzyme and microbial technologyauthors
Pan H,Zhang L,Li X,Guo Ddoi
10.1016/j.enzmictec.2017.03.012subject
Has Abstractpub_date
2017-07-01 00:00:00pages
49-52eissn
0141-0229issn
1879-0909pii
S0141-0229(17)30056-Xjournal_volume
102pub_type
杂志文章abstract::We report a new colorimetric assay to quantify endo-polygalacturonase activity, which hydrolyzes polygalacturonic acid to produce smaller chains of galacturonate. Some of the reported polygalacturonase assays measure the activity by detecting the appearance of reducing ends such as the Somogyi-Nelson method. As a resu...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2010.04.004
更新日期:2011-02-08 00:00:00
abstract::Flocculent yeast Saccharomyces cerevisiae YF234 (MATa ura3-52 trp1Delta2 his ade 2-1 can1-100 sta1 FLO8) cells overexpressing glyoxalase I and having strong flocculation ability were permeabilized with isopropyl alcohol and ethanol under various conditions. The treatment with 40% isopropyl alcohol significantly improv...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/s0141-0229(00)00304-5
更新日期:2000-12-01 00:00:00
abstract::This study aimed to develop viable enzymes for bioconversion of resveratrol-glucoside into resveratrol. Out of 13 bacterial strains tested, Lactobacillus kimchi JB301 could completely convert polydatin into resveratrol. The purified enzyme had an optimum temperature of 30-40°C and optimum pH of pH 5.0 against polydati...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2014.09.001
更新日期:2014-12-01 00:00:00
abstract::Phytate is the major storage form of phosphorus in plants. It is present in cereals and raw materials of vegetable origin used in animal and human diets. However, non-ruminant animals have little phytase activity in their guts and, therefore, cannot digest phytate. As a result, almost all dietary phytate is discharged...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2020.109533
更新日期:2020-06-01 00:00:00
abstract::Effects of pulse and step changes in major metabolite concentrations such as glucose, glutamine, lactic acid, and ammonium on behavior of T-cell hybridomas in continuous culture were investigated in order to develop a strategy to maximize specific rate of lymphokine formation. Step increases in glucose, lactic acid, a...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/0141-0229(90)90007-d
更新日期:1990-09-01 00:00:00
abstract::Phenethyl ester is an important contributors to food, drinks, cosmetics and perfumery. Phenethyl acetate was synthesized enzymatically from phenethyl alcohol with acyl donors catalyzed by Novozym 435 from Candida antarctica lipase B. Through acyl donor screening, acetic anhydride and vinyl acetate were found to be the...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2017.02.004
更新日期:2017-05-01 00:00:00
abstract::Human interferon alpha 2b (IFN α2b) is a type I interferon exhibiting antiviral, anti-proliferative and immunomodulatory activities. The clinical outcome of the approved recombinant human IFN α2b drugs in the market suffers from short plasma half-life, rapid clearance and other side effects. Human IFN α2b expression i...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2019.05.007
更新日期:2019-09-01 00:00:00
abstract::An analysis developed by Westgate et al. for the digestible energy of edible and inedible biomass, including hydrolysis and fermentation, is reexamined with state-of-the-art values for the harvest index of hydroponic crops. ...
journal_title:Enzyme and microbial technology
pub_type: 评论,杂志文章
doi:10.1016/0141-0229(93)90104-a
更新日期:1993-10-01 00:00:00
abstract::beta-Xylosidase (1,4-beta-D-xylan xylohydrolase EC 3.2.1.37) and xylose isomerase (D-xylose ketol-isomerase EC 5.3.1.5) produced by Streptomyces sp. strain EC 10, were cell-bound enzymes induced by xylan, straw, and xylose. Enzyme production was subjected to a form of carbon catabolite repression by glycerol. beta-Xyl...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/s0141-0229(00)00182-4
更新日期:2000-07-01 00:00:00
abstract::Enzymatic synthesis of cephalothin from 7-aminocephalosporanic acid (7-ACA) and amide derivatives of 2-thienylacetic acid (2-TA) using penicillin G acylase (pen G acylase) was studied. Two amide derivatives of 2-TA namely 2-thienylacetamide (2-TAA) and 2-thienylacetohydroxamic acid (2-TAH) were used in this study. The...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/s0141-0229(99)00153-2
更新日期:2000-02-01 00:00:00
abstract::Marine microbes have gained significant attention as potential biofactories for broad spectrum bioactive compounds. In the recent years, bioactive biosurfactants have warranted renewed interest from both environmental and clinical sectors as anti-biofilm agents due to their excellent properties of dispersing microbial...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2018.09.008
更新日期:2019-01-01 00:00:00
abstract::The heat and mass transfer have been proved to be the important factors in air pressure pulsation for cellulase production. However, as process of enzyme secretion, the cellulase formation has not been studied in the view of microorganism metabolism and metabolic key enzyme activity under air pressure pulsation condit...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2013.12.015
更新日期:2014-03-05 00:00:00
abstract::In this work, a biosensor using a glassy carbon electrode modified with gold nanoparticles (AuNPs) and tyrosinase (Tyr) within a dihexadecylphosphate film is proposed. Cystamine and glutaraldehyde crosslinking agents were used as a support for Tyr immobilization. The proposed biosensor was characterized by scanning el...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2015.12.004
更新日期:2016-03-01 00:00:00
abstract::The effect of hexoses (glucose and galactose) addition to the feed xylose mineral medium of Debaryomyces hansenii chemostat cultures grown at a constant dilution rate of 0.055 h(-1) was studied. Xylitol was the major product detected amongst all tested conditions. The maximal values for xylitol yield and volumetric pr...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/s0141-0229(00)00166-6
更新日期:2000-06-01 00:00:00
abstract::'Candidatus Liberibacter asiaticus' ('Ca. L. asiaticus'), the suspected causative agent of citrus greening disease, is one of many phloem-restricted plant pathogens that have not been isolated and grown in an axenic culture. In this study, infected Asian citrus psyllids were used to prepare a host-free source of 'Ca. ...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2020.109691
更新日期:2020-12-01 00:00:00
abstract::Progesterone 5β-reductases (P5βRs) are involved in 5β-cardenolide formation by stereo-specific reduction of the △4,5 double bond of steroid precursors. In this study a steroid 5β-reductase was identified in Capsella rubella (CrSt5βR1) and its function in steroid 5β-reduction was validated experimentally. CrSt5βR1 is c...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2019.109483
更新日期:2020-03-01 00:00:00
abstract::In this study, distillery wastewater was treated by dark fermentation or photofermentation alone, and by sequential dark and photofermentation processes using anaerobic saccharolytic consortium and purple nonsulfur bacteria. Combination of dark and photofermentation resulted in the maximal H2 yield of 17.6L/L of disti...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2017.11.009
更新日期:2018-03-01 00:00:00
abstract::In this work, porous biochar derived from sugarcane bagasse was prepared and then coated with different amounts of chitosan (C@CS) for cellulase immobilization. Cellulase was covalently immobilized on the support by using glutaraldehyde as a linker. The chemical characteristics and morphology of the samples were deter...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2020.109561
更新日期:2020-08-01 00:00:00
abstract::Alginate was evaluated as an immobilization matrix for enzyme-catalyzed reactions in organic solvents. In contrast to most hydrogels, calcium alginate was found to be stable in a range of organic solvents and to retain the enzyme inside the gel matrix. In hydrophobic solvents, the alginate gel (greater than 95% water)...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/0141-0229(92)90024-i
更新日期:1992-01-01 00:00:00
abstract::Before applying nanotechnologies in biomedical and environmental areas it is advised to study interactions of nanoparticles and other nanomaterials with biomacromolecule present in living system. Moreover there is scarcity of reports on interactions between nanoparticles and biomaterials. In present report a rapid, ec...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2016.06.005
更新日期:2016-10-01 00:00:00
abstract::A novel endoglucanase active on 1,3-1,4-beta-D-glucans was purified to apparent homogeneity from submerged cultures of the moderately thermophilic aerobic fungus Talaromyces emersonii CBS 814.70. The enzyme is a single subunit glycoprotein with M(r) and pI values of 40.7 +/- 0.3 kDa and 4.4, respectively, and an estim...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/s0141-0229(01)00354-4
更新日期:2001-07-05 00:00:00
abstract::Numerous studies have focused on how to obtain high yield of sophorolipids using low-cost materials as substrates, and there has been various work on the experimental methods for purifying lactonic sophorolipids. These studies have not yet obtained satisfied results in combining a low-cost fermentation process and the...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2012.08.002
更新日期:2012-12-10 00:00:00
abstract::The present study investigated the influence of low-shear modeled microgravity (LSMMG) conditions on Listeria monocytogenes stress response (heat, cold, and acid), membrane fatty acid composition, and virulence potential as well as stress-/virulence-associated gene expression. The results showed that LSMMG-cultivated ...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2019.109440
更新日期:2020-02-01 00:00:00
abstract::Phenylalanine ammonia-lyase (PAL) gene plays an important role in the synthesis of flavones, lignin, and other bioactive compounds in living organisms. Inonotus baumii, the only known flavone-producing filamentous fungus, is of great importance in the investigation of flavone metabolic pathways. To study the function ...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2017.10.010
更新日期:2018-05-01 00:00:00
abstract::The stabilization of fungal cellulases by spray drying, the thermal stability of Penicillium occitanis cellulases and the effect of some additives were studied. We observed that CMCase activity presents a good stability at 50 degrees C, even after 60 h of incubation. On the other hand, beta-glucosidase activity was mo...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/s0141-0229(00)00322-7
更新日期:2001-02-01 00:00:00
abstract::Production of laccase activity by nine strains of Chalara paradoxa isolated from olive mill wastewater disposal ponds were studied. Enzyme extracts obtained from cultured broths by adsorption on hydroxyapatite showed a single band of laccase activity on ABTS after polyacrylamide gel electrophoresis (PAGE). They showed...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/s0141-0229(99)00197-0
更新日期:2000-04-01 00:00:00
abstract::This review focuses on the description of recently discovered esterase enzymes involved in xylan degradation (acetyl xylan, feruloyl, and p-coumaroyl esterases). The occurrence of these enzymes in various microorganisms, assays used for determination of their activity, induction and production on different substrates,...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章,评审
doi:10.1016/0141-0229(93)90078-g
更新日期:1993-06-01 00:00:00
abstract::The kinetic and thermodynamic parameters of wheat β-amylase (WBA) were characterized and various additives were evaluated for enhancing its activity and thermostability. WBA activity was examined by neocuproine method using soluble starch as substrate. The Michaelis constant (K(m)) and molecular activity (k(cat)) were...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/j.enzmictec.2012.07.004
更新日期:2012-10-10 00:00:00
abstract::The kinetics of erythromycin production were dependent on the identity of the growth rate-limiting nutrient during batch cultures of Saccharopolyspora erythraea. Semilogarithmic linear regression provided a single estimate of growth rate during the exponential phase, but partial cubic spline curve fit derivatives prov...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/0141-0229(93)90065-a
更新日期:1993-08-01 00:00:00
abstract::The characterization of the S' subsite specificity of native and ethylated alpha-chymotrypsin has been studied via acyl transfer reaction in acetonitrile containing 10 vol% of water. Using Ac-Tyr-OEt as acyl donor, we investigated the partitioning of acyl-chymotrypsins between water and amino acid and peptide-derived ...
journal_title:Enzyme and microbial technology
pub_type: 杂志文章
doi:10.1016/0141-0229(94)90125-2
更新日期:1994-07-01 00:00:00