Abstract：
:An intracellular alcohol oxidase (AOX) was isolated from the white-rot basidiomycete Phanerochaete chrysosporium (Pch), grown on l-lactate induction medium, and purified to electrophoretic homogeneity. The dimeric protein consisted of two identical 75kDa subunits. The open reading frame of 1,956bp resulted in a monomer consisting of 651 amino acids. The enzyme showed a pI at 5.4, a pH optimum of 9, a temperature optimum at 50°C, possessed putative conserved domains of the GMC superfamily, a FAD binding domain, and showed up to 86% homology to alcohol oxidase sequences of Gloeophyllum trabeum and Coprinopsis cinerea. As was shown for the first time for an AOX from a basidiomycete, not only methanol, but also lower primary alcohols and glycerol were accepted as substrates. An assay based on aldehyde dehydrogenase confirmed d-glyceraldehyde as the product of the reaction. A bioprocess based on this enzyme could alleviate the problems associated with the huge side-stream of glycerol occurring during the manufacture of biodiesel, yielding the green oxidant hydrogen peroxide.
journal_name
Enzyme Microb Technoljournal_title
Enzyme and microbial technologyauthors
Linke D,Lehnert N,Nimtz M,Berger RGdoi
10.1016/j.enzmictec.2014.04.001subject
Has Abstractpub_date
2014-07-01 00:00:00pages
7-12eissn
0141-0229issn
1879-0909pii
S0141-0229(14)00074-Xjournal_volume
61-62pub_type
杂志文章abstract：:In our previous study, we produced α-keto acids by using an L-amino acid deaminase PmiLAAD (wide-type) from Proteus mirabilis, however, the catalytic efficiency was low due to its low substrate affinity. In this study, protein engineering of PmiLAAD was performed to improve the α-keto acid production. PmiLAAD was engi...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/j.enzmictec.2019.109393
更新日期：2020-01-01 00:00:00
abstract：:Zymomonas mobilis genes encoding INVA and INVB were expressed in Pichia pastoris, under the control of the strong AOX1 promoter, and the recombinant enzymes were named INVAAOX1 and INVBAOX1. The expression levels of INVAAOX1 (1660 U/mg) and INVBAOX1 (1993 U/mg) in P. pastoris were 9- and 7-fold higher than those obser...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/j.enzmictec.2015.11.008
更新日期：2016-02-01 00:00:00
abstract：:Direct cellobiose production from cellulose by a genetically modified fungus-Neurospora crassa, was explored in this study. A library of N. crassa sextuple beta-glucosidase (bgl) gene deletion strains was constructed. Various concentrations of cellobiose were detected in the culture broth of the N. crassa sextuple bet...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/j.enzmictec.2012.12.010
更新日期：2013-03-05 00:00:00
abstract：:An extracellular esterase from the actinomycetes Amycolatopsis orientalis was found by screening. It is capable of splitting the isomeric mixture (K/J) of (I, Scheme 1) into 7-amino-3-methoxymethyl-3-cephem-4-carboxylic acid, pivalic acid, and acetaldehyde with a high yield. The purified enzyme of 55.4 Kd by SDS-PAGE ...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/0141-0229(95)00171-9
更新日期：1996-07-01 00:00:00
abstract：:Non-productive cellulase adsorption onto lignin is a major inhibitory mechanism preventing enzymatic hydrolysis of lignocellulosic feedstocks. Therefore, understanding of enzyme-lignin interactions is essential for the development of enzyme mixtures and processes for lignocellulose hydrolysis. We have studied cellulas...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/j.enzmictec.2013.07.003
更新日期：2013-10-10 00:00:00
abstract：:Industrial use of Novozym 435 in synthesis of structured lipids and biodiesel via alcoholysis is limited by mass transfer effects of the glycerides through immobilized enzymes and its low operational stability under operation conditions. To better understand this, differently modified Novozym 435 preparations, differi...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/j.enzmictec.2016.04.007
更新日期：2016-08-01 00:00:00
abstract：:We previously constructed three recombinant phyA mutant strains (PP-NPm-8, PP-NPep-6A and I44E/T252R-PhyA), showing improved catalytic efficiency or thermostability of Aspergillus niger N25 phytase, by error-prone PCR or site-directed mutagenesis. In this study, directed evolution and site-directed mutagenesis were fu...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/j.enzmictec.2017.09.010
更新日期：2018-01-01 00:00:00
abstract：:Genetic engineering has been used to modify the proportion of different cellulases produced by a hypercellulolytic Trichoderma reesei mutant strain. A general expression vector, pAMH110, containing the promoter and terminator sequences of the strongly expressed main cellobiohydrolase 1 (cbh1) gene was used to overexpr...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/0141-0229(91)90133-u
更新日期：1991-03-01 00:00:00
abstract：:Cystathionine γ-lyase (CGL) is a key enzyme in the methionine-cysteine cycle in all living organisms forming cysteine, α-ketobutyrate and ammonia via homocysteine and cystathionine intermediates. Although, human and plant CGLs have been extensively studied at the molecular and mechanistic levels, there has been little...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/j.enzmictec.2015.08.004
更新日期：2015-12-01 00:00:00
abstract：:Foaming, a proficient method for the recovery of surface active solutes from dilute solutions, was successfully applied for the concentration of the lipopeptide biosurfactant Surfactin from B. subtilis ATCC 21332 cell culture broths. Foaming was only partially successful in concentrating Surfactin when applied as a se...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/s0141-0229(00)00327-6
更新日期：2001-03-08 00:00:00
abstract：:2,5-Bis(hydroxymethyl)furan (BHMF) is a versatile building block in the synthesis of polymers, fuels, and macrocycle polyethers. In this work, alcohol dehydrogenases (ADHs) were identified from Meyerozyma guilliermondii SC1103 and were heterologously expressed in Saccharomyces cerevisiae for the synthesis of BHMF from...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/j.enzmictec.2019.109491
更新日期：2020-03-01 00:00:00
abstract：:A novel magnetic Fe3O4 nanoparticles (MNPs) coupled with agarose (AMNPs) was synthesized using co-precipitation via alkaline condition and span-80 surfactants in organic solvent. Iminodiacetate was first attached to the MNPs through epichlorohydrin agent and then chelated with metal ions. The morphology and chemical p...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/j.enzmictec.2014.05.008
更新日期：2014-09-01 00:00:00
abstract：:Different lipases have been immobilized on octyl agarose beads at 1mg/g and at maximum loading, via physical interfacial activation versus the octyl layer on the support. The stability of the preparations was analyzed. Most biocatalysts had the expected result: the apparent stability increased using the highly loaded ...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/j.enzmictec.2016.12.002
更新日期：2017-03-01 00:00:00
abstract：:The filamentous fungal strains Aspergillus niger I-1472 and Pycnoporus cinnabarinus MUCL39533, previously selected for the bioconversion of ferulic acid to vanillic acid and vanillin respectively, were grown on sugar beet pulp. A large spectrum of polysaccharide-degrading enzymes was produced by A. niger and very few ...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/s0141-0229(00)00277-5
更新日期：2001-01-02 00:00:00
abstract：:A method to quantify the impact of proteolysis on accumulation of recombinant proteins in E. coli is described. A much smaller intracellular concentration of staphylococcal protein A (SpA) (14.7 mg. g(-1)) compared to the fusion protein SpA-betagalactosidase (138 mg. g(-1)) is explained by a very high proteolysis rate...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/s0141-0229(00)00294-5
更新日期：2000-12-01 00:00:00
abstract：:Gamma glutamyl transpeptidase from Bacillus pumilus KS12 (GGTBP) was cloned, expressed in pET-28-E. coli expression system as a heterodimeric enzyme with molecular weights of 45 and 20 kDa for large and small subunit, respectively. It was purified by nickel affinity chromatography with hydrolytic and transpeptidase ac...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/j.enzmictec.2011.08.005
更新日期：2012-03-10 00:00:00
abstract：:The activities (at pH 7 and 50 degrees C) of purified EGV (Humicola insolens) and CenA (Cellulomonas fimi) were determined on cotton fabrics at high and low levels of mechanical agitation. Similar activity measurements were also made by using the core domains of these cellulases. Activity experiments suggested that th...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/s0141-0229(00)00205-2
更新日期：2000-08-01 00:00:00
abstract：:Electricity production from carbon monoxide (CO) is demonstrated in a single chamber microbial fuel cell (MFC) with a CoTMPP-based air cathode. The MFC was inoculated with anaerobic sludge and continuously sparged with CO as a sole carbon source. Volumetric power output was maximized at a CO flow rate of 4.8LLR(-1)d(-...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/j.enzmictec.2010.02.010
更新日期：2010-05-05 00:00:00
abstract：:A novel endoglucanase active on 1,3-1,4-beta-D-glucans was purified to apparent homogeneity from submerged cultures of the moderately thermophilic aerobic fungus Talaromyces emersonii CBS 814.70. The enzyme is a single subunit glycoprotein with M(r) and pI values of 40.7 +/- 0.3 kDa and 4.4, respectively, and an estim...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/s0141-0229(01)00354-4
更新日期：2001-07-05 00:00:00
abstract：:A method to stably immobilize microbes on electrodes was developed. Resting cells of Methylobacterium extorquens AM1(MeAM1) were caged within multiwalled carbon nanotubes (MWNTs)by adding the cells to a water dispersion of MWNTs then allowing the resulting mixture to dry on electrodes. The MeAM1-MWCNTs electrode thus ...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/j.enzmictec.2018.06.004
更新日期：2018-10-01 00:00:00
abstract：:Addition of non-ionic surfactants (NIS) is known to accelerate enzymatic lignocellulose hydrolysis. The mechanism behind this accelerating effect is still not elucidated but has been hypothesized to originate from favorable NIS-lignin interactions which alleviate non-productive adsorption of cellulases to lignin. In t...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/j.enzmictec.2011.06.015
更新日期：2011-09-10 00:00:00
abstract：:Nitrification kinetics of synthetic saline wastewater was investigated by using an activated sludge unit. Initial experiments were performed with salt-free wastewater to obtain baseline information. Experiments with 3% salt concentration were performed at different sludge ages in order to investigate the system perfor...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/s0141-0229(01)00312-x
更新日期：2001-05-07 00:00:00
abstract：:The effect of hexoses (glucose and galactose) addition to the feed xylose mineral medium of Debaryomyces hansenii chemostat cultures grown at a constant dilution rate of 0.055 h(-1) was studied. Xylitol was the major product detected amongst all tested conditions. The maximal values for xylitol yield and volumetric pr...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/s0141-0229(00)00166-6
更新日期：2000-06-01 00:00:00
abstract：:Di-, tri-, and tetra-tyrosine peptides with angiotensin I-converting enzyme inhibitory activity were synthesized by papain-catalyzed polymerization of L-tyrosine ethyl ester in aqueous media at 30 °C. Varying the reaction pH from 6.0 to 7.5 and the initial concentration of the ester substrate from 25 to 100 mM, the hi...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/j.enzmictec.2015.03.007
更新日期：2015-07-01 00:00:00
abstract：:This study aimed to develop viable enzymes for bioconversion of resveratrol-glucoside into resveratrol. Out of 13 bacterial strains tested, Lactobacillus kimchi JB301 could completely convert polydatin into resveratrol. The purified enzyme had an optimum temperature of 30-40°C and optimum pH of pH 5.0 against polydati...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/j.enzmictec.2014.09.001
更新日期：2014-12-01 00:00:00
abstract：:Different whole-cell immobilization methods and conditions were tested for the delta 1-dehydrogenation of 6-alpha-methyl-hydrocortisone-21-acetate with Arthrobacter simplex cells, in n-decane-1-ol and chloroform. Among the entrapment methods, polyurethane foams gave the best productivity of n-decane-1-ol, but could no...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/0141-0229(92)90036-n
更新日期：1992-08-01 00:00:00
abstract：:Sialic acid aldolases (E.C.4.1.3.3) catalyze the reversible aldol cleavage of N-acetyl-d-neuraminic acid (Neu5Ac) to from N-acetyl-d-mannosamine (ManNAc) and pyruvate. In this study, a sialic acid aldolase (PdNAL) from Peptoclostridium difficile NAP08 was expressed in Escherichia coli BL21 (DE3). This homotetrameric e...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/j.enzmictec.2016.07.003
更新日期：2016-10-01 00:00:00
abstract：:Nine proteins with lignin peroxidase activity were separated from cultures of Phanerochaete chrysosporium INA-12 in glycerol as carbon source and non-nitrogen limited. Four lignin peroxidase isozymes (4, 5, 8, 9) were purified and characterized. Although differences in kinetic parameters could be shown, antibody react...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/0141-0229(90)90056-v
更新日期：1990-06-01 00:00:00
abstract：:Epoxide hydrolases (EHs) catalyse the conversion of epoxides into vicinal diols. These enzymes have extensive value in biocatalysis as they can generate enantiopure epoxides and diols which are important and versatile synthetic intermediates for the fine chemical and pharmaceutical industries. Despite these benefits, ...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/j.enzmictec.2020.109592
更新日期：2020-09-01 00:00:00
abstract：:Marine microbes have gained significant attention as potential biofactories for broad spectrum bioactive compounds. In the recent years, bioactive biosurfactants have warranted renewed interest from both environmental and clinical sectors as anti-biofilm agents due to their excellent properties of dispersing microbial...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/j.enzmictec.2018.09.008
更新日期：2019-01-01 00:00:00
