当前位置： SCI文献检索 > ENZYME AND MICROBIAL TECHNOLOGY期刊下所有文献 > Enhancing catalytic performance of β-glucosidase via immobilization on metal ions chelated magnetic nanoparticles.

Enhancing catalytic performance of β-glucosidase via immobilization on metal ions chelated magnetic nanoparticles.

Abstract：

:A novel magnetic Fe3O4 nanoparticles (MNPs) coupled with agarose (AMNPs) was synthesized using co-precipitation via alkaline condition and span-80 surfactants in organic solvent. Iminodiacetate was first attached to the MNPs through epichlorohydrin agent and then chelated with metal ions. The morphology and chemical properties of these prepared supports were characterized by scanning electron microscopy (SEM), X-ray power diffraction (XRD), vibrating sample magnetometer (VSM), and Fourier transform infrared spectroscopy (FT-IR). Among them, the Co(2+)-chelated AMNPs (AMNPs-ECH-IDA-Co(2+)) showed the second highest enzyme adsorption capacity of 1.81 mg/g particles, and achieved the largest activity recovery of 117% per protein gram in immobilization of β-glucosidase (BGL). The Michaelis constant (Km) and Vmax of the immobilized BGL were 0.904 mM and 0.057 μmol/min, respectively, and its activation energy was much lower than the free form. Moreover, the immobilized enzyme exhibited enhanced thermostability and operational stability. It still retained more than 90% of its initial activity after being operated for 15 successive batches. This study demonstrates that the immobilized β-glucosidase has a good prospect in industrial applications.

journal_name

Enzyme Microb Technol

journal_title

Enzyme and microbial technology

authors

Chen T,Yang W,Guo Y,Yuan R,Xu L,Yan Y

doi

10.1016/j.enzmictec.2014.05.008

subject

Has Abstract

pub_date

2014-09-01 00:00:00

pages

50-7

eissn

0141-0229

issn

1879-0909

pii

S0141-0229(14)00116-1

journal_volume

63

pub_type

杂志文章

相关文献

ENZYME AND MICROBIAL TECHNOLOGY文献大全
  • Intermittent light irradiation with second- or hour-scale periods controls anthocyanin production by strawberry cells*

    abstract：:Anthocyanin production by strawberry cells depends not only on light intensity but also on the light/dark cycle operation with hour- or second-scale periods. These findings are useful for designing and operating photobioreactors for enhanced anthocyanin production. Intermittent illumination with a second-scale period ...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/s0141-0229(00)00143-5

    authors： Kurata H,Mochizuki A,Okuda N,Seki M,Furusaki S

    更新日期：2000-05-01 00:00:00

  • Comments on "Bioprocessing in space"

    abstract：:An analysis developed by Westgate et al. for the digestible energy of edible and inedible biomass, including hydrolysis and fermentation, is reexamined with state-of-the-art values for the harvest index of hydroponic crops. ...

    journal_title：Enzyme and microbial technology

    pub_type： 评论,杂志文章

    doi：10.1016/0141-0229(93)90104-a

    authors： Volk T

    更新日期：1993-10-01 00:00:00

  • Improved efficiency of a novel methyl parathion hydrolase using consensus approach.

    abstract：:A methyl parathion hydrolase (MPH) gene, bjmpd, was cloned from a newly isolated MP-degrading bacterial strain, Burkholderia jiangsuensis MP-1T and heterologously expressed in Escherichia coli BL21 (DE3). Although the amino acid sequence of the bjmpd-encoded enzyme, named BjMPH, differed from that of MPH from Pseudomo...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2016.07.005

    authors： Liu XY,Chen FF,Li CX,Luo XJ,Chen Q,Bai YP,Xu JH

    更新日期：2016-11-01 00:00:00

  • Optimization and modelling of enzymatic polymerization of ε-caprolactone to polycaprolactone using Candida Antartica Lipase B with response surface methodology and artificial neural network.

    abstract：:Recently enzymatic catalysts have replaced organic and organometallic catalysts in the synthesis of bio-resorbable polymers. Enzymatic polymerization is considered as an alternative to conventional polymerization as they are less toxic, environmental friendly and can operate under mild conditions. In this research, th...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2018.12.001

    authors： Pakalapati H,Tariq MA,Arumugasamy SK

    更新日期：2019-03-01 00:00:00

  • Increasing the refolding efficiency in vitro by site-directed mutagenesis of Cys383 in rat procarboxypeptidase B.

    abstract：:This study examines a novel method to reduce the probability of disulfide mismatches during the refolding process by the replacement of cysteines within a protein. Specifically, Cys383 of recombinant rat procarboxypeptidase B was replaced by other amino acids to increase the refolding efficiency in vitro. Mutants C383...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2011.04.018

    authors： Li S,Zhang L,Wu Q,Xin A,Zhao J,Fan L

    更新日期：2011-07-10 00:00:00

  • Whole-cell bio-oxidation of n-dodecane using the alkane hydroxylase system of P. putida GPo1 expressed in E. coli.

    abstract：:The alkane-1-monoxygenase (alkB) complex of Pseudomonas putida GPo1 has been extensively studied in the past and shown to be capable of oxidising aliphatic C(5)-C(12) alkanes to primary alcohols both in the wild-type organism by growth on C(5)-C(12) alkanes as sole carbon source and in vitro. Despite this, successful ...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2011.01.008

    authors： Grant C,Woodley JM,Baganz F

    更新日期：2011-05-06 00:00:00

  • Absence or presence of metal ion activation in two structurally similar GH43 β-xylosidases.

    abstract：:Two GH43 β-xylosidases, RS223-BX from a rice straw metagenomic library, and BoXA from Bacteroides ovatus, that share similar amino acid sequences (81% identical) and 19 of 20 active-site residues, were compared by using site-directed mutagenesis of Asp and His residues implicated in metal binding. Thus, RS223-BX is st...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2018.03.007

    authors： Jordan DB,Stoller JR,Kibblewhite RE,Chan VJ,Lee CC,Wagschal K

    更新日期：2018-07-01 00:00:00

  • Transient behavior of T-cell hybridomas in response to changes in metabolite concentrations in continuous culture.

    abstract：:Effects of pulse and step changes in major metabolite concentrations such as glucose, glutamine, lactic acid, and ammonium on behavior of T-cell hybridomas in continuous culture were investigated in order to develop a strategy to maximize specific rate of lymphokine formation. Step increases in glucose, lactic acid, a...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/0141-0229(90)90007-d

    authors： Shaughnessy TS,Kargi F

    更新日期：1990-09-01 00:00:00

  • Magnetosomes extracted from Magnetospirillum magneticum strain AMB-1 showed enhanced peroxidase-like activity under visible-light irradiation.

    abstract：:Magnetosomes are intracellular structures produced by magnetotactic bacteria and are magnetic nanoparticles surrounded by a lipid bilayer membrane. Magnetosomes reportedly possess intrinsic enzyme mimetic activity similar to that found in horseradish peroxidase (HRP) and can scavenge reactive oxygen species depending ...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2015.02.009

    authors： Li K,Chen C,Chen C,Wang Y,Wei Z,Pan W,Song T

    更新日期：2015-05-01 00:00:00

  • Improving the thermostability of a GH11 xylanase by directed evolution and rational design guided by B-factor analysis.

    abstract：:Operational stability under high temperature is required for enzyme application in industrial processes. Error-prone PCR and B-factor analysis were employed to enhance the thermostability of a xylanase from GH family 11 in this study. Based on the top 10 mutants screened from the random mutation libraries, mutant Xyn3...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2020.109720

    authors： Xing H,Zou G,Liu C,Chai S,Yan X,Li X,Liu R,Yang Y,Zhou Z

    更新日期：2021-02-01 00:00:00

  • Increase in the thermostability of GH11 xylanase XynJ from Bacillus sp. strain 41M-1 using site saturation mutagenesis.

    abstract：:GH11 xylanase XynJ from Bacillus sp. strain 41M-1 has a β-jellyroll fold composed of eight β strands with a deep active-site cleft. We hypothesized that the thermostability of XynJ will increase if the flexibility of the β strands in the jellyroll structure is decreased without impairing activity. To verify this hypot...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2019.109363

    authors： Takita T,Nakatani K,Katano Y,Suzuki M,Kojima K,Saka N,Mikami B,Yatsunami R,Nakamura S,Yasukawa K

    更新日期：2019-11-01 00:00:00

  • Agitation rate effects on plasmid stability in immobilized and free-cell continuous cultures of recombinant E. coli.

    abstract：:Escherichia coli B/pTG201 recombinant cells were immobilized by entrapment in a carrageenan gel and cultivated in nonselective media to investigate the effect of agitation rate on plasmid stability, biomass concentration, and enzyme productivity. These parameters were studied in continuous cultures for free and immobi...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/0141-0229(90)90112-4

    authors： Huang J,Dhulster P,Thomas D,Barbotin JN

    更新日期：1990-12-01 00:00:00

  • An efficient in vitro refolding of recombinant bacterial laccase in Escherichia coli.

    abstract：:Laccases (benzenediol oxygen oxidoreductases, EC 1.10.3.2) are important multicopper enzymes that are used in many biotechnological processes. A recombinant form of laccase from Bacillus sp. HR03 was overexpressed in Escherichia coli BL-21(DE3). Inclusion body (IB) formation happens quite often during recombinant prot...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2013.03.006

    authors： Mollania N,Khajeh K,Ranjbar B,Rashno F,Akbari N,Fathi-Roudsari M

    更新日期：2013-05-10 00:00:00

  • Enhancement of neuroprotection, antioxidant capacity, and water-solubility of crocins by transglucosylation using dextransucrase under high hydrostatic pressure.

    abstract：:Crocin, one of the major carotenoid pigments of Crocus sativus (saffron), is responsible for antioxidant activity, neuroprotection, and the inhibition of tumor cell proliferation. In order to improve the functionality of crocin, α-glucosyl-(1→6)-trans-crocins (C-Gs) were synthesized using sucrose and dextransucrase fr...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2020.109630

    authors： Mok IK,Nguyen TTH,Kim DH,Lee JW,Lim S,Jung HY,Lim T,Pal K,Kim D

    更新日期：2020-10-01 00:00:00

  • Aspergillus niger I-1472 and Pycnoporus cinnabarinus MUCL39533, selected for the biotransformation of ferulic acid to vanillin, are also able to produce cell wall polysaccharide-degrading enzymes and feruloyl esterases.

    abstract：:The filamentous fungal strains Aspergillus niger I-1472 and Pycnoporus cinnabarinus MUCL39533, previously selected for the bioconversion of ferulic acid to vanillic acid and vanillin respectively, were grown on sugar beet pulp. A large spectrum of polysaccharide-degrading enzymes was produced by A. niger and very few ...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/s0141-0229(00)00277-5

    authors： Bonnina E,Brunel M,Gouy Y,Lesage-Meessen L,Asther M,Thibault J

    更新日期：2001-01-02 00:00:00

  • Construction of glutamate biosensor based on covalent immobilization of glutamate oxidase on polypyrrole nanoparticles/polyaniline modified gold electrode.

    abstract：:A method is described for construction of a highly sensitive electrochemical biosensor for detection of glutamate. The biosensor is based on covalent immobilization of glutamate oxidase (GluOx) onto polypyrrole nanoparticles and polyaniline composite film (PPyNPs/PANI) electrodeposited onto Au electrode. The enzyme el...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2014.02.001

    authors： Batra B,Kumari S,Pundir CS

    更新日期：2014-04-10 00:00:00

  • Effect of protein load on stability of immobilized enzymes.

    abstract：:Different lipases have been immobilized on octyl agarose beads at 1mg/g and at maximum loading, via physical interfacial activation versus the octyl layer on the support. The stability of the preparations was analyzed. Most biocatalysts had the expected result: the apparent stability increased using the highly loaded ...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2016.12.002

    authors： Fernandez-Lopez L,Pedrero SG,Lopez-Carrobles N,Gorines BC,Virgen-Ortíz JJ,Fernandez-Lafuente R

    更新日期：2017-03-01 00:00:00

  • On-line chemiluminescence assay using FIA and fiber optics for urinary and blood glucose.

    abstract：:A chemiluminescence fiber optic system coupled to flow injection analysis (FIA) and ion exchange chromatography has been developed for determining glucose in blood and urine. Immobilized glucose oxidase acted on beta-D-glucose to produce hydrogen peroxide, which was then reacted with luminol in the presence of ferricy...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/0141-0229(93)90130-t

    authors： Cattaneo MV,Luong JH

    更新日期：1993-05-01 00:00:00

  • Chaperon solvent plug design in size-exclusion chromatography protein refolding process.

    abstract：:Although the chaperon solvent plug was reported as a strategy to reduce aggregation before the column inlet in SEC (size-exclusion chromatography) protein refolding process, the appropriate position at which sample injected and the volume of the chaperon solvent plug have not been elucidated. Therefore, the detail of ...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2011.05.003

    authors： Chen YC,Liu HS

    更新日期：2011-07-10 00:00:00

  • Discovery and characterization of new O-methyltransferase from the genome of the lignin-degrading fungus Phanerochaete chrysosporium for enhanced lignin degradation.

    abstract：:Using bioinformatic homology search tools, this study utilized sequence phylogeny, gene organization and conserved motifs to identify members of the family of O-methyltransferases from lignin-degrading fungus Phanerochaete chrysosporium. The heterologous expression and characterization of O-methyltransferases from P. ...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2015.08.016

    authors： Thanh Mai Pham L,Kim YH

    更新日期：2016-01-01 00:00:00

  • Enhanced bioconversion rate and released substrate inhibition in (R)-phenylephrine whole-cell bioconversion via partial acetone treatment.

    abstract：:An approach was developed to enhance the efficiency for the bioconversion of 1-(3-hydroxyphenyl)-2-(methyamino)-ethanone to (R)-phenylephrine. The strain Serratia marcescens N10612, giving the benefit of 99% enantiomeric excess in (R)-PE conversion, was used. The fermentation was devised to harvest cells with high hyd...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2015.11.004

    authors： Kan SC,Zang CZ,Yeh CW,Chang WF,Lin CC,Hung TH,Shieh CJ,Liu YC

    更新日期：2016-05-01 00:00:00

  • Improvement of catalytical properties of two invertases highly tolerant to sucrose after expression in Pichia pastoris. Effect of glycosylation on enzyme properties.

    abstract：:Zymomonas mobilis genes encoding INVA and INVB were expressed in Pichia pastoris, under the control of the strong AOX1 promoter, and the recombinant enzymes were named INVAAOX1 and INVBAOX1. The expression levels of INVAAOX1 (1660 U/mg) and INVBAOX1 (1993 U/mg) in P. pastoris were 9- and 7-fold higher than those obser...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2015.11.008

    authors： Pérez de los Santos AI,Cayetano-Cruz M,Gutiérrez-Antón M,Santiago-Hernández A,Plascencia-Espinosa M,Farrés A,Hidalgo-Lara ME

    更新日期：2016-02-01 00:00:00

  • Progress in using threonine aldolases for preparative synthesis.

    abstract：:Three threonine aldolases (TAs) were cloned and overexpressed in Escherichia coli (Aeromonas jandaeil-allo-threonine aldolase, E. colil-threonine aldolase and Thermotoga maritimal-allo-threonine aldolase). A Design of Experiments strategy was used to identify optimal reaction conditions for each enzyme. These conditio...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2018.07.004

    authors： Beaudoin SF,Hanna MP,Ghiviriga I,Stewart JD

    更新日期：2018-12-01 00:00:00

  • The development and application of a new affinity partitioning system for enzyme isolation and purification.

    abstract：:A reactive water-soluble polymer was synthesized by copolymerizing N-isopropylacrylamide and glycidyl acrylate. The reactive polymer could react with the amino groups of enzymes/proteins or other ligands to form an affinity polymer. As a model, the reactive polymer was allowed to react with paraaminobenzamidine, a str...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/0141-0229(90)90005-b

    authors： Nguyen AL,Luong JH

    更新日期：1990-09-01 00:00:00

  • Optimization of the growth conditions of Sf21 insect cells for high-density perfusion culture in stirred-tank bioreactors.

    abstract：:Spodoptera frugiperda insect cells (IPLB-Sf21-AE) (Sf21), infected with baculovirus expression vectors during their exponential growth phase, are commonly used to produce a variety of heterologous recombinant proteins. In the present study the culture conditions of these insect cells were studied to establish high-den...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/0141-0229(94)90022-1

    authors： Deutschmann SM,Jäger V

    更新日期：1994-06-01 00:00:00

  • Screening of microbes for novel acidic cutinases and cloning and expression of an acidic cutinase from Aspergillus niger CBS 513.88.

    abstract：:Isolates from gardening waste compost and 38 culture collection microbes were grown on agar plates at pH 4.0 with the cutinase model substrate polycaprolactone as a carbon source. The strains showing polycaprolactone hydrolysis were cultivated in liquid at acidic pH and the cultivations were monitored by assaying the ...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2013.01.005

    authors： Nyyssölä A,Pihlajaniemi V,Järvinen R,Mikander S,Kontkanen H,Kruus K,Kallio H,Buchert J

    更新日期：2013-04-10 00:00:00

  • Membrane filtration biocathode microbial fuel cell for nitrogen removal and electricity generation.

    abstract：:Conductive materials with attached biofilms, were used as membrane filtration biocathodes to filter the effluent and supply electrons for denitrification. Stainless steel mesh and carbon felt were employed to fabricate membrane modules, and the two MFC systems were termed as M1 and M2, respectively. High effluent qual...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2014.04.005

    authors： Zhang G,Zhang H,Ma Y,Yuan G,Yang F,Zhang R

    更新日期：2014-06-10 00:00:00

  • A thermophilic enzymatic cocktail for galactomannans degradation.

    abstract：:The full utilization of hemicellulose sugars (pentose and exose) present in lignocellulosic material, is required for an efficient bio-based fuels and chemicals production. Two recombinant thermophilic enzymes, an endo-1,4-β-mannanase from Dictyoglomus turgidum (DturCelB) and an α-galactosidase from Thermus thermophil...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2017.12.008

    authors： Aulitto M,Fusco FA,Fiorentino G,Bartolucci S,Contursi P,Limauro D

    更新日期：2018-04-01 00:00:00

  • A quenched-flow system for measuring heterogeneous enzyme kinetics with sub-second time resolution.

    abstract：:Even though many enzyme processes occur at the interface of an insoluble substrate, these reactions are generally much less studied than homogenous enzyme reactions in the aqueous bulk. Interfacial (or heterogeneous) enzyme reactions involve several reaction steps, and the established experimental approach to elucidat...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2017.06.009

    authors： Olsen JP,Kari J,Borch K,Westh P

    更新日期：2017-10-01 00:00:00

  • Kinetic resolution of sec-alcohols catalysed by Candida antarctica lipase B displaying Pichia pastoris whole-cell biocatalyst.

    abstract：:Kinetic resolution of sec-alcohols is a green process with biocatalyst. Candida antarctica lipase B (CALB) displayed on Pichia pastoris cell-surface (Pp-CALB) was characterized in kinetic resolution of sec-alcohols with different structures. The reaction parameters including acyl donors, molar ratio of substrates, sol...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2017.11.005

    authors： Zhang K,Pan Z,Diao Z,Liang S,Han S,Zheng S,Lin Y

    更新日期：2018-03-01 00:00:00