An efficient in vitro refolding of recombinant bacterial laccase in Escherichia coli.

abstract：:The alkane-1-monoxygenase (alkB) complex of Pseudomonas putida GPo1 has been extensively studied in the past and shown to be capable of oxidising aliphatic C(5)-C(12) alkanes to primary alcohols both in the wild-type organism by growth on C(5)-C(12) alkanes as sole carbon source and in vitro. Despite this, successful ...

journal_title：Enzyme and microbial technology

authors： Grant C,Woodley JM,Baganz F

更新日期：2011-05-06 00:00:00

abstract：:An extracellular esterase from the actinomycetes Amycolatopsis orientalis was found by screening. It is capable of splitting the isomeric mixture (K/J) of (I, Scheme 1) into 7-amino-3-methoxymethyl-3-cephem-4-carboxylic acid, pivalic acid, and acetaldehyde with a high yield. The purified enzyme of 55.4 Kd by SDS-PAGE ...

journal_title：Enzyme and microbial technology

authors： Sauber K,Aretz W,Meiwes J,Wollmann T

更新日期：1996-07-01 00:00:00

abstract：:Industrial use of Novozym 435 in synthesis of structured lipids and biodiesel via alcoholysis is limited by mass transfer effects of the glycerides through immobilized enzymes and its low operational stability under operation conditions. To better understand this, differently modified Novozym 435 preparations, differi...

journal_title：Enzyme and microbial technology

authors： Villalba M,Verdasco-Martín CM,Dos Santos JC,Fernandez-Lafuente R,Otero C

更新日期：2016-08-01 00:00:00

abstract：:Mycobacterium tuberculosis acetohydroxyacid synthase (M. tuberculosis AHAS) has been proposed to bean essential target for novel herbicide- and chemical-based antibacterial agents. Therefore, here we investigated the roles of multiple conserved herbicide-binding site residues (R318, A146, Q148, M512, and V513) in M. t...

journal_title：Enzyme and microbial technology

authors： Jung IP,Cho JH,Koo BS,Yoon MY

更新日期：2015-10-01 00:00:00

abstract：:Kumamolisin from Alicyclobacillus sendaiensis strain NTAP-1 is a serine protease with collagenase activity. After molecular engineering, a kumamolisin mutant, named Kuma030, was obtained with high proteolytic activity against gluten, which might cause celiac disease. Kuma030 exhibited its potential application in indu...

journal_title：Enzyme and microbial technology

authors： Liu H,Fan X,Song H,Hu X,Zhang G,Yu C,Yi L

更新日期：2019-10-01 00:00:00

abstract：:Growth conditions for mass production of luciferase-bacterial magnetic particles (BMPs) by a recombinant Magnetospirillum magneticum AMB-1 were investigated in a pH-regulated fed-batch culture system. Enrichment of growth medium with L-cysteine, yeast extract and polypeptone enhanced both bacterial growth and BMP prod...

journal_title：Enzyme and microbial technology

authors： Yang C,Takeyama H,Tanaka T,Matsunaga T

更新日期：2001-07-05 00:00:00

abstract：:'Candidatus Liberibacter asiaticus' ('Ca. L. asiaticus'), the suspected causative agent of citrus greening disease, is one of many phloem-restricted plant pathogens that have not been isolated and grown in an axenic culture. In this study, infected Asian citrus psyllids were used to prepare a host-free source of 'Ca. ...

journal_title：Enzyme and microbial technology

authors： Molki B,Call DR,Ha PT,Omsland A,Gang DR,Lindemann SR,Killiny N,Beyenal H

更新日期：2020-12-01 00:00:00

abstract：:We have designed an electrochemical DNA biosensor based on stem-loop structured probes for enzymatic detection of Pseudomonas aeruginosa 16S ribosomal RNA (rRNA) in composting degradation. The probe modified with a thiol at its 5' end and a biotin at its 3' end was immobilized on a gold electrode through self-assembly...

journal_title：Enzyme and microbial technology

authors： Liu C,Zeng GM,Tang L,Zhang Y,Li YP,Liu YY,Li Z,Wu MS,Luo J

更新日期：2011-08-10 00:00:00

abstract：:Gamma glutamyl transpeptidase from Bacillus pumilus KS12 (GGTBP) was cloned, expressed in pET-28-E. coli expression system as a heterodimeric enzyme with molecular weights of 45 and 20 kDa for large and small subunit, respectively. It was purified by nickel affinity chromatography with hydrolytic and transpeptidase ac...

journal_title：Enzyme and microbial technology

authors： Murty NA,Tiwary E,Sharma R,Nair N,Gupta R

更新日期：2012-03-10 00:00:00

abstract：:The possibility of using thermostable inulinases from Aspergillus ficuum in place of invertase for sucrose hydrolysis was explored. The commercial inulinases preparation was immobilized onto porous glass beads by covalent coupling using activation by a silane reagent and glutaraldehyde before adding the enzyme. The im...

journal_title：Enzyme and microbial technology

authors： Ettalibi M,Baratti JC

更新日期：2001-05-07 00:00:00

abstract：:Kinetic resolution of sec-alcohols is a green process with biocatalyst. Candida antarctica lipase B (CALB) displayed on Pichia pastoris cell-surface (Pp-CALB) was characterized in kinetic resolution of sec-alcohols with different structures. The reaction parameters including acyl donors, molar ratio of substrates, sol...

journal_title：Enzyme and microbial technology

authors： Zhang K,Pan Z,Diao Z,Liang S,Han S,Zheng S,Lin Y

更新日期：2018-03-01 00:00:00

abstract：:Solid-liquid enzyme reactions constitute important processes in biochemical industries. The isomerization of d-glucose to d-fructose, using the immobilized glucose isomerase (Sweetzyme T), as a typical example of solid-liquid catalyzed reactions has been carried out in one stage and multi-stage novel type of impinging...

journal_title：Enzyme and microbial technology

authors： Fatourehchi N,Sohrabi M,Dabir B,Royaee SJ,Haji Malayeri A

更新日期：2014-02-05 00:00:00

abstract：:A new bi-modular, wide pH spectrum and highly active xylanase KRICT PX3 (JF320814) isolated from Paenibacillus terrae HPL-003 (KCTC11987BP) has been cloned and expressed in Escherichia coli. Purified recombinant xylanase KRICT PX-3 (1,620 bp, 540aa, NCBI accession number JF320814) showed highly active at 55°C in pH 4....

journal_title：Enzyme and microbial technology

authors： Song HY,Lim HK,Kim DR,Lee KI,Hwang IT

更新日期：2014-01-10 00:00:00

abstract：:The primary aim of this study was to decipher the catalytic functions of the NHase with wide substrate spectra from Rhodococcus ruber CGMCC3090 by computer modeling and substrate docking. 3D structure model of the enzyme was built by computer modeling to obtain the optimal structure. The larger binding site cavity (55...

journal_title：Enzyme and microbial technology

authors： Wang S,Dai Y,Wang J,Shen Y,Zhai Y,Zheng H,Wang M

更新日期：2013-02-05 00:00:00

abstract：:3-Mercaptopyruvate (3MPy), a structural analog of 3-mercaptopropionic acid, is a precursor compound for biosynthesis of polythioesters in bacteria. The cost-effectiveness and sustainability of the whole process could be greatly improved by using the cysteine degradation pathway for an intracellular supply of 3MPy. Tra...

journal_title：Enzyme and microbial technology

authors： Andreeßen C,Gerlt V,Steinbüchel A

更新日期：2017-04-01 00:00:00

abstract：:The kinetics of erythromycin production were dependent on the identity of the growth rate-limiting nutrient during batch cultures of Saccharopolyspora erythraea. Semilogarithmic linear regression provided a single estimate of growth rate during the exponential phase, but partial cubic spline curve fit derivatives prov...

journal_title：Enzyme and microbial technology

authors： McDermott JF,Lethbridge G,Bushell ME

更新日期：1993-08-01 00:00:00

abstract：:We demonstrate the feasibility of growing crystals of protein in volumes as small as 1 nanoliter. Advances in the handling of very small volumes (i.e. through inkjet and other technologies) open the way towards fully automated systems. The rationale for these experiments is the desire to develop a system that speeds u...

journal_title：Enzyme and microbial technology

authors： Kuil ME,Bodenstaff ER,Hoedemaeker FJ,Abrahams JP

更新日期：2002-03-13 00:00:00

abstract：:beta-amylases are used in production of maltose syrup. It is shown that sweet potato beta-amylase can be purified by affinity precipitation with alginate with 80% activity yield and 44 fold purification. SDS-PAGE of the purified protein showed a single band and a subunit weight of 50 kDa. Preliminary data with soybean...

journal_title：Enzyme and microbial technology

authors： Teotia S,Khare SK,Gupta MN

更新日期：2001-06-07 00:00:00

abstract：:Two acidic β-glucosidases (βGI and βGII) from the brown rot fungus Fomitopsis palustris were purified to homogeneity by several chromatographic steps. βGI and βGII had molecular weights of 130 and 213 kDa, respectively, and exhibited optimum activity at pH 2.5 and 55°C. The K(m) values of βGI and βGII for p-nitropheny...

journal_title：Enzyme and microbial technology

authors： Okamoto K,Sugita Y,Nishikori N,Nitta Y,Yanase H

更新日期：2011-04-07 00:00:00

abstract：:The lipases from Thermomyces lanuginosus and Pseudomonas cepacia have been immobilized on octyl and cyanogen bromide (CNBr) agarose beads. The immobilization on octyl-agarose is slowed with increasing ionic strength, while the immobilization on CNBr is not significantly affected by the ionic strength. The inhibition o...

journal_title：Enzyme and microbial technology

authors： Manoel EA,Dos Santos JC,Freire DM,Rueda N,Fernandez-Lafuente R

更新日期：2015-04-01 00:00:00

abstract：:This work describes for the first time the green synthesis of neopentyl glycol diheptanoate in a solvent-free medium via an enzymatic pathway. The process has been carried out in an open-air reactor in order to ease water removal through evaporation and shift the chemical equilibrium towards product formation. The inh...

journal_title：Enzyme and microbial technology

authors： Serrano-Arnaldos M,García-Martínez JJ,Ortega-Requena S,Bastida J,Máximo F,Montiel MC

更新日期：2020-01-01 00:00:00

abstract：:The fatty acid methyl esters and fatty acid ethyl esters are known as biodiesels which are considered to be renewable, nontoxic and biodegradable biofuels. However, the conventional biodiesels show a high crystallization temperature which is one of the most critical obstacles against the widespread biodiesel usage. Th...

journal_title：Enzyme and microbial technology

authors： Pan H,Zhang L,Li X,Guo D

更新日期：2017-07-01 00:00:00

abstract：:The parameters of biosensors based on the yeast strains Candida maltosa VKM Y-2359, Candida blankii VKM Y-2675, and Debaryomyces hansenii VKM Y-2482 for biochemical oxygen demand (BOD) detection are compared. The catalytic activity of the strains was analyzed in relation to the growth phase. The possibility of using D...

journal_title：Enzyme and microbial technology

authors： Arlyapov V,Kamanin S,Ponamoreva O,Reshetilov A

更新日期：2012-04-05 00:00:00

abstract：:Long-chain alkyl glucosides, such as octyl and decyl β-d-glucopyranosides (OG and DG, respectively), are regarded as a new generation of biodegradable, non-ionic surfactants. Previously, the mutants of Dalbergia cochinchinensis Pierre dalcochinase showed potential in the synthesis of oligosaccharides and alkyl glucosi...

journal_title：Enzyme and microbial technology

authors： Thenchartanan P,Pitchayatanakorn P,Wattana-Amorn P,Ardá A,Svasti J,Jiménez-Barbero J,Kongsaeree PT

更新日期：2020-10-01 00:00:00

abstract：:Biosensors have been extensively studied during the last 20 years, and a myriad of laboratory biosensors have been developed. Improvements are required in biosensor design and performance before they become widely accepted in industrial process monitoring. However, as the biotechnology industry expands, biosensors may...

journal_title：Enzyme and microbial technology

authors： Brooks SL,Higgins IJ,Newman JD,Turner AP

更新日期：1991-12-01 00:00:00

abstract：:The stabilization of fungal cellulases by spray drying, the thermal stability of Penicillium occitanis cellulases and the effect of some additives were studied. We observed that CMCase activity presents a good stability at 50 degrees C, even after 60 h of incubation. On the other hand, beta-glucosidase activity was mo...

journal_title：Enzyme and microbial technology

authors： Belghith H,Ellouz Chaabouni S,Gargouri A

更新日期：2001-02-01 00:00:00

abstract：:A generic approach for flavoenzyme immobilization was developed in which the flavin cofactor is used for anchoring enzymes onto the carrier. It exploits the tight binding of flavin cofactors to their target apo proteins. The method was tested for phenylacetone monooxygenase (PAMO) which is a well-studied and industria...

journal_title：Enzyme and microbial technology

authors： Krzek M,van Beek HL,Permentier HP,Bischoff R,Fraaije MW

更新日期：2016-01-01 00:00:00

abstract：:By using very active and very stable penicillin G acylase (PGA)--agarose derivatives we have studied the industrial design of equilibrium-controlled synthesis of lactamic antibiotics. In the presence of high concentrations of organic cosolvents we have carried out the direct enzymatic condensation of phenylacetic acid...

journal_title：Enzyme and microbial technology

authors： Fernandez-Lafuente R,Rosell CM,Guisán JM

更新日期：1991-11-01 00:00:00

abstract：:Alginate was evaluated as an immobilization matrix for enzyme-catalyzed reactions in organic solvents. In contrast to most hydrogels, calcium alginate was found to be stable in a range of organic solvents and to retain the enzyme inside the gel matrix. In hydrophobic solvents, the alginate gel (greater than 95% water)...

journal_title：Enzyme and microbial technology

authors： Hertzberg S,Kvittingen L,Anthonsen T,Skjåk-Braek G

更新日期：1992-01-01 00:00:00

abstract：:The selected thermotolerant, lactose-utilizing yeast strain Kluyveromyces marxianus NBIMCC 8362 possesses high specific d-amino acid oxidase activity (60Ug(-1)), which was increased nine-fold (545Ug(-1)) by design of the growth medium and conditions for d-amino oxidase induction. Applying an optimized simple and rapid...

journal_title：Enzyme and microbial technology

authors： Kostova DD,Petrova VY,Kujumdzieva AV

更新日期：2008-01-01 00:00:00