Abstract:
:Solid-liquid enzyme reactions constitute important processes in biochemical industries. The isomerization of d-glucose to d-fructose, using the immobilized glucose isomerase (Sweetzyme T), as a typical example of solid-liquid catalyzed reactions has been carried out in one stage and multi-stage novel type of impinging streams reactors. Response surface methodology was applied to determine the effects of certain pertinent parameters of the process namely axial velocity (A), feed concentration (B), nozzles' flow rates (C) and enzyme loading (D) on the performance of the apparatus. The results obtained from the conversion of glucose in this reactor were much higher than those expected in conventional reactors, while residence time was decreased dramatically. Residence time distribution (RTD) in a one-stage impinging streams reactor was investigated using colored solution as the tracer. The results showed that the flow pattern in the reactor was close to that in a continuous stirred tank reactor (CSTR). Based on the analysis of flow region in the reactor, gamma distribution model with bypass (GDB) was applied to study the RTD of the reactor. The results indicated that RTD in the impinging streams reactor could be described by the latter model.
journal_name
Enzyme Microb Technoljournal_title
Enzyme and microbial technologyauthors
Fatourehchi N,Sohrabi M,Dabir B,Royaee SJ,Haji Malayeri Adoi
10.1016/j.enzmictec.2013.11.006subject
Has Abstractpub_date
2014-02-05 00:00:00pages
14-20eissn
0141-0229issn
1879-0909pii
S0141-0229(13)00246-9journal_volume
55pub_type
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