Growth of 'Candidatus Liberibacter asiaticus' in a host-free microbial culture is associated with microbial community composition.


:'Candidatus Liberibacter asiaticus' ('Ca. L. asiaticus'), the suspected causative agent of citrus greening disease, is one of many phloem-restricted plant pathogens that have not been isolated and grown in an axenic culture. In this study, infected Asian citrus psyllids were used to prepare a host-free source of 'Ca. L. asiaticus'. Host-free mixed microbial cultures of 'Ca. L. asiaticus' were grown in the presence of various antibiotic treatments to alter the composition of the microbial communities. Our hypothesis was that the presence of selected antibiotics would enhance or reduce the presence of 'Ca. L. asiaticus' in a host-free culture composed of a mixed bacterial population through changes in the microbial community structure. We determined how 'Ca. L. asiaticus' growth changed with the various treatments. Treatment with vancomycin (50 μg/mL), streptomycin (0.02 μg/mL), or polymyxin B (4 μg/mL) was associated with an increased abundance of 'Ca. L. asiaticus' of 7.35 ± 0.27, 5.56 ± 0.15, or 4.54 ± 0.83 fold, respectively, compared to untreated mixed microbial cultures, while treatment with 100 μg/mL vancomycin; 0.5, 1, or 2 μg/mL streptomycin; or 0.5 μg/mL of polymyxin B was associated with reduced growth. In addition, the growth of 'Ca. L. asiaticus' was associated with the microbial community composition of the mixed microbial cultures. A positive relationship between the presence of the Pseudomonadaceae family and 'Ca. L. asiaticus' growth was observed, while the presence of 'Ca. L. asiaticus' was below the detection limit in cultures that displayed high abundances of Bacillus cereus. Our findings offer strategies for developing effective axenic culture conditions and suggest that enrichment of the Bacillaceae family could serve as a paratransgenic approach to controlling citrus greening disease.


Enzyme Microb Technol


Molki B,Call DR,Ha PT,Omsland A,Gang DR,Lindemann SR,Killiny N,Beyenal H




Has Abstract


2020-12-01 00:00:00












  • Multiple lignin peroxidases of Phanerochaete chrysosporium INA-12.

    abstract::Nine proteins with lignin peroxidase activity were separated from cultures of Phanerochaete chrysosporium INA-12 in glycerol as carbon source and non-nitrogen limited. Four lignin peroxidase isozymes (4, 5, 8, 9) were purified and characterized. Although differences in kinetic parameters could be shown, antibody react...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Odier E,Delattre M

    更新日期:1990-06-01 00:00:00

  • Exploring Trichoderma and Aspergillus secretomes: Proteomics approaches for the identification of enzymes of biotechnological interest.

    abstract::Filamentous fungal secretomes comprise highly dynamic sets of proteins, including multiple carbohydrate active enzymes (CAZymes) which are able to hydrolyze plant biomass polysaccharides into products of biotechnological interest such as fermentable sugars. In recent years, proteomics has been used to identify and qua...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章,评审


    authors: Cologna NMD,Gómez-Mendoza DP,Zanoelo FF,Giannesi GC,Guimarães NCA,Moreira LRS,Filho EXF,Ricart CAO

    更新日期:2018-02-01 00:00:00

  • A thermophilic enzymatic cocktail for galactomannans degradation.

    abstract::The full utilization of hemicellulose sugars (pentose and exose) present in lignocellulosic material, is required for an efficient bio-based fuels and chemicals production. Two recombinant thermophilic enzymes, an endo-1,4-β-mannanase from Dictyoglomus turgidum (DturCelB) and an α-galactosidase from Thermus thermophil...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Aulitto M,Fusco FA,Fiorentino G,Bartolucci S,Contursi P,Limauro D

    更新日期:2018-04-01 00:00:00

  • Fast, affordable and eco-friendly enzyme kinetic method for the assay of α-ketoglutaric acid in medical product and sports supplements.

    abstract::A novel kinetic method was developed for the quantitation of α-ketoglutaric acid (AKG) in cardioplegic solution and athletic supplements. The assay relied on an enzymatic transamination of AKG and d-4-hydroxyphenylglycine to form 4-hydroxybenzoylformic acid and l-glutamic acid using d-phenylglycine aminotransferase. S...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Rojanarata T,Laiwattanapaisal W,Ngawhirunpat T,Opanasopit P,Worrawethanakul K,Chantadee T,Fuangwattana T

    更新日期:2018-09-01 00:00:00

  • Stability of a recombinant shuttle plasmid in Bacillus subtilis and Escherichia coli.

    abstract::The recombinant shuttle plasmid pCPPS-31, which confers carboxymethylcellulase (CMCase) production and neomycin resistance (Ner), was segregationally but not structurally unstable in both B. subtilis (50-60%) and Escherichia coli (80-90%) when grown in serial batch cultures for 72 h diluting 1:1,000 with fresh medium ...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Vyas VV,Gupta S,Sharma P

    更新日期:1994-03-01 00:00:00

  • Conversion of cysteine to 3-mercaptopyruvic acid by bacterial aminotransferases.

    abstract::3-Mercaptopyruvate (3MPy), a structural analog of 3-mercaptopropionic acid, is a precursor compound for biosynthesis of polythioesters in bacteria. The cost-effectiveness and sustainability of the whole process could be greatly improved by using the cysteine degradation pathway for an intracellular supply of 3MPy. Tra...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Andreeßen C,Gerlt V,Steinbüchel A

    更新日期:2017-04-01 00:00:00

  • Enzymatic synthesis of phenethyl ester from phenethyl alcohol with acyl donors.

    abstract::Phenethyl ester is an important contributors to food, drinks, cosmetics and perfumery. Phenethyl acetate was synthesized enzymatically from phenethyl alcohol with acyl donors catalyzed by Novozym 435 from Candida antarctica lipase B. Through acyl donor screening, acetic anhydride and vinyl acetate were found to be the...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Kim H,Park C

    更新日期:2017-05-01 00:00:00

  • Acyl transfer reactions catalyzed by native and modified alpha-chymotrypsin in acetonitrile with low water content.

    abstract::The characterization of the S' subsite specificity of native and ethylated alpha-chymotrypsin has been studied via acyl transfer reaction in acetonitrile containing 10 vol% of water. Using Ac-Tyr-OEt as acyl donor, we investigated the partitioning of acyl-chymotrypsins between water and amino acid and peptide-derived ...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Cerovský V,Jakubke HD

    更新日期:1994-07-01 00:00:00

  • Recovery of purified lactonic sophorolipids by spontaneous crystallization during the fermentation of sugarcane molasses with Candida albicans O-13-1.

    abstract::Numerous studies have focused on how to obtain high yield of sophorolipids using low-cost materials as substrates, and there has been various work on the experimental methods for purifying lactonic sophorolipids. These studies have not yet obtained satisfied results in combining a low-cost fermentation process and the...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Yang X,Zhu L,Xue C,Chen Y,Qu L,Lu W

    更新日期:2012-12-10 00:00:00

  • Biochemical stability and molecular dynamic characterization of Aspergillus fumigatus cystathionine γ-lyase in response to various reaction effectors.

    abstract::Cystathionine γ-lyase (CGL) is a key enzyme in the methionine-cysteine cycle in all living organisms forming cysteine, α-ketobutyrate and ammonia via homocysteine and cystathionine intermediates. Although, human and plant CGLs have been extensively studied at the molecular and mechanistic levels, there has been little...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: El-Sayed AS,Abdel-Azeim S,Ibrahim HM,Yassin MA,Abdel-Ghany SE,Esener S,Ali GS

    更新日期:2015-12-01 00:00:00

  • Course of pH during the formation of amoxicillin by a suspension-to-suspension reaction.

    abstract::Amoxicillin can be produced in an enzymatic suspension-to-suspension reaction in which the substrate(s) and product(s) are mainly present as solid particles, while the reaction takes place in the liquid phase. During these suspension-to-suspension reactions different subprocesses take place, such as dissolution/crysta...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Diender MB,Straathof AJ,van der Does T,Zomerdijk M,Heijnen JJ

    更新日期:2000-11-01 00:00:00

  • UV-Vis detection of hydrogen peroxide using horseradish peroxidase/copper phosphate hybrid nanoflowers.

    abstract::A colorimetric platform based on enzyme-inorganic hybrid nanoflowers was used for ultraviolet-visible detection of hydrogen peroxide (H2O2). The hybrid nanoflowers were prepared via incubation at room temperature using horseradish peroxidase (HRP) and copper phosphate (Cu3(PO4)2) as the organic and inorganic component...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Yang C,Zhang M,Wang W,Wang Y,Tang J

    更新日期:2020-10-01 00:00:00

  • New-to-nature sophorose analog: a potent inducer for gene expression in Trichoderma reesei.

    abstract::Controlled hydrolysis of lactonic sophorolipids from Starmerella bombicola yields a previously undescribed sophorose analog that potently induces cellulase in Trichoderma reesei Rut-C30. Acid treatment of natural sophorolipids results in a mixture of monoacetylated, deacetylated, and diacetylated sophorolipids in acid...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Huang TT,Wages JM

    更新日期:2016-04-01 00:00:00

  • An efficient purification process for sweet potato beta-amylase by affinity precipitation with alginate.

    abstract::beta-amylases are used in production of maltose syrup. It is shown that sweet potato beta-amylase can be purified by affinity precipitation with alginate with 80% activity yield and 44 fold purification. SDS-PAGE of the purified protein showed a single band and a subunit weight of 50 kDa. Preliminary data with soybean...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Teotia S,Khare SK,Gupta MN

    更新日期:2001-06-07 00:00:00

  • Factors relevant to the production of (R)-(+)-glycidol (2,3-epoxy-1-propanol) from racemic glycidol by enantioselective oxidation with Acetobacter pasteurianus ATCC 12874.

    abstract::Acetobacter pasteurianus oxidizes glycidol with high activity, comparable to the oxidation of ethanol. The organism has a preference for the S-enantiomer, and the kinetic resolution process obeys a simple relationship, indicating an enantiomeric ratio (E) of 19. The compound is converted into glycidic acid, although a...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Geerlof A,Jongejan JA,van Dooren TJ,Racemakers-Franken PC,van den Tweel WJ,Duine JA

    更新日期:1994-12-01 00:00:00

  • DNase activity of micrococcal endonuclease covalently immobilized on nylon and polystyrene.

    abstract::Water-insoluble nucleases were prepared by immobilizing the endonuclease from S. aureus onto the surface of nylon-66 and polystyrene spheres. The activation phase of the synthetic supports was optimized to define optimal conditions of pH, temperature, and Ca2+ concentration for using immobilized enzymes. The activity,...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Michalon P,Roche J,Couturier R,Favre-Bonvin G,Marion C

    更新日期:1993-03-01 00:00:00

  • Porous biochar/chitosan composites for high performance cellulase immobilization by glutaraldehyde.

    abstract::In this work, porous biochar derived from sugarcane bagasse was prepared and then coated with different amounts of chitosan (C@CS) for cellulase immobilization. Cellulase was covalently immobilized on the support by using glutaraldehyde as a linker. The chemical characteristics and morphology of the samples were deter...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Mo H,Qiu J,Yang C,Zang L,Sakai E,Chen J

    更新日期:2020-08-01 00:00:00

  • Progress in using threonine aldolases for preparative synthesis.

    abstract::Three threonine aldolases (TAs) were cloned and overexpressed in Escherichia coli (Aeromonas jandaeil-allo-threonine aldolase, E. colil-threonine aldolase and Thermotoga maritimal-allo-threonine aldolase). A Design of Experiments strategy was used to identify optimal reaction conditions for each enzyme. These conditio...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Beaudoin SF,Hanna MP,Ghiviriga I,Stewart JD

    更新日期:2018-12-01 00:00:00

  • A biosensor based on gold nanoparticles, dihexadecylphosphate, and tyrosinase for the determination of catechol in natural water.

    abstract::In this work, a biosensor using a glassy carbon electrode modified with gold nanoparticles (AuNPs) and tyrosinase (Tyr) within a dihexadecylphosphate film is proposed. Cystamine and glutaraldehyde crosslinking agents were used as a support for Tyr immobilization. The proposed biosensor was characterized by scanning el...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Campanhã Vicentini F,Garcia LL,Figueiredo-Filho LC,Janegitz BC,Fatibello-Filho O

    更新日期:2016-03-01 00:00:00

  • Magnetosomes extracted from Magnetospirillum magneticum strain AMB-1 showed enhanced peroxidase-like activity under visible-light irradiation.

    abstract::Magnetosomes are intracellular structures produced by magnetotactic bacteria and are magnetic nanoparticles surrounded by a lipid bilayer membrane. Magnetosomes reportedly possess intrinsic enzyme mimetic activity similar to that found in horseradish peroxidase (HRP) and can scavenge reactive oxygen species depending ...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Li K,Chen C,Chen C,Wang Y,Wei Z,Pan W,Song T

    更新日期:2015-05-01 00:00:00

  • Enhanced bioconversion rate and released substrate inhibition in (R)-phenylephrine whole-cell bioconversion via partial acetone treatment.

    abstract::An approach was developed to enhance the efficiency for the bioconversion of 1-(3-hydroxyphenyl)-2-(methyamino)-ethanone to (R)-phenylephrine. The strain Serratia marcescens N10612, giving the benefit of 99% enantiomeric excess in (R)-PE conversion, was used. The fermentation was devised to harvest cells with high hyd...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Kan SC,Zang CZ,Yeh CW,Chang WF,Lin CC,Hung TH,Shieh CJ,Liu YC

    更新日期:2016-05-01 00:00:00

  • Evaluation of the effects of isolated lignin on enzymatic hydrolysis of cellulose.

    abstract::The different physical and chemical properties of lignin might have various effects on the enzymatic hydrolysis of lignocellulosic substrates. In this study, the influence of lignin on enzymatic digestibility of cellulose was assessed. Addition of 20% (4g/L) isolated enzymatic lignin (lignin 2 and 3) and kraft lignin ...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Zhang H,Wu S,Xie J

    更新日期:2017-06-01 00:00:00

  • Improvement of catalytical properties of two invertases highly tolerant to sucrose after expression in Pichia pastoris. Effect of glycosylation on enzyme properties.

    abstract::Zymomonas mobilis genes encoding INVA and INVB were expressed in Pichia pastoris, under the control of the strong AOX1 promoter, and the recombinant enzymes were named INVAAOX1 and INVBAOX1. The expression levels of INVAAOX1 (1660 U/mg) and INVBAOX1 (1993 U/mg) in P. pastoris were 9- and 7-fold higher than those obser...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Pérez de los Santos AI,Cayetano-Cruz M,Gutiérrez-Antón M,Santiago-Hernández A,Plascencia-Espinosa M,Farrés A,Hidalgo-Lara ME

    更新日期:2016-02-01 00:00:00

  • Comments on "Bioprocessing in space"

    abstract::An analysis developed by Westgate et al. for the digestible energy of edible and inedible biomass, including hydrolysis and fermentation, is reexamined with state-of-the-art values for the harvest index of hydroponic crops. ...

    journal_title:Enzyme and microbial technology

    pub_type: 评论,杂志文章


    authors: Volk T

    更新日期:1993-10-01 00:00:00

  • Characterization and solvent engineering of wheat β-amylase for enhancing its activity and stability.

    abstract::The kinetic and thermodynamic parameters of wheat β-amylase (WBA) were characterized and various additives were evaluated for enhancing its activity and thermostability. WBA activity was examined by neocuproine method using soluble starch as substrate. The Michaelis constant (K(m)) and molecular activity (k(cat)) were...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Daba T,Kojima K,Inouye K

    更新日期:2012-10-10 00:00:00

  • Increasing the refolding efficiency in vitro by site-directed mutagenesis of Cys383 in rat procarboxypeptidase B.

    abstract::This study examines a novel method to reduce the probability of disulfide mismatches during the refolding process by the replacement of cysteines within a protein. Specifically, Cys383 of recombinant rat procarboxypeptidase B was replaced by other amino acids to increase the refolding efficiency in vitro. Mutants C383...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Li S,Zhang L,Wu Q,Xin A,Zhao J,Fan L

    更新日期:2011-07-10 00:00:00

  • Engineering a high-performance, metagenomic-derived novel xylanase with improved soluble protein yield and thermostability.

    abstract::The novel termite gut metagenomic-derived GH11 xylanase gene xyl7 was expressed in Escherichia coli BL21, and the purified XYL7 enzyme exhibited high specific activity (6340U/mg) and broad pH active range of 5.5-10.0. Directed evolution was employed to enhance the thermostability of XYL7; two mutants (XYL7-TC and XYL7...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Qian C,Liu N,Yan X,Wang Q,Zhou Z,Wang Q

    更新日期:2015-03-01 00:00:00

  • Trypsin inactivation by latex fabricated gold nanoparticles: A new strategy towards insect control.

    abstract::Before applying nanotechnologies in biomedical and environmental areas it is advised to study interactions of nanoparticles and other nanomaterials with biomacromolecule present in living system. Moreover there is scarcity of reports on interactions between nanoparticles and biomaterials. In present report a rapid, ec...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Patil CD,Borase HP,Suryawanshi RK,Patil SV

    更新日期:2016-10-01 00:00:00

  • A new strategy for strain improvement of Aurantiochytrium sp. based on heavy-ions mutagenesis and synergistic effects of cold stress and inhibitors of enoyl-ACP reductase.

    abstract::Developing a strain with high docosahexaenoic acid (DHA) yield and stable fermenting-performance is an imperative way to improve DHA production using Aurantiochytrium sp., a microorganism with two fatty acid synthesis pathways: polyketide synthase (PKS) pathway and Type I fatty acid synthase (FAS) pathway. This study ...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Cheng YR,Sun ZJ,Cui GZ,Song X,Cui Q

    更新日期:2016-11-01 00:00:00

  • Covalent immobilization of a flavoprotein monooxygenase via its flavin cofactor.

    abstract::A generic approach for flavoenzyme immobilization was developed in which the flavin cofactor is used for anchoring enzymes onto the carrier. It exploits the tight binding of flavin cofactors to their target apo proteins. The method was tested for phenylacetone monooxygenase (PAMO) which is a well-studied and industria...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Krzek M,van Beek HL,Permentier HP,Bischoff R,Fraaije MW

    更新日期:2016-01-01 00:00:00