Characterization of two acidic β-glucosidases and ethanol fermentation in the brown rot fungus Fomitopsis palustris.

Abstract:

:Two acidic β-glucosidases (βGI and βGII) from the brown rot fungus Fomitopsis palustris were purified to homogeneity by several chromatographic steps. βGI and βGII had molecular weights of 130 and 213 kDa, respectively, and exhibited optimum activity at pH 2.5 and 55°C. The K(m) values of βGI and βGII for p-nitrophenyl-β-d-glucopyranoside were 0.706 and 0.971 mM, respectively. Although the effect of metal ions and inhibitors differed between the two enzymes, both β-glucosidases exhibited preferential glucose release during hydrolysis of cello-oligosaccharides, indicating that βGI and βGII possess effective exo-type activities. Notably, F. palustris was able to produce ethanol when cultured on medium containing 20 g/l of glucose, mannose, cellobiose, and maltose, in which the maximum ethanol concentrations measured were 9.2, 8.7, 9.0, and 8.9 g/l, corresponding to 90.2%, 85.3%, 88.2%, and 87.3% of the theoretical yield, respectively. These findings suggest that F. palustris has the ability not only to secrete β-glucosidase enzymes effective at low pH, but also to function as a biocatalyst, which may be suitable for the conversion of lignocellulosic materials into ethanol.

journal_name

Enzyme Microb Technol

authors

Okamoto K,Sugita Y,Nishikori N,Nitta Y,Yanase H

doi

10.1016/j.enzmictec.2010.12.012

subject

Has Abstract

pub_date

2011-04-07 00:00:00

pages

359-64

issue

4-5

eissn

0141-0229

issn

1879-0909

pii

S0141-0229(10)00286-3

journal_volume

48

pub_type

杂志文章
  • Increase in the thermostability of GH11 xylanase XynJ from Bacillus sp. strain 41M-1 using site saturation mutagenesis.

    abstract::GH11 xylanase XynJ from Bacillus sp. strain 41M-1 has a β-jellyroll fold composed of eight β strands with a deep active-site cleft. We hypothesized that the thermostability of XynJ will increase if the flexibility of the β strands in the jellyroll structure is decreased without impairing activity. To verify this hypot...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章

    doi:10.1016/j.enzmictec.2019.109363

    authors: Takita T,Nakatani K,Katano Y,Suzuki M,Kojima K,Saka N,Mikami B,Yatsunami R,Nakamura S,Yasukawa K

    更新日期:2019-11-01 00:00:00

  • Enhanced lipase-catalyzed synthesis of sugar fatty acid esters using supersaturated sugar solution in ionic liquids.

    abstract::A solvent-mediated method (SMM) was used to prepare supersaturated sugar solutions in hydrophobic and mixture of hydrophilic/hydrophobic ionic liquids (ILs), namely, [Bmim][Tf2N] and [Bmim][TfO]/[Bmim][Tf2N], respectively. In this method, sugars were first solubilized in a mixture of organic solvent and water (i.e. me...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章

    doi:10.1016/j.enzmictec.2019.03.004

    authors: Shin DW,Mai NL,Bae SW,Koo YM

    更新日期:2019-07-01 00:00:00

  • Kinetic study on anaerobic oxidation of methane coupled to denitrification.

    abstract::Monod kinetic parameters provide information required for kinetic analysis of anaerobic oxidation of methane coupled to denitrification (AOM-D). This information is critical for engineering AOM-D processes in wastewater treatment facilities. We first experimentally determined Monod kinetic parameters for an AOM-D enri...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章

    doi:10.1016/j.enzmictec.2017.05.005

    authors: Yu H,Kashima H,Regan JM,Hussain A,Elbeshbishy E,Lee HS

    更新日期:2017-09-01 00:00:00

  • Purification and functional characterization of the first stilbene glucoside-specific β-glucosidase isolated from Lactobacillus kimchi.

    abstract::This study aimed to develop viable enzymes for bioconversion of resveratrol-glucoside into resveratrol. Out of 13 bacterial strains tested, Lactobacillus kimchi JB301 could completely convert polydatin into resveratrol. The purified enzyme had an optimum temperature of 30-40°C and optimum pH of pH 5.0 against polydati...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章

    doi:10.1016/j.enzmictec.2014.09.001

    authors: Ko JA,Park JY,Kwon HJ,Ryu YB,Jeong HJ,Park SJ,Kim CY,Oh HM,Park CS,Lim YH,Kim D,Rho MC,Lee WS,Kim YM

    更新日期:2014-12-01 00:00:00

  • Identification, repair and characterization of a benzyl alcohol-inducible promoter for recombinant proteins overexpression in Corynebacterium glutamicum.

    abstract::Corynebacterium glutamicum is an important industrial organism for the production of a variety of biological commodities. We discovered a promoter encoded by the gene NCgl2319 in C. glutamicum, which could be induced by benzyl alcohol, could be used as an efficient tunable expression system. In initial attempts, this ...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章

    doi:10.1016/j.enzmictec.2020.109651

    authors: Liu X,Zhao Z,Dong G,Li Y,Peng F,Liu C,Zhang F,Linhardt RJ,Yang Y,Bai Z

    更新日期:2020-11-01 00:00:00

  • Enzymatic synthesis of cephalothin by penicillin G acylase*

    abstract::Enzymatic synthesis of cephalothin from 7-aminocephalosporanic acid (7-ACA) and amide derivatives of 2-thienylacetic acid (2-TA) using penicillin G acylase (pen G acylase) was studied. Two amide derivatives of 2-TA namely 2-thienylacetamide (2-TAA) and 2-thienylacetohydroxamic acid (2-TAH) were used in this study. The...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章

    doi:10.1016/s0141-0229(99)00153-2

    authors: Shaw S,Shyu J,Hsieh Y,Yeh H

    更新日期:2000-02-01 00:00:00

  • Measurement of hybridoma cell number, viability, and morphology using fully automated image analysis.

    abstract::A novel automated image analysis method is described for characterizing the viability and morphology of animal cells from suspension cultures. With the aid of the exclusion dye Trypan Blue, the total and viable cell counts and the percentage of dead cells present are found. The area, perimeter, equivalent diameter, an...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章

    doi:10.1016/0141-0229(94)90106-6

    authors: Tucker KG,Chalder S,al-Rubeai M,Thomas CR

    更新日期:1994-01-01 00:00:00

  • Identification of key sites determining the cofactor specificity and improvement of catalytic activity of a steroid 5β-reductase from Capsella rubella.

    abstract::Progesterone 5β-reductases (P5βRs) are involved in 5β-cardenolide formation by stereo-specific reduction of the △4,5 double bond of steroid precursors. In this study a steroid 5β-reductase was identified in Capsella rubella (CrSt5βR1) and its function in steroid 5β-reduction was validated experimentally. CrSt5βR1 is c...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章

    doi:10.1016/j.enzmictec.2019.109483

    authors: Li Y,Pan H,Chang Y,Dong N,Zou L,Liang P,Tian W,Chang Z

    更新日期:2020-03-01 00:00:00

  • Structural characterization and biological evaluation of Staphylosan (dimannooleate), a new glycolipid surfactant produced by a marine Staphylococcus saprophyticus SBPS-15.

    abstract::Marine microbes have gained significant attention as potential biofactories for broad spectrum bioactive compounds. In the recent years, bioactive biosurfactants have warranted renewed interest from both environmental and clinical sectors as anti-biofilm agents due to their excellent properties of dispersing microbial...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章

    doi:10.1016/j.enzmictec.2018.09.008

    authors: Balan SS,Mani P,Kumar CG,Jayalakshmi S

    更新日期:2019-01-01 00:00:00

  • Kinetic resolution of sec-alcohols catalysed by Candida antarctica lipase B displaying Pichia pastoris whole-cell biocatalyst.

    abstract::Kinetic resolution of sec-alcohols is a green process with biocatalyst. Candida antarctica lipase B (CALB) displayed on Pichia pastoris cell-surface (Pp-CALB) was characterized in kinetic resolution of sec-alcohols with different structures. The reaction parameters including acyl donors, molar ratio of substrates, sol...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章

    doi:10.1016/j.enzmictec.2017.11.005

    authors: Zhang K,Pan Z,Diao Z,Liang S,Han S,Zheng S,Lin Y

    更新日期:2018-03-01 00:00:00

  • Effect of threonine, cystathionine, and the branched-chain amino acids on the metabolism of Zygosaccharomyces rouxii*

    abstract::Zygosaccharomyces rouxii is an important yeast in the formation of flavor in soy sauce. In this study, we investigated the separate effects of exogenous threonine, cystathionine, and the branched-chain amino acids on the metabolism of Z. rouxii. The addition of these amino acids had significant effects on both Z. roux...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章

    doi:10.1016/s0141-0229(99)00165-9

    authors: van der Sluis C,Wolken WA,Giuseppin ML,Tramper J,Wijffels RH

    更新日期:2000-02-01 00:00:00

  • Preparation of high activity whole cell biocatalyst by permeabilization of recombinant flocculent yeast with alcohol.

    abstract::Flocculent yeast Saccharomyces cerevisiae YF234 (MATa ura3-52 trp1Delta2 his ade 2-1 can1-100 sta1 FLO8) cells overexpressing glyoxalase I and having strong flocculation ability were permeabilized with isopropyl alcohol and ethanol under various conditions. The treatment with 40% isopropyl alcohol significantly improv...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章

    doi:10.1016/s0141-0229(00)00304-5

    authors: Kondo A,Liu Y,Furuta M,Fujita Y,Matsumoto T,Fukuda H

    更新日期:2000-12-01 00:00:00

  • Magnetosomes extracted from Magnetospirillum magneticum strain AMB-1 showed enhanced peroxidase-like activity under visible-light irradiation.

    abstract::Magnetosomes are intracellular structures produced by magnetotactic bacteria and are magnetic nanoparticles surrounded by a lipid bilayer membrane. Magnetosomes reportedly possess intrinsic enzyme mimetic activity similar to that found in horseradish peroxidase (HRP) and can scavenge reactive oxygen species depending ...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章

    doi:10.1016/j.enzmictec.2015.02.009

    authors: Li K,Chen C,Chen C,Wang Y,Wei Z,Pan W,Song T

    更新日期:2015-05-01 00:00:00

  • Studies on the purification of peroxidase from horseradish roots using reverse micelles.

    abstract::Horseradish peroxidase (HRP) was successfully purified from horseradish roots by a two-stage reverse-micellar extraction from the dialyzed aqueous extract. The anionic surfactant AOT dissolved in isooctane was used to produce the reverse-micellar phases. The narrow pH range at which HRP solubilization occurred was exp...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章

    doi:10.1016/0141-0229(95)00031-3

    authors: Regalado C,Asenjo JA,Pyle DL

    更新日期:1996-04-01 00:00:00

  • Overexpression of NAD kinases improves the L-isoleucine biosynthesis in Corynebacterium glutamicum ssp. lactofermentum.

    abstract::NADPH is the key cofactor in L-isoleucine (Ile) biosynthetic pathway. To increase the Ile biosynthesis in Corynebacterium glutamicum ssp. lactofermentum JHI3-156, NADPH supply needs to be enhanced. Here NAD kinase, the key enzyme for the de novo biosynthesis of NADP(+) and NADPH, were cloned and expressed in JHI3-156,...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章

    doi:10.1016/j.enzmictec.2012.04.003

    authors: Shi F,Huan X,Wang X,Ning J

    更新日期:2012-07-15 00:00:00

  • Characterization of a family B DNA polymerase from Thermococcus barophilus Ch5 and its application for long and accurate PCR.

    abstract::The family B DNA polymerase gene from the euryarchaeon Thermococcus barophilus Ch5 (Tba5) contains an open reading frame of 6198 base pairs that encodes 2065 amino acid residues. The gene is split by three inteins that must be spliced out to form the mature DNA polymerase. A Tba5 DNA polymerase gene without inteins (g...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章

    doi:10.1016/j.enzmictec.2016.02.004

    authors: Kwon KM,Kang SG,Sokolova TG,Cho SS,Kim YJ,Kim CH,Kwon ST

    更新日期:2016-05-01 00:00:00

  • Screening of microbes for novel acidic cutinases and cloning and expression of an acidic cutinase from Aspergillus niger CBS 513.88.

    abstract::Isolates from gardening waste compost and 38 culture collection microbes were grown on agar plates at pH 4.0 with the cutinase model substrate polycaprolactone as a carbon source. The strains showing polycaprolactone hydrolysis were cultivated in liquid at acidic pH and the cultivations were monitored by assaying the ...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章

    doi:10.1016/j.enzmictec.2013.01.005

    authors: Nyyssölä A,Pihlajaniemi V,Järvinen R,Mikander S,Kontkanen H,Kruus K,Kallio H,Buchert J

    更新日期:2013-04-10 00:00:00

  • Engineering the central pathways in Lactococcus lactis: functional expression of the phosphofructokinase (pfk) and alternative oxidase (aox1) genes from Aspergillus niger in Lactococcus lactis facilitates improved carbon conversion rates under oxidizing c

    abstract::The present work describes a novel central pathway engineering method that has been designed with the aim to increase the carbon conversion rates under oxidizing conditions in L. lactis fermentations. The nisin producer L. lactis ATCC11454 strain has been genetically engineered by cloning a truncated version of the ph...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章

    doi:10.1016/j.enzmictec.2012.04.007

    authors: Papagianni M,Avramidis N

    更新日期:2012-08-10 00:00:00

  • ZnS quantum dots as pH probes for study of enzyme reaction kinetics.

    abstract::Water soluble ZnS quantum dots (QDs) modified by mercaptoacetic acid (MAA) were used to determinate proton concentration in aqueous solutions by fluorescence spectroscopic technique. The results showed that the fluorescence of the water-soluble QDs could be quenched by proton concentration and the fluorescence intensi...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章

    doi:10.1016/j.enzmictec.2012.03.012

    authors: Wu D,Chen Z

    更新日期:2012-06-10 00:00:00

  • An efficient in vitro refolding of recombinant bacterial laccase in Escherichia coli.

    abstract::Laccases (benzenediol oxygen oxidoreductases, EC 1.10.3.2) are important multicopper enzymes that are used in many biotechnological processes. A recombinant form of laccase from Bacillus sp. HR03 was overexpressed in Escherichia coli BL-21(DE3). Inclusion body (IB) formation happens quite often during recombinant prot...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章

    doi:10.1016/j.enzmictec.2013.03.006

    authors: Mollania N,Khajeh K,Ranjbar B,Rashno F,Akbari N,Fathi-Roudsari M

    更新日期:2013-05-10 00:00:00

  • Trypsin inactivation by latex fabricated gold nanoparticles: A new strategy towards insect control.

    abstract::Before applying nanotechnologies in biomedical and environmental areas it is advised to study interactions of nanoparticles and other nanomaterials with biomacromolecule present in living system. Moreover there is scarcity of reports on interactions between nanoparticles and biomaterials. In present report a rapid, ec...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章

    doi:10.1016/j.enzmictec.2016.06.005

    authors: Patil CD,Borase HP,Suryawanshi RK,Patil SV

    更新日期:2016-10-01 00:00:00

  • The effect of a lytic polysaccharide monooxygenase and a xylanase from Gloeophyllum trabeum on the enzymatic hydrolysis of lignocellulosic residues using a commercial cellulase.

    abstract::Hydrolysis of lignocellulosic biomass depends on the concerted actions of cellulases and accessory proteins. In this work we examined the combined action of two auxiliary proteins from the brown rot fungus Gloeophyllum trabeum: a family AA9 lytic polysaccharide monooxygenase (GtLPMO) and a GH10 xylanase (GtXyn10A). Th...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章

    doi:10.1016/j.enzmictec.2017.11.007

    authors: Sanhueza C,Carvajal G,Soto-Aguilar J,Lienqueo ME,Salazar O

    更新日期:2018-06-01 00:00:00

  • Structural change in wood by brown rot fungi and effect on enzymatic hydrolysis.

    abstract::The effects of biological pretreatment on Pinus radiata and Eucalyptus globulus, were evaluated after exposure to two brown rot fungi Gloephylum trabeum and Laetoporeus sulphureus. Changes in chemical composition, structural modification, and susceptibility to enzymatic hydrolysis in the degraded wood were analyzed. A...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章

    doi:10.1016/j.enzmictec.2011.08.004

    authors: Monrroy M,Ortega I,Ramírez M,Baeza J,Freer J

    更新日期:2011-10-10 00:00:00

  • Enzymatic production of cephalexin.

    abstract::Enzymatic production of cephalexin using immobilized penicillin G acylase was studied. The factors that affect the conversion of 7-amino-3-deacetoxycephalosporanic acid (7-ADCA) to cephalexin, such as pH; temperature; concentrations of 7-ADCA, D(-)-phenylglycine methyl ester (PGME), and immobilized penicillin G acylas...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章

    doi:10.1016/0141-0229(94)90095-7

    authors: Maladkar NK

    更新日期:1994-08-01 00:00:00

  • Construction of glutamate biosensor based on covalent immobilization of glutamate oxidase on polypyrrole nanoparticles/polyaniline modified gold electrode.

    abstract::A method is described for construction of a highly sensitive electrochemical biosensor for detection of glutamate. The biosensor is based on covalent immobilization of glutamate oxidase (GluOx) onto polypyrrole nanoparticles and polyaniline composite film (PPyNPs/PANI) electrodeposited onto Au electrode. The enzyme el...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章

    doi:10.1016/j.enzmictec.2014.02.001

    authors: Batra B,Kumari S,Pundir CS

    更新日期:2014-04-10 00:00:00

  • Enzymatic production of glycerol acetate from glycerol.

    abstract::In this study, we report the enzymatic production of glycerol acetate from glycerol and methyl acetate. Lipases are essential for the catalysis of this reaction. To find the optimum conditions for glycerol acetate production, sequential experiments were designed. Type of lipase, lipase concentration, molar ratio of re...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章

    doi:10.1016/j.enzmictec.2014.11.004

    authors: Oh S,Park C

    更新日期:2015-02-01 00:00:00

  • Functional analysis of active amino acid residues of the mercaptosuccinate dioxygenase of Variovorax paradoxus B4.

    abstract::Thiol dioxygenases are non-heme mononuclear-iron proteins and belong to the cupin superfamily. In 2014, mercaptosuccinate dioxygenase (Msdo) of Variovorax paradoxus B4 was identified as another bacterial cysteine dioxygenase (Cdo) homolog catalyzing the conversion of mercaptosuccinate (MS) into succinate and sulfite. ...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章

    doi:10.1016/j.enzmictec.2018.09.007

    authors: Brandt U,Galant G,Meinert-Berning C,Steinbüchel A

    更新日期:2019-01-01 00:00:00

  • A novel cold-adapted phospholipase A(1) from Serratia sp. xjF1: Gene cloning, expression and characterization.

    abstract::The gene encoding a cold-adapted phospholipase A(1) (PLA(1)) from a psychrotrophic, glacier soil bacterium Serratia sp. xjF1 was cloned by two-step PCR (general PCR and TAIL-PCR). The full-length fragment comprised two open reading frames plA and plS. The gene product of plA encoding 320 amino acids with a molecular w...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章

    doi:10.1016/j.enzmictec.2007.09.004

    authors: Fu J,Huang H,Meng K,Yuan T,Yao B,Shi Y,Ouyang P

    更新日期:2008-01-01 00:00:00

  • Fast, affordable and eco-friendly enzyme kinetic method for the assay of α-ketoglutaric acid in medical product and sports supplements.

    abstract::A novel kinetic method was developed for the quantitation of α-ketoglutaric acid (AKG) in cardioplegic solution and athletic supplements. The assay relied on an enzymatic transamination of AKG and d-4-hydroxyphenylglycine to form 4-hydroxybenzoylformic acid and l-glutamic acid using d-phenylglycine aminotransferase. S...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章

    doi:10.1016/j.enzmictec.2018.05.013

    authors: Rojanarata T,Laiwattanapaisal W,Ngawhirunpat T,Opanasopit P,Worrawethanakul K,Chantadee T,Fuangwattana T

    更新日期:2018-09-01 00:00:00

  • Kinetics of sequential nitrification and denitrification processes.

    abstract::Kinetics of nitrification and denitrification of synthetic wastewater was investigated by using two reactors in series. An activated sludge unit was used for nitrification followed by a downflow biofilter for denitrification. Glucose solution was fed to the denitrification column to supply carbon source. Reactors were...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章

    doi:10.1016/s0141-0229(00)00145-9

    authors: Dinçer AR,Kargi F

    更新日期:2000-07-01 00:00:00