当前位置： SCI文献检索 > ENZYME AND MICROBIAL TECHNOLOGY期刊下所有文献 > Stabilization of Penicillium occitanis cellulases by spray drying in presence of Maltodextrin.

Stabilization of Penicillium occitanis cellulases by spray drying in presence of Maltodextrin.

Abstract：

:The stabilization of fungal cellulases by spray drying, the thermal stability of Penicillium occitanis cellulases and the effect of some additives were studied. We observed that CMCase activity presents a good stability at 50 degrees C, even after 60 h of incubation. On the other hand, beta-glucosidase activity was more sensitive (loss of 50%) and reacts on total cellulases activities (Filter paper activities). The addition of hydrophilic agents such as ethylene glycol, polyethylene glycol (PEG6000) enhanced enzyme activity. The effect of PEG and Maltodextrin, another water activity decreasing agent, were then tested during the spray drying of Pol6 cellulases. The presence of 1% PEG allowed the best recovery but had a negative effect on enzyme stability while 1% Maltodextrin showed a negative effect on enzyme recovery but a very positive effect on enzyme stabilization.

journal_name

Enzyme Microb Technol

journal_title

Enzyme and microbial technology

authors

Belghith H,Ellouz Chaabouni S,Gargouri A

doi

10.1016/s0141-0229(00)00322-7

keywords:

subject

Has Abstract

pub_date

2001-02-01 00:00:00

pages

253-258

issue

2-3

eissn

0141-0229

issn

1879-0909

pii

S0141022900003227

journal_volume

28

pub_type

杂志文章

相关文献

ENZYME AND MICROBIAL TECHNOLOGY文献大全
  • A biosensor based on gold nanoparticles, dihexadecylphosphate, and tyrosinase for the determination of catechol in natural water.

    abstract：:In this work, a biosensor using a glassy carbon electrode modified with gold nanoparticles (AuNPs) and tyrosinase (Tyr) within a dihexadecylphosphate film is proposed. Cystamine and glutaraldehyde crosslinking agents were used as a support for Tyr immobilization. The proposed biosensor was characterized by scanning el...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2015.12.004

    authors： Campanhã Vicentini F,Garcia LL,Figueiredo-Filho LC,Janegitz BC,Fatibello-Filho O

    更新日期：2016-03-01 00:00:00

  • The use of oxygen uptake rate measurements to control the supply of toxic substrate: toluene hydroxylation by Pseudomonas putida UV4.

    abstract：:During toluene hydroxylation, catalyzed by Pseudomonas putida UV4 one molecule of oxygen is added to the aromatic ring to produce the dihydroxylated (non-aromatic) ring structure, toluene cis-glycol. Toluene, which is toxic to the cells at aqueous phase concentration above ( approximately 2.4 mmol), is fed to the reac...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/s0141-0229(00)00289-1

    authors： Carragher JM,McClean WS,Woodley JM,Hack CJ

    更新日期：2001-02-01 00:00:00

  • Use of raw glycerol to produce oil rich in polyunsaturated fatty acids by a thraustochytrid.

    abstract：:Glucose is the typical carbon source for producing microbial polyunsaturated fatty acids (PUFA) with single cell microorganisms such as thraustochytrids. We assessed the use of a fish oil derived glycerol by-product (raw glycerol), produced by a fish oil processing plant, as a carbon source to produce single cell oil ...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2010.11.008

    authors： Scott SD,Armenta RE,Berryman KT,Norman AW

    更新日期：2011-03-07 00:00:00

  • A new strategy for strain improvement of Aurantiochytrium sp. based on heavy-ions mutagenesis and synergistic effects of cold stress and inhibitors of enoyl-ACP reductase.

    abstract：:Developing a strain with high docosahexaenoic acid (DHA) yield and stable fermenting-performance is an imperative way to improve DHA production using Aurantiochytrium sp., a microorganism with two fatty acid synthesis pathways: polyketide synthase (PKS) pathway and Type I fatty acid synthase (FAS) pathway. This study ...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2016.08.019

    authors： Cheng YR,Sun ZJ,Cui GZ,Song X,Cui Q

    更新日期：2016-11-01 00:00:00

  • A sialic acid aldolase from Peptoclostridium difficile NAP08 with 4-hydroxy-2-oxo-pentanoate aldolase activity.

    abstract：:Sialic acid aldolases (E.C.4.1.3.3) catalyze the reversible aldol cleavage of N-acetyl-d-neuraminic acid (Neu5Ac) to from N-acetyl-d-mannosamine (ManNAc) and pyruvate. In this study, a sialic acid aldolase (PdNAL) from Peptoclostridium difficile NAP08 was expressed in Escherichia coli BL21 (DE3). This homotetrameric e...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2016.07.003

    authors： Chen Q,Han L,Chen X,Cui Y,Feng J,Wu Q,Zhu D

    更新日期：2016-10-01 00:00:00

  • Structural change in wood by brown rot fungi and effect on enzymatic hydrolysis.

    abstract：:The effects of biological pretreatment on Pinus radiata and Eucalyptus globulus, were evaluated after exposure to two brown rot fungi Gloephylum trabeum and Laetoporeus sulphureus. Changes in chemical composition, structural modification, and susceptibility to enzymatic hydrolysis in the degraded wood were analyzed. A...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2011.08.004

    authors： Monrroy M,Ortega I,Ramírez M,Baeza J,Freer J

    更新日期：2011-10-10 00:00:00

  • Engineering the central pathways in Lactococcus lactis: functional expression of the phosphofructokinase (pfk) and alternative oxidase (aox1) genes from Aspergillus niger in Lactococcus lactis facilitates improved carbon conversion rates under oxidizing c

    abstract：:The present work describes a novel central pathway engineering method that has been designed with the aim to increase the carbon conversion rates under oxidizing conditions in L. lactis fermentations. The nisin producer L. lactis ATCC11454 strain has been genetically engineered by cloning a truncated version of the ph...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2012.04.007

    authors： Papagianni M,Avramidis N

    更新日期：2012-08-10 00:00:00

  • Multi-functional glycoside hydrolase: Blon_0625 from Bifidobacterium longum subsp. infantis ATCC 15697.

    abstract：:We here describe a unique β-D-glucosidase (BGL; Blon_0625) derived from Bifidobacterium longum subsp. infantis ATCC 15697. The Blon_0625 gene was expressed by recombinant Escherichia coli. Purified recombinant Blon_0625 retains hydrolyzing activity against both p-nitrophenyl-β-D-glucopyranoside (pNPG; 17.3±0.24Umg(-1)...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2014.10.001

    authors： Matsumoto T,Shimada S,Hata Y,Tanaka T,Kondo A

    更新日期：2015-01-01 00:00:00

  • Microbial surface displaying formate dehydrogenase and its application in optical detection of formate.

    abstract：:In the present work, NAD(+)-dependent formate dehydrogenase (FDH), encoded by fdh gene from Candida boidinii was successfully displayed on Escherichia coli cell surface using ice nucleation protein (INP) from Pseudomonas borealis DL7 as an anchoring protein. Localization of matlose binding protein (MBP)-INP-FDH fusion...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2016.06.002

    authors： Liu A,Feng R,Liang B

    更新日期：2016-09-01 00:00:00

  • Transient behavior of T-cell hybridomas in response to changes in metabolite concentrations in continuous culture.

    abstract：:Effects of pulse and step changes in major metabolite concentrations such as glucose, glutamine, lactic acid, and ammonium on behavior of T-cell hybridomas in continuous culture were investigated in order to develop a strategy to maximize specific rate of lymphokine formation. Step increases in glucose, lactic acid, a...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/0141-0229(90)90007-d

    authors： Shaughnessy TS,Kargi F

    更新日期：1990-09-01 00:00:00

  • A colorimetric method to quantify endo-polygalacturonase activity.

    abstract：:We report a new colorimetric assay to quantify endo-polygalacturonase activity, which hydrolyzes polygalacturonic acid to produce smaller chains of galacturonate. Some of the reported polygalacturonase assays measure the activity by detecting the appearance of reducing ends such as the Somogyi-Nelson method. As a resu...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2010.04.004

    authors： Torres S,Sayago JE,Ordoñez RM,Isla MI

    更新日期：2011-02-08 00:00:00

  • Antibacterial activity of emulsions containing unsaturated fatty acid ergosterol esters synthesized by lipase-mediated transesterification.

    abstract：:Ergosterol, like cholesterol, has many beneficial physiological activities, and because it has no adverse clinical problem with cholesterol, it is a substance that can be used as a biomaterial in the food and cosmetics industry. However, ergosterol has low oil solubility and is easily crystallized, which is problemati...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2020.109581

    authors： Park SH,Kim HK

    更新日期：2020-09-01 00:00:00

  • Multiple lignin peroxidases of Phanerochaete chrysosporium INA-12.

    abstract：:Nine proteins with lignin peroxidase activity were separated from cultures of Phanerochaete chrysosporium INA-12 in glycerol as carbon source and non-nitrogen limited. Four lignin peroxidase isozymes (4, 5, 8, 9) were purified and characterized. Although differences in kinetic parameters could be shown, antibody react...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/0141-0229(90)90056-v

    authors： Odier E,Delattre M

    更新日期：1990-06-01 00:00:00

  • The effect of a lytic polysaccharide monooxygenase and a xylanase from Gloeophyllum trabeum on the enzymatic hydrolysis of lignocellulosic residues using a commercial cellulase.

    abstract：:Hydrolysis of lignocellulosic biomass depends on the concerted actions of cellulases and accessory proteins. In this work we examined the combined action of two auxiliary proteins from the brown rot fungus Gloeophyllum trabeum: a family AA9 lytic polysaccharide monooxygenase (GtLPMO) and a GH10 xylanase (GtXyn10A). Th...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2017.11.007

    authors： Sanhueza C,Carvajal G,Soto-Aguilar J,Lienqueo ME,Salazar O

    更新日期：2018-06-01 00:00:00

  • Magnetosomes extracted from Magnetospirillum magneticum strain AMB-1 showed enhanced peroxidase-like activity under visible-light irradiation.

    abstract：:Magnetosomes are intracellular structures produced by magnetotactic bacteria and are magnetic nanoparticles surrounded by a lipid bilayer membrane. Magnetosomes reportedly possess intrinsic enzyme mimetic activity similar to that found in horseradish peroxidase (HRP) and can scavenge reactive oxygen species depending ...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2015.02.009

    authors： Li K,Chen C,Chen C,Wang Y,Wei Z,Pan W,Song T

    更新日期：2015-05-01 00:00:00

  • Characterization of two acidic β-glucosidases and ethanol fermentation in the brown rot fungus Fomitopsis palustris.

    abstract：:Two acidic β-glucosidases (βGI and βGII) from the brown rot fungus Fomitopsis palustris were purified to homogeneity by several chromatographic steps. βGI and βGII had molecular weights of 130 and 213 kDa, respectively, and exhibited optimum activity at pH 2.5 and 55°C. The K(m) values of βGI and βGII for p-nitropheny...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2010.12.012

    authors： Okamoto K,Sugita Y,Nishikori N,Nitta Y,Yanase H

    更新日期：2011-04-07 00:00:00

  • Eco-friendly intracellular biosynthesis of CdS quantum dots without changing Escherichia coli's antibiotic resistance.

    abstract：:In the paper, a green and efficient biosynthetical technique was reported for preparing cadmium sulfide (CdS) quantum dots, in which Escherichia coli (E. coli) was chosen as a biomatrix. Fluorescence emission spectra and fluorescent microscopic photographs revealed that as-produced CdS quantum dots had an optimum fluo...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2016.09.017

    authors： Yan ZY,Du QQ,Qian J,Wan DY,Wu SM

    更新日期：2017-01-01 00:00:00

  • Characterization of specificity of subtilisin Carlsberg towards peptide T by high-performance liquid chromatography and electrospray mass spectrometry.

    abstract：:Peptide T has a sequence (Ala-Ser-Thr-Thr-Thr-Asn-Tyr-Thr) belonging to HIV envelope that is involved in the interaction with CD(4) receptor of T lymphocytes. Research of protease activities towards this peptide is very relevant for AIDS therapy. Characterization of specificity of subtilisin Carlsberg towards this ver...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/s0141-0229(99)00170-2

    authors： Nedjar-Arroumea N,Paon M,Koralewski F,Friboulet A,Kouach M,Briand G,Leroy Y,Guillochon D

    更新日期：2000-03-01 00:00:00

  • RNA interference with carbon catabolite repression in Trichoderma koningii for enhancing cellulase production.

    abstract：:The cellulase and xylanase genes of filamentous Trichoderma fungi exist under carbon catabolite repression mediated by the regulator carbon catabolite repressor (CREI). Our objective was to find the role of CREI in a cellulase-hyperproducing mutant of Trichoderma koningii, and address whether enzyme production can be ...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2013.04.007

    authors： Wang S,Liu G,Yu J,Tian S,Huang B,Xing M

    更新日期：2013-07-10 00:00:00

  • Catalytic efficiency of a multi-domain transglycosylating chitinase from Enterobacter cloacae subsp. cloacae (EcChi2) is influenced by polycystic kidney disease domains.

    abstract：:Bacterial chitinases recruited multiple accessory domains for the conversion of recalcitrant polysaccharides to simple soluble sugars/amino sugars. Here, we report detailed properties of a multi-domain GH18 chitinase from Enterobacter cloacae subsp. cloacae (EcChi2) that preferred β-chitin as substrate. EcChi2 exhibit...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2020.109702

    authors： Mallakuntla MK,Podile AR

    更新日期：2021-02-01 00:00:00

  • Operational stabilities of different chemical derivatives of Novozym 435 in an alcoholysis reaction.

    abstract：:Industrial use of Novozym 435 in synthesis of structured lipids and biodiesel via alcoholysis is limited by mass transfer effects of the glycerides through immobilized enzymes and its low operational stability under operation conditions. To better understand this, differently modified Novozym 435 preparations, differi...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2016.04.007

    authors： Villalba M,Verdasco-Martín CM,Dos Santos JC,Fernandez-Lafuente R,Otero C

    更新日期：2016-08-01 00:00:00

  • Optimization and modelling of enzymatic polymerization of ε-caprolactone to polycaprolactone using Candida Antartica Lipase B with response surface methodology and artificial neural network.

    abstract：:Recently enzymatic catalysts have replaced organic and organometallic catalysts in the synthesis of bio-resorbable polymers. Enzymatic polymerization is considered as an alternative to conventional polymerization as they are less toxic, environmental friendly and can operate under mild conditions. In this research, th...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2018.12.001

    authors： Pakalapati H,Tariq MA,Arumugasamy SK

    更新日期：2019-03-01 00:00:00

  • Molecular insights into substrate specificity of Rhodococcus ruber CGMCC3090 by gene cloning and homology modeling.

    abstract：:The primary aim of this study was to decipher the catalytic functions of the NHase with wide substrate spectra from Rhodococcus ruber CGMCC3090 by computer modeling and substrate docking. 3D structure model of the enzyme was built by computer modeling to obtain the optimal structure. The larger binding site cavity (55...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2012.11.004

    authors： Wang S,Dai Y,Wang J,Shen Y,Zhai Y,Zheng H,Wang M

    更新日期：2013-02-05 00:00:00

  • Chemical modification of lysine residues in Bacillus alpha-amylases: effect on activity and stability.

    abstract：:Chemical modification of lysine residues in two bacterial alpha-amylases, a mesophilic enzyme from Bacillus amyloliquefaciens (BAA) and a thermophilic enzyme from Bacillus licheniformis (BLA) was carried out using citraconic anhydride. 13 +/- 1 residues in BAA and 10 +/- 1 residues in BLA were found modified under def...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/s0141-0229(01)00296-4

    authors： Khajeh K,Naderi-Manesh H,Ranjbar B,Moosavi-Movahedi A,Nemat-Gorgani M

    更新日期：2001-04-05 00:00:00

  • The effect of gas double-dynamic on mass distribution in solid-state fermentation.

    abstract：:The mass distribution regularity in substrate of solid-state fermentation (SSF) has rarely been reported due to the heterogeneity of solid medium and the lack of suitable instrument and method, which limited the comprehensive analysis and enhancement of the SSF performance. In this work, the distributions of water, bi...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2014.02.007

    authors： Chen HZ,Zhao ZM,Li HQ

    更新日期：2014-05-10 00:00:00

  • Overexpression of hlyB and mdh genes confers halotolerance in Fremyella diplosiphon, a freshwater cyanobacterium.

    abstract：:Fremyella diplosiphon is a freshwater cyanobacterium that has great potential as a biofuel agent due to its ability to grow in low light intensity and acclimation to different wavelengths. To enhance its halotolerance for growth in 35gL-1 sodium chloride (NaCl), plasmids harboring hemolysin B (hlyB) and malate dehydro...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2017.04.009

    authors： Tabatabai B,Arumanayagam AS,Enitan O,Mani A,Natarajan SS,Sitther V

    更新日期：2017-08-01 00:00:00

  • Effects of non-ionic surfactants on the interactions between cellulases and tannic acid: a model system for cellulase-poly-phenol interactions.

    abstract：:Addition of non-ionic surfactants (NIS) is known to accelerate enzymatic lignocellulose hydrolysis. The mechanism behind this accelerating effect is still not elucidated but has been hypothesized to originate from favorable NIS-lignin interactions which alleviate non-productive adsorption of cellulases to lignin. In t...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2011.06.015

    authors： Olsen SN,Bohlin C,Murphy L,Borch K,McFarland KC,Sweeny MD,Westh P

    更新日期：2011-09-10 00:00:00

  • Alginate as immobilization matrix and stabilizing agent in a two-phase liquid system: application in lipase-catalysed reactions.

    abstract：:Alginate was evaluated as an immobilization matrix for enzyme-catalyzed reactions in organic solvents. In contrast to most hydrogels, calcium alginate was found to be stable in a range of organic solvents and to retain the enzyme inside the gel matrix. In hydrophobic solvents, the alginate gel (greater than 95% water)...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/0141-0229(92)90024-i

    authors： Hertzberg S,Kvittingen L,Anthonsen T,Skjåk-Braek G

    更新日期：1992-01-01 00:00:00

  • Aspergillus niger I-1472 and Pycnoporus cinnabarinus MUCL39533, selected for the biotransformation of ferulic acid to vanillin, are also able to produce cell wall polysaccharide-degrading enzymes and feruloyl esterases.

    abstract：:The filamentous fungal strains Aspergillus niger I-1472 and Pycnoporus cinnabarinus MUCL39533, previously selected for the bioconversion of ferulic acid to vanillic acid and vanillin respectively, were grown on sugar beet pulp. A large spectrum of polysaccharide-degrading enzymes was produced by A. niger and very few ...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/s0141-0229(00)00277-5

    authors： Bonnina E,Brunel M,Gouy Y,Lesage-Meessen L,Asther M,Thibault J

    更新日期：2001-01-02 00:00:00

  • Effect of medium composition on the maintenance of a recombinant plasmid in Bacillus subtilis.

    abstract：:Recombinant plasmid pCED3 [confers beta-galactosidase production (LacZ+) and kanamycin resistance (Kmr)] in Bacillus subtilis was found to be both segregationally and structurally unstable. Since many solutions to segregational instability are already available, the problem of structural instability was specifically a...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/0141-0229(90)90160-r

    authors： Shoham Y,Demain AL

    更新日期：1990-05-01 00:00:00