Abstract：
:We report a new colorimetric assay to quantify endo-polygalacturonase activity, which hydrolyzes polygalacturonic acid to produce smaller chains of galacturonate. Some of the reported polygalacturonase assays measure the activity by detecting the appearance of reducing ends such as the Somogyi-Nelson method. As a result of being general towards reducing groups, the Somogyi-Nelson method is not appropriate when studying polygalacturonase and polygalacturonase inhibitors in plant crude extracts, which often have a strong reducing power. Ruthenium Red is an inorganic dye that binds polygalacturonic acid and causes its precipitation. In the presence of polygalacturonase, polygalacturonic acid is hydrolyzed bringing about a corresponding gain in soluble Ruthenium Red. The described assay utilizes Ruthenium Red as the detection reagent which has been used previously in plate-based assays but not in liquid medium reactions. The new method measures the disappearance of the substrate polygalacturonic acid and is compared to the Somogyi-Nelson assay. The experimental results using lemon peel, a fern fronds and castor leaf crude extracts demonstrate that the new method provides a way to the quickly screening of polygalacturonase activity and polygalacturonase inhibitors in plant crude extracts containing high amounts of reducing power. On the other hand, the Ruthenium Red assay is not able to determine the activity of an exo-polygalacturonase as initial velocity and thus would allow the differentiation between endo- and exo-polygalacturonase activities.
journal_name
Enzyme Microb Technoljournal_title
Enzyme and microbial technologyauthors
Torres S,Sayago JE,Ordoñez RM,Isla MIdoi
10.1016/j.enzmictec.2010.04.004subject
Has Abstractpub_date
2011-02-08 00:00:00pages
123-8issue
2eissn
0141-0229issn
1879-0909pii
S0141-0229(10)00071-2journal_volume
48pub_type
杂志文章abstract：:Supercritical fluids offer environmental advantages over chemical solvents, while providing enhanced separation and chemical selectivity. The use of supercritical fluids for the recovery of products from biomass and the transformation of selected molecules (to add value) was studied. Free fatty acids were bio-catalyti...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
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更新日期：2011-09-10 00:00:00
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journal_title：Enzyme and microbial technology
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journal_title：Enzyme and microbial technology
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journal_title：Enzyme and microbial technology
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journal_title：Enzyme and microbial technology
pub_type： 杂志文章
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更新日期：2013-02-05 00:00:00
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journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/j.enzmictec.2014.11.004
更新日期：2015-02-01 00:00:00
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更新日期：2010-05-05 00:00:00
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journal_title：Enzyme and microbial technology
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doi：10.1016/j.enzmictec.2015.11.008
更新日期：2016-02-01 00:00:00
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journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/j.enzmictec.2011.09.002
更新日期：2011-12-10 00:00:00
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journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/j.enzmictec.2011.04.001
更新日期：2011-06-10 00:00:00
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pub_type： 杂志文章
doi：10.1016/s0141-0229(00)00272-6
更新日期：2000-11-15 00:00:00
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journal_title：Enzyme and microbial technology
pub_type： 杂志文章
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journal_title：Enzyme and microbial technology
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journal_title：Enzyme and microbial technology
pub_type： 杂志文章
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journal_title：Enzyme and microbial technology
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journal_title：Enzyme and microbial technology
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journal_title：Enzyme and microbial technology
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journal_title：Enzyme and microbial technology
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更新日期：2020-12-01 00:00:00
abstract：:Di-, tri-, and tetra-tyrosine peptides with angiotensin I-converting enzyme inhibitory activity were synthesized by papain-catalyzed polymerization of L-tyrosine ethyl ester in aqueous media at 30 °C. Varying the reaction pH from 6.0 to 7.5 and the initial concentration of the ester substrate from 25 to 100 mM, the hi...
journal_title：Enzyme and microbial technology
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更新日期：2015-07-01 00:00:00
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journal_title：Enzyme and microbial technology
pub_type： 杂志文章,评审
doi：10.1016/0141-0229(93)90078-g
更新日期：1993-06-01 00:00:00
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journal_title：Enzyme and microbial technology
pub_type： 杂志文章
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更新日期：2013-01-10 00:00:00
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journal_title：Enzyme and microbial technology
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journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/j.enzmictec.2012.04.003
更新日期：2012-07-15 00:00:00
abstract：:Water-insoluble nucleases were prepared by immobilizing the endonuclease from S. aureus onto the surface of nylon-66 and polystyrene spheres. The activation phase of the synthetic supports was optimized to define optimal conditions of pH, temperature, and Ca2+ concentration for using immobilized enzymes. The activity,...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/0141-0229(93)90140-w
更新日期：1993-03-01 00:00:00
abstract：:An extracellular esterase from the actinomycetes Amycolatopsis orientalis was found by screening. It is capable of splitting the isomeric mixture (K/J) of (I, Scheme 1) into 7-amino-3-methoxymethyl-3-cephem-4-carboxylic acid, pivalic acid, and acetaldehyde with a high yield. The purified enzyme of 55.4 Kd by SDS-PAGE ...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/0141-0229(95)00171-9
更新日期：1996-07-01 00:00:00
