当前位置： SCI文献检索 > ENZYME AND MICROBIAL TECHNOLOGY期刊下所有文献 > Covalent immobilization of catalase onto spacer-arm attached modified florisil: characterization and application to batch and plug-flow type reactor systems.

Covalent immobilization of catalase onto spacer-arm attached modified florisil: characterization and application to batch and plug-flow type reactor systems.

Abstract：

:Catalase was covalently immobilized onto florisil via glutaraldehyde (GA) and glutaraldehyde+6-amino hexanoic acid (6-AHA) (as a spacer arm). Immobilizations of catalase onto modified supports were optimized to improve the efficiency of the overall immobilization procedures. The V(max) values of catalase immobilized via glutaraldehyde (CIG) and catalase immobilized via glutaraldehyde+6-amino hexanoic acid (CIG-6-AHA) were about 0.6 and 3.4% of free catalase, respectively. The usage of 6-AHA as a spacer arm caused about 40 folds increase in catalytic efficiency of CIG-6-AHA (8.3 × 10⁵ M⁻¹ s⁻¹) as compared to that of CIG (2.1 × 10⁴ M⁻¹ s⁻¹). CIG and CIG-6-AHA retained 67 and 35% of their initial activities at 5 °C and 71 and 18% of their initial activities, respectively at room temperature at the end of 6 days. Operational stabilities of CIG and CIG-6-AHA were investigated in batch and plug-flow type reactors. The highest total amount of decomposed hydrogen peroxide (TAD-H₂O₂) was determined as 219.5 μmol for CIG-6-AHA in plug-flow type reactor.

journal_name

Enzyme Microb Technol

journal_title

Enzyme and microbial technology

authors

Alptekin O,Tükel SS,Yildirim D,Alagöz D

doi

10.1016/j.enzmictec.2011.09.002

subject

Has Abstract

pub_date

2011-12-10 00:00:00

pages

547-54

issue

6-7

eissn

0141-0229

issn

1879-0909

pii

S0141-0229(11)00191-8

journal_volume

49

pub_type

杂志文章

相关文献

ENZYME AND MICROBIAL TECHNOLOGY文献大全
  • Multi-functional glycoside hydrolase: Blon_0625 from Bifidobacterium longum subsp. infantis ATCC 15697.

    abstract：:We here describe a unique β-D-glucosidase (BGL; Blon_0625) derived from Bifidobacterium longum subsp. infantis ATCC 15697. The Blon_0625 gene was expressed by recombinant Escherichia coli. Purified recombinant Blon_0625 retains hydrolyzing activity against both p-nitrophenyl-β-D-glucopyranoside (pNPG; 17.3±0.24Umg(-1)...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2014.10.001

    authors： Matsumoto T,Shimada S,Hata Y,Tanaka T,Kondo A

    更新日期：2015-01-01 00:00:00

  • A thermophilic enzymatic cocktail for galactomannans degradation.

    abstract：:The full utilization of hemicellulose sugars (pentose and exose) present in lignocellulosic material, is required for an efficient bio-based fuels and chemicals production. Two recombinant thermophilic enzymes, an endo-1,4-β-mannanase from Dictyoglomus turgidum (DturCelB) and an α-galactosidase from Thermus thermophil...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2017.12.008

    authors： Aulitto M,Fusco FA,Fiorentino G,Bartolucci S,Contursi P,Limauro D

    更新日期：2018-04-01 00:00:00

  • Characterization of a family B DNA polymerase from Thermococcus barophilus Ch5 and its application for long and accurate PCR.

    abstract：:The family B DNA polymerase gene from the euryarchaeon Thermococcus barophilus Ch5 (Tba5) contains an open reading frame of 6198 base pairs that encodes 2065 amino acid residues. The gene is split by three inteins that must be spliced out to form the mature DNA polymerase. A Tba5 DNA polymerase gene without inteins (g...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2016.02.004

    authors： Kwon KM,Kang SG,Sokolova TG,Cho SS,Kim YJ,Kim CH,Kwon ST

    更新日期：2016-05-01 00:00:00

  • Modulatory effect of low-shear modeled microgravity on stress resistance, membrane lipid composition, virulence, and relevant gene expression in the food-borne pathogen Listeria monocytogenes.

    abstract：:The present study investigated the influence of low-shear modeled microgravity (LSMMG) conditions on Listeria monocytogenes stress response (heat, cold, and acid), membrane fatty acid composition, and virulence potential as well as stress-/virulence-associated gene expression. The results showed that LSMMG-cultivated ...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2019.109440

    authors： Sheet S,Yesupatham S,Ghosh K,Choi MS,Shim KS,Lee YS

    更新日期：2020-02-01 00:00:00

  • Structural change in wood by brown rot fungi and effect on enzymatic hydrolysis.

    abstract：:The effects of biological pretreatment on Pinus radiata and Eucalyptus globulus, were evaluated after exposure to two brown rot fungi Gloephylum trabeum and Laetoporeus sulphureus. Changes in chemical composition, structural modification, and susceptibility to enzymatic hydrolysis in the degraded wood were analyzed. A...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2011.08.004

    authors： Monrroy M,Ortega I,Ramírez M,Baeza J,Freer J

    更新日期：2011-10-10 00:00:00

  • Effect of intense pulsed light on the deactivation of lipase: Enzyme-deactivation kinetics and tertiary structural changes by fragmentation.

    abstract：:The effect of intense pulsed light (IPL) irradiation on Chromobacterium viscosum lipase was investigated with a primary focus on catalytic activity and molecular structure. During IPL irradiation, lipase activity decreased significantly with increasing pulse fluence (Fp) and exposure time (te). IPL-induced deactivatio...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2019.02.001

    authors： Jeon MS,Park KM,Yu H,Park JY,Chang PS

    更新日期：2019-05-01 00:00:00

  • Electrochemical detection of Pseudomonas aeruginosa 16S rRNA using a biosensor based on immobilized stem-loop structured probe.

    abstract：:We have designed an electrochemical DNA biosensor based on stem-loop structured probes for enzymatic detection of Pseudomonas aeruginosa 16S ribosomal RNA (rRNA) in composting degradation. The probe modified with a thiol at its 5' end and a biotin at its 3' end was immobilized on a gold electrode through self-assembly...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2011.06.011

    authors： Liu C,Zeng GM,Tang L,Zhang Y,Li YP,Liu YY,Li Z,Wu MS,Luo J

    更新日期：2011-08-10 00:00:00

  • Chain length selectivity during the polycondensation of siloxane-containing esters and alcohols by immobilized Candida antarctica lipase B.

    abstract：:We have examined the chain length selectivity for a series of acyl donors by lipase B from Candida antarctica (CalB). CalB accepted aliphatic diesters of C4, C6 and C12 chain lengths equally. The introduction of a carbon-carbon double bond into the C4 esters dramatically lowered the rate constant associated with polym...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2014.03.006

    authors： Frampton MB,Zelisko PM

    更新日期：2014-05-10 00:00:00

  • A sialic acid aldolase from Peptoclostridium difficile NAP08 with 4-hydroxy-2-oxo-pentanoate aldolase activity.

    abstract：:Sialic acid aldolases (E.C.4.1.3.3) catalyze the reversible aldol cleavage of N-acetyl-d-neuraminic acid (Neu5Ac) to from N-acetyl-d-mannosamine (ManNAc) and pyruvate. In this study, a sialic acid aldolase (PdNAL) from Peptoclostridium difficile NAP08 was expressed in Escherichia coli BL21 (DE3). This homotetrameric e...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2016.07.003

    authors： Chen Q,Han L,Chen X,Cui Y,Feng J,Wu Q,Zhu D

    更新日期：2016-10-01 00:00:00

  • Effect of immobilization on the activity of rat hepatic microsomal cytochrome P450 enzymes.

    abstract：:Cytochrome P450 enzyme preparations were obtained from phenobarbital-treated rats. On immobilization in Romicon PM 10 polysulfone anisotropic hollow fibers, 20-25% of the original activity remained. The immobilization procedure did not significantly alter the Km of the preparations when 7-ethoxycoumarin was the substr...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/0141-0229(93)90031-v

    authors： Fernandez-Salguero P,Gutierrez-Merino C,Bunch AW

    更新日期：1993-02-01 00:00:00

  • Discovery and characterization of new O-methyltransferase from the genome of the lignin-degrading fungus Phanerochaete chrysosporium for enhanced lignin degradation.

    abstract：:Using bioinformatic homology search tools, this study utilized sequence phylogeny, gene organization and conserved motifs to identify members of the family of O-methyltransferases from lignin-degrading fungus Phanerochaete chrysosporium. The heterologous expression and characterization of O-methyltransferases from P. ...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2015.08.016

    authors： Thanh Mai Pham L,Kim YH

    更新日期：2016-01-01 00:00:00

  • Alginate as immobilization matrix and stabilizing agent in a two-phase liquid system: application in lipase-catalysed reactions.

    abstract：:Alginate was evaluated as an immobilization matrix for enzyme-catalyzed reactions in organic solvents. In contrast to most hydrogels, calcium alginate was found to be stable in a range of organic solvents and to retain the enzyme inside the gel matrix. In hydrophobic solvents, the alginate gel (greater than 95% water)...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/0141-0229(92)90024-i

    authors： Hertzberg S,Kvittingen L,Anthonsen T,Skjåk-Braek G

    更新日期：1992-01-01 00:00:00

  • Chemical modification of lysine residues in Bacillus alpha-amylases: effect on activity and stability.

    abstract：:Chemical modification of lysine residues in two bacterial alpha-amylases, a mesophilic enzyme from Bacillus amyloliquefaciens (BAA) and a thermophilic enzyme from Bacillus licheniformis (BLA) was carried out using citraconic anhydride. 13 +/- 1 residues in BAA and 10 +/- 1 residues in BLA were found modified under def...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/s0141-0229(01)00296-4

    authors： Khajeh K,Naderi-Manesh H,Ranjbar B,Moosavi-Movahedi A,Nemat-Gorgani M

    更新日期：2001-04-05 00:00:00

  • Intracellular synthesis of gold nanoparticles with antioxidant activity by probiotic Lactobacillus kimchicus DCY51T isolated from Korean kimchi.

    abstract：:A straightforward synthesis of gold nanoparticles (AuNps) is achieved by novel probiotic Lactobacillus kimchicus DCY51T isolated from Korean kimchi via an intracellular membrane-bound mechanism. The bioreduction of HAuCl4 into AuNps was verified by ultraviolet-visible spectrophotometry at ∼540nm. AuNps were spherical ...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2016.08.018

    authors： Markus J,Mathiyalagan R,Kim YJ,Abbai R,Singh P,Ahn S,Perez ZEJ,Hurh J,Yang DC

    更新日期：2016-12-01 00:00:00

  • Conversion of cysteine to 3-mercaptopyruvic acid by bacterial aminotransferases.

    abstract：:3-Mercaptopyruvate (3MPy), a structural analog of 3-mercaptopropionic acid, is a precursor compound for biosynthesis of polythioesters in bacteria. The cost-effectiveness and sustainability of the whole process could be greatly improved by using the cysteine degradation pathway for an intracellular supply of 3MPy. Tra...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2017.01.004

    authors： Andreeßen C,Gerlt V,Steinbüchel A

    更新日期：2017-04-01 00:00:00

  • Effect of protein load on stability of immobilized enzymes.

    abstract：:Different lipases have been immobilized on octyl agarose beads at 1mg/g and at maximum loading, via physical interfacial activation versus the octyl layer on the support. The stability of the preparations was analyzed. Most biocatalysts had the expected result: the apparent stability increased using the highly loaded ...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2016.12.002

    authors： Fernandez-Lopez L,Pedrero SG,Lopez-Carrobles N,Gorines BC,Virgen-Ortíz JJ,Fernandez-Lafuente R

    更新日期：2017-03-01 00:00:00

  • Purification and characterization of an extracellular alpha-amylase produced by Lactobacillus manihotivorans LMG 18010(T), an amylolytic lactic acid bacterium.

    abstract：:This work presents the purification and characterization of an extracellular alpha-amylase (1,4-alpha-D-glucan glucanohydrolase, EC 3.2.1.1) produced by a new lactic acid bacterium: Lactobacillus manihotivorans able to produce L(+) lactic acid from starch. The molecular weight was found to be 135 kDa. The temperature ...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/s0141-0229(00)00230-1

    authors： Aguilar G,Morlon-Guyot J,Trejo-Aguilar B,Guyot JP

    更新日期：2000-09-01 00:00:00

  • Purification, characterisation and expression in Saccharomyces cerevisiae of LipG7 an enantioselective, cold-adapted lipase from the Antarctic filamentous fungus Geomyces sp. P7 with unusual thermostability characteristics.

    abstract：:A lipase, LipG7, has been purified from the Antarctic filamentous fungus Geomyces sp. P7 which was found to be cold-adapted and able to retain/regain its activity after heat denaturation. The LipG7 exhibits 100% residual activity following 1h incubation at 100°C whilst simultaneously showing kinetic adaptations to col...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2013.03.021

    authors： Florczak T,Daroch M,Wilkinson MC,Białkowska A,Bates AD,Turkiewicz M,Iwanejko LA

    更新日期：2013-06-10 00:00:00

  • Genome mining and motif truncation of glycoside hydrolase family 5 endo-β-1,4-mannanase encoded by Aspergillus oryzae RIB40 for potential konjac flour hydrolysis or feed additive.

    abstract：:Two novel glycosyl hydrolase family 5 (GH5) β-mannanases (AoMan5A and AoMan5B) were identified from Aspergillus oryzae RIB40 by genome mining. The AoMan5A contains a predicted family 1 carbohydrate binding module (CBM-1), located at its N-terminal. The AoMan5A, AoMan5B and truncated mutant AoMan5AΔCL (truncating the N...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2016.08.003

    authors： Tang CD,Shi HL,Tang QH,Zhou JS,Yao LG,Jiao ZJ,Kan YC

    更新日期：2016-11-01 00:00:00

  • Kinetic resolution of sec-alcohols catalysed by Candida antarctica lipase B displaying Pichia pastoris whole-cell biocatalyst.

    abstract：:Kinetic resolution of sec-alcohols is a green process with biocatalyst. Candida antarctica lipase B (CALB) displayed on Pichia pastoris cell-surface (Pp-CALB) was characterized in kinetic resolution of sec-alcohols with different structures. The reaction parameters including acyl donors, molar ratio of substrates, sol...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2017.11.005

    authors： Zhang K,Pan Z,Diao Z,Liang S,Han S,Zheng S,Lin Y

    更新日期：2018-03-01 00:00:00

  • Increasing the refolding efficiency in vitro by site-directed mutagenesis of Cys383 in rat procarboxypeptidase B.

    abstract：:This study examines a novel method to reduce the probability of disulfide mismatches during the refolding process by the replacement of cysteines within a protein. Specifically, Cys383 of recombinant rat procarboxypeptidase B was replaced by other amino acids to increase the refolding efficiency in vitro. Mutants C383...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2011.04.018

    authors： Li S,Zhang L,Wu Q,Xin A,Zhao J,Fan L

    更新日期：2011-07-10 00:00:00

  • An efficient in vitro refolding of recombinant bacterial laccase in Escherichia coli.

    abstract：:Laccases (benzenediol oxygen oxidoreductases, EC 1.10.3.2) are important multicopper enzymes that are used in many biotechnological processes. A recombinant form of laccase from Bacillus sp. HR03 was overexpressed in Escherichia coli BL-21(DE3). Inclusion body (IB) formation happens quite often during recombinant prot...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2013.03.006

    authors： Mollania N,Khajeh K,Ranjbar B,Rashno F,Akbari N,Fathi-Roudsari M

    更新日期：2013-05-10 00:00:00

  • A single and two step isomerization process for d-tagatose and l-ribose bioproduction using l-arabinose isomerase and d-lyxose isomerase.

    abstract：:l-ribose and d-tagatose are biochemically synthesized using sugar isomerases. The l-arabinose isomerase gene from Shigella flexneri (Sf-AI) was cloned and expressed in Escherichia coli BL-21. Sf-AI was applied for the bioproduction of d-tagatose from d-galactose. l-ribose synthesis was performed by two step isomerizat...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2016.11.001

    authors： Patel MJ,Akhani RC,Patel AT,Dedania SR,Patel DH

    更新日期：2017-02-01 00:00:00

  • Catalytic synthesis of 2,5-bis(hydroxymethyl)furan from 5-hydroxymethylfurfual by recombinant Saccharomyces cerevisiae.

    abstract：:2,5-Bis(hydroxymethyl)furan (BHMF) is a versatile building block in the synthesis of polymers, fuels, and macrocycle polyethers. In this work, alcohol dehydrogenases (ADHs) were identified from Meyerozyma guilliermondii SC1103 and were heterologously expressed in Saccharomyces cerevisiae for the synthesis of BHMF from...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2019.109491

    authors： Xia ZH,Zong MH,Li N

    更新日期：2020-03-01 00:00:00

  • RNA interference with carbon catabolite repression in Trichoderma koningii for enhancing cellulase production.

    abstract：:The cellulase and xylanase genes of filamentous Trichoderma fungi exist under carbon catabolite repression mediated by the regulator carbon catabolite repressor (CREI). Our objective was to find the role of CREI in a cellulase-hyperproducing mutant of Trichoderma koningii, and address whether enzyme production can be ...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2013.04.007

    authors： Wang S,Liu G,Yu J,Tian S,Huang B,Xing M

    更新日期：2013-07-10 00:00:00

  • Secretory overexpression of the endoglucanase by Saccharomyces cerevisiae via CRISPR-δ-integration and multiple promoter shuffling.

    abstract：:Various recombinant proteins can be produced by the yeast Saccharomyces cerevisiae cell factories; therefore, efficient recombinant protein production techniques are desirable. In this study, to establish an efficient recombinant protein production technique in S. cerevisiae, the secretory production of recombinant pr...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2018.10.014

    authors： Sasaki Y,Mitsui R,Yamada R,Ogino H

    更新日期：2019-02-01 00:00:00

  • Simultaneous production of cellulase and reducing sugar through modification of compositional and structural characteristic of sugarcane bagasse.

    abstract：:This study examined the potential of untreated and alkali-pretreated sugarcane bagasse (SCB) in cellulase, reducing sugar (RS) and fungal biomass production via solid state fermentation (SSF) using Pycnoporus sanguineus. The impact of the composition, structure and cellulase adsorption ability of SCB on the production...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2013.05.005

    authors： Yoon LW,Ngoh GC,Chua AS

    更新日期：2013-09-10 00:00:00

  • Biochemical stability and molecular dynamic characterization of Aspergillus fumigatus cystathionine γ-lyase in response to various reaction effectors.

    abstract：:Cystathionine γ-lyase (CGL) is a key enzyme in the methionine-cysteine cycle in all living organisms forming cysteine, α-ketobutyrate and ammonia via homocysteine and cystathionine intermediates. Although, human and plant CGLs have been extensively studied at the molecular and mechanistic levels, there has been little...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2015.08.004

    authors： El-Sayed AS,Abdel-Azeim S,Ibrahim HM,Yassin MA,Abdel-Ghany SE,Esener S,Ali GS

    更新日期：2015-12-01 00:00:00

  • Mechanism of papain-catalyzed synthesis of oligo-tyrosine peptides.

    abstract：:Di-, tri-, and tetra-tyrosine peptides with angiotensin I-converting enzyme inhibitory activity were synthesized by papain-catalyzed polymerization of L-tyrosine ethyl ester in aqueous media at 30 °C. Varying the reaction pH from 6.0 to 7.5 and the initial concentration of the ester substrate from 25 to 100 mM, the hi...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2015.03.007

    authors： Mitsuhashi J,Nakayama T,Narai-Kanayama A

    更新日期：2015-07-01 00:00:00

  • Reaction strategies for the enzymatic synthesis of neopentyl glycol diheptanoate.

    abstract：:This work describes for the first time the green synthesis of neopentyl glycol diheptanoate in a solvent-free medium via an enzymatic pathway. The process has been carried out in an open-air reactor in order to ease water removal through evaporation and shift the chemical equilibrium towards product formation. The inh...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2019.109400

    authors： Serrano-Arnaldos M,García-Martínez JJ,Ortega-Requena S,Bastida J,Máximo F,Montiel MC

    更新日期：2020-01-01 00:00:00