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Cellulose oxidation and bleaching processes based on recombinant Myriococcum thermophilum cellobiose dehydrogenase.

Abstract：

:Myriococcum thermophilum cellobiose dehydrogenase (MtCDH) was expressed in Pichia pastoris using the pPICZαA expression vector under the control of methanol inducible AOX promoter. The purified recombinant MtCDH with a specific activity of 3.1 Umg(-1) was characterized to obtain kinetic constants for various carbohydrate substrates. Additionally, the C1 oxidation of the reducing ends of cellobiose, cellotetraose and maltotriose by MtCDH was verified by HPLC-MS. MtCDH was employed to oxidize several different cellulose-based materials by production of hydrogen peroxide. Based on the obtained results a one-pot enzymatic scouring/bleaching process for cotton fabrics was developed using pectinases as scouring agent and MtCDH to produce H(2)O(2) for bleaching. An average increase in whiteness (Berger) ΔE of 26 and an average 95% increase in wettability were observed in all MtCDH treated fabrics. In addition, MtCDH oxidized typical colored cotton flavonoids (morin, rutin, isoquercitrin).

journal_name

Enzyme Microb Technol

journal_title

Enzyme and microbial technology

authors

Flitsch A,Prasetyo EN,Sygmund C,Ludwig R,Nyanhongo GS,Guebitz GM

doi

10.1016/j.enzmictec.2012.10.007

subject

Has Abstract

pub_date

2013-01-10 00:00:00

pages

60-7

issue

1

eissn

0141-0229

issn

1879-0909

pii

S0141-0229(12)00175-5

journal_volume

52

pub_type

杂志文章

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