Stability of a recombinant shuttle plasmid in Bacillus subtilis and Escherichia coli.

abstract：:The methyl ester of 2-benzoxazolon-3-yl-acetic acid was used as an acyl donor in the penicillin amidase-catalysed transfer reaction to 7-aminodesacetoxycephalosporanic acid. The synthesis of 7-(2-benzoxazolon-3-yl-acetamido)-desacetoxycephalosporanic acid was carried out as a kinetically controlled reaction. A charact...

journal_title：Enzyme and microbial technology

authors： Stambolieva N,Mincheva Z,Galunsky B,Kalcheva V

更新日期：1992-06-01 00:00:00

abstract：:The gene encoding a cold-adapted phospholipase A(1) (PLA(1)) from a psychrotrophic, glacier soil bacterium Serratia sp. xjF1 was cloned by two-step PCR (general PCR and TAIL-PCR). The full-length fragment comprised two open reading frames plA and plS. The gene product of plA encoding 320 amino acids with a molecular w...

journal_title：Enzyme and microbial technology

authors： Fu J,Huang H,Meng K,Yuan T,Yao B,Shi Y,Ouyang P

更新日期：2008-01-01 00:00:00

abstract：:A solvent-mediated method (SMM) was used to prepare supersaturated sugar solutions in hydrophobic and mixture of hydrophilic/hydrophobic ionic liquids (ILs), namely, [Bmim][Tf2N] and [Bmim][TfO]/[Bmim][Tf2N], respectively. In this method, sugars were first solubilized in a mixture of organic solvent and water (i.e. me...

journal_title：Enzyme and microbial technology

authors： Shin DW,Mai NL,Bae SW,Koo YM

更新日期：2019-07-01 00:00:00

abstract：:Growth conditions for mass production of luciferase-bacterial magnetic particles (BMPs) by a recombinant Magnetospirillum magneticum AMB-1 were investigated in a pH-regulated fed-batch culture system. Enrichment of growth medium with L-cysteine, yeast extract and polypeptone enhanced both bacterial growth and BMP prod...

journal_title：Enzyme and microbial technology

authors： Yang C,Takeyama H,Tanaka T,Matsunaga T

更新日期：2001-07-05 00:00:00

abstract：:The cellulase and xylanase genes of filamentous Trichoderma fungi exist under carbon catabolite repression mediated by the regulator carbon catabolite repressor (CREI). Our objective was to find the role of CREI in a cellulase-hyperproducing mutant of Trichoderma koningii, and address whether enzyme production can be ...

journal_title：Enzyme and microbial technology

authors： Wang S,Liu G,Yu J,Tian S,Huang B,Xing M

更新日期：2013-07-10 00:00:00

abstract：:Phospholipase C (PLC) activity secreted by bacteria as a virulence factor is commonly detected by use of the artificial substrate p-nitrophenylphosphorylcholine (p-NPPC). We examined several commercially available enzymes (phosphodiesterases, phosphomonoesterases, phospholipase A, lipase, protease) for their hydrolyti...

journal_title：Enzyme and microbial technology

authors： Fliegera A,Gong S,Faigle M,Neumeister B

更新日期：2000-03-01 00:00:00

abstract：:Oxygen uptake rate and oxygen mass transfer rate have been studied during xanthan gum production process in stirred tank bioreactor. Empirical equations for the oxygen mass transfer coefficient have been obtained taking into account several variables such as air flow rate, stirrer speed and apparent viscosity. Oxygen ...

journal_title：Enzyme and microbial technology

authors： García-Ochoa F,Castro EG,Santos VE

更新日期：2000-11-15 00:00:00

abstract：:In the present investigation, the bacterium Escherichia coli (VM1) was isolated from a marine sediments of Ennore coastal water along southeast coast of India was exploited for its capability of Ag NPs synthesis. The derived nanoparticles were characterized employing ultraviolet-visible (UV-vis) spectroscopy, Fourier ...

journal_title：Enzyme and microbial technology

authors： Maharani V,Sundaramanickam A,Balasubramanian T

更新日期：2016-12-01 00:00:00

abstract：:The D-amino acid oxidase cDNA gene (daao) of Trigonopsis variabilis was prepared by reverse transcriptase-polymerase chain reaction (PCR) and cloned into Escherichia coli expression vector, pTrc99A, under the control of tac promoter. Expression of daao gene significantly affected the growth and morphology of E. coli. ...

journal_title：Enzyme and microbial technology

authors： Lin L,Chien HR,Wang W,Hwang T,Fu H,Hsu W

更新日期：2000-10-01 00:00:00

abstract：:This study examines a novel method to reduce the probability of disulfide mismatches during the refolding process by the replacement of cysteines within a protein. Specifically, Cys383 of recombinant rat procarboxypeptidase B was replaced by other amino acids to increase the refolding efficiency in vitro. Mutants C383...

journal_title：Enzyme and microbial technology

authors： Li S,Zhang L,Wu Q,Xin A,Zhao J,Fan L

更新日期：2011-07-10 00:00:00

abstract：:The heat and mass transfer have been proved to be the important factors in air pressure pulsation for cellulase production. However, as process of enzyme secretion, the cellulase formation has not been studied in the view of microorganism metabolism and metabolic key enzyme activity under air pressure pulsation condit...

journal_title：Enzyme and microbial technology

authors： Chen H,Shao M,Li H

更新日期：2014-03-05 00:00:00

abstract：:Foaming, a proficient method for the recovery of surface active solutes from dilute solutions, was successfully applied for the concentration of the lipopeptide biosurfactant Surfactin from B. subtilis ATCC 21332 cell culture broths. Foaming was only partially successful in concentrating Surfactin when applied as a se...

journal_title：Enzyme and microbial technology

authors： Davis DA,Lynch HC,Varley J

更新日期：2001-03-08 00:00:00

abstract：:Before applying nanotechnologies in biomedical and environmental areas it is advised to study interactions of nanoparticles and other nanomaterials with biomacromolecule present in living system. Moreover there is scarcity of reports on interactions between nanoparticles and biomaterials. In present report a rapid, ec...

journal_title：Enzyme and microbial technology

authors： Patil CD,Borase HP,Suryawanshi RK,Patil SV

更新日期：2016-10-01 00:00:00

abstract：:Supercritical fluids offer environmental advantages over chemical solvents, while providing enhanced separation and chemical selectivity. The use of supercritical fluids for the recovery of products from biomass and the transformation of selected molecules (to add value) was studied. Free fatty acids were bio-catalyti...

journal_title：Enzyme and microbial technology

authors： Baig MN,Santos RC,Zetzl C,King J,Pioch D,Bowra S

更新日期：2011-09-10 00:00:00

abstract：:Flocculent yeast Saccharomyces cerevisiae YF234 (MATa ura3-52 trp1Delta2 his ade 2-1 can1-100 sta1 FLO8) cells overexpressing glyoxalase I and having strong flocculation ability were permeabilized with isopropyl alcohol and ethanol under various conditions. The treatment with 40% isopropyl alcohol significantly improv...

journal_title：Enzyme and microbial technology

authors： Kondo A,Liu Y,Furuta M,Fujita Y,Matsumoto T,Fukuda H

更新日期：2000-12-01 00:00:00

abstract：:We report a new colorimetric assay to quantify endo-polygalacturonase activity, which hydrolyzes polygalacturonic acid to produce smaller chains of galacturonate. Some of the reported polygalacturonase assays measure the activity by detecting the appearance of reducing ends such as the Somogyi-Nelson method. As a resu...

journal_title：Enzyme and microbial technology

authors： Torres S,Sayago JE,Ordoñez RM,Isla MI

更新日期：2011-02-08 00:00:00

abstract：:Alginate was evaluated as an immobilization matrix for enzyme-catalyzed reactions in organic solvents. In contrast to most hydrogels, calcium alginate was found to be stable in a range of organic solvents and to retain the enzyme inside the gel matrix. In hydrophobic solvents, the alginate gel (greater than 95% water)...

journal_title：Enzyme and microbial technology

authors： Hertzberg S,Kvittingen L,Anthonsen T,Skjåk-Braek G

更新日期：1992-01-01 00:00:00

abstract：:During toluene hydroxylation, catalyzed by Pseudomonas putida UV4 one molecule of oxygen is added to the aromatic ring to produce the dihydroxylated (non-aromatic) ring structure, toluene cis-glycol. Toluene, which is toxic to the cells at aqueous phase concentration above ( approximately 2.4 mmol), is fed to the reac...

journal_title：Enzyme and microbial technology

authors： Carragher JM,McClean WS,Woodley JM,Hack CJ

更新日期：2001-02-01 00:00:00

abstract：:Kumamolisin from Alicyclobacillus sendaiensis strain NTAP-1 is a serine protease with collagenase activity. After molecular engineering, a kumamolisin mutant, named Kuma030, was obtained with high proteolytic activity against gluten, which might cause celiac disease. Kuma030 exhibited its potential application in indu...

journal_title：Enzyme and microbial technology

authors： Liu H,Fan X,Song H,Hu X,Zhang G,Yu C,Yi L

更新日期：2019-10-01 00:00:00

abstract：:Biocatalytic peptide synthesis will benefit from enzymes that are active at low water levels in organic solvent compositions that allow good substrate and product solubility. To explore the use of proteases from thermophiles for peptide synthesis under such conditions, putative protease genes of the subtilase class we...

journal_title：Enzyme and microbial technology

authors： Toplak A,Nuijens T,Quaedflieg PJ,Wu B,Janssen DB

更新日期：2015-06-01 00:00:00

abstract：:In the previous study, glutathione production was elevated by adding precursor amino acids and adenosine triphosphate (ATP) in high cell density cultivation of Candida utilis. Furthermore, in the present research, glutathione production was further improved by optimizing sodium dedecyl sulfate (SDS) addition coupled w...

journal_title：Enzyme and microbial technology

authors： Liang G,Mo Y,Du G

更新日期：2010-05-05 00:00:00

abstract：:The kinetic and thermodynamic parameters of wheat β-amylase (WBA) were characterized and various additives were evaluated for enhancing its activity and thermostability. WBA activity was examined by neocuproine method using soluble starch as substrate. The Michaelis constant (K(m)) and molecular activity (k(cat)) were...

journal_title：Enzyme and microbial technology

authors： Daba T,Kojima K,Inouye K

更新日期：2012-10-10 00:00:00

abstract：:The primary aim of this study was to decipher the catalytic functions of the NHase with wide substrate spectra from Rhodococcus ruber CGMCC3090 by computer modeling and substrate docking. 3D structure model of the enzyme was built by computer modeling to obtain the optimal structure. The larger binding site cavity (55...

journal_title：Enzyme and microbial technology

authors： Wang S,Dai Y,Wang J,Shen Y,Zhai Y,Zheng H,Wang M

更新日期：2013-02-05 00:00:00

abstract：:The mass distribution regularity in substrate of solid-state fermentation (SSF) has rarely been reported due to the heterogeneity of solid medium and the lack of suitable instrument and method, which limited the comprehensive analysis and enhancement of the SSF performance. In this work, the distributions of water, bi...

journal_title：Enzyme and microbial technology

authors： Chen HZ,Zhao ZM,Li HQ

更新日期：2014-05-10 00:00:00

abstract：:We generated a bifunctional enzyme chimera containing the xylanase and lichenase coupled with SpyTag between them. Meanwhile, we generated another chimera containing SpyCatcher and elastin-like polypeptides (ELPs). As ELPs could bond to the xylanase-lichenase chimera through SpyTag/SpyCatcher spontaneous reaction in m...

journal_title：Enzyme and microbial technology

authors： Lin Y,Jin W,Wang J,Cai Z,Wu S,Zhang G

更新日期：2018-08-01 00:00:00

abstract：:Zymomonas mobilis genes encoding INVA and INVB were expressed in Pichia pastoris, under the control of the strong AOX1 promoter, and the recombinant enzymes were named INVAAOX1 and INVBAOX1. The expression levels of INVAAOX1 (1660 U/mg) and INVBAOX1 (1993 U/mg) in P. pastoris were 9- and 7-fold higher than those obser...

journal_title：Enzyme and microbial technology

authors： Pérez de los Santos AI,Cayetano-Cruz M,Gutiérrez-Antón M,Santiago-Hernández A,Plascencia-Espinosa M,Farrés A,Hidalgo-Lara ME

更新日期：2016-02-01 00:00:00

abstract：:beta-amylases are used in production of maltose syrup. It is shown that sweet potato beta-amylase can be purified by affinity precipitation with alginate with 80% activity yield and 44 fold purification. SDS-PAGE of the purified protein showed a single band and a subunit weight of 50 kDa. Preliminary data with soybean...

journal_title：Enzyme and microbial technology

authors： Teotia S,Khare SK,Gupta MN

更新日期：2001-06-07 00:00:00

abstract：:A three-phase fluidized bed reactor (TPFBR) was designed to evaluate the potential of CoQ(10) production by gel-entrapped Sphingomonas sp. ZUTE03 via a conversion-extract coupled process. In the reactor, the CoQ(10) yield reached 46.99 mg/L after 8 h of conversion; a high-level yield of about 45 mg/L was maintained ev...

journal_title：Enzyme and microbial technology

authors： Qiu L,Ding H,Wang W,Kong Z,Li X,Shi Y,Zhong W

更新日期：2012-02-10 00:00:00

abstract：:This study reports a general strategy for the encapsulation of various enzymes in amphiphilic hollow carbonaceous microspheres (CMs). We found that enzymes could be spontaneously encapsulated in the interior cavity of the CMs via hydrophobic interactions. Due to strong hydrophobic interactions and robust confinement, ...

journal_title：Enzyme and microbial technology

authors： Liu L,Sun Y,He L,Jiang L,Yang S

更新日期：2015-11-01 00:00:00

abstract：:Resveratrol is a well-known polyphenol present in red wine and exerts antioxidative and anti-carcinogenic effects on the human body. To produce resveratrol in a food-grade yeast, the 4-coumarate:coenzyme A ligase gene (4CL1) from Arabidopsis thaliana and stilbene synthase gene (STS) from Arachis hypogaea were cloned a...

journal_title：Enzyme and microbial technology

authors： Shin SY,Han NS,Park YC,Kim MD,Seo JH

更新日期：2011-01-05 00:00:00