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Preparation of high activity whole cell biocatalyst by permeabilization of recombinant flocculent yeast with alcohol.

Abstract：

:Flocculent yeast Saccharomyces cerevisiae YF234 (MATa ura3-52 trp1Delta2 his ade 2-1 can1-100 sta1 FLO8) cells overexpressing glyoxalase I and having strong flocculation ability were permeabilized with isopropyl alcohol and ethanol under various conditions. The treatment with 40% isopropyl alcohol significantly improves the initial reaction rates of recombinant flocculent yeast cells. Moreover, the reactivity of permeabilized flocculent yeast cells was similar to that of dispersed cells with EDTA. On the other hand, the flocculation ability of yeast cells was not affected by the treatment with alcohol solutions of various concentrations and treatment time length. Therefore, the recombinant flocculent yeast cells permeabilized with alcohol are very effective whole cell biocatalysts.

journal_name

Enzyme Microb Technol

journal_title

Enzyme and microbial technology

authors

Kondo A,Liu Y,Furuta M,Fujita Y,Matsumoto T,Fukuda H

doi

10.1016/s0141-0229(00)00304-5

keywords:

subject

Has Abstract

pub_date

2000-12-01 00:00:00

pages

806-811

issue

10

eissn

0141-0229

issn

1879-0909

pii

S0141022900003045

journal_volume

27

pub_type

杂志文章

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