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The potential of positively-charged cellulose sponge for malolactic fermentation of wine, using Oenococcus oeni.

Abstract：

:Malolactic fermentation (MLF) is a secondary bioconversion developed in some wines involving malic acid decarboxylation. The induction of MLF in wine by cultures of free and immobilized Oenococcus oeni cells was investigated. This work reports on the effect of surface charges in the immobilization material, a recently described fibrous sponge, as well as the pH and the composition of the media where cells are suspended. A chemical treatment provided positive charge to the sponges (DE or DEAE) and gave the highest cell loadings and subsequent resistance to removal. Preculture media to grow the malolactic bacteria before the immobilization procedure were also evaluated. We have established favorable conditions for growth (Medium of Preculture), suspension solution (Tartrate-Phosphate Buffer), suspension pH (3.5-5.5) and immobilization matrix (DE or DEAE cellulose sponge) to induce MLF in red wine. The use of a semi-continuous system permitted a high-efficiency malic acid conversion by 2 x 10(9) cfu sponge(-)(1) in at least four subsequent batch fermentations.

journal_name

Enzyme Microb Technol

journal_title

Enzyme and microbial technology

authors

Maicas S,Pardo I,Ferrer S

doi

10.1016/s0141-0229(00)00339-2

keywords:

subject

Has Abstract

pub_date

2001-03-08 00:00:00

pages

415-419

issue

4-5

eissn

0141-0229

issn

1879-0909

pii

S0141022900003392

journal_volume

28

pub_type

杂志文章

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