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The kinetics of affinity-mediated cell-surface attachment.

Abstract：

:Data and a semi-empirical model are presented that describe the affinity interaction of yeast cells with a Concanavalin A derivatised surface. The model uses 3 parameters to describe the time course of cell attachment from a flowing suspension of yeast cells, over a range of flow rates, and gives an effective global fit to the data obtained. Further modifications allow the effects of a soluble competitor (glucose) on binding to be quantified in terms of a saturation effect, and an effective global fit is obtained. A comparison was made between the relationship between steady-state attached fraction and applied shear with similar data reported earlier (Ming, F. et al, 1998) for the detachment of pre-adsorbed cells. This shows that there is an order of magnitude difference between the forces required to effect complete detachment in the two systems, and that the nature of the relationship between shear and attached fraction is profoundly different. The magnitude of this time-dependent stabilization might be explained in terms of a progressive reorientation of cell relative to the surface such that the number of bonds is maximized.

journal_name

Enzyme Microb Technol

journal_title

Enzyme and microbial technology

authors

Ming F,Eisenthal R,Whish WJ,Hubble J

doi

10.1016/s0141-0229(99)00161-1

keywords:

subject

Has Abstract

pub_date

2000-02-01 00:00:00

pages

216-221

issue

2-4

eissn

0141-0229

issn

1879-0909

pii

S0141022999001611

journal_volume

26

pub_type

杂志文章

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