CAH1 and CAH2 as key enzymes required for high bicarbonate tolerance of a novel microalga Dunaliella salina HTBS.


:Outdoor microalgal cultivation with high concentration bicarbonate has been considered as a strategy for reducing contamination and improving carbon supply efficiency. The mechanism responsible for algae's strong tolerance to high bicarbonate however, remains not clear. In this study, we isolated and characterized a strain and revealed its high bicarbonate tolerant mechanism by analyzing carbonic anhydrase (CA). The strain was identified as Dunaliella salina HTBS with broad temperature adaptability (7-30°C). The strain grew well under 30% CO2 or 70gL(-1) NaHCO3. In comparison, two periplasm CAs (CAH1 and CAH2) were detected with immunoblotting analysis in HTBS but not in a non-HCO3(-)-tolerant strain. The finding was also verified by an enzyme inhibition assay in which only HTBS showed significant inhibition by extracellular CA inhibitor. Thus, we inferred that the extracellular CAH1 and CAH2 played a multifunctional role in the toleration of high bicarbonate by HTBS.


Enzyme Microb Technol


Hou Y,Liu Z,Zhao Y,Chen S,Zheng Y,Chen F




Has Abstract


2016-06-01 00:00:00












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    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Krzek M,van Beek HL,Permentier HP,Bischoff R,Fraaije MW

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    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


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    authors: Ettalibi M,Baratti JC

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    authors: Jung IP,Cho JH,Koo BS,Yoon MY

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    journal_title:Enzyme and microbial technology

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    journal_title:Enzyme and microbial technology

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    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


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    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Florczak T,Daroch M,Wilkinson MC,Białkowska A,Bates AD,Turkiewicz M,Iwanejko LA

    更新日期:2013-06-10 00:00:00

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    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Chen HZ,Zhao ZM,Li HQ

    更新日期:2014-05-10 00:00:00

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    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: McDermott JF,Lethbridge G,Bushell ME

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    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


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    更新日期:2016-12-01 00:00:00

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    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Fernandez-Lopez L,Pedrero SG,Lopez-Carrobles N,Gorines BC,Virgen-Ortíz JJ,Fernandez-Lafuente R

    更新日期:2017-03-01 00:00:00