Purification, characterisation and expression in Saccharomyces cerevisiae of LipG7 an enantioselective, cold-adapted lipase from the Antarctic filamentous fungus Geomyces sp. P7 with unusual thermostability characteristics.

abstract：:Foaming, a proficient method for the recovery of surface active solutes from dilute solutions, was successfully applied for the concentration of the lipopeptide biosurfactant Surfactin from B. subtilis ATCC 21332 cell culture broths. Foaming was only partially successful in concentrating Surfactin when applied as a se...

journal_title：Enzyme and microbial technology

authors： Davis DA,Lynch HC,Varley J

更新日期：2001-03-08 00:00:00

abstract：:A chemiluminescence fiber optic system coupled to flow injection analysis (FIA) and ion exchange chromatography has been developed for determining glucose in blood and urine. Immobilized glucose oxidase acted on beta-D-glucose to produce hydrogen peroxide, which was then reacted with luminol in the presence of ferricy...

journal_title：Enzyme and microbial technology

authors： Cattaneo MV,Luong JH

更新日期：1993-05-01 00:00:00

abstract：:Mycobacterium tuberculosis acetohydroxyacid synthase (M. tuberculosis AHAS) has been proposed to bean essential target for novel herbicide- and chemical-based antibacterial agents. Therefore, here we investigated the roles of multiple conserved herbicide-binding site residues (R318, A146, Q148, M512, and V513) in M. t...

journal_title：Enzyme and microbial technology

authors： Jung IP,Cho JH,Koo BS,Yoon MY

更新日期：2015-10-01 00:00:00

abstract：:In this work, porous biochar derived from sugarcane bagasse was prepared and then coated with different amounts of chitosan (C@CS) for cellulase immobilization. Cellulase was covalently immobilized on the support by using glutaraldehyde as a linker. The chemical characteristics and morphology of the samples were deter...

journal_title：Enzyme and microbial technology

authors： Mo H,Qiu J,Yang C,Zang L,Sakai E,Chen J

更新日期：2020-08-01 00:00:00

abstract：:A generic approach for flavoenzyme immobilization was developed in which the flavin cofactor is used for anchoring enzymes onto the carrier. It exploits the tight binding of flavin cofactors to their target apo proteins. The method was tested for phenylacetone monooxygenase (PAMO) which is a well-studied and industria...

journal_title：Enzyme and microbial technology

authors： Krzek M,van Beek HL,Permentier HP,Bischoff R,Fraaije MW

更新日期：2016-01-01 00:00:00

abstract：:A new bi-modular, wide pH spectrum and highly active xylanase KRICT PX3 (JF320814) isolated from Paenibacillus terrae HPL-003 (KCTC11987BP) has been cloned and expressed in Escherichia coli. Purified recombinant xylanase KRICT PX-3 (1,620 bp, 540aa, NCBI accession number JF320814) showed highly active at 55°C in pH 4....

journal_title：Enzyme and microbial technology

authors： Song HY,Lim HK,Kim DR,Lee KI,Hwang IT

更新日期：2014-01-10 00:00:00

abstract：:This study examines a novel method to reduce the probability of disulfide mismatches during the refolding process by the replacement of cysteines within a protein. Specifically, Cys383 of recombinant rat procarboxypeptidase B was replaced by other amino acids to increase the refolding efficiency in vitro. Mutants C383...

journal_title：Enzyme and microbial technology

authors： Li S,Zhang L,Wu Q,Xin A,Zhao J,Fan L

更新日期：2011-07-10 00:00:00

abstract：:Thiol dioxygenases are non-heme mononuclear-iron proteins and belong to the cupin superfamily. In 2014, mercaptosuccinate dioxygenase (Msdo) of Variovorax paradoxus B4 was identified as another bacterial cysteine dioxygenase (Cdo) homolog catalyzing the conversion of mercaptosuccinate (MS) into succinate and sulfite. ...

journal_title：Enzyme and microbial technology

authors： Brandt U,Galant G,Meinert-Berning C,Steinbüchel A

更新日期：2019-01-01 00:00:00

abstract：:GH11 xylanase XynJ from Bacillus sp. strain 41M-1 has a β-jellyroll fold composed of eight β strands with a deep active-site cleft. We hypothesized that the thermostability of XynJ will increase if the flexibility of the β strands in the jellyroll structure is decreased without impairing activity. To verify this hypot...

journal_title：Enzyme and microbial technology

authors： Takita T,Nakatani K,Katano Y,Suzuki M,Kojima K,Saka N,Mikami B,Yatsunami R,Nakamura S,Yasukawa K

更新日期：2019-11-01 00:00:00

abstract：:Progesterone 5β-reductases (P5βRs) are involved in 5β-cardenolide formation by stereo-specific reduction of the △4,5 double bond of steroid precursors. In this study a steroid 5β-reductase was identified in Capsella rubella (CrSt5βR1) and its function in steroid 5β-reduction was validated experimentally. CrSt5βR1 is c...

journal_title：Enzyme and microbial technology

authors： Li Y,Pan H,Chang Y,Dong N,Zou L,Liang P,Tian W,Chang Z

更新日期：2020-03-01 00:00:00

abstract：:Advantages in maltose fermentation at extremely low temperatures (5-10°C) using an alcohol resistant and cryotolerant yeast strain (Saccharomyces cerevisiae AXAZ-1) immobilized on porous cellulosic material (or tubular cellulose, TC), produced by delignification of wood sawdust, are reported. Pure maltose and glucose ...

journal_title：Enzyme and microbial technology

authors： Ganatsios V,Koutinas AA,Bekatorou A,Kanellaki M,Nigam P

更新日期：2014-11-01 00:00:00

abstract：:Turanose is a natural isomer of sucrose. It is an emerging functional sweetener of the next generation. Turanose is catalytically synthesized from the sucrose biomass by employing amylosucrase enzyme. In this study, a novel gene encoding amylosucrase (Asmet) has been identified from the metagenome of a thermal aquatic...

journal_title：Enzyme and microbial technology

authors： Agarwal N,Narnoliya LK,Singh SP

更新日期：2019-12-01 00:00:00

abstract：:The substrate specificities of alpha-chymotrypsin and subtilisins for peptide synthesis in hydrophilic organic solvents were investigated. Chymotrypsin exhibited high specificity to aromatic amino acids as acyl donors, while subtilisin Carlsberg and subtilisin BPN' were specific to aromatic and neutral aliphatic amino...

journal_title：Enzyme and microbial technology

authors： Nagashima T,Watanabe A,Kise H

更新日期：1992-10-01 00:00:00

abstract：:Hydrolysis of lignocellulosic biomass depends on the concerted actions of cellulases and accessory proteins. In this work we examined the combined action of two auxiliary proteins from the brown rot fungus Gloeophyllum trabeum: a family AA9 lytic polysaccharide monooxygenase (GtLPMO) and a GH10 xylanase (GtXyn10A). Th...

journal_title：Enzyme and microbial technology

authors： Sanhueza C,Carvajal G,Soto-Aguilar J,Lienqueo ME,Salazar O

更新日期：2018-06-01 00:00:00

abstract：:A novel magnetic Fe3O4 nanoparticles (MNPs) coupled with agarose (AMNPs) was synthesized using co-precipitation via alkaline condition and span-80 surfactants in organic solvent. Iminodiacetate was first attached to the MNPs through epichlorohydrin agent and then chelated with metal ions. The morphology and chemical p...

journal_title：Enzyme and microbial technology

authors： Chen T,Yang W,Guo Y,Yuan R,Xu L,Yan Y

更新日期：2014-09-01 00:00:00

abstract：:Resveratrol is a well-known polyphenol present in red wine and exerts antioxidative and anti-carcinogenic effects on the human body. To produce resveratrol in a food-grade yeast, the 4-coumarate:coenzyme A ligase gene (4CL1) from Arabidopsis thaliana and stilbene synthase gene (STS) from Arachis hypogaea were cloned a...

journal_title：Enzyme and microbial technology

authors： Shin SY,Han NS,Park YC,Kim MD,Seo JH

更新日期：2011-01-05 00:00:00

abstract：:This study reports a general strategy for the encapsulation of various enzymes in amphiphilic hollow carbonaceous microspheres (CMs). We found that enzymes could be spontaneously encapsulated in the interior cavity of the CMs via hydrophobic interactions. Due to strong hydrophobic interactions and robust confinement, ...

journal_title：Enzyme and microbial technology

authors： Liu L,Sun Y,He L,Jiang L,Yang S

更新日期：2015-11-01 00:00:00

abstract：:Isolates from gardening waste compost and 38 culture collection microbes were grown on agar plates at pH 4.0 with the cutinase model substrate polycaprolactone as a carbon source. The strains showing polycaprolactone hydrolysis were cultivated in liquid at acidic pH and the cultivations were monitored by assaying the ...

journal_title：Enzyme and microbial technology

authors： Nyyssölä A,Pihlajaniemi V,Järvinen R,Mikander S,Kontkanen H,Kruus K,Kallio H,Buchert J

更新日期：2013-04-10 00:00:00

abstract：:This study examined the potential of untreated and alkali-pretreated sugarcane bagasse (SCB) in cellulase, reducing sugar (RS) and fungal biomass production via solid state fermentation (SSF) using Pycnoporus sanguineus. The impact of the composition, structure and cellulase adsorption ability of SCB on the production...

journal_title：Enzyme and microbial technology

authors： Yoon LW,Ngoh GC,Chua AS

更新日期：2013-09-10 00:00:00

abstract：:Amylosucrase (ASase) has great industrial potential owing to its multifunctional activities, including transglucosylation, polymerization, and isomerization. In the present study, the properties of Deinococcus geothermalis ASase (DGAS) expressed in Corynebacterium glutamicum (cDGAS) and purified via Ni-NTA affinity ch...

journal_title：Enzyme and microbial technology

authors： Chin YW,Jang SW,Shin HS,Kim TW,Kim SK,Park CS,Seo DH

更新日期：2020-04-01 00:00:00

abstract：:A novel endoglucanase active on 1,3-1,4-beta-D-glucans was purified to apparent homogeneity from submerged cultures of the moderately thermophilic aerobic fungus Talaromyces emersonii CBS 814.70. The enzyme is a single subunit glycoprotein with M(r) and pI values of 40.7 +/- 0.3 kDa and 4.4, respectively, and an estim...

journal_title：Enzyme and microbial technology

authors： Murray PG,Grassick A,Laffey CD,Cuffe MM,Higgins T,Savage AV,Planas A,Tuohy MG

更新日期：2001-07-05 00:00:00

abstract：:Cytochrome P450 enzyme preparations were obtained from phenobarbital-treated rats. On immobilization in Romicon PM 10 polysulfone anisotropic hollow fibers, 20-25% of the original activity remained. The immobilization procedure did not significantly alter the Km of the preparations when 7-ethoxycoumarin was the substr...

journal_title：Enzyme and microbial technology

authors： Fernandez-Salguero P,Gutierrez-Merino C,Bunch AW

更新日期：1993-02-01 00:00:00

abstract：:Effects of pulse and step changes in major metabolite concentrations such as glucose, glutamine, lactic acid, and ammonium on behavior of T-cell hybridomas in continuous culture were investigated in order to develop a strategy to maximize specific rate of lymphokine formation. Step increases in glucose, lactic acid, a...

journal_title：Enzyme and microbial technology

authors： Shaughnessy TS,Kargi F

更新日期：1990-09-01 00:00:00

abstract：:Malolactic fermentation (MLF) is a secondary bioconversion developed in some wines involving malic acid decarboxylation. The induction of MLF in wine by cultures of free and immobilized Oenococcus oeni cells was investigated. This work reports on the effect of surface charges in the immobilization material, a recently...

journal_title：Enzyme and microbial technology

authors： Maicas S,Pardo I,Ferrer S

更新日期：2001-03-08 00:00:00

abstract：:Bacterial chitinases recruited multiple accessory domains for the conversion of recalcitrant polysaccharides to simple soluble sugars/amino sugars. Here, we report detailed properties of a multi-domain GH18 chitinase from Enterobacter cloacae subsp. cloacae (EcChi2) that preferred β-chitin as substrate. EcChi2 exhibit...

journal_title：Enzyme and microbial technology

authors： Mallakuntla MK,Podile AR

更新日期：2021-02-01 00:00:00

abstract：:The gene encoding a cold-adapted phospholipase A(1) (PLA(1)) from a psychrotrophic, glacier soil bacterium Serratia sp. xjF1 was cloned by two-step PCR (general PCR and TAIL-PCR). The full-length fragment comprised two open reading frames plA and plS. The gene product of plA encoding 320 amino acids with a molecular w...

journal_title：Enzyme and microbial technology

authors： Fu J,Huang H,Meng K,Yuan T,Yao B,Shi Y,Ouyang P

更新日期：2008-01-01 00:00:00

abstract：:This study aimed to develop viable enzymes for bioconversion of resveratrol-glucoside into resveratrol. Out of 13 bacterial strains tested, Lactobacillus kimchi JB301 could completely convert polydatin into resveratrol. The purified enzyme had an optimum temperature of 30-40°C and optimum pH of pH 5.0 against polydati...

journal_title：Enzyme and microbial technology

authors： Ko JA,Park JY,Kwon HJ,Ryu YB,Jeong HJ,Park SJ,Kim CY,Oh HM,Park CS,Lim YH,Kim D,Rho MC,Lee WS,Kim YM

更新日期：2014-12-01 00:00:00

abstract：:We report a new colorimetric assay to quantify endo-polygalacturonase activity, which hydrolyzes polygalacturonic acid to produce smaller chains of galacturonate. Some of the reported polygalacturonase assays measure the activity by detecting the appearance of reducing ends such as the Somogyi-Nelson method. As a resu...

journal_title：Enzyme and microbial technology

authors： Torres S,Sayago JE,Ordoñez RM,Isla MI

更新日期：2011-02-08 00:00:00

abstract：:The kinetic and thermodynamic parameters of wheat β-amylase (WBA) were characterized and various additives were evaluated for enhancing its activity and thermostability. WBA activity was examined by neocuproine method using soluble starch as substrate. The Michaelis constant (K(m)) and molecular activity (k(cat)) were...

journal_title：Enzyme and microbial technology

authors： Daba T,Kojima K,Inouye K

更新日期：2012-10-10 00:00:00

abstract：:Addition of non-ionic surfactants (NIS) is known to accelerate enzymatic lignocellulose hydrolysis. The mechanism behind this accelerating effect is still not elucidated but has been hypothesized to originate from favorable NIS-lignin interactions which alleviate non-productive adsorption of cellulases to lignin. In t...

journal_title：Enzyme and microbial technology

authors： Olsen SN,Bohlin C,Murphy L,Borch K,McFarland KC,Sweeny MD,Westh P

更新日期：2011-09-10 00:00:00