当前位置： SCI文献检索 > ENZYME AND MICROBIAL TECHNOLOGY期刊下所有文献 > Purification and functional characterization of the first stilbene glucoside-specific β-glucosidase isolated from Lactobacillus kimchi.

Purification and functional characterization of the first stilbene glucoside-specific β-glucosidase isolated from Lactobacillus kimchi.

Abstract：

:This study aimed to develop viable enzymes for bioconversion of resveratrol-glucoside into resveratrol. Out of 13 bacterial strains tested, Lactobacillus kimchi JB301 could completely convert polydatin into resveratrol. The purified enzyme had an optimum temperature of 30-40°C and optimum pH of pH 5.0 against polydatin. This enzyme showed high substrate specificities towards different substrates in the following order: isorhaponticin>polydatin>mulberroside A>oxyresveratrol-3-O-glucoside. Additionally, it rarely hydrolyzed astringin and desoxyrhaponticin. Based on these catalytic specificities, we suggest this enzyme be named stilbene glucoside-specific β-glucosidase. Furthermore, polydatin extracts from Polygonum cuspidatum were successfully converted to resveratrol with a high yield (of over 99%). Stilbene glucoside-specific β-glucosidase is the first enzyme isolated from lactic acid bacteria capable of bio-converting various stilbene glucosides into stilbene.

journal_name

Enzyme Microb Technol

journal_title

Enzyme and microbial technology

authors

Ko JA,Park JY,Kwon HJ,Ryu YB,Jeong HJ,Park SJ,Kim CY,Oh HM,Park CS,Lim YH,Kim D,Rho MC,Lee WS,Kim YM

doi

10.1016/j.enzmictec.2014.09.001

subject

Has Abstract

pub_date

2014-12-01 00:00:00

pages

59-66

eissn

0141-0229

issn

1879-0909

pii

S0141-0229(14)00159-8

journal_volume

67

pub_type

杂志文章

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