Oxygen transfer and uptake rates during xanthan gum production.

abstract：:Photothermal therapy (PTT) is a minimally invasive and effective cancer treatment method and has a great potential for innovating the conventional chemotherapy approaches. Copper sulfide (CuS) exhibits photostability, low cost, and high absorption in near infrared region, and is recognized as an ideal candidate for PT...

journal_title：Enzyme and microbial technology

authors： Zhou NQ,Tian LJ,Wang YC,Li DB,Li PP,Zhang X,Yu HQ

更新日期：2016-12-01 00:00:00

abstract：:The mechanical properties of TB/C3 hybridoma cells taken from a continuous culture were measured by micromanipulation. The culture conditions were constant except for the presence or absence of Pluronic F-68 in the medium. It was found that the mean bursting membrane tension and the mean elastic area compressibility m...

journal_title：Enzyme and microbial technology

authors： Zhang Z,al-Rubeai M,Thomas CR

更新日期：1992-12-01 00:00:00

abstract：:We previously constructed three recombinant phyA mutant strains (PP-NPm-8, PP-NPep-6A and I44E/T252R-PhyA), showing improved catalytic efficiency or thermostability of Aspergillus niger N25 phytase, by error-prone PCR or site-directed mutagenesis. In this study, directed evolution and site-directed mutagenesis were fu...

journal_title：Enzyme and microbial technology

authors： Tang Z,Jin W,Sun R,Liao Y,Zhen T,Chen H,Wu Q,Gou L,Li C

更新日期：2018-01-01 00:00:00

abstract：:GH11 xylanase XynJ from Bacillus sp. strain 41M-1 has a β-jellyroll fold composed of eight β strands with a deep active-site cleft. We hypothesized that the thermostability of XynJ will increase if the flexibility of the β strands in the jellyroll structure is decreased without impairing activity. To verify this hypot...

journal_title：Enzyme and microbial technology

authors： Takita T,Nakatani K,Katano Y,Suzuki M,Kojima K,Saka N,Mikami B,Yatsunami R,Nakamura S,Yasukawa K

更新日期：2019-11-01 00:00:00

abstract：:Turanose is a natural isomer of sucrose. It is an emerging functional sweetener of the next generation. Turanose is catalytically synthesized from the sucrose biomass by employing amylosucrase enzyme. In this study, a novel gene encoding amylosucrase (Asmet) has been identified from the metagenome of a thermal aquatic...

journal_title：Enzyme and microbial technology

authors： Agarwal N,Narnoliya LK,Singh SP

更新日期：2019-12-01 00:00:00

abstract：:To increase the efficiency of enzyme cocktails in deconstructing cellulose and hemicelluloses present in the plant cell wall, a combination of enzymes with complementary activities is required. Xylan is the main hemicellulose component of energy crops and for its complete hydrolysis a system consisting of several enzy...

journal_title：Enzyme and microbial technology

authors： Cintra LC,da Costa IC,de Oliveira ICM,Fernandes AG,Faria SP,Jesuíno RSA,Ravanal MC,Eyzaguirre J,Ramos LP,de Faria FP,Ulhoa CJ

更新日期：2020-02-01 00:00:00

abstract：:The xylanase R8 gene (xynR8) from uncultured rumen fungi was cloned and successfully expressed in Lactobacillus reuteri. A xylanase activity of 132.1 U/mL was found in the broth of L. reuteri R8, the transformant containing pNZ3004 vector with xynR8 gene insertion. Two distinct forms of recombinant xylanase with diffe...

journal_title：Enzyme and microbial technology

authors： Cheng HL,Hu CY,Lin SH,Wang JY,Liu JR,Chen YC

更新日期：2014-10-01 00:00:00

abstract：:Hydrolysis of lignocellulosic biomass depends on the concerted actions of cellulases and accessory proteins. In this work we examined the combined action of two auxiliary proteins from the brown rot fungus Gloeophyllum trabeum: a family AA9 lytic polysaccharide monooxygenase (GtLPMO) and a GH10 xylanase (GtXyn10A). Th...

journal_title：Enzyme and microbial technology

authors： Sanhueza C,Carvajal G,Soto-Aguilar J,Lienqueo ME,Salazar O

更新日期：2018-06-01 00:00:00

abstract：:Phenylalanine ammonia-lyase (PAL) gene plays an important role in the synthesis of flavones, lignin, and other bioactive compounds in living organisms. Inonotus baumii, the only known flavone-producing filamentous fungus, is of great importance in the investigation of flavone metabolic pathways. To study the function ...

journal_title：Enzyme and microbial technology

authors： Lin W,Liu A,Weng C,Li H,Sun S,Song A,Zhu H

更新日期：2018-05-01 00:00:00

abstract：:Di-, tri-, and tetra-tyrosine peptides with angiotensin I-converting enzyme inhibitory activity were synthesized by papain-catalyzed polymerization of L-tyrosine ethyl ester in aqueous media at 30 °C. Varying the reaction pH from 6.0 to 7.5 and the initial concentration of the ester substrate from 25 to 100 mM, the hi...

journal_title：Enzyme and microbial technology

authors： Mitsuhashi J,Nakayama T,Narai-Kanayama A

更新日期：2015-07-01 00:00:00

abstract：:A reactive water-soluble polymer was synthesized by copolymerizing N-isopropylacrylamide and glycidyl acrylate. The reactive polymer could react with the amino groups of enzymes/proteins or other ligands to form an affinity polymer. As a model, the reactive polymer was allowed to react with paraaminobenzamidine, a str...

journal_title：Enzyme and microbial technology

authors： Nguyen AL,Luong JH

更新日期：1990-09-01 00:00:00

abstract：:Recently enzymatic catalysts have replaced organic and organometallic catalysts in the synthesis of bio-resorbable polymers. Enzymatic polymerization is considered as an alternative to conventional polymerization as they are less toxic, environmental friendly and can operate under mild conditions. In this research, th...

journal_title：Enzyme and microbial technology

authors： Pakalapati H,Tariq MA,Arumugasamy SK

更新日期：2019-03-01 00:00:00

abstract：:The lipases from Thermomyces lanuginosus and Pseudomonas cepacia have been immobilized on octyl and cyanogen bromide (CNBr) agarose beads. The immobilization on octyl-agarose is slowed with increasing ionic strength, while the immobilization on CNBr is not significantly affected by the ionic strength. The inhibition o...

journal_title：Enzyme and microbial technology

authors： Manoel EA,Dos Santos JC,Freire DM,Rueda N,Fernandez-Lafuente R

更新日期：2015-04-01 00:00:00

abstract：:Astragalin (kaempferol-3-O-β-D-glucopyranoside, Ast) glucosides were synthesized by the acceptor reaction of a dextransucrase produced by Leuconostoc mesenteroides B-512FMCM with astragalin and sucrose. Each glucoside was purified and their structures were assigned as kaempferol-3-O-β-D-glucopyranosyl-(1→3)-O-α-D-gluc...

journal_title：Enzyme and microbial technology

authors： Kim GE,Kang HK,Seo ES,Jung SH,Park JS,Kim DH,Kim DW,Ahn SA,Sunwoo C,Kim D

更新日期：2012-01-05 00:00:00

abstract：:The hydrolytic activity of a thermophilic cyclomaltodextrinase (CMD) from Anoxybacillus flavithermus ZNU-NGA and a representative single mutant were investigated against soluble substrates including α-, β- and γ-cyclomaltodestrines (CDs). Based on the occurrence of arginine (Arg) at position 403 in some homologue prot...

journal_title：Enzyme and microbial technology

authors： Mirzaee Z,Jafarian V,Shirdel SA,Khalifeh K

更新日期：2019-12-01 00:00:00

abstract：:The different physical and chemical properties of lignin might have various effects on the enzymatic hydrolysis of lignocellulosic substrates. In this study, the influence of lignin on enzymatic digestibility of cellulose was assessed. Addition of 20% (4g/L) isolated enzymatic lignin (lignin 2 and 3) and kraft lignin ...

journal_title：Enzyme and microbial technology

authors： Zhang H,Wu S,Xie J

更新日期：2017-06-01 00:00:00

abstract：:The effect of treatment with various intercalating dyes on the ability to produce antibiotics in Micromonospora rosaria and Micromonospora purpurea was studied. Treatment with acriflavine resulted in a high frequency loss of antibiotic productivity in both species. In M. rosaria, the loss of antibiotic-producing abili...

journal_title：Enzyme and microbial technology

authors： Kim KS,Pai HS,Lee SY,Ryu DD

更新日期：1990-08-01 00:00:00

abstract：:A method to stably immobilize microbes on electrodes was developed. Resting cells of Methylobacterium extorquens AM1(MeAM1) were caged within multiwalled carbon nanotubes (MWNTs)by adding the cells to a water dispersion of MWNTs then allowing the resulting mixture to dry on electrodes. The MeAM1-MWCNTs electrode thus ...

journal_title：Enzyme and microbial technology

authors： Xia HQ,Sakai K,Kitazumi Y,Shirai O,Takagi K,Kano K

更新日期：2018-10-01 00:00:00

abstract：:Gamma glutamyl transpeptidase from Bacillus pumilus KS12 (GGTBP) was cloned, expressed in pET-28-E. coli expression system as a heterodimeric enzyme with molecular weights of 45 and 20 kDa for large and small subunit, respectively. It was purified by nickel affinity chromatography with hydrolytic and transpeptidase ac...

journal_title：Enzyme and microbial technology

authors： Murty NA,Tiwary E,Sharma R,Nair N,Gupta R

更新日期：2012-03-10 00:00:00

abstract：:A new bi-modular, wide pH spectrum and highly active xylanase KRICT PX3 (JF320814) isolated from Paenibacillus terrae HPL-003 (KCTC11987BP) has been cloned and expressed in Escherichia coli. Purified recombinant xylanase KRICT PX-3 (1,620 bp, 540aa, NCBI accession number JF320814) showed highly active at 55°C in pH 4....

journal_title：Enzyme and microbial technology

authors： Song HY,Lim HK,Kim DR,Lee KI,Hwang IT

更新日期：2014-01-10 00:00:00

abstract：:A three-phase fluidized bed reactor (TPFBR) was designed to evaluate the potential of CoQ(10) production by gel-entrapped Sphingomonas sp. ZUTE03 via a conversion-extract coupled process. In the reactor, the CoQ(10) yield reached 46.99 mg/L after 8 h of conversion; a high-level yield of about 45 mg/L was maintained ev...

journal_title：Enzyme and microbial technology

authors： Qiu L,Ding H,Wang W,Kong Z,Li X,Shi Y,Zhong W

更新日期：2012-02-10 00:00:00

abstract：:A novel endoglucanase active on 1,3-1,4-beta-D-glucans was purified to apparent homogeneity from submerged cultures of the moderately thermophilic aerobic fungus Talaromyces emersonii CBS 814.70. The enzyme is a single subunit glycoprotein with M(r) and pI values of 40.7 +/- 0.3 kDa and 4.4, respectively, and an estim...

journal_title：Enzyme and microbial technology

authors： Murray PG,Grassick A,Laffey CD,Cuffe MM,Higgins T,Savage AV,Planas A,Tuohy MG

更新日期：2001-07-05 00:00:00

abstract：:Crocin, one of the major carotenoid pigments of Crocus sativus (saffron), is responsible for antioxidant activity, neuroprotection, and the inhibition of tumor cell proliferation. In order to improve the functionality of crocin, α-glucosyl-(1→6)-trans-crocins (C-Gs) were synthesized using sucrose and dextransucrase fr...

journal_title：Enzyme and microbial technology

authors： Mok IK,Nguyen TTH,Kim DH,Lee JW,Lim S,Jung HY,Lim T,Pal K,Kim D

更新日期：2020-10-01 00:00:00

abstract：:Escherichia coli B/pTG201 recombinant cells were immobilized by entrapment in a carrageenan gel and cultivated in nonselective media to investigate the effect of agitation rate on plasmid stability, biomass concentration, and enzyme productivity. These parameters were studied in continuous cultures for free and immobi...

journal_title：Enzyme and microbial technology

authors： Huang J,Dhulster P,Thomas D,Barbotin JN

更新日期：1990-12-01 00:00:00

abstract：:2,5-Bis(hydroxymethyl)furan (BHMF) is a versatile building block in the synthesis of polymers, fuels, and macrocycle polyethers. In this work, alcohol dehydrogenases (ADHs) were identified from Meyerozyma guilliermondii SC1103 and were heterologously expressed in Saccharomyces cerevisiae for the synthesis of BHMF from...

journal_title：Enzyme and microbial technology

authors： Xia ZH,Zong MH,Li N

更新日期：2020-03-01 00:00:00

abstract：:The activities (at pH 7 and 50 degrees C) of purified EGV (Humicola insolens) and CenA (Cellulomonas fimi) were determined on cotton fabrics at high and low levels of mechanical agitation. Similar activity measurements were also made by using the core domains of these cellulases. Activity experiments suggested that th...

journal_title：Enzyme and microbial technology

authors： Azevedo H,Bishop D,Cavaco-Paulo A

更新日期：2000-08-01 00:00:00

abstract：:We here describe a unique β-D-glucosidase (BGL; Blon_0625) derived from Bifidobacterium longum subsp. infantis ATCC 15697. The Blon_0625 gene was expressed by recombinant Escherichia coli. Purified recombinant Blon_0625 retains hydrolyzing activity against both p-nitrophenyl-β-D-glucopyranoside (pNPG; 17.3±0.24Umg(-1)...

journal_title：Enzyme and microbial technology

authors： Matsumoto T,Shimada S,Hata Y,Tanaka T,Kondo A

更新日期：2015-01-01 00:00:00

abstract：:Two GH43 β-xylosidases, RS223-BX from a rice straw metagenomic library, and BoXA from Bacteroides ovatus, that share similar amino acid sequences (81% identical) and 19 of 20 active-site residues, were compared by using site-directed mutagenesis of Asp and His residues implicated in metal binding. Thus, RS223-BX is st...

journal_title：Enzyme and microbial technology

authors： Jordan DB,Stoller JR,Kibblewhite RE,Chan VJ,Lee CC,Wagschal K

更新日期：2018-07-01 00:00:00

abstract：:Addition of non-ionic surfactants (NIS) is known to accelerate enzymatic lignocellulose hydrolysis. The mechanism behind this accelerating effect is still not elucidated but has been hypothesized to originate from favorable NIS-lignin interactions which alleviate non-productive adsorption of cellulases to lignin. In t...

journal_title：Enzyme and microbial technology

authors： Olsen SN,Bohlin C,Murphy L,Borch K,McFarland KC,Sweeny MD,Westh P

更新日期：2011-09-10 00:00:00

abstract：:Foaming, a proficient method for the recovery of surface active solutes from dilute solutions, was successfully applied for the concentration of the lipopeptide biosurfactant Surfactin from B. subtilis ATCC 21332 cell culture broths. Foaming was only partially successful in concentrating Surfactin when applied as a se...

journal_title：Enzyme and microbial technology

authors： Davis DA,Lynch HC,Varley J

更新日期：2001-03-08 00:00:00