Abstract:
:Addition of non-ionic surfactants (NIS) is known to accelerate enzymatic lignocellulose hydrolysis. The mechanism behind this accelerating effect is still not elucidated but has been hypothesized to originate from favorable NIS-lignin interactions which alleviate non-productive adsorption of cellulases to lignin. In the current work we address this hypothesis using tannic acid (TAN) as a general poly-phenolic model compound (for lignin and soluble phenolics) and measure the mutual interactions of cellulases (CBHI, CBHII, EGI, EGII and BG), TAN and NIS (Triton X-100) using isothermal titration calorimetry (ITC). The experimental results suggest rather strong enzyme-specific interactions with TAN in reasonable agreement with enzyme specific lignin inhibition found in the literature. Enzyme-TAN interactions were disrupted by the presence of NIS by a mechanism of strong TAN-NIS interaction. The presence of NIS also alleviated the inhibitory effect of TAN on cellulase activity. All together the current work provides strong indications that favorable NIS-poly-phenol interactions alleviate non-productive cellulase-poly-phenol interactions and hence may provide a mechanism for the accelerating effect of NIS on lignocellulose hydrolysis.
journal_name
Enzyme Microb Technoljournal_title
Enzyme and microbial technologyauthors
Olsen SN,Bohlin C,Murphy L,Borch K,McFarland KC,Sweeny MD,Westh Pdoi
10.1016/j.enzmictec.2011.06.015subject
Has Abstractpub_date
2011-09-10 00:00:00pages
353-9issue
4eissn
0141-0229issn
1879-0909pii
S0141-0229(11)00132-3journal_volume
49pub_type
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