Abstract：
:2,5-Bis(hydroxymethyl)furan (BHMF) is a versatile building block in the synthesis of polymers, fuels, and macrocycle polyethers. In this work, alcohol dehydrogenases (ADHs) were identified from Meyerozyma guilliermondii SC1103 and were heterologously expressed in Saccharomyces cerevisiae for the synthesis of BHMF from 5-hydroxymethylfurfural (HMF). Of recombinant strains constructed, S. cerevisiae expressing an aryl ADH (MgAAD1669) was observed to be the best catalyst. Upon process optimization, BHMF was afforded with a 99% selectivity and a 94% yield within 24 h at the substrate concentration of 250 mM. The space-time yield up to 3.4 g/L h was achieved in the fed-batch synthesis of BHMF. Inexpensive corncob hydrolysate proved to be a promising alternative to glucose as co-substrate for biocatalytic synthesis of BHMF, thus resulting in the significantly reduced production cost.
journal_name
Enzyme Microb Technoljournal_title
Enzyme and microbial technologyauthors
Xia ZH,Zong MH,Li Ndoi
10.1016/j.enzmictec.2019.109491subject
Has Abstractpub_date
2020-03-01 00:00:00pages
109491eissn
0141-0229issn
1879-0909pii
S0141-0229(19)30229-7journal_volume
134pub_type
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journal_title：Enzyme and microbial technology
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journal_title：Enzyme and microbial technology
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journal_title：Enzyme and microbial technology
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journal_title：Enzyme and microbial technology
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journal_title：Enzyme and microbial technology
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journal_title：Enzyme and microbial technology
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journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/j.enzmictec.2012.03.012
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journal_title：Enzyme and microbial technology
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journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/j.enzmictec.2017.04.009
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journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/j.enzmictec.2020.109651
更新日期：2020-11-01 00:00:00
abstract：:Photothermal therapy (PTT) is a minimally invasive and effective cancer treatment method and has a great potential for innovating the conventional chemotherapy approaches. Copper sulfide (CuS) exhibits photostability, low cost, and high absorption in near infrared region, and is recognized as an ideal candidate for PT...
journal_title：Enzyme and microbial technology
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doi：10.1016/j.enzmictec.2016.04.002
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journal_title：Enzyme and microbial technology
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journal_title：Enzyme and microbial technology
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journal_title：Enzyme and microbial technology
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journal_title：Enzyme and microbial technology
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journal_title：Enzyme and microbial technology
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abstract：:The mass distribution regularity in substrate of solid-state fermentation (SSF) has rarely been reported due to the heterogeneity of solid medium and the lack of suitable instrument and method, which limited the comprehensive analysis and enhancement of the SSF performance. In this work, the distributions of water, bi...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/j.enzmictec.2014.02.007
更新日期：2014-05-10 00:00:00
abstract：:Zygosaccharomyces rouxii is an important yeast in the formation of flavor in soy sauce. In this study, we investigated the separate effects of exogenous threonine, cystathionine, and the branched-chain amino acids on the metabolism of Z. rouxii. The addition of these amino acids had significant effects on both Z. roux...
journal_title：Enzyme and microbial technology
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doi：10.1016/s0141-0229(99)00165-9
更新日期：2000-02-01 00:00:00
abstract：:Conductive materials with attached biofilms, were used as membrane filtration biocathodes to filter the effluent and supply electrons for denitrification. Stainless steel mesh and carbon felt were employed to fabricate membrane modules, and the two MFC systems were termed as M1 and M2, respectively. High effluent qual...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/j.enzmictec.2014.04.005
更新日期：2014-06-10 00:00:00
abstract：:In this study, distillery wastewater was treated by dark fermentation or photofermentation alone, and by sequential dark and photofermentation processes using anaerobic saccharolytic consortium and purple nonsulfur bacteria. Combination of dark and photofermentation resulted in the maximal H2 yield of 17.6L/L of disti...
journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/j.enzmictec.2017.11.009
更新日期：2018-03-01 00:00:00
