Catalytic synthesis of 2,5-bis(hydroxymethyl)furan from 5-hydroxymethylfurfual by recombinant Saccharomyces cerevisiae.


:2,5-Bis(hydroxymethyl)furan (BHMF) is a versatile building block in the synthesis of polymers, fuels, and macrocycle polyethers. In this work, alcohol dehydrogenases (ADHs) were identified from Meyerozyma guilliermondii SC1103 and were heterologously expressed in Saccharomyces cerevisiae for the synthesis of BHMF from 5-hydroxymethylfurfural (HMF). Of recombinant strains constructed, S. cerevisiae expressing an aryl ADH (MgAAD1669) was observed to be the best catalyst. Upon process optimization, BHMF was afforded with a 99% selectivity and a 94% yield within 24 h at the substrate concentration of 250 mM. The space-time yield up to 3.4 g/L h was achieved in the fed-batch synthesis of BHMF. Inexpensive corncob hydrolysate proved to be a promising alternative to glucose as co-substrate for biocatalytic synthesis of BHMF, thus resulting in the significantly reduced production cost.


Enzyme Microb Technol


Xia ZH,Zong MH,Li N




Has Abstract


2020-03-01 00:00:00












  • Enhancement of neuroprotection, antioxidant capacity, and water-solubility of crocins by transglucosylation using dextransucrase under high hydrostatic pressure.

    abstract::Crocin, one of the major carotenoid pigments of Crocus sativus (saffron), is responsible for antioxidant activity, neuroprotection, and the inhibition of tumor cell proliferation. In order to improve the functionality of crocin, α-glucosyl-(1→6)-trans-crocins (C-Gs) were synthesized using sucrose and dextransucrase fr...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Mok IK,Nguyen TTH,Kim DH,Lee JW,Lim S,Jung HY,Lim T,Pal K,Kim D

    更新日期:2020-10-01 00:00:00

  • UV-Vis detection of hydrogen peroxide using horseradish peroxidase/copper phosphate hybrid nanoflowers.

    abstract::A colorimetric platform based on enzyme-inorganic hybrid nanoflowers was used for ultraviolet-visible detection of hydrogen peroxide (H2O2). The hybrid nanoflowers were prepared via incubation at room temperature using horseradish peroxidase (HRP) and copper phosphate (Cu3(PO4)2) as the organic and inorganic component...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Yang C,Zhang M,Wang W,Wang Y,Tang J

    更新日期:2020-10-01 00:00:00

  • Aspergillus niger I-1472 and Pycnoporus cinnabarinus MUCL39533, selected for the biotransformation of ferulic acid to vanillin, are also able to produce cell wall polysaccharide-degrading enzymes and feruloyl esterases.

    abstract::The filamentous fungal strains Aspergillus niger I-1472 and Pycnoporus cinnabarinus MUCL39533, previously selected for the bioconversion of ferulic acid to vanillic acid and vanillin respectively, were grown on sugar beet pulp. A large spectrum of polysaccharide-degrading enzymes was produced by A. niger and very few ...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Bonnina E,Brunel M,Gouy Y,Lesage-Meessen L,Asther M,Thibault J

    更新日期:2001-01-02 00:00:00

  • Synthesis of long-chain alkyl glucosides via reverse hydrolysis reactions catalyzed by an engineered β-glucosidase.

    abstract::Long-chain alkyl glucosides, such as octyl and decyl β-d-glucopyranosides (OG and DG, respectively), are regarded as a new generation of biodegradable, non-ionic surfactants. Previously, the mutants of Dalbergia cochinchinensis Pierre dalcochinase showed potential in the synthesis of oligosaccharides and alkyl glucosi...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Thenchartanan P,Pitchayatanakorn P,Wattana-Amorn P,Ardá A,Svasti J,Jiménez-Barbero J,Kongsaeree PT

    更新日期:2020-10-01 00:00:00

  • Mechanism of papain-catalyzed synthesis of oligo-tyrosine peptides.

    abstract::Di-, tri-, and tetra-tyrosine peptides with angiotensin I-converting enzyme inhibitory activity were synthesized by papain-catalyzed polymerization of L-tyrosine ethyl ester in aqueous media at 30 °C. Varying the reaction pH from 6.0 to 7.5 and the initial concentration of the ester substrate from 25 to 100 mM, the hi...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Mitsuhashi J,Nakayama T,Narai-Kanayama A

    更新日期:2015-07-01 00:00:00

  • Growth of 'Candidatus Liberibacter asiaticus' in a host-free microbial culture is associated with microbial community composition.

    abstract::'Candidatus Liberibacter asiaticus' ('Ca. L. asiaticus'), the suspected causative agent of citrus greening disease, is one of many phloem-restricted plant pathogens that have not been isolated and grown in an axenic culture. In this study, infected Asian citrus psyllids were used to prepare a host-free source of 'Ca. ...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Molki B,Call DR,Ha PT,Omsland A,Gang DR,Lindemann SR,Killiny N,Beyenal H

    更新日期:2020-12-01 00:00:00

  • Production of d-glucuronic acid from myo-inositol using Escherichia coli whole-cell biocatalyst overexpressing a novel myo-inositol oxygenase from Thermothelomyces thermophile.

    abstract::D-glucuronic acid (GlcUA) is an important intermediate with numerous applications in the food, cosmetics, and pharmaceutical industries. Its biological production routes which employ myo-inositol oxygenase (MIOX) as the key enzyme are attractive. In this study, five diverse MIOX-encoding genes, from Cryptococcus neofo...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Teng F,You R,Hu M,Liu W,Wang L,Tao Y

    更新日期:2019-08-01 00:00:00

  • The use of oxygen uptake rate measurements to control the supply of toxic substrate: toluene hydroxylation by Pseudomonas putida UV4.

    abstract::During toluene hydroxylation, catalyzed by Pseudomonas putida UV4 one molecule of oxygen is added to the aromatic ring to produce the dihydroxylated (non-aromatic) ring structure, toluene cis-glycol. Toluene, which is toxic to the cells at aqueous phase concentration above ( approximately 2.4 mmol), is fed to the reac...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Carragher JM,McClean WS,Woodley JM,Hack CJ

    更新日期:2001-02-01 00:00:00

  • Purification and characterization of an extracellular alpha-amylase produced by Lactobacillus manihotivorans LMG 18010(T), an amylolytic lactic acid bacterium.

    abstract::This work presents the purification and characterization of an extracellular alpha-amylase (1,4-alpha-D-glucan glucanohydrolase, EC produced by a new lactic acid bacterium: Lactobacillus manihotivorans able to produce L(+) lactic acid from starch. The molecular weight was found to be 135 kDa. The temperature ...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Aguilar G,Morlon-Guyot J,Trejo-Aguilar B,Guyot JP

    更新日期:2000-09-01 00:00:00

  • Studies on the purification of peroxidase from horseradish roots using reverse micelles.

    abstract::Horseradish peroxidase (HRP) was successfully purified from horseradish roots by a two-stage reverse-micellar extraction from the dialyzed aqueous extract. The anionic surfactant AOT dissolved in isooctane was used to produce the reverse-micellar phases. The narrow pH range at which HRP solubilization occurred was exp...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Regalado C,Asenjo JA,Pyle DL

    更新日期:1996-04-01 00:00:00

  • Synthesis of esters using a nylon-immobilized lipase in batch and continuous reactors.

    abstract::Direct esterifications using a nylon-immobilized lipase from Candida cylindracea were carried out in batch and continuous-flow reactors. The immobilized enzyme was effective in catalyzing the synthesis of ethylpropionate, isoamylpropionate, and isoamylbutyrate. With ethanol dissolved in hexane as a substrate, the maxi...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Carta G,Gainer JL,Gibson ME

    更新日期:1992-11-01 00:00:00

  • ZnS quantum dots as pH probes for study of enzyme reaction kinetics.

    abstract::Water soluble ZnS quantum dots (QDs) modified by mercaptoacetic acid (MAA) were used to determinate proton concentration in aqueous solutions by fluorescence spectroscopic technique. The results showed that the fluorescence of the water-soluble QDs could be quenched by proton concentration and the fluorescence intensi...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Wu D,Chen Z

    更新日期:2012-06-10 00:00:00

  • DNase activity of micrococcal endonuclease covalently immobilized on nylon and polystyrene.

    abstract::Water-insoluble nucleases were prepared by immobilizing the endonuclease from S. aureus onto the surface of nylon-66 and polystyrene spheres. The activation phase of the synthetic supports was optimized to define optimal conditions of pH, temperature, and Ca2+ concentration for using immobilized enzymes. The activity,...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Michalon P,Roche J,Couturier R,Favre-Bonvin G,Marion C

    更新日期:1993-03-01 00:00:00

  • Engineering and optimization of phosphate-responsive phytase expression in Pichia pastoris yeast for phytate hydrolysis.

    abstract::Phytate is the major storage form of phosphorus in plants. It is present in cereals and raw materials of vegetable origin used in animal and human diets. However, non-ruminant animals have little phytase activity in their guts and, therefore, cannot digest phytate. As a result, almost all dietary phytate is discharged...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Xie Z,Fong WP,Tsang PW

    更新日期:2020-06-01 00:00:00

  • Overexpression of hlyB and mdh genes confers halotolerance in Fremyella diplosiphon, a freshwater cyanobacterium.

    abstract::Fremyella diplosiphon is a freshwater cyanobacterium that has great potential as a biofuel agent due to its ability to grow in low light intensity and acclimation to different wavelengths. To enhance its halotolerance for growth in 35gL-1 sodium chloride (NaCl), plasmids harboring hemolysin B (hlyB) and malate dehydro...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Tabatabai B,Arumanayagam AS,Enitan O,Mani A,Natarajan SS,Sitther V

    更新日期:2017-08-01 00:00:00

  • Identification, repair and characterization of a benzyl alcohol-inducible promoter for recombinant proteins overexpression in Corynebacterium glutamicum.

    abstract::Corynebacterium glutamicum is an important industrial organism for the production of a variety of biological commodities. We discovered a promoter encoded by the gene NCgl2319 in C. glutamicum, which could be induced by benzyl alcohol, could be used as an efficient tunable expression system. In initial attempts, this ...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Liu X,Zhao Z,Dong G,Li Y,Peng F,Liu C,Zhang F,Linhardt RJ,Yang Y,Bai Z

    更新日期:2020-11-01 00:00:00

  • Extracellular biosynthesis of copper sulfide nanoparticles by Shewanella oneidensis MR-1 as a photothermal agent.

    abstract::Photothermal therapy (PTT) is a minimally invasive and effective cancer treatment method and has a great potential for innovating the conventional chemotherapy approaches. Copper sulfide (CuS) exhibits photostability, low cost, and high absorption in near infrared region, and is recognized as an ideal candidate for PT...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Zhou NQ,Tian LJ,Wang YC,Li DB,Li PP,Zhang X,Yu HQ

    更新日期:2016-12-01 00:00:00

  • Improved thermostability and enzyme activity of a recombinant phyA mutant phytase from Aspergillus niger N25 by directed evolution and site-directed mutagenesis.

    abstract::We previously constructed three recombinant phyA mutant strains (PP-NPm-8, PP-NPep-6A and I44E/T252R-PhyA), showing improved catalytic efficiency or thermostability of Aspergillus niger N25 phytase, by error-prone PCR or site-directed mutagenesis. In this study, directed evolution and site-directed mutagenesis were fu...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Tang Z,Jin W,Sun R,Liao Y,Zhen T,Chen H,Wu Q,Gou L,Li C

    更新日期:2018-01-01 00:00:00

  • A thermophilic enzymatic cocktail for galactomannans degradation.

    abstract::The full utilization of hemicellulose sugars (pentose and exose) present in lignocellulosic material, is required for an efficient bio-based fuels and chemicals production. Two recombinant thermophilic enzymes, an endo-1,4-β-mannanase from Dictyoglomus turgidum (DturCelB) and an α-galactosidase from Thermus thermophil...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Aulitto M,Fusco FA,Fiorentino G,Bartolucci S,Contursi P,Limauro D

    更新日期:2018-04-01 00:00:00

  • Effects of agitation level on the adsorption, desorption, and activities on cotton fabrics of full length and core domains of EGV (Humicola insolens) and CenA (Cellulomonas fimi).

    abstract::The activities (at pH 7 and 50 degrees C) of purified EGV (Humicola insolens) and CenA (Cellulomonas fimi) were determined on cotton fabrics at high and low levels of mechanical agitation. Similar activity measurements were also made by using the core domains of these cellulases. Activity experiments suggested that th...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Azevedo H,Bishop D,Cavaco-Paulo A

    更新日期:2000-08-01 00:00:00

  • Chain length selectivity during the polycondensation of siloxane-containing esters and alcohols by immobilized Candida antarctica lipase B.

    abstract::We have examined the chain length selectivity for a series of acyl donors by lipase B from Candida antarctica (CalB). CalB accepted aliphatic diesters of C4, C6 and C12 chain lengths equally. The introduction of a carbon-carbon double bond into the C4 esters dramatically lowered the rate constant associated with polym...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Frampton MB,Zelisko PM

    更新日期:2014-05-10 00:00:00

  • Electrochemical detection of Pseudomonas aeruginosa 16S rRNA using a biosensor based on immobilized stem-loop structured probe.

    abstract::We have designed an electrochemical DNA biosensor based on stem-loop structured probes for enzymatic detection of Pseudomonas aeruginosa 16S ribosomal RNA (rRNA) in composting degradation. The probe modified with a thiol at its 5' end and a biotin at its 3' end was immobilized on a gold electrode through self-assembly...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Liu C,Zeng GM,Tang L,Zhang Y,Li YP,Liu YY,Li Z,Wu MS,Luo J

    更新日期:2011-08-10 00:00:00

  • Characterization of two acidic β-glucosidases and ethanol fermentation in the brown rot fungus Fomitopsis palustris.

    abstract::Two acidic β-glucosidases (βGI and βGII) from the brown rot fungus Fomitopsis palustris were purified to homogeneity by several chromatographic steps. βGI and βGII had molecular weights of 130 and 213 kDa, respectively, and exhibited optimum activity at pH 2.5 and 55°C. The K(m) values of βGI and βGII for p-nitropheny...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Okamoto K,Sugita Y,Nishikori N,Nitta Y,Yanase H

    更新日期:2011-04-07 00:00:00

  • Stabilization of Penicillium occitanis cellulases by spray drying in presence of Maltodextrin.

    abstract::The stabilization of fungal cellulases by spray drying, the thermal stability of Penicillium occitanis cellulases and the effect of some additives were studied. We observed that CMCase activity presents a good stability at 50 degrees C, even after 60 h of incubation. On the other hand, beta-glucosidase activity was mo...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Belghith H,Ellouz Chaabouni S,Gargouri A

    更新日期:2001-02-01 00:00:00

  • Oxygen derepresses deacetoxycephalosporin C synthase and increases the conversion of penicillin N to cephamycin C in Streptomyces clavuligerus.

    abstract::When dissolved oxygen (DO) was maintained at saturation level during batch fermentations of Streptomyces clavuligerus (NRRL 3585), the accumulation of the intermediate penicillin N was lowered while formation of the end product cephamycin C was increased relative to fermentations without DO control. The specific activ...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Rollins MJ,Jensen SE,Wolfe S,Westlake DW

    更新日期:1990-01-01 00:00:00

  • Conversion of cysteine to 3-mercaptopyruvic acid by bacterial aminotransferases.

    abstract::3-Mercaptopyruvate (3MPy), a structural analog of 3-mercaptopropionic acid, is a precursor compound for biosynthesis of polythioesters in bacteria. The cost-effectiveness and sustainability of the whole process could be greatly improved by using the cysteine degradation pathway for an intracellular supply of 3MPy. Tra...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Andreeßen C,Gerlt V,Steinbüchel A

    更新日期:2017-04-01 00:00:00

  • The effect of gas double-dynamic on mass distribution in solid-state fermentation.

    abstract::The mass distribution regularity in substrate of solid-state fermentation (SSF) has rarely been reported due to the heterogeneity of solid medium and the lack of suitable instrument and method, which limited the comprehensive analysis and enhancement of the SSF performance. In this work, the distributions of water, bi...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Chen HZ,Zhao ZM,Li HQ

    更新日期:2014-05-10 00:00:00

  • Effect of threonine, cystathionine, and the branched-chain amino acids on the metabolism of Zygosaccharomyces rouxii*

    abstract::Zygosaccharomyces rouxii is an important yeast in the formation of flavor in soy sauce. In this study, we investigated the separate effects of exogenous threonine, cystathionine, and the branched-chain amino acids on the metabolism of Z. rouxii. The addition of these amino acids had significant effects on both Z. roux...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: van der Sluis C,Wolken WA,Giuseppin ML,Tramper J,Wijffels RH

    更新日期:2000-02-01 00:00:00

  • Membrane filtration biocathode microbial fuel cell for nitrogen removal and electricity generation.

    abstract::Conductive materials with attached biofilms, were used as membrane filtration biocathodes to filter the effluent and supply electrons for denitrification. Stainless steel mesh and carbon felt were employed to fabricate membrane modules, and the two MFC systems were termed as M1 and M2, respectively. High effluent qual...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Zhang G,Zhang H,Ma Y,Yuan G,Yang F,Zhang R

    更新日期:2014-06-10 00:00:00

  • Utilization of distillery wastewater for hydrogen production in one-stage and two-stage processes involving photofermentation.

    abstract::In this study, distillery wastewater was treated by dark fermentation or photofermentation alone, and by sequential dark and photofermentation processes using anaerobic saccharolytic consortium and purple nonsulfur bacteria. Combination of dark and photofermentation resulted in the maximal H2 yield of 17.6L/L of disti...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Laurinavichene T,Tekucheva D,Laurinavichius K,Tsygankov A

    更新日期:2018-03-01 00:00:00