Catalytic synthesis of 2,5-bis(hydroxymethyl)furan from 5-hydroxymethylfurfual by recombinant Saccharomyces cerevisiae.


:2,5-Bis(hydroxymethyl)furan (BHMF) is a versatile building block in the synthesis of polymers, fuels, and macrocycle polyethers. In this work, alcohol dehydrogenases (ADHs) were identified from Meyerozyma guilliermondii SC1103 and were heterologously expressed in Saccharomyces cerevisiae for the synthesis of BHMF from 5-hydroxymethylfurfural (HMF). Of recombinant strains constructed, S. cerevisiae expressing an aryl ADH (MgAAD1669) was observed to be the best catalyst. Upon process optimization, BHMF was afforded with a 99% selectivity and a 94% yield within 24 h at the substrate concentration of 250 mM. The space-time yield up to 3.4 g/L h was achieved in the fed-batch synthesis of BHMF. Inexpensive corncob hydrolysate proved to be a promising alternative to glucose as co-substrate for biocatalytic synthesis of BHMF, thus resulting in the significantly reduced production cost.


Enzyme Microb Technol


Xia ZH,Zong MH,Li N




Has Abstract


2020-03-01 00:00:00












  • Characterization of two acidic β-glucosidases and ethanol fermentation in the brown rot fungus Fomitopsis palustris.

    abstract::Two acidic β-glucosidases (βGI and βGII) from the brown rot fungus Fomitopsis palustris were purified to homogeneity by several chromatographic steps. βGI and βGII had molecular weights of 130 and 213 kDa, respectively, and exhibited optimum activity at pH 2.5 and 55°C. The K(m) values of βGI and βGII for p-nitropheny...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Okamoto K,Sugita Y,Nishikori N,Nitta Y,Yanase H

    更新日期:2011-04-07 00:00:00

  • Effects of growth medium composition, iron sources and atmospheric oxygen concentrations on production of luciferase-bacterial magnetic particle complex by a recombinant Magnetospirillum magneticum AMB-1.

    abstract::Growth conditions for mass production of luciferase-bacterial magnetic particles (BMPs) by a recombinant Magnetospirillum magneticum AMB-1 were investigated in a pH-regulated fed-batch culture system. Enrichment of growth medium with L-cysteine, yeast extract and polypeptone enhanced both bacterial growth and BMP prod...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Yang C,Takeyama H,Tanaka T,Matsunaga T

    更新日期:2001-07-05 00:00:00

  • Direct and selective enzymatic synthesis of trehalose unsaturated fatty acid diesters and evaluation of foaming and emulsifying properties.

    abstract::Trehalose diesters are Gemini-type surfactants that might have better surface activity than conventional surfactants. A one-step method for the preparation of trehalose unsaturated fatty acid diesters has been successfully developed. The yield of trehalose diester of different unsaturated fatty acids was between 78 % ...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Ji S,Jia C,Cao D,Li S,Zhang X

    更新日期:2020-05-01 00:00:00

  • Growth of 'Candidatus Liberibacter asiaticus' in a host-free microbial culture is associated with microbial community composition.

    abstract::'Candidatus Liberibacter asiaticus' ('Ca. L. asiaticus'), the suspected causative agent of citrus greening disease, is one of many phloem-restricted plant pathogens that have not been isolated and grown in an axenic culture. In this study, infected Asian citrus psyllids were used to prepare a host-free source of 'Ca. ...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Molki B,Call DR,Ha PT,Omsland A,Gang DR,Lindemann SR,Killiny N,Beyenal H

    更新日期:2020-12-01 00:00:00

  • Measurement of hybridoma cell number, viability, and morphology using fully automated image analysis.

    abstract::A novel automated image analysis method is described for characterizing the viability and morphology of animal cells from suspension cultures. With the aid of the exclusion dye Trypan Blue, the total and viable cell counts and the percentage of dead cells present are found. The area, perimeter, equivalent diameter, an...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Tucker KG,Chalder S,al-Rubeai M,Thomas CR

    更新日期:1994-01-01 00:00:00

  • Covalent immobilization of catalase onto spacer-arm attached modified florisil: characterization and application to batch and plug-flow type reactor systems.

    abstract::Catalase was covalently immobilized onto florisil via glutaraldehyde (GA) and glutaraldehyde+6-amino hexanoic acid (6-AHA) (as a spacer arm). Immobilizations of catalase onto modified supports were optimized to improve the efficiency of the overall immobilization procedures. The V(max) values of catalase immobilized v...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Alptekin O,Tükel SS,Yildirim D,Alagöz D

    更新日期:2011-12-10 00:00:00

  • Salt inhibition kinetics in nitrification of synthetic saline wastewater.

    abstract::Nitrification kinetics of synthetic saline wastewater was investigated by using an activated sludge unit. Initial experiments were performed with salt-free wastewater to obtain baseline information. Experiments with 3% salt concentration were performed at different sludge ages in order to investigate the system perfor...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Dinçer AR,Kargi F

    更新日期:2001-05-07 00:00:00

  • Mechanism of papain-catalyzed synthesis of oligo-tyrosine peptides.

    abstract::Di-, tri-, and tetra-tyrosine peptides with angiotensin I-converting enzyme inhibitory activity were synthesized by papain-catalyzed polymerization of L-tyrosine ethyl ester in aqueous media at 30 °C. Varying the reaction pH from 6.0 to 7.5 and the initial concentration of the ester substrate from 25 to 100 mM, the hi...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Mitsuhashi J,Nakayama T,Narai-Kanayama A

    更新日期:2015-07-01 00:00:00

  • Heterologous expression of Deinococcus geothermalis amylosucrase in Corynebacterium glutamicum for luteolin glucoside production.

    abstract::Amylosucrase (ASase) has great industrial potential owing to its multifunctional activities, including transglucosylation, polymerization, and isomerization. In the present study, the properties of Deinococcus geothermalis ASase (DGAS) expressed in Corynebacterium glutamicum (cDGAS) and purified via Ni-NTA affinity ch...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Chin YW,Jang SW,Shin HS,Kim TW,Kim SK,Park CS,Seo DH

    更新日期:2020-04-01 00:00:00

  • Coenzyme Q(10) production by immobilized Sphingomonas sp. ZUTE03 via a conversion-extraction coupled process in a three-phase fluidized bed reactor.

    abstract::A three-phase fluidized bed reactor (TPFBR) was designed to evaluate the potential of CoQ(10) production by gel-entrapped Sphingomonas sp. ZUTE03 via a conversion-extract coupled process. In the reactor, the CoQ(10) yield reached 46.99 mg/L after 8 h of conversion; a high-level yield of about 45 mg/L was maintained ev...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Qiu L,Ding H,Wang W,Kong Z,Li X,Shi Y,Zhong W

    更新日期:2012-02-10 00:00:00

  • A new strategy for strain improvement of Aurantiochytrium sp. based on heavy-ions mutagenesis and synergistic effects of cold stress and inhibitors of enoyl-ACP reductase.

    abstract::Developing a strain with high docosahexaenoic acid (DHA) yield and stable fermenting-performance is an imperative way to improve DHA production using Aurantiochytrium sp., a microorganism with two fatty acid synthesis pathways: polyketide synthase (PKS) pathway and Type I fatty acid synthase (FAS) pathway. This study ...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Cheng YR,Sun ZJ,Cui GZ,Song X,Cui Q

    更新日期:2016-11-01 00:00:00

  • A novel method for simultaneous purification and immobilization of a xylanase-lichenase chimera via SpyTag/SpyCatcher spontaneous reaction.

    abstract::We generated a bifunctional enzyme chimera containing the xylanase and lichenase coupled with SpyTag between them. Meanwhile, we generated another chimera containing SpyCatcher and elastin-like polypeptides (ELPs). As ELPs could bond to the xylanase-lichenase chimera through SpyTag/SpyCatcher spontaneous reaction in m...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Lin Y,Jin W,Wang J,Cai Z,Wu S,Zhang G

    更新日期:2018-08-01 00:00:00

  • Optimization and modelling of enzymatic polymerization of ε-caprolactone to polycaprolactone using Candida Antartica Lipase B with response surface methodology and artificial neural network.

    abstract::Recently enzymatic catalysts have replaced organic and organometallic catalysts in the synthesis of bio-resorbable polymers. Enzymatic polymerization is considered as an alternative to conventional polymerization as they are less toxic, environmental friendly and can operate under mild conditions. In this research, th...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Pakalapati H,Tariq MA,Arumugasamy SK

    更新日期:2019-03-01 00:00:00

  • Glucosylation of the flavonoid, astragalin by Leuconostoc mesenteroides B-512FMCM dextransucrase acceptor reactions and characterization of the products.

    abstract::Astragalin (kaempferol-3-O-β-D-glucopyranoside, Ast) glucosides were synthesized by the acceptor reaction of a dextransucrase produced by Leuconostoc mesenteroides B-512FMCM with astragalin and sucrose. Each glucoside was purified and their structures were assigned as kaempferol-3-O-β-D-glucopyranosyl-(1→3)-O-α-D-gluc...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Kim GE,Kang HK,Seo ES,Jung SH,Park JS,Kim DH,Kim DW,Ahn SA,Sunwoo C,Kim D

    更新日期:2012-01-05 00:00:00

  • Covalent immobilization of a flavoprotein monooxygenase via its flavin cofactor.

    abstract::A generic approach for flavoenzyme immobilization was developed in which the flavin cofactor is used for anchoring enzymes onto the carrier. It exploits the tight binding of flavin cofactors to their target apo proteins. The method was tested for phenylacetone monooxygenase (PAMO) which is a well-studied and industria...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Krzek M,van Beek HL,Permentier HP,Bischoff R,Fraaije MW

    更新日期:2016-01-01 00:00:00

  • Characterization and solvent engineering of wheat β-amylase for enhancing its activity and stability.

    abstract::The kinetic and thermodynamic parameters of wheat β-amylase (WBA) were characterized and various additives were evaluated for enhancing its activity and thermostability. WBA activity was examined by neocuproine method using soluble starch as substrate. The Michaelis constant (K(m)) and molecular activity (k(cat)) were...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Daba T,Kojima K,Inouye K

    更新日期:2012-10-10 00:00:00

  • A new bi-modular endo-β-1,4-xylanase KRICT PX-3 from whole genome sequence of Paenibacillus terrae HPL-003.

    abstract::A new bi-modular, wide pH spectrum and highly active xylanase KRICT PX3 (JF320814) isolated from Paenibacillus terrae HPL-003 (KCTC11987BP) has been cloned and expressed in Escherichia coli. Purified recombinant xylanase KRICT PX-3 (1,620 bp, 540aa, NCBI accession number JF320814) showed highly active at 55°C in pH 4....

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Song HY,Lim HK,Kim DR,Lee KI,Hwang IT

    更新日期:2014-01-10 00:00:00

  • Effect of intense pulsed light on the deactivation of lipase: Enzyme-deactivation kinetics and tertiary structural changes by fragmentation.

    abstract::The effect of intense pulsed light (IPL) irradiation on Chromobacterium viscosum lipase was investigated with a primary focus on catalytic activity and molecular structure. During IPL irradiation, lipase activity decreased significantly with increasing pulse fluence (Fp) and exposure time (te). IPL-induced deactivatio...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Jeon MS,Park KM,Yu H,Park JY,Chang PS

    更新日期:2019-05-01 00:00:00

  • Development of Escherichia coli MG1655 strains to produce long chain fatty acids by engineering fatty acid synthesis (FAS) metabolism.

    abstract::The goal of this research was to develop recombinant Escherichia coli to improve fatty acid synthesis (FAS). Genes encoding acetyl-CoA carboxylase (accA, accB, accC), malonyl-CoA-[acyl-carrier-protein] transacylase (fabD), and acyl-acyl carrier protein thioesterase (EC gene), which are all enzymes that cataly...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Jeon E,Lee S,Won JI,Han SO,Kim J,Lee J

    更新日期:2011-06-10 00:00:00

  • Characterization of specificity of subtilisin Carlsberg towards peptide T by high-performance liquid chromatography and electrospray mass spectrometry.

    abstract::Peptide T has a sequence (Ala-Ser-Thr-Thr-Thr-Asn-Tyr-Thr) belonging to HIV envelope that is involved in the interaction with CD(4) receptor of T lymphocytes. Research of protease activities towards this peptide is very relevant for AIDS therapy. Characterization of specificity of subtilisin Carlsberg towards this ver...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Nedjar-Arroumea N,Paon M,Koralewski F,Friboulet A,Kouach M,Briand G,Leroy Y,Guillochon D

    更新日期:2000-03-01 00:00:00

  • Eco-friendly intracellular biosynthesis of CdS quantum dots without changing Escherichia coli's antibiotic resistance.

    abstract::In the paper, a green and efficient biosynthetical technique was reported for preparing cadmium sulfide (CdS) quantum dots, in which Escherichia coli (E. coli) was chosen as a biomatrix. Fluorescence emission spectra and fluorescent microscopic photographs revealed that as-produced CdS quantum dots had an optimum fluo...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Yan ZY,Du QQ,Qian J,Wan DY,Wu SM

    更新日期:2017-01-01 00:00:00

  • Purification and characterization of an extracellular alpha-amylase produced by Lactobacillus manihotivorans LMG 18010(T), an amylolytic lactic acid bacterium.

    abstract::This work presents the purification and characterization of an extracellular alpha-amylase (1,4-alpha-D-glucan glucanohydrolase, EC produced by a new lactic acid bacterium: Lactobacillus manihotivorans able to produce L(+) lactic acid from starch. The molecular weight was found to be 135 kDa. The temperature ...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Aguilar G,Morlon-Guyot J,Trejo-Aguilar B,Guyot JP

    更新日期:2000-09-01 00:00:00

  • DNase activity of micrococcal endonuclease covalently immobilized on nylon and polystyrene.

    abstract::Water-insoluble nucleases were prepared by immobilizing the endonuclease from S. aureus onto the surface of nylon-66 and polystyrene spheres. The activation phase of the synthetic supports was optimized to define optimal conditions of pH, temperature, and Ca2+ concentration for using immobilized enzymes. The activity,...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Michalon P,Roche J,Couturier R,Favre-Bonvin G,Marion C

    更新日期:1993-03-01 00:00:00

  • Penicillin amidase-catalysed transfer of low specific acyl moiety. Synthesis of 7-benzoxazolonylacetamido desacetoxycephalosporanic acid.

    abstract::The methyl ester of 2-benzoxazolon-3-yl-acetic acid was used as an acyl donor in the penicillin amidase-catalysed transfer reaction to 7-aminodesacetoxycephalosporanic acid. The synthesis of 7-(2-benzoxazolon-3-yl-acetamido)-desacetoxycephalosporanic acid was carried out as a kinetically controlled reaction. A charact...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Stambolieva N,Mincheva Z,Galunsky B,Kalcheva V

    更新日期:1992-06-01 00:00:00

  • γ-Glutamyl transpeptidase from Bacillus pumilus KS 12: decoupling autoprocessing from catalysis and molecular characterization of N-terminal region.

    abstract::Gamma glutamyl transpeptidase from Bacillus pumilus KS12 (GGTBP) was cloned, expressed in pET-28-E. coli expression system as a heterodimeric enzyme with molecular weights of 45 and 20 kDa for large and small subunit, respectively. It was purified by nickel affinity chromatography with hydrolytic and transpeptidase ac...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Murty NA,Tiwary E,Sharma R,Nair N,Gupta R

    更新日期:2012-03-10 00:00:00

  • Evaluation and modelling the utility of SCCO2 to support efficient lipase mediated esterification.

    abstract::Supercritical fluids offer environmental advantages over chemical solvents, while providing enhanced separation and chemical selectivity. The use of supercritical fluids for the recovery of products from biomass and the transformation of selected molecules (to add value) was studied. Free fatty acids were bio-catalyti...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Baig MN,Santos RC,Zetzl C,King J,Pioch D,Bowra S

    更新日期:2011-09-10 00:00:00

  • Screening and identification of five peptides from pinto bean with inhibitory activities against α-amylase using phage display technique.

    abstract::The objective of this study was to screen and identify α-amylase inhibitor peptides from Pinto bean. Five Pinto bean bioactive peptides were successfully identified: PPHMLP (P1), PLPWGAGF (P3), PPHMGGP (P6), PLPLHMLP (P7) and LSSLEMGSLGALFVCM (P9). Based on ELISA results, their promising optical density values were 1....

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Ngoh YY,Lim TS,Gan CY

    更新日期:2016-07-01 00:00:00

  • Multiple lignin peroxidases of Phanerochaete chrysosporium INA-12.

    abstract::Nine proteins with lignin peroxidase activity were separated from cultures of Phanerochaete chrysosporium INA-12 in glycerol as carbon source and non-nitrogen limited. Four lignin peroxidase isozymes (4, 5, 8, 9) were purified and characterized. Although differences in kinetic parameters could be shown, antibody react...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Odier E,Delattre M

    更新日期:1990-06-01 00:00:00

  • Studies on the purification of peroxidase from horseradish roots using reverse micelles.

    abstract::Horseradish peroxidase (HRP) was successfully purified from horseradish roots by a two-stage reverse-micellar extraction from the dialyzed aqueous extract. The anionic surfactant AOT dissolved in isooctane was used to produce the reverse-micellar phases. The narrow pH range at which HRP solubilization occurred was exp...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Regalado C,Asenjo JA,Pyle DL

    更新日期:1996-04-01 00:00:00

  • Utilization of distillery wastewater for hydrogen production in one-stage and two-stage processes involving photofermentation.

    abstract::In this study, distillery wastewater was treated by dark fermentation or photofermentation alone, and by sequential dark and photofermentation processes using anaerobic saccharolytic consortium and purple nonsulfur bacteria. Combination of dark and photofermentation resulted in the maximal H2 yield of 17.6L/L of disti...

    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Laurinavichene T,Tekucheva D,Laurinavichius K,Tsygankov A

    更新日期:2018-03-01 00:00:00