Abstract:
:2,5-Bis(hydroxymethyl)furan (BHMF) is a versatile building block in the synthesis of polymers, fuels, and macrocycle polyethers. In this work, alcohol dehydrogenases (ADHs) were identified from Meyerozyma guilliermondii SC1103 and were heterologously expressed in Saccharomyces cerevisiae for the synthesis of BHMF from 5-hydroxymethylfurfural (HMF). Of recombinant strains constructed, S. cerevisiae expressing an aryl ADH (MgAAD1669) was observed to be the best catalyst. Upon process optimization, BHMF was afforded with a 99% selectivity and a 94% yield within 24 h at the substrate concentration of 250 mM. The space-time yield up to 3.4 g/L h was achieved in the fed-batch synthesis of BHMF. Inexpensive corncob hydrolysate proved to be a promising alternative to glucose as co-substrate for biocatalytic synthesis of BHMF, thus resulting in the significantly reduced production cost.
journal_name
Enzyme Microb Technoljournal_title
Enzyme and microbial technologyauthors
Xia ZH,Zong MH,Li Ndoi
10.1016/j.enzmictec.2019.109491subject
Has Abstractpub_date
2020-03-01 00:00:00pages
109491eissn
0141-0229issn
1879-0909pii
S0141-0229(19)30229-7journal_volume
134pub_type
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