ZnS quantum dots as pH probes for study of enzyme reaction kinetics.

abstract：:Direct cellobiose production from cellulose by a genetically modified fungus-Neurospora crassa, was explored in this study. A library of N. crassa sextuple beta-glucosidase (bgl) gene deletion strains was constructed. Various concentrations of cellobiose were detected in the culture broth of the N. crassa sextuple bet...

journal_title：Enzyme and microbial technology

authors： Wu W,Hildebrand A,Kasuga T,Xiong X,Fan Z

更新日期：2013-03-05 00:00:00

abstract：:During toluene hydroxylation, catalyzed by Pseudomonas putida UV4 one molecule of oxygen is added to the aromatic ring to produce the dihydroxylated (non-aromatic) ring structure, toluene cis-glycol. Toluene, which is toxic to the cells at aqueous phase concentration above ( approximately 2.4 mmol), is fed to the reac...

journal_title：Enzyme and microbial technology

authors： Carragher JM,McClean WS,Woodley JM,Hack CJ

更新日期：2001-02-01 00:00:00

abstract：:We demonstrate the feasibility of growing crystals of protein in volumes as small as 1 nanoliter. Advances in the handling of very small volumes (i.e. through inkjet and other technologies) open the way towards fully automated systems. The rationale for these experiments is the desire to develop a system that speeds u...

journal_title：Enzyme and microbial technology

authors： Kuil ME,Bodenstaff ER,Hoedemaeker FJ,Abrahams JP

更新日期：2002-03-13 00:00:00

abstract：:A new bi-modular, wide pH spectrum and highly active xylanase KRICT PX3 (JF320814) isolated from Paenibacillus terrae HPL-003 (KCTC11987BP) has been cloned and expressed in Escherichia coli. Purified recombinant xylanase KRICT PX-3 (1,620 bp, 540aa, NCBI accession number JF320814) showed highly active at 55°C in pH 4....

journal_title：Enzyme and microbial technology

authors： Song HY,Lim HK,Kim DR,Lee KI,Hwang IT

更新日期：2014-01-10 00:00:00

abstract：:Numerous studies have focused on how to obtain high yield of sophorolipids using low-cost materials as substrates, and there has been various work on the experimental methods for purifying lactonic sophorolipids. These studies have not yet obtained satisfied results in combining a low-cost fermentation process and the...

journal_title：Enzyme and microbial technology

authors： Yang X,Zhu L,Xue C,Chen Y,Qu L,Lu W

更新日期：2012-12-10 00:00:00

abstract：:Enzymatic synthesis of cephalothin from 7-aminocephalosporanic acid (7-ACA) and amide derivatives of 2-thienylacetic acid (2-TA) using penicillin G acylase (pen G acylase) was studied. Two amide derivatives of 2-TA namely 2-thienylacetamide (2-TAA) and 2-thienylacetohydroxamic acid (2-TAH) were used in this study. The...

journal_title：Enzyme and microbial technology

authors： Shaw S,Shyu J,Hsieh Y,Yeh H

更新日期：2000-02-01 00:00:00

abstract：:Solid-liquid enzyme reactions constitute important processes in biochemical industries. The isomerization of d-glucose to d-fructose, using the immobilized glucose isomerase (Sweetzyme T), as a typical example of solid-liquid catalyzed reactions has been carried out in one stage and multi-stage novel type of impinging...

journal_title：Enzyme and microbial technology

authors： Fatourehchi N,Sohrabi M,Dabir B,Royaee SJ,Haji Malayeri A

更新日期：2014-02-05 00:00:00

abstract：:A method to quantify the impact of proteolysis on accumulation of recombinant proteins in E. coli is described. A much smaller intracellular concentration of staphylococcal protein A (SpA) (14.7 mg. g(-1)) compared to the fusion protein SpA-betagalactosidase (138 mg. g(-1)) is explained by a very high proteolysis rate...

journal_title：Enzyme and microbial technology

authors： Rozkov A,Schweder T,Veide A,Enfors S

更新日期：2000-12-01 00:00:00

abstract：:Before applying nanotechnologies in biomedical and environmental areas it is advised to study interactions of nanoparticles and other nanomaterials with biomacromolecule present in living system. Moreover there is scarcity of reports on interactions between nanoparticles and biomaterials. In present report a rapid, ec...

journal_title：Enzyme and microbial technology

authors： Patil CD,Borase HP,Suryawanshi RK,Patil SV

更新日期：2016-10-01 00:00:00

abstract：:The hydrolytic activity of a thermophilic cyclomaltodextrinase (CMD) from Anoxybacillus flavithermus ZNU-NGA and a representative single mutant were investigated against soluble substrates including α-, β- and γ-cyclomaltodestrines (CDs). Based on the occurrence of arginine (Arg) at position 403 in some homologue prot...

journal_title：Enzyme and microbial technology

authors： Mirzaee Z,Jafarian V,Shirdel SA,Khalifeh K

更新日期：2019-12-01 00:00:00

abstract：:Addition of non-ionic surfactants (NIS) is known to accelerate enzymatic lignocellulose hydrolysis. The mechanism behind this accelerating effect is still not elucidated but has been hypothesized to originate from favorable NIS-lignin interactions which alleviate non-productive adsorption of cellulases to lignin. In t...

journal_title：Enzyme and microbial technology

authors： Olsen SN,Bohlin C,Murphy L,Borch K,McFarland KC,Sweeny MD,Westh P

更新日期：2011-09-10 00:00:00

abstract：:3-Mercaptopyruvate (3MPy), a structural analog of 3-mercaptopropionic acid, is a precursor compound for biosynthesis of polythioesters in bacteria. The cost-effectiveness and sustainability of the whole process could be greatly improved by using the cysteine degradation pathway for an intracellular supply of 3MPy. Tra...

journal_title：Enzyme and microbial technology

authors： Andreeßen C,Gerlt V,Steinbüchel A

更新日期：2017-04-01 00:00:00

abstract：:The possibility of using thermostable inulinases from Aspergillus ficuum in place of invertase for sucrose hydrolysis was explored. The commercial inulinases preparation was immobilized onto porous glass beads by covalent coupling using activation by a silane reagent and glutaraldehyde before adding the enzyme. The im...

journal_title：Enzyme and microbial technology

authors： Ettalibi M,Baratti JC

更新日期：2001-05-07 00:00:00

abstract：:When dissolved oxygen (DO) was maintained at saturation level during batch fermentations of Streptomyces clavuligerus (NRRL 3585), the accumulation of the intermediate penicillin N was lowered while formation of the end product cephamycin C was increased relative to fermentations without DO control. The specific activ...

journal_title：Enzyme and microbial technology

authors： Rollins MJ,Jensen SE,Wolfe S,Westlake DW

更新日期：1990-01-01 00:00:00

abstract：:Phenylalanine ammonia-lyase (PAL) gene plays an important role in the synthesis of flavones, lignin, and other bioactive compounds in living organisms. Inonotus baumii, the only known flavone-producing filamentous fungus, is of great importance in the investigation of flavone metabolic pathways. To study the function ...

journal_title：Enzyme and microbial technology

authors： Lin W,Liu A,Weng C,Li H,Sun S,Song A,Zhu H

更新日期：2018-05-01 00:00:00

abstract：:The stabilization of fungal cellulases by spray drying, the thermal stability of Penicillium occitanis cellulases and the effect of some additives were studied. We observed that CMCase activity presents a good stability at 50 degrees C, even after 60 h of incubation. On the other hand, beta-glucosidase activity was mo...

journal_title：Enzyme and microbial technology

authors： Belghith H,Ellouz Chaabouni S,Gargouri A

更新日期：2001-02-01 00:00:00

abstract：:Biosensors have been extensively studied during the last 20 years, and a myriad of laboratory biosensors have been developed. Improvements are required in biosensor design and performance before they become widely accepted in industrial process monitoring. However, as the biotechnology industry expands, biosensors may...

journal_title：Enzyme and microbial technology

authors： Brooks SL,Higgins IJ,Newman JD,Turner AP

更新日期：1991-12-01 00:00:00

abstract：:A solvent-mediated method (SMM) was used to prepare supersaturated sugar solutions in hydrophobic and mixture of hydrophilic/hydrophobic ionic liquids (ILs), namely, [Bmim][Tf2N] and [Bmim][TfO]/[Bmim][Tf2N], respectively. In this method, sugars were first solubilized in a mixture of organic solvent and water (i.e. me...

journal_title：Enzyme and microbial technology

authors： Shin DW,Mai NL,Bae SW,Koo YM

更新日期：2019-07-01 00:00:00

abstract：:Detection of mRNA is a valuable method for monitoring the specific gene expression. In this study, we devised a novel cDNA synthesis method using three enzymes, the genetically engineered thermostable variant of reverse transcriptase (RT), MM4 (E286R/E302K/L435R/D524A) from Moloney murine leukemia virus (MMLV), the ge...

journal_title：Enzyme and microbial technology

authors： Okano H,Katano Y,Baba M,Fujiwara A,Hidese R,Fujiwara S,Yanagihara I,Hayashi T,Kojima K,Takita T,Yasukawa K

更新日期：2017-01-01 00:00:00

abstract：:The kinetics of erythromycin production were dependent on the identity of the growth rate-limiting nutrient during batch cultures of Saccharopolyspora erythraea. Semilogarithmic linear regression provided a single estimate of growth rate during the exponential phase, but partial cubic spline curve fit derivatives prov...

journal_title：Enzyme and microbial technology

authors： McDermott JF,Lethbridge G,Bushell ME

更新日期：1993-08-01 00:00:00

abstract：:l-asparaginase is a pharmaceutically and industrially important enzyme as it has potential to treat different cancers and inhibit acrylamide formation in fried and baked food products. In the present study, an attempt was made to screen for new and novel l-asparaginase producers using a widely applied phenol red and b...

journal_title：Enzyme and microbial technology

authors： Mihooliya KN,Nandal J,Swami L,Verma H,Chopra L,Sahoo DK

更新日期：2017-12-01 00:00:00

abstract：:The fatty acid methyl esters and fatty acid ethyl esters are known as biodiesels which are considered to be renewable, nontoxic and biodegradable biofuels. However, the conventional biodiesels show a high crystallization temperature which is one of the most critical obstacles against the widespread biodiesel usage. Th...

journal_title：Enzyme and microbial technology

authors： Pan H,Zhang L,Li X,Guo D

更新日期：2017-07-01 00:00:00

abstract：:Peptide T has a sequence (Ala-Ser-Thr-Thr-Thr-Asn-Tyr-Thr) belonging to HIV envelope that is involved in the interaction with CD(4) receptor of T lymphocytes. Research of protease activities towards this peptide is very relevant for AIDS therapy. Characterization of specificity of subtilisin Carlsberg towards this ver...

journal_title：Enzyme and microbial technology

authors： Nedjar-Arroumea N,Paon M,Koralewski F,Friboulet A,Kouach M,Briand G,Leroy Y,Guillochon D

更新日期：2000-03-01 00:00:00

abstract：:'Candidatus Liberibacter asiaticus' ('Ca. L. asiaticus'), the suspected causative agent of citrus greening disease, is one of many phloem-restricted plant pathogens that have not been isolated and grown in an axenic culture. In this study, infected Asian citrus psyllids were used to prepare a host-free source of 'Ca. ...

journal_title：Enzyme and microbial technology

authors： Molki B,Call DR,Ha PT,Omsland A,Gang DR,Lindemann SR,Killiny N,Beyenal H

更新日期：2020-12-01 00:00:00

abstract：:The cellulase and xylanase genes of filamentous Trichoderma fungi exist under carbon catabolite repression mediated by the regulator carbon catabolite repressor (CREI). Our objective was to find the role of CREI in a cellulase-hyperproducing mutant of Trichoderma koningii, and address whether enzyme production can be ...

journal_title：Enzyme and microbial technology

authors： Wang S,Liu G,Yu J,Tian S,Huang B,Xing M

更新日期：2013-07-10 00:00:00

abstract：:A novel automated image analysis method is described for characterizing the viability and morphology of animal cells from suspension cultures. With the aid of the exclusion dye Trypan Blue, the total and viable cell counts and the percentage of dead cells present are found. The area, perimeter, equivalent diameter, an...

journal_title：Enzyme and microbial technology

authors： Tucker KG,Chalder S,al-Rubeai M,Thomas CR

更新日期：1994-01-01 00:00:00

abstract：:NADPH is the key cofactor in L-isoleucine (Ile) biosynthetic pathway. To increase the Ile biosynthesis in Corynebacterium glutamicum ssp. lactofermentum JHI3-156, NADPH supply needs to be enhanced. Here NAD kinase, the key enzyme for the de novo biosynthesis of NADP(+) and NADPH, were cloned and expressed in JHI3-156,...

journal_title：Enzyme and microbial technology

authors： Shi F,Huan X,Wang X,Ning J

更新日期：2012-07-15 00:00:00

abstract：:Acetobacter pasteurianus oxidizes glycidol with high activity, comparable to the oxidation of ethanol. The organism has a preference for the S-enantiomer, and the kinetic resolution process obeys a simple relationship, indicating an enantiomeric ratio (E) of 19. The compound is converted into glycidic acid, although a...

journal_title：Enzyme and microbial technology

authors： Geerlof A,Jongejan JA,van Dooren TJ,Racemakers-Franken PC,van den Tweel WJ,Duine JA

更新日期：1994-12-01 00:00:00

abstract：:Resveratrol is a well-known polyphenol present in red wine and exerts antioxidative and anti-carcinogenic effects on the human body. To produce resveratrol in a food-grade yeast, the 4-coumarate:coenzyme A ligase gene (4CL1) from Arabidopsis thaliana and stilbene synthase gene (STS) from Arachis hypogaea were cloned a...

journal_title：Enzyme and microbial technology

authors： Shin SY,Han NS,Park YC,Kim MD,Seo JH

更新日期：2011-01-05 00:00:00

abstract：:The effect of intense pulsed light (IPL) irradiation on Chromobacterium viscosum lipase was investigated with a primary focus on catalytic activity and molecular structure. During IPL irradiation, lipase activity decreased significantly with increasing pulse fluence (Fp) and exposure time (te). IPL-induced deactivatio...

journal_title：Enzyme and microbial technology

authors： Jeon MS,Park KM,Yu H,Park JY,Chang PS

更新日期：2019-05-01 00:00:00