当前位置： SCI文献检索 > ENZYME AND MICROBIAL TECHNOLOGY期刊下所有文献 > Factors relevant to the production of (R)-(+)-glycidol (2,3-epoxy-1-propanol) from racemic glycidol by enantioselective oxidation with Acetobacter pasteurianus ATCC 12874.

Factors relevant to the production of (R)-(+)-glycidol (2,3-epoxy-1-propanol) from racemic glycidol by enantioselective oxidation with Acetobacter pasteurianus ATCC 12874.

Abstract：

:Acetobacter pasteurianus oxidizes glycidol with high activity, comparable to the oxidation of ethanol. The organism has a preference for the S-enantiomer, and the kinetic resolution process obeys a simple relationship, indicating an enantiomeric ratio (E) of 19. The compound is converted into glycidic acid, although a transient accumulation of glycidaldehyde occurs initially. Determination of other parameters revealed a temperature optimum of 50 degrees C, long-term stability (cells in the resting state), and a pH optimum compatible with the chemical stability of glycidol. However, it was also noted that respiration rates decrease at concentrations of glycidol above 1 M. This is most likely caused by substrate inhibition of the glycidol-oxidizing enzyme, the quinohemoprotein ethanol dehydrogenase. Comparison with existing methods for enantiomerically pure glycidol production indicated a number of attractive points for the method described here, although definitive evaluation must await further studies on the long-term stability under process conditions, reusability of the cells, and the mechanism of glycidol inhibition.

journal_name

Enzyme Microb Technol

journal_title

Enzyme and microbial technology

authors

Geerlof A,Jongejan JA,van Dooren TJ,Racemakers-Franken PC,van den Tweel WJ,Duine JA

doi

10.1016/0141-0229(94)90143-0

subject

Has Abstract

pub_date

1994-12-01 00:00:00

pages

1059-63

issue

12

eissn

0141-0229

issn

1879-0909

pii

0141-0229(94)90143-0

journal_volume

16

pub_type

杂志文章

相关文献

ENZYME AND MICROBIAL TECHNOLOGY文献大全
  • Alginate as immobilization matrix and stabilizing agent in a two-phase liquid system: application in lipase-catalysed reactions.

    abstract：:Alginate was evaluated as an immobilization matrix for enzyme-catalyzed reactions in organic solvents. In contrast to most hydrogels, calcium alginate was found to be stable in a range of organic solvents and to retain the enzyme inside the gel matrix. In hydrophobic solvents, the alginate gel (greater than 95% water)...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/0141-0229(92)90024-i

    authors： Hertzberg S,Kvittingen L,Anthonsen T,Skjåk-Braek G

    更新日期：1992-01-01 00:00:00

  • Overexpression of hlyB and mdh genes confers halotolerance in Fremyella diplosiphon, a freshwater cyanobacterium.

    abstract：:Fremyella diplosiphon is a freshwater cyanobacterium that has great potential as a biofuel agent due to its ability to grow in low light intensity and acclimation to different wavelengths. To enhance its halotolerance for growth in 35gL-1 sodium chloride (NaCl), plasmids harboring hemolysin B (hlyB) and malate dehydro...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2017.04.009

    authors： Tabatabai B,Arumanayagam AS,Enitan O,Mani A,Natarajan SS,Sitther V

    更新日期：2017-08-01 00:00:00

  • Carbohydrate-binding property of a cell wall integrity and stress response component (WSC) domain of an alcohol oxidase from the rice blast pathogen Pyricularia oryzae.

    abstract：:The cell wall integrity and stress response component (WSC) domain was first described in the Wsc-family protein of the yeast Saccharomyces cerevisiae, and later found in diverse eukaryotic organisms. Due solely to their presence in the Wsc-family proteins working as a plasma membrane sensor for surface stress and in ...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2019.02.009

    authors： Oide S,Tanaka Y,Watanabe A,Inui M

    更新日期：2019-06-01 00:00:00

  • Optimization of the growth conditions of Sf21 insect cells for high-density perfusion culture in stirred-tank bioreactors.

    abstract：:Spodoptera frugiperda insect cells (IPLB-Sf21-AE) (Sf21), infected with baculovirus expression vectors during their exponential growth phase, are commonly used to produce a variety of heterologous recombinant proteins. In the present study the culture conditions of these insect cells were studied to establish high-den...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/0141-0229(94)90022-1

    authors： Deutschmann SM,Jäger V

    更新日期：1994-06-01 00:00:00

  • The potential of positively-charged cellulose sponge for malolactic fermentation of wine, using Oenococcus oeni.

    abstract：:Malolactic fermentation (MLF) is a secondary bioconversion developed in some wines involving malic acid decarboxylation. The induction of MLF in wine by cultures of free and immobilized Oenococcus oeni cells was investigated. This work reports on the effect of surface charges in the immobilization material, a recently...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/s0141-0229(00)00339-2

    authors： Maicas S,Pardo I,Ferrer S

    更新日期：2001-03-08 00:00:00

  • New-to-nature sophorose analog: a potent inducer for gene expression in Trichoderma reesei.

    abstract：:Controlled hydrolysis of lactonic sophorolipids from Starmerella bombicola yields a previously undescribed sophorose analog that potently induces cellulase in Trichoderma reesei Rut-C30. Acid treatment of natural sophorolipids results in a mixture of monoacetylated, deacetylated, and diacetylated sophorolipids in acid...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2016.01.003

    authors： Huang TT,Wages JM

    更新日期：2016-04-01 00:00:00

  • Increasing the refolding efficiency in vitro by site-directed mutagenesis of Cys383 in rat procarboxypeptidase B.

    abstract：:This study examines a novel method to reduce the probability of disulfide mismatches during the refolding process by the replacement of cysteines within a protein. Specifically, Cys383 of recombinant rat procarboxypeptidase B was replaced by other amino acids to increase the refolding efficiency in vitro. Mutants C383...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2011.04.018

    authors： Li S,Zhang L,Wu Q,Xin A,Zhao J,Fan L

    更新日期：2011-07-10 00:00:00

  • Extracellular biosynthesis of copper sulfide nanoparticles by Shewanella oneidensis MR-1 as a photothermal agent.

    abstract：:Photothermal therapy (PTT) is a minimally invasive and effective cancer treatment method and has a great potential for innovating the conventional chemotherapy approaches. Copper sulfide (CuS) exhibits photostability, low cost, and high absorption in near infrared region, and is recognized as an ideal candidate for PT...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2016.04.002

    authors： Zhou NQ,Tian LJ,Wang YC,Li DB,Li PP,Zhang X,Yu HQ

    更新日期：2016-12-01 00:00:00

  • Construction of glutamate biosensor based on covalent immobilization of glutamate oxidase on polypyrrole nanoparticles/polyaniline modified gold electrode.

    abstract：:A method is described for construction of a highly sensitive electrochemical biosensor for detection of glutamate. The biosensor is based on covalent immobilization of glutamate oxidase (GluOx) onto polypyrrole nanoparticles and polyaniline composite film (PPyNPs/PANI) electrodeposited onto Au electrode. The enzyme el...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2014.02.001

    authors： Batra B,Kumari S,Pundir CS

    更新日期：2014-04-10 00:00:00

  • Phenol-oxidase (laccase) activity in strains of the hyphomycete Chalara paradoxa isolated from olive mill wastewater disposal ponds.

    abstract：:Production of laccase activity by nine strains of Chalara paradoxa isolated from olive mill wastewater disposal ponds were studied. Enzyme extracts obtained from cultured broths by adsorption on hydroxyapatite showed a single band of laccase activity on ABTS after polyacrylamide gel electrophoresis (PAGE). They showed...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/s0141-0229(99)00197-0

    authors： Robles A,Lucas R,de Cienfuegos GA,Gálvez A

    更新日期：2000-04-01 00:00:00

  • Effect of intense pulsed light on the deactivation of lipase: Enzyme-deactivation kinetics and tertiary structural changes by fragmentation.

    abstract：:The effect of intense pulsed light (IPL) irradiation on Chromobacterium viscosum lipase was investigated with a primary focus on catalytic activity and molecular structure. During IPL irradiation, lipase activity decreased significantly with increasing pulse fluence (Fp) and exposure time (te). IPL-induced deactivatio...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2019.02.001

    authors： Jeon MS,Park KM,Yu H,Park JY,Chang PS

    更新日期：2019-05-01 00:00:00

  • Course of pH during the formation of amoxicillin by a suspension-to-suspension reaction.

    abstract：:Amoxicillin can be produced in an enzymatic suspension-to-suspension reaction in which the substrate(s) and product(s) are mainly present as solid particles, while the reaction takes place in the liquid phase. During these suspension-to-suspension reactions different subprocesses take place, such as dissolution/crysta...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/s0141-0229(00)00245-3

    authors： Diender MB,Straathof AJ,van der Does T,Zomerdijk M,Heijnen JJ

    更新日期：2000-11-01 00:00:00

  • Characterization of two acidic β-glucosidases and ethanol fermentation in the brown rot fungus Fomitopsis palustris.

    abstract：:Two acidic β-glucosidases (βGI and βGII) from the brown rot fungus Fomitopsis palustris were purified to homogeneity by several chromatographic steps. βGI and βGII had molecular weights of 130 and 213 kDa, respectively, and exhibited optimum activity at pH 2.5 and 55°C. The K(m) values of βGI and βGII for p-nitropheny...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2010.12.012

    authors： Okamoto K,Sugita Y,Nishikori N,Nitta Y,Yanase H

    更新日期：2011-04-07 00:00:00

  • Characterization of two truncated forms of xylanase recombinantly expressed by Lactobacillus reuteri with an introduced rumen fungal xylanase gene.

    abstract：:The xylanase R8 gene (xynR8) from uncultured rumen fungi was cloned and successfully expressed in Lactobacillus reuteri. A xylanase activity of 132.1 U/mL was found in the broth of L. reuteri R8, the transformant containing pNZ3004 vector with xynR8 gene insertion. Two distinct forms of recombinant xylanase with diffe...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2014.06.004

    authors： Cheng HL,Hu CY,Lin SH,Wang JY,Liu JR,Chen YC

    更新日期：2014-10-01 00:00:00

  • A biosensor based on gold nanoparticles, dihexadecylphosphate, and tyrosinase for the determination of catechol in natural water.

    abstract：:In this work, a biosensor using a glassy carbon electrode modified with gold nanoparticles (AuNPs) and tyrosinase (Tyr) within a dihexadecylphosphate film is proposed. Cystamine and glutaraldehyde crosslinking agents were used as a support for Tyr immobilization. The proposed biosensor was characterized by scanning el...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2015.12.004

    authors： Campanhã Vicentini F,Garcia LL,Figueiredo-Filho LC,Janegitz BC,Fatibello-Filho O

    更新日期：2016-03-01 00:00:00

  • Engineering the central pathways in Lactococcus lactis: functional expression of the phosphofructokinase (pfk) and alternative oxidase (aox1) genes from Aspergillus niger in Lactococcus lactis facilitates improved carbon conversion rates under oxidizing c

    abstract：:The present work describes a novel central pathway engineering method that has been designed with the aim to increase the carbon conversion rates under oxidizing conditions in L. lactis fermentations. The nisin producer L. lactis ATCC11454 strain has been genetically engineered by cloning a truncated version of the ph...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2012.04.007

    authors： Papagianni M,Avramidis N

    更新日期：2012-08-10 00:00:00

  • Novel glycosylated human interferon alpha 2b expressed in glycoengineered Pichia pastoris and its biological activity: N-linked glycoengineering approach.

    abstract：:Human interferon alpha 2b (IFN α2b) is a type I interferon exhibiting antiviral, anti-proliferative and immunomodulatory activities. The clinical outcome of the approved recombinant human IFN α2b drugs in the market suffers from short plasma half-life, rapid clearance and other side effects. Human IFN α2b expression i...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2019.05.007

    authors： Katla S,Yoganand KNR,Hingane S,Ranjith Kumar CT,Anand B,Sivaprakasam S

    更新日期：2019-09-01 00:00:00

  • Flow cytometric immunoassay using E. coli with autodisplayed Z-domains.

    abstract：:Recently, we reported a highly sensitive immunoassay using Escherichia coli cells with autodisplayed Z-domains. In this work, E. coli cells with autodisplayed Z-domains were applied to the flow-cytometry-based simultaneous detection of multiple analytes. The E. coli cells were doubly transfected to express a fluoresce...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2013.03.018

    authors： Park M,Bong JH,Yoo G,Jose J,Kang MJ,Pyun JC

    更新日期：2013-08-15 00:00:00

  • Synthesis of long-chain alkyl glucosides via reverse hydrolysis reactions catalyzed by an engineered β-glucosidase.

    abstract：:Long-chain alkyl glucosides, such as octyl and decyl β-d-glucopyranosides (OG and DG, respectively), are regarded as a new generation of biodegradable, non-ionic surfactants. Previously, the mutants of Dalbergia cochinchinensis Pierre dalcochinase showed potential in the synthesis of oligosaccharides and alkyl glucosi...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2020.109591

    authors： Thenchartanan P,Pitchayatanakorn P,Wattana-Amorn P,Ardá A,Svasti J,Jiménez-Barbero J,Kongsaeree PT

    更新日期：2020-10-01 00:00:00

  • Effects of non-ionic surfactants on the interactions between cellulases and tannic acid: a model system for cellulase-poly-phenol interactions.

    abstract：:Addition of non-ionic surfactants (NIS) is known to accelerate enzymatic lignocellulose hydrolysis. The mechanism behind this accelerating effect is still not elucidated but has been hypothesized to originate from favorable NIS-lignin interactions which alleviate non-productive adsorption of cellulases to lignin. In t...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2011.06.015

    authors： Olsen SN,Bohlin C,Murphy L,Borch K,McFarland KC,Sweeny MD,Westh P

    更新日期：2011-09-10 00:00:00

  • Enhanced detection of RNA by MMLV reverse transcriptase coupled with thermostable DNA polymerase and DNA/RNA helicase.

    abstract：:Detection of mRNA is a valuable method for monitoring the specific gene expression. In this study, we devised a novel cDNA synthesis method using three enzymes, the genetically engineered thermostable variant of reverse transcriptase (RT), MM4 (E286R/E302K/L435R/D524A) from Moloney murine leukemia virus (MMLV), the ge...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2016.10.003

    authors： Okano H,Katano Y,Baba M,Fujiwara A,Hidese R,Fujiwara S,Yanagihara I,Hayashi T,Kojima K,Takita T,Yasukawa K

    更新日期：2017-01-01 00:00:00

  • A new bi-modular endo-β-1,4-xylanase KRICT PX-3 from whole genome sequence of Paenibacillus terrae HPL-003.

    abstract：:A new bi-modular, wide pH spectrum and highly active xylanase KRICT PX3 (JF320814) isolated from Paenibacillus terrae HPL-003 (KCTC11987BP) has been cloned and expressed in Escherichia coli. Purified recombinant xylanase KRICT PX-3 (1,620 bp, 540aa, NCBI accession number JF320814) showed highly active at 55°C in pH 4....

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2013.09.002

    authors： Song HY,Lim HK,Kim DR,Lee KI,Hwang IT

    更新日期：2014-01-10 00:00:00

  • Electricity generation from carbon monoxide in a single chamber microbial fuel cell.

    abstract：:Electricity production from carbon monoxide (CO) is demonstrated in a single chamber microbial fuel cell (MFC) with a CoTMPP-based air cathode. The MFC was inoculated with anaerobic sludge and continuously sparged with CO as a sole carbon source. Volumetric power output was maximized at a CO flow rate of 4.8LLR(-1)d(-...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2010.02.010

    authors： Mehta P,Hussain A,Tartakovsky B,Neburchilov V,Raghavan V,Wang H,Guiot SR

    更新日期：2010-05-05 00:00:00

  • Improvement of catalytical properties of two invertases highly tolerant to sucrose after expression in Pichia pastoris. Effect of glycosylation on enzyme properties.

    abstract：:Zymomonas mobilis genes encoding INVA and INVB were expressed in Pichia pastoris, under the control of the strong AOX1 promoter, and the recombinant enzymes were named INVAAOX1 and INVBAOX1. The expression levels of INVAAOX1 (1660 U/mg) and INVBAOX1 (1993 U/mg) in P. pastoris were 9- and 7-fold higher than those obser...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2015.11.008

    authors： Pérez de los Santos AI,Cayetano-Cruz M,Gutiérrez-Antón M,Santiago-Hernández A,Plascencia-Espinosa M,Farrés A,Hidalgo-Lara ME

    更新日期：2016-02-01 00:00:00

  • On-line chemiluminescence assay using FIA and fiber optics for urinary and blood glucose.

    abstract：:A chemiluminescence fiber optic system coupled to flow injection analysis (FIA) and ion exchange chromatography has been developed for determining glucose in blood and urine. Immobilized glucose oxidase acted on beta-D-glucose to produce hydrogen peroxide, which was then reacted with luminol in the presence of ferricy...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/0141-0229(93)90130-t

    authors： Cattaneo MV,Luong JH

    更新日期：1993-05-01 00:00:00

  • Recovery of purified lactonic sophorolipids by spontaneous crystallization during the fermentation of sugarcane molasses with Candida albicans O-13-1.

    abstract：:Numerous studies have focused on how to obtain high yield of sophorolipids using low-cost materials as substrates, and there has been various work on the experimental methods for purifying lactonic sophorolipids. These studies have not yet obtained satisfied results in combining a low-cost fermentation process and the...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2012.08.002

    authors： Yang X,Zhu L,Xue C,Chen Y,Qu L,Lu W

    更新日期：2012-12-10 00:00:00

  • The influence of hexoses addition on the fermentation of d-xylose in Debaryomyces hansenii under continuous cultivation.

    abstract：:The effect of hexoses (glucose and galactose) addition to the feed xylose mineral medium of Debaryomyces hansenii chemostat cultures grown at a constant dilution rate of 0.055 h(-1) was studied. Xylitol was the major product detected amongst all tested conditions. The maximal values for xylitol yield and volumetric pr...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/s0141-0229(00)00166-6

    authors： Tavares JM,Duarte LC,Amaral-Collaço MT,Gírio FM

    更新日期：2000-06-01 00:00:00

  • From gene to product in yeast: production of fungal cutinase.

    abstract：:In the mid-1970s, information technology and recombinant DNA technology were considered as the breakthrough technologies of the final quarter of the 20th century. Now, about 25 years later, information technology has penetrated deeply into our society and nearly everyone uses this technology. Compared to the formidabl...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/s0141-0229(00)00176-9

    authors： Verripsab T,Duboc P,Visser C,Sagt C

    更新日期：2000-06-01 00:00:00

  • Cellulose oxidation and bleaching processes based on recombinant Myriococcum thermophilum cellobiose dehydrogenase.

    abstract：:Myriococcum thermophilum cellobiose dehydrogenase (MtCDH) was expressed in Pichia pastoris using the pPICZαA expression vector under the control of methanol inducible AOX promoter. The purified recombinant MtCDH with a specific activity of 3.1 Umg(-1) was characterized to obtain kinetic constants for various carbohydr...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/j.enzmictec.2012.10.007

    authors： Flitsch A,Prasetyo EN,Sygmund C,Ludwig R,Nyanhongo GS,Guebitz GM

    更新日期：2013-01-10 00:00:00

  • Sucrose hydrolysis by thermostable immobilized inulinases from aspergillus ficuum.

    abstract：:The possibility of using thermostable inulinases from Aspergillus ficuum in place of invertase for sucrose hydrolysis was explored. The commercial inulinases preparation was immobilized onto porous glass beads by covalent coupling using activation by a silane reagent and glutaraldehyde before adding the enzyme. The im...

    journal_title：Enzyme and microbial technology

    pub_type： 杂志文章

    doi：10.1016/s0141-0229(00)00342-2

    authors： Ettalibi M,Baratti JC

    更新日期：2001-05-07 00:00:00