Peptide synthesis by proteases in organic solvents: medium effect on substrate specificity.

abstract：:Data and a semi-empirical model are presented that describe the affinity interaction of yeast cells with a Concanavalin A derivatised surface. The model uses 3 parameters to describe the time course of cell attachment from a flowing suspension of yeast cells, over a range of flow rates, and gives an effective global f...

journal_title：Enzyme and microbial technology

authors： Ming F,Eisenthal R,Whish WJ,Hubble J

更新日期：2000-02-01 00:00:00

abstract：:In this study, distillery wastewater was treated by dark fermentation or photofermentation alone, and by sequential dark and photofermentation processes using anaerobic saccharolytic consortium and purple nonsulfur bacteria. Combination of dark and photofermentation resulted in the maximal H2 yield of 17.6L/L of disti...

journal_title：Enzyme and microbial technology

authors： Laurinavichene T,Tekucheva D,Laurinavichius K,Tsygankov A

更新日期：2018-03-01 00:00:00

abstract：:Three threonine aldolases (TAs) were cloned and overexpressed in Escherichia coli (Aeromonas jandaeil-allo-threonine aldolase, E. colil-threonine aldolase and Thermotoga maritimal-allo-threonine aldolase). A Design of Experiments strategy was used to identify optimal reaction conditions for each enzyme. These conditio...

journal_title：Enzyme and microbial technology

authors： Beaudoin SF,Hanna MP,Ghiviriga I,Stewart JD

更新日期：2018-12-01 00:00:00

abstract：:Effects of pulse and step changes in major metabolite concentrations such as glucose, glutamine, lactic acid, and ammonium on behavior of T-cell hybridomas in continuous culture were investigated in order to develop a strategy to maximize specific rate of lymphokine formation. Step increases in glucose, lactic acid, a...

journal_title：Enzyme and microbial technology

authors： Shaughnessy TS,Kargi F

更新日期：1990-09-01 00:00:00

abstract：:We have designed an electrochemical DNA biosensor based on stem-loop structured probes for enzymatic detection of Pseudomonas aeruginosa 16S ribosomal RNA (rRNA) in composting degradation. The probe modified with a thiol at its 5' end and a biotin at its 3' end was immobilized on a gold electrode through self-assembly...

journal_title：Enzyme and microbial technology

authors： Liu C,Zeng GM,Tang L,Zhang Y,Li YP,Liu YY,Li Z,Wu MS,Luo J

更新日期：2011-08-10 00:00:00

abstract：:A lipase, LipG7, has been purified from the Antarctic filamentous fungus Geomyces sp. P7 which was found to be cold-adapted and able to retain/regain its activity after heat denaturation. The LipG7 exhibits 100% residual activity following 1h incubation at 100°C whilst simultaneously showing kinetic adaptations to col...

journal_title：Enzyme and microbial technology

authors： Florczak T,Daroch M,Wilkinson MC,Białkowska A,Bates AD,Turkiewicz M,Iwanejko LA

更新日期：2013-06-10 00:00:00

abstract：:D-glucuronic acid (GlcUA) is an important intermediate with numerous applications in the food, cosmetics, and pharmaceutical industries. Its biological production routes which employ myo-inositol oxygenase (MIOX) as the key enzyme are attractive. In this study, five diverse MIOX-encoding genes, from Cryptococcus neofo...

journal_title：Enzyme and microbial technology

authors： Teng F,You R,Hu M,Liu W,Wang L,Tao Y

更新日期：2019-08-01 00:00:00

abstract：:The parameters of biosensors based on the yeast strains Candida maltosa VKM Y-2359, Candida blankii VKM Y-2675, and Debaryomyces hansenii VKM Y-2482 for biochemical oxygen demand (BOD) detection are compared. The catalytic activity of the strains was analyzed in relation to the growth phase. The possibility of using D...

journal_title：Enzyme and microbial technology

authors： Arlyapov V,Kamanin S,Ponamoreva O,Reshetilov A

更新日期：2012-04-05 00:00:00

abstract：:Advantages in maltose fermentation at extremely low temperatures (5-10°C) using an alcohol resistant and cryotolerant yeast strain (Saccharomyces cerevisiae AXAZ-1) immobilized on porous cellulosic material (or tubular cellulose, TC), produced by delignification of wood sawdust, are reported. Pure maltose and glucose ...

journal_title：Enzyme and microbial technology

authors： Ganatsios V,Koutinas AA,Bekatorou A,Kanellaki M,Nigam P

更新日期：2014-11-01 00:00:00

abstract：:Phospholipase C (PLC) activity secreted by bacteria as a virulence factor is commonly detected by use of the artificial substrate p-nitrophenylphosphorylcholine (p-NPPC). We examined several commercially available enzymes (phosphodiesterases, phosphomonoesterases, phospholipase A, lipase, protease) for their hydrolyti...

journal_title：Enzyme and microbial technology

authors： Fliegera A,Gong S,Faigle M,Neumeister B

更新日期：2000-03-01 00:00:00

abstract：:Enzymatic production of cephalexin using immobilized penicillin G acylase was studied. The factors that affect the conversion of 7-amino-3-deacetoxycephalosporanic acid (7-ADCA) to cephalexin, such as pH; temperature; concentrations of 7-ADCA, D(-)-phenylglycine methyl ester (PGME), and immobilized penicillin G acylas...

journal_title：Enzyme and microbial technology

authors： Maladkar NK

更新日期：1994-08-01 00:00:00

abstract：:Corynebacterium glutamicum is an important industrial organism for the production of a variety of biological commodities. We discovered a promoter encoded by the gene NCgl2319 in C. glutamicum, which could be induced by benzyl alcohol, could be used as an efficient tunable expression system. In initial attempts, this ...

journal_title：Enzyme and microbial technology

authors： Liu X,Zhao Z,Dong G,Li Y,Peng F,Liu C,Zhang F,Linhardt RJ,Yang Y,Bai Z

更新日期：2020-11-01 00:00:00

abstract：:Various recombinant proteins can be produced by the yeast Saccharomyces cerevisiae cell factories; therefore, efficient recombinant protein production techniques are desirable. In this study, to establish an efficient recombinant protein production technique in S. cerevisiae, the secretory production of recombinant pr...

journal_title：Enzyme and microbial technology

authors： Sasaki Y,Mitsui R,Yamada R,Ogino H

更新日期：2019-02-01 00:00:00

abstract：:Bacterial spores maintain metabolic dormancy and have high resistance to external pressure. Germination requires degradation of the spore cortex and the participation of germination-specific cortex-lytic enzymes (GSLEs). Previously reported GSLEs have been identified in bacteria and facilitate germination. In this stu...

journal_title：Enzyme and microbial technology

authors： Fu Y,Liang L,Deng S,Wu Y,Yuan Y,Gao M

更新日期：2020-12-01 00:00:00

abstract：:Microorganism selection is critical to deglycosylation in soybean fermentation for producing beneficial phytochemicals. This study investigated isoflavone bioconversion in soybean extract inoculated with Lactobacillus plantarum K2-12 and Lactobacillus curvatus JD0-31 exhibiting different enzyme activities. L. plantaru...

journal_title：Enzyme and microbial technology

authors： Lim YJ,Lim B,Kim HY,Kwon SJ,Eom SH

更新日期：2020-01-01 00:00:00

abstract：:Progesterone 5β-reductases (P5βRs) are involved in 5β-cardenolide formation by stereo-specific reduction of the △4,5 double bond of steroid precursors. In this study a steroid 5β-reductase was identified in Capsella rubella (CrSt5βR1) and its function in steroid 5β-reduction was validated experimentally. CrSt5βR1 is c...

journal_title：Enzyme and microbial technology

authors： Li Y,Pan H,Chang Y,Dong N,Zou L,Liang P,Tian W,Chang Z

更新日期：2020-03-01 00:00:00

abstract：:Even though many enzyme processes occur at the interface of an insoluble substrate, these reactions are generally much less studied than homogenous enzyme reactions in the aqueous bulk. Interfacial (or heterogeneous) enzyme reactions involve several reaction steps, and the established experimental approach to elucidat...

journal_title：Enzyme and microbial technology

authors： Olsen JP,Kari J,Borch K,Westh P

更新日期：2017-10-01 00:00:00

abstract：:A method to stably immobilize microbes on electrodes was developed. Resting cells of Methylobacterium extorquens AM1(MeAM1) were caged within multiwalled carbon nanotubes (MWNTs)by adding the cells to a water dispersion of MWNTs then allowing the resulting mixture to dry on electrodes. The MeAM1-MWCNTs electrode thus ...

journal_title：Enzyme and microbial technology

authors： Xia HQ,Sakai K,Kitazumi Y,Shirai O,Takagi K,Kano K

更新日期：2018-10-01 00:00:00

abstract：:Two acidic β-glucosidases (βGI and βGII) from the brown rot fungus Fomitopsis palustris were purified to homogeneity by several chromatographic steps. βGI and βGII had molecular weights of 130 and 213 kDa, respectively, and exhibited optimum activity at pH 2.5 and 55°C. The K(m) values of βGI and βGII for p-nitropheny...

journal_title：Enzyme and microbial technology

authors： Okamoto K,Sugita Y,Nishikori N,Nitta Y,Yanase H

更新日期：2011-04-07 00:00:00

abstract：:The cell wall integrity and stress response component (WSC) domain was first described in the Wsc-family protein of the yeast Saccharomyces cerevisiae, and later found in diverse eukaryotic organisms. Due solely to their presence in the Wsc-family proteins working as a plasma membrane sensor for surface stress and in ...

journal_title：Enzyme and microbial technology

authors： Oide S,Tanaka Y,Watanabe A,Inui M

更新日期：2019-06-01 00:00:00

abstract：:The activities (at pH 7 and 50 degrees C) of purified EGV (Humicola insolens) and CenA (Cellulomonas fimi) were determined on cotton fabrics at high and low levels of mechanical agitation. Similar activity measurements were also made by using the core domains of these cellulases. Activity experiments suggested that th...

journal_title：Enzyme and microbial technology

authors： Azevedo H,Bishop D,Cavaco-Paulo A

更新日期：2000-08-01 00:00:00

abstract：:Numerous studies have focused on how to obtain high yield of sophorolipids using low-cost materials as substrates, and there has been various work on the experimental methods for purifying lactonic sophorolipids. These studies have not yet obtained satisfied results in combining a low-cost fermentation process and the...

journal_title：Enzyme and microbial technology

authors： Yang X,Zhu L,Xue C,Chen Y,Qu L,Lu W

更新日期：2012-12-10 00:00:00

abstract：:The lipases from Thermomyces lanuginosus and Pseudomonas cepacia have been immobilized on octyl and cyanogen bromide (CNBr) agarose beads. The immobilization on octyl-agarose is slowed with increasing ionic strength, while the immobilization on CNBr is not significantly affected by the ionic strength. The inhibition o...

journal_title：Enzyme and microbial technology

authors： Manoel EA,Dos Santos JC,Freire DM,Rueda N,Fernandez-Lafuente R

更新日期：2015-04-01 00:00:00

abstract：:l-asparaginase is a pharmaceutically and industrially important enzyme as it has potential to treat different cancers and inhibit acrylamide formation in fried and baked food products. In the present study, an attempt was made to screen for new and novel l-asparaginase producers using a widely applied phenol red and b...

journal_title：Enzyme and microbial technology

authors： Mihooliya KN,Nandal J,Swami L,Verma H,Chopra L,Sahoo DK

更新日期：2017-12-01 00:00:00

abstract：:Alginate was evaluated as an immobilization matrix for enzyme-catalyzed reactions in organic solvents. In contrast to most hydrogels, calcium alginate was found to be stable in a range of organic solvents and to retain the enzyme inside the gel matrix. In hydrophobic solvents, the alginate gel (greater than 95% water)...

journal_title：Enzyme and microbial technology

authors： Hertzberg S,Kvittingen L,Anthonsen T,Skjåk-Braek G

更新日期：1992-01-01 00:00:00

abstract：:The effect of treatment with various intercalating dyes on the ability to produce antibiotics in Micromonospora rosaria and Micromonospora purpurea was studied. Treatment with acriflavine resulted in a high frequency loss of antibiotic productivity in both species. In M. rosaria, the loss of antibiotic-producing abili...

journal_title：Enzyme and microbial technology

authors： Kim KS,Pai HS,Lee SY,Ryu DD

更新日期：1990-08-01 00:00:00

abstract：:Hydrolysis of lignocellulosic biomass depends on the concerted actions of cellulases and accessory proteins. In this work we examined the combined action of two auxiliary proteins from the brown rot fungus Gloeophyllum trabeum: a family AA9 lytic polysaccharide monooxygenase (GtLPMO) and a GH10 xylanase (GtXyn10A). Th...

journal_title：Enzyme and microbial technology

authors： Sanhueza C,Carvajal G,Soto-Aguilar J,Lienqueo ME,Salazar O

更新日期：2018-06-01 00:00:00

abstract：:The gene encoding a cold-adapted phospholipase A(1) (PLA(1)) from a psychrotrophic, glacier soil bacterium Serratia sp. xjF1 was cloned by two-step PCR (general PCR and TAIL-PCR). The full-length fragment comprised two open reading frames plA and plS. The gene product of plA encoding 320 amino acids with a molecular w...

journal_title：Enzyme and microbial technology

authors： Fu J,Huang H,Meng K,Yuan T,Yao B,Shi Y,Ouyang P

更新日期：2008-01-01 00:00:00

abstract：:In this work, a biosensor using a glassy carbon electrode modified with gold nanoparticles (AuNPs) and tyrosinase (Tyr) within a dihexadecylphosphate film is proposed. Cystamine and glutaraldehyde crosslinking agents were used as a support for Tyr immobilization. The proposed biosensor was characterized by scanning el...

journal_title：Enzyme and microbial technology

authors： Campanhã Vicentini F,Garcia LL,Figueiredo-Filho LC,Janegitz BC,Fatibello-Filho O

更新日期：2016-03-01 00:00:00

abstract：:Marine microbes have gained significant attention as potential biofactories for broad spectrum bioactive compounds. In the recent years, bioactive biosurfactants have warranted renewed interest from both environmental and clinical sectors as anti-biofilm agents due to their excellent properties of dispersing microbial...

journal_title：Enzyme and microbial technology

authors： Balan SS,Mani P,Kumar CG,Jayalakshmi S

更新日期：2019-01-01 00:00:00