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A biosensor based on gold nanoparticles, dihexadecylphosphate, and tyrosinase for the determination of catechol in natural water.

Abstract：

:In this work, a biosensor using a glassy carbon electrode modified with gold nanoparticles (AuNPs) and tyrosinase (Tyr) within a dihexadecylphosphate film is proposed. Cystamine and glutaraldehyde crosslinking agents were used as a support for Tyr immobilization. The proposed biosensor was characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and cyclic voltammetry in the presence of catechol. The determination of catechol was carried out by amperometry and presented a linear concentration range from 2.5×10(-6) to 9.5×10(-5)molL(-1) with a detection limit of 1.7×10(-7)molL(-1). The developed biosensor showed good repeatability and stability. Moreover, this novel amperometric method was successfully applied in the determination of catechol in natural water samples. The results were in agreement with a 95% confidence level for those obtained using the official spectrophotometric method.

journal_name

Enzyme Microb Technol

journal_title

Enzyme and microbial technology

authors

Campanhã Vicentini F,Garcia LL,Figueiredo-Filho LC,Janegitz BC,Fatibello-Filho O

doi

10.1016/j.enzmictec.2015.12.004

subject

Has Abstract

pub_date

2016-03-01 00:00:00

pages

17-23

eissn

0141-0229

issn

1879-0909

pii

S0141-0229(15)30087-9

journal_volume

84

pub_type

杂志文章

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