Enhanced detection of RNA by MMLV reverse transcriptase coupled with thermostable DNA polymerase and DNA/RNA helicase.

abstract：:An approach was developed to enhance the efficiency for the bioconversion of 1-(3-hydroxyphenyl)-2-(methyamino)-ethanone to (R)-phenylephrine. The strain Serratia marcescens N10612, giving the benefit of 99% enantiomeric excess in (R)-PE conversion, was used. The fermentation was devised to harvest cells with high hyd...

journal_title：Enzyme and microbial technology

authors： Kan SC,Zang CZ,Yeh CW,Chang WF,Lin CC,Hung TH,Shieh CJ,Liu YC

更新日期：2016-05-01 00:00:00

abstract：:A novel kinetic method was developed for the quantitation of α-ketoglutaric acid (AKG) in cardioplegic solution and athletic supplements. The assay relied on an enzymatic transamination of AKG and d-4-hydroxyphenylglycine to form 4-hydroxybenzoylformic acid and l-glutamic acid using d-phenylglycine aminotransferase. S...

journal_title：Enzyme and microbial technology

authors： Rojanarata T,Laiwattanapaisal W,Ngawhirunpat T,Opanasopit P,Worrawethanakul K,Chantadee T,Fuangwattana T

更新日期：2018-09-01 00:00:00

abstract：:The parameters of biosensors based on the yeast strains Candida maltosa VKM Y-2359, Candida blankii VKM Y-2675, and Debaryomyces hansenii VKM Y-2482 for biochemical oxygen demand (BOD) detection are compared. The catalytic activity of the strains was analyzed in relation to the growth phase. The possibility of using D...

journal_title：Enzyme and microbial technology

authors： Arlyapov V,Kamanin S,Ponamoreva O,Reshetilov A

更新日期：2012-04-05 00:00:00

abstract：:Production of laccase activity by nine strains of Chalara paradoxa isolated from olive mill wastewater disposal ponds were studied. Enzyme extracts obtained from cultured broths by adsorption on hydroxyapatite showed a single band of laccase activity on ABTS after polyacrylamide gel electrophoresis (PAGE). They showed...

journal_title：Enzyme and microbial technology

authors： Robles A,Lucas R,de Cienfuegos GA,Gálvez A

更新日期：2000-04-01 00:00:00

abstract：:Oxygen uptake rate and oxygen mass transfer rate have been studied during xanthan gum production process in stirred tank bioreactor. Empirical equations for the oxygen mass transfer coefficient have been obtained taking into account several variables such as air flow rate, stirrer speed and apparent viscosity. Oxygen ...

journal_title：Enzyme and microbial technology

authors： García-Ochoa F,Castro EG,Santos VE

更新日期：2000-11-15 00:00:00

abstract：:The heat and mass transfer have been proved to be the important factors in air pressure pulsation for cellulase production. However, as process of enzyme secretion, the cellulase formation has not been studied in the view of microorganism metabolism and metabolic key enzyme activity under air pressure pulsation condit...

journal_title：Enzyme and microbial technology

authors： Chen H,Shao M,Li H

更新日期：2014-03-05 00:00:00

abstract：:The recombinant shuttle plasmid pCPPS-31, which confers carboxymethylcellulase (CMCase) production and neomycin resistance (Ner), was segregationally but not structurally unstable in both B. subtilis (50-60%) and Escherichia coli (80-90%) when grown in serial batch cultures for 72 h diluting 1:1,000 with fresh medium ...

journal_title：Enzyme and microbial technology

authors： Vyas VV,Gupta S,Sharma P

更新日期：1994-03-01 00:00:00

abstract：:This work presents the purification and characterization of an extracellular alpha-amylase (1,4-alpha-D-glucan glucanohydrolase, EC 3.2.1.1) produced by a new lactic acid bacterium: Lactobacillus manihotivorans able to produce L(+) lactic acid from starch. The molecular weight was found to be 135 kDa. The temperature ...

journal_title：Enzyme and microbial technology

authors： Aguilar G,Morlon-Guyot J,Trejo-Aguilar B,Guyot JP

更新日期：2000-09-01 00:00:00

abstract：:Even though many enzyme processes occur at the interface of an insoluble substrate, these reactions are generally much less studied than homogenous enzyme reactions in the aqueous bulk. Interfacial (or heterogeneous) enzyme reactions involve several reaction steps, and the established experimental approach to elucidat...

journal_title：Enzyme and microbial technology

authors： Olsen JP,Kari J,Borch K,Westh P

更新日期：2017-10-01 00:00:00

abstract：:D-glucuronic acid (GlcUA) is an important intermediate with numerous applications in the food, cosmetics, and pharmaceutical industries. Its biological production routes which employ myo-inositol oxygenase (MIOX) as the key enzyme are attractive. In this study, five diverse MIOX-encoding genes, from Cryptococcus neofo...

journal_title：Enzyme and microbial technology

authors： Teng F,You R,Hu M,Liu W,Wang L,Tao Y

更新日期：2019-08-01 00:00:00

abstract：:A methyl parathion hydrolase (MPH) gene, bjmpd, was cloned from a newly isolated MP-degrading bacterial strain, Burkholderia jiangsuensis MP-1T and heterologously expressed in Escherichia coli BL21 (DE3). Although the amino acid sequence of the bjmpd-encoded enzyme, named BjMPH, differed from that of MPH from Pseudomo...

journal_title：Enzyme and microbial technology

authors： Liu XY,Chen FF,Li CX,Luo XJ,Chen Q,Bai YP,Xu JH

更新日期：2016-11-01 00:00:00

abstract：:Bacterial chitinases recruited multiple accessory domains for the conversion of recalcitrant polysaccharides to simple soluble sugars/amino sugars. Here, we report detailed properties of a multi-domain GH18 chitinase from Enterobacter cloacae subsp. cloacae (EcChi2) that preferred β-chitin as substrate. EcChi2 exhibit...

journal_title：Enzyme and microbial technology

authors： Mallakuntla MK,Podile AR

更新日期：2021-02-01 00:00:00

abstract：:GH11 xylanase XynJ from Bacillus sp. strain 41M-1 has a β-jellyroll fold composed of eight β strands with a deep active-site cleft. We hypothesized that the thermostability of XynJ will increase if the flexibility of the β strands in the jellyroll structure is decreased without impairing activity. To verify this hypot...

journal_title：Enzyme and microbial technology

authors： Takita T,Nakatani K,Katano Y,Suzuki M,Kojima K,Saka N,Mikami B,Yatsunami R,Nakamura S,Yasukawa K

更新日期：2019-11-01 00:00:00

abstract：:Kinetic resolution of sec-alcohols is a green process with biocatalyst. Candida antarctica lipase B (CALB) displayed on Pichia pastoris cell-surface (Pp-CALB) was characterized in kinetic resolution of sec-alcohols with different structures. The reaction parameters including acyl donors, molar ratio of substrates, sol...

journal_title：Enzyme and microbial technology

authors： Zhang K,Pan Z,Diao Z,Liang S,Han S,Zheng S,Lin Y

更新日期：2018-03-01 00:00:00

abstract：:The activities (at pH 7 and 50 degrees C) of purified EGV (Humicola insolens) and CenA (Cellulomonas fimi) were determined on cotton fabrics at high and low levels of mechanical agitation. Similar activity measurements were also made by using the core domains of these cellulases. Activity experiments suggested that th...

journal_title：Enzyme and microbial technology

authors： Azevedo H,Bishop D,Cavaco-Paulo A

更新日期：2000-08-01 00:00:00

abstract：:Musky macrolactones are an important group of compounds used in high-valued perfumery. An enzymatic cascade reaction including cytochrome P450 hydroxylase and lipase was explored to biosynthesize musky macrolactones. Firstly, fatty acids were hydroxylated by P450 hydroxylase to produce the corresponding ω-hydroxy fatt...

journal_title：Enzyme and microbial technology

authors： Meng S,Guo J,Li Z,Nie K,Xu H,Tan T,Liu L

更新日期：2019-12-01 00:00:00

abstract：:Ergosterol, like cholesterol, has many beneficial physiological activities, and because it has no adverse clinical problem with cholesterol, it is a substance that can be used as a biomaterial in the food and cosmetics industry. However, ergosterol has low oil solubility and is easily crystallized, which is problemati...

journal_title：Enzyme and microbial technology

authors： Park SH,Kim HK

更新日期：2020-09-01 00:00:00

abstract：:A method to stably immobilize microbes on electrodes was developed. Resting cells of Methylobacterium extorquens AM1(MeAM1) were caged within multiwalled carbon nanotubes (MWNTs)by adding the cells to a water dispersion of MWNTs then allowing the resulting mixture to dry on electrodes. The MeAM1-MWCNTs electrode thus ...

journal_title：Enzyme and microbial technology

authors： Xia HQ,Sakai K,Kitazumi Y,Shirai O,Takagi K,Kano K

更新日期：2018-10-01 00:00:00

abstract：:Three threonine aldolases (TAs) were cloned and overexpressed in Escherichia coli (Aeromonas jandaeil-allo-threonine aldolase, E. colil-threonine aldolase and Thermotoga maritimal-allo-threonine aldolase). A Design of Experiments strategy was used to identify optimal reaction conditions for each enzyme. These conditio...

journal_title：Enzyme and microbial technology

authors： Beaudoin SF,Hanna MP,Ghiviriga I,Stewart JD

更新日期：2018-12-01 00:00:00

abstract：:Data and a semi-empirical model are presented that describe the affinity interaction of yeast cells with a Concanavalin A derivatised surface. The model uses 3 parameters to describe the time course of cell attachment from a flowing suspension of yeast cells, over a range of flow rates, and gives an effective global f...

journal_title：Enzyme and microbial technology

authors： Ming F,Eisenthal R,Whish WJ,Hubble J

更新日期：2000-02-01 00:00:00

abstract：:Effects of pulse and step changes in major metabolite concentrations such as glucose, glutamine, lactic acid, and ammonium on behavior of T-cell hybridomas in continuous culture were investigated in order to develop a strategy to maximize specific rate of lymphokine formation. Step increases in glucose, lactic acid, a...

journal_title：Enzyme and microbial technology

authors： Shaughnessy TS,Kargi F

更新日期：1990-09-01 00:00:00

abstract：:By using very active and very stable penicillin G acylase (PGA)--agarose derivatives we have studied the industrial design of equilibrium-controlled synthesis of lactamic antibiotics. In the presence of high concentrations of organic cosolvents we have carried out the direct enzymatic condensation of phenylacetic acid...

journal_title：Enzyme and microbial technology

authors： Fernandez-Lafuente R,Rosell CM,Guisán JM

更新日期：1991-11-01 00:00:00

abstract：:Recombinant plasmid pCED3 [confers beta-galactosidase production (LacZ+) and kanamycin resistance (Kmr)] in Bacillus subtilis was found to be both segregationally and structurally unstable. Since many solutions to segregational instability are already available, the problem of structural instability was specifically a...

journal_title：Enzyme and microbial technology

authors： Shoham Y,Demain AL

更新日期：1990-05-01 00:00:00

abstract：:The hydrolytic activity of a thermophilic cyclomaltodextrinase (CMD) from Anoxybacillus flavithermus ZNU-NGA and a representative single mutant were investigated against soluble substrates including α-, β- and γ-cyclomaltodestrines (CDs). Based on the occurrence of arginine (Arg) at position 403 in some homologue prot...

journal_title：Enzyme and microbial technology

authors： Mirzaee Z,Jafarian V,Shirdel SA,Khalifeh K

更新日期：2019-12-01 00:00:00

abstract：:l-ribose and d-tagatose are biochemically synthesized using sugar isomerases. The l-arabinose isomerase gene from Shigella flexneri (Sf-AI) was cloned and expressed in Escherichia coli BL-21. Sf-AI was applied for the bioproduction of d-tagatose from d-galactose. l-ribose synthesis was performed by two step isomerizat...

journal_title：Enzyme and microbial technology

authors： Patel MJ,Akhani RC,Patel AT,Dedania SR,Patel DH

更新日期：2017-02-01 00:00:00

abstract：:The substrate specificities of alpha-chymotrypsin and subtilisins for peptide synthesis in hydrophilic organic solvents were investigated. Chymotrypsin exhibited high specificity to aromatic amino acids as acyl donors, while subtilisin Carlsberg and subtilisin BPN' were specific to aromatic and neutral aliphatic amino...

journal_title：Enzyme and microbial technology

authors： Nagashima T,Watanabe A,Kise H

更新日期：1992-10-01 00:00:00

abstract：:The characterization of the S' subsite specificity of native and ethylated alpha-chymotrypsin has been studied via acyl transfer reaction in acetonitrile containing 10 vol% of water. Using Ac-Tyr-OEt as acyl donor, we investigated the partitioning of acyl-chymotrypsins between water and amino acid and peptide-derived ...

journal_title：Enzyme and microbial technology

authors： Cerovský V,Jakubke HD

更新日期：1994-07-01 00:00:00

abstract：:Different whole-cell immobilization methods and conditions were tested for the delta 1-dehydrogenation of 6-alpha-methyl-hydrocortisone-21-acetate with Arthrobacter simplex cells, in n-decane-1-ol and chloroform. Among the entrapment methods, polyurethane foams gave the best productivity of n-decane-1-ol, but could no...

journal_title：Enzyme and microbial technology

authors： Pinheiro HM,Cabral JM

更新日期：1992-08-01 00:00:00

abstract：:In the present work, NAD(+)-dependent formate dehydrogenase (FDH), encoded by fdh gene from Candida boidinii was successfully displayed on Escherichia coli cell surface using ice nucleation protein (INP) from Pseudomonas borealis DL7 as an anchoring protein. Localization of matlose binding protein (MBP)-INP-FDH fusion...

journal_title：Enzyme and microbial technology

authors： Liu A,Feng R,Liang B

更新日期：2016-09-01 00:00:00

abstract：:Astragalin (kaempferol-3-O-β-D-glucopyranoside, Ast) glucosides were synthesized by the acceptor reaction of a dextransucrase produced by Leuconostoc mesenteroides B-512FMCM with astragalin and sucrose. Each glucoside was purified and their structures were assigned as kaempferol-3-O-β-D-glucopyranosyl-(1→3)-O-α-D-gluc...

journal_title：Enzyme and microbial technology

authors： Kim GE,Kang HK,Seo ES,Jung SH,Park JS,Kim DH,Kim DW,Ahn SA,Sunwoo C,Kim D

更新日期：2012-01-05 00:00:00