Critical evaluation of p-nitrophenylphosphorylcholine (p-NPPC) as artificial substrate for the detection of phospholipase C*

abstract：:The recombinant shuttle plasmid pCPPS-31, which confers carboxymethylcellulase (CMCase) production and neomycin resistance (Ner), was segregationally but not structurally unstable in both B. subtilis (50-60%) and Escherichia coli (80-90%) when grown in serial batch cultures for 72 h diluting 1:1,000 with fresh medium ...

journal_title：Enzyme and microbial technology

authors： Vyas VV,Gupta S,Sharma P

更新日期：1994-03-01 00:00:00

abstract：:Filamentous fungal secretomes comprise highly dynamic sets of proteins, including multiple carbohydrate active enzymes (CAZymes) which are able to hydrolyze plant biomass polysaccharides into products of biotechnological interest such as fermentable sugars. In recent years, proteomics has been used to identify and qua...

journal_title：Enzyme and microbial technology

authors： Cologna NMD,Gómez-Mendoza DP,Zanoelo FF,Giannesi GC,Guimarães NCA,Moreira LRS,Filho EXF,Ricart CAO

更新日期：2018-02-01 00:00:00

abstract：:Two novel glycosyl hydrolase family 5 (GH5) β-mannanases (AoMan5A and AoMan5B) were identified from Aspergillus oryzae RIB40 by genome mining. The AoMan5A contains a predicted family 1 carbohydrate binding module (CBM-1), located at its N-terminal. The AoMan5A, AoMan5B and truncated mutant AoMan5AΔCL (truncating the N...

journal_title：Enzyme and microbial technology

authors： Tang CD,Shi HL,Tang QH,Zhou JS,Yao LG,Jiao ZJ,Kan YC

更新日期：2016-11-01 00:00:00

abstract：:This work presents the purification and characterization of an extracellular alpha-amylase (1,4-alpha-D-glucan glucanohydrolase, EC 3.2.1.1) produced by a new lactic acid bacterium: Lactobacillus manihotivorans able to produce L(+) lactic acid from starch. The molecular weight was found to be 135 kDa. The temperature ...

journal_title：Enzyme and microbial technology

authors： Aguilar G,Morlon-Guyot J,Trejo-Aguilar B,Guyot JP

更新日期：2000-09-01 00:00:00

abstract：:Bacterial chitinases recruited multiple accessory domains for the conversion of recalcitrant polysaccharides to simple soluble sugars/amino sugars. Here, we report detailed properties of a multi-domain GH18 chitinase from Enterobacter cloacae subsp. cloacae (EcChi2) that preferred β-chitin as substrate. EcChi2 exhibit...

journal_title：Enzyme and microbial technology

authors： Mallakuntla MK,Podile AR

更新日期：2021-02-01 00:00:00

abstract：:Human interferon alpha 2b (IFN α2b) is a type I interferon exhibiting antiviral, anti-proliferative and immunomodulatory activities. The clinical outcome of the approved recombinant human IFN α2b drugs in the market suffers from short plasma half-life, rapid clearance and other side effects. Human IFN α2b expression i...

journal_title：Enzyme and microbial technology

authors： Katla S,Yoganand KNR,Hingane S,Ranjith Kumar CT,Anand B,Sivaprakasam S

更新日期：2019-09-01 00:00:00

abstract：:Addition of non-ionic surfactants (NIS) is known to accelerate enzymatic lignocellulose hydrolysis. The mechanism behind this accelerating effect is still not elucidated but has been hypothesized to originate from favorable NIS-lignin interactions which alleviate non-productive adsorption of cellulases to lignin. In t...

journal_title：Enzyme and microbial technology

authors： Olsen SN,Bohlin C,Murphy L,Borch K,McFarland KC,Sweeny MD,Westh P

更新日期：2011-09-10 00:00:00

abstract：:Operational stability under high temperature is required for enzyme application in industrial processes. Error-prone PCR and B-factor analysis were employed to enhance the thermostability of a xylanase from GH family 11 in this study. Based on the top 10 mutants screened from the random mutation libraries, mutant Xyn3...

journal_title：Enzyme and microbial technology

authors： Xing H,Zou G,Liu C,Chai S,Yan X,Li X,Liu R,Yang Y,Zhou Z

更新日期：2021-02-01 00:00:00

abstract：:The primary aim of this study was to decipher the catalytic functions of the NHase with wide substrate spectra from Rhodococcus ruber CGMCC3090 by computer modeling and substrate docking. 3D structure model of the enzyme was built by computer modeling to obtain the optimal structure. The larger binding site cavity (55...

journal_title：Enzyme and microbial technology

authors： Wang S,Dai Y,Wang J,Shen Y,Zhai Y,Zheng H,Wang M

更新日期：2013-02-05 00:00:00

abstract：:Before applying nanotechnologies in biomedical and environmental areas it is advised to study interactions of nanoparticles and other nanomaterials with biomacromolecule present in living system. Moreover there is scarcity of reports on interactions between nanoparticles and biomaterials. In present report a rapid, ec...

journal_title：Enzyme and microbial technology

authors： Patil CD,Borase HP,Suryawanshi RK,Patil SV

更新日期：2016-10-01 00:00:00

abstract：:Acetobacter pasteurianus oxidizes glycidol with high activity, comparable to the oxidation of ethanol. The organism has a preference for the S-enantiomer, and the kinetic resolution process obeys a simple relationship, indicating an enantiomeric ratio (E) of 19. The compound is converted into glycidic acid, although a...

journal_title：Enzyme and microbial technology

authors： Geerlof A,Jongejan JA,van Dooren TJ,Racemakers-Franken PC,van den Tweel WJ,Duine JA

更新日期：1994-12-01 00:00:00

abstract：:Electricity production from carbon monoxide (CO) is demonstrated in a single chamber microbial fuel cell (MFC) with a CoTMPP-based air cathode. The MFC was inoculated with anaerobic sludge and continuously sparged with CO as a sole carbon source. Volumetric power output was maximized at a CO flow rate of 4.8LLR(-1)d(-...

journal_title：Enzyme and microbial technology

authors： Mehta P,Hussain A,Tartakovsky B,Neburchilov V,Raghavan V,Wang H,Guiot SR

更新日期：2010-05-05 00:00:00

abstract：:Magnetosomes are intracellular structures produced by magnetotactic bacteria and are magnetic nanoparticles surrounded by a lipid bilayer membrane. Magnetosomes reportedly possess intrinsic enzyme mimetic activity similar to that found in horseradish peroxidase (HRP) and can scavenge reactive oxygen species depending ...

journal_title：Enzyme and microbial technology

authors： Li K,Chen C,Chen C,Wang Y,Wei Z,Pan W,Song T

更新日期：2015-05-01 00:00:00

abstract：:Cystathionine γ-lyase (CGL) is a key enzyme in the methionine-cysteine cycle in all living organisms forming cysteine, α-ketobutyrate and ammonia via homocysteine and cystathionine intermediates. Although, human and plant CGLs have been extensively studied at the molecular and mechanistic levels, there has been little...

journal_title：Enzyme and microbial technology

authors： El-Sayed AS,Abdel-Azeim S,Ibrahim HM,Yassin MA,Abdel-Ghany SE,Esener S,Ali GS

更新日期：2015-12-01 00:00:00

abstract：:The yeast Pichia pastoris is a widely used host for recombinant protein expression, and has recently been engineered for whole-cell biocatalysis. The inducible P(AOX) and constitutive P(GAP) promoters are commonly employed. In this study, the S-adenosyl-L-methionine (SAM) biosynthesis and degradation efficiency of two...

journal_title：Enzyme and microbial technology

authors： Hu X,Chu J,Zhang S,Zhuang Y

更新日期：2014-02-05 00:00:00

abstract：:Developing a strain with high docosahexaenoic acid (DHA) yield and stable fermenting-performance is an imperative way to improve DHA production using Aurantiochytrium sp., a microorganism with two fatty acid synthesis pathways: polyketide synthase (PKS) pathway and Type I fatty acid synthase (FAS) pathway. This study ...

journal_title：Enzyme and microbial technology

authors： Cheng YR,Sun ZJ,Cui GZ,Song X,Cui Q

更新日期：2016-11-01 00:00:00

abstract：:We demonstrate the feasibility of growing crystals of protein in volumes as small as 1 nanoliter. Advances in the handling of very small volumes (i.e. through inkjet and other technologies) open the way towards fully automated systems. The rationale for these experiments is the desire to develop a system that speeds u...

journal_title：Enzyme and microbial technology

authors： Kuil ME,Bodenstaff ER,Hoedemaeker FJ,Abrahams JP

更新日期：2002-03-13 00:00:00

abstract：:Genetic engineering has been used to modify the proportion of different cellulases produced by a hypercellulolytic Trichoderma reesei mutant strain. A general expression vector, pAMH110, containing the promoter and terminator sequences of the strongly expressed main cellobiohydrolase 1 (cbh1) gene was used to overexpr...

journal_title：Enzyme and microbial technology

authors： Harkki A,Mäntylä A,Penttilä M,Muttilainen S,Bühler R,Suominen P,Knowles J,Nevalainen H

更新日期：1991-03-01 00:00:00

abstract：:Nine proteins with lignin peroxidase activity were separated from cultures of Phanerochaete chrysosporium INA-12 in glycerol as carbon source and non-nitrogen limited. Four lignin peroxidase isozymes (4, 5, 8, 9) were purified and characterized. Although differences in kinetic parameters could be shown, antibody react...

journal_title：Enzyme and microbial technology

authors： Odier E,Delattre M

更新日期：1990-06-01 00:00:00

abstract：:The lipases from Thermomyces lanuginosus and Pseudomonas cepacia have been immobilized on octyl and cyanogen bromide (CNBr) agarose beads. The immobilization on octyl-agarose is slowed with increasing ionic strength, while the immobilization on CNBr is not significantly affected by the ionic strength. The inhibition o...

journal_title：Enzyme and microbial technology

authors： Manoel EA,Dos Santos JC,Freire DM,Rueda N,Fernandez-Lafuente R

更新日期：2015-04-01 00:00:00

abstract：:The selected thermotolerant, lactose-utilizing yeast strain Kluyveromyces marxianus NBIMCC 8362 possesses high specific d-amino acid oxidase activity (60Ug(-1)), which was increased nine-fold (545Ug(-1)) by design of the growth medium and conditions for d-amino oxidase induction. Applying an optimized simple and rapid...

journal_title：Enzyme and microbial technology

authors： Kostova DD,Petrova VY,Kujumdzieva AV

更新日期：2008-01-01 00:00:00

abstract：:Monod kinetic parameters provide information required for kinetic analysis of anaerobic oxidation of methane coupled to denitrification (AOM-D). This information is critical for engineering AOM-D processes in wastewater treatment facilities. We first experimentally determined Monod kinetic parameters for an AOM-D enri...

journal_title：Enzyme and microbial technology

authors： Yu H,Kashima H,Regan JM,Hussain A,Elbeshbishy E,Lee HS

更新日期：2017-09-01 00:00:00

abstract：:6-(N-hydroxyethyl)-amino-6-deoxy-l-sorbofuranose (6NSL), a key precursor in the synthesis of miglitol, is produced from N-2-hydroxyethyl-glucamine (NHEG) by the regioselective oxidation of Gluconobacter oxydans. The limitation of PQQ biosynthesis became a bottleneck for improvement of PQQ-dependent D-sorbitol dehydrog...

journal_title：Enzyme and microbial technology

authors： Liu D,Ke X,Hu ZC,Zheng YG

更新日期：2020-11-01 00:00:00

abstract：:In the previous study, glutathione production was elevated by adding precursor amino acids and adenosine triphosphate (ATP) in high cell density cultivation of Candida utilis. Furthermore, in the present research, glutathione production was further improved by optimizing sodium dedecyl sulfate (SDS) addition coupled w...

journal_title：Enzyme and microbial technology

authors： Liang G,Mo Y,Du G

更新日期：2010-05-05 00:00:00

abstract：:A novel endoglucanase active on 1,3-1,4-beta-D-glucans was purified to apparent homogeneity from submerged cultures of the moderately thermophilic aerobic fungus Talaromyces emersonii CBS 814.70. The enzyme is a single subunit glycoprotein with M(r) and pI values of 40.7 +/- 0.3 kDa and 4.4, respectively, and an estim...

journal_title：Enzyme and microbial technology

authors： Murray PG,Grassick A,Laffey CD,Cuffe MM,Higgins T,Savage AV,Planas A,Tuohy MG

更新日期：2001-07-05 00:00:00

abstract：:During toluene hydroxylation, catalyzed by Pseudomonas putida UV4 one molecule of oxygen is added to the aromatic ring to produce the dihydroxylated (non-aromatic) ring structure, toluene cis-glycol. Toluene, which is toxic to the cells at aqueous phase concentration above ( approximately 2.4 mmol), is fed to the reac...

journal_title：Enzyme and microbial technology

authors： Carragher JM,McClean WS,Woodley JM,Hack CJ

更新日期：2001-02-01 00:00:00

abstract：:We here describe a unique β-D-glucosidase (BGL; Blon_0625) derived from Bifidobacterium longum subsp. infantis ATCC 15697. The Blon_0625 gene was expressed by recombinant Escherichia coli. Purified recombinant Blon_0625 retains hydrolyzing activity against both p-nitrophenyl-β-D-glucopyranoside (pNPG; 17.3±0.24Umg(-1)...

journal_title：Enzyme and microbial technology

authors： Matsumoto T,Shimada S,Hata Y,Tanaka T,Kondo A

更新日期：2015-01-01 00:00:00

abstract：:A methyl parathion hydrolase (MPH) gene, bjmpd, was cloned from a newly isolated MP-degrading bacterial strain, Burkholderia jiangsuensis MP-1T and heterologously expressed in Escherichia coli BL21 (DE3). Although the amino acid sequence of the bjmpd-encoded enzyme, named BjMPH, differed from that of MPH from Pseudomo...

journal_title：Enzyme and microbial technology

authors： Liu XY,Chen FF,Li CX,Luo XJ,Chen Q,Bai YP,Xu JH

更新日期：2016-11-01 00:00:00

abstract：:This study reports a general strategy for the encapsulation of various enzymes in amphiphilic hollow carbonaceous microspheres (CMs). We found that enzymes could be spontaneously encapsulated in the interior cavity of the CMs via hydrophobic interactions. Due to strong hydrophobic interactions and robust confinement, ...

journal_title：Enzyme and microbial technology

authors： Liu L,Sun Y,He L,Jiang L,Yang S

更新日期：2015-11-01 00:00:00

abstract：:The novel termite gut metagenomic-derived GH11 xylanase gene xyl7 was expressed in Escherichia coli BL21, and the purified XYL7 enzyme exhibited high specific activity (6340U/mg) and broad pH active range of 5.5-10.0. Directed evolution was employed to enhance the thermostability of XYL7; two mutants (XYL7-TC and XYL7...

journal_title：Enzyme and microbial technology

authors： Qian C,Liu N,Yan X,Wang Q,Zhou Z,Wang Q

更新日期：2015-03-01 00:00:00