当前位置： SCI文献检索 > ENZYME AND MICROBIAL TECHNOLOGY期刊下所有文献 > Genome mining and motif truncation of glycoside hydrolase family 5 endo-β-1,4-mannanase encoded by Aspergillus oryzae RIB40 for potential konjac flour hydrolysis or feed additive.

Genome mining and motif truncation of glycoside hydrolase family 5 endo-β-1,4-mannanase encoded by Aspergillus oryzae RIB40 for potential konjac flour hydrolysis or feed additive.

Abstract：

:Two novel glycosyl hydrolase family 5 (GH5) β-mannanases (AoMan5A and AoMan5B) were identified from Aspergillus oryzae RIB40 by genome mining. The AoMan5A contains a predicted family 1 carbohydrate binding module (CBM-1), located at its N-terminal. The AoMan5A, AoMan5B and truncated mutant AoMan5AΔCL (truncating the N-terminal CBM and linker of AoMan5A) were expressed retaining the N-terminus of the native protein in Pichia pastoris GS115 by pPIC9KM. The specific enzyme activity of the purified reAoMan5A, reAoMan5B and reAoMan5AΔCL towards locust bean gum at pH 3.6 and 40°C for 10min, was 8.3, 104.2 and 15.8U/mg, respectively. The temperature properties of the reAoMan5AΔCL were improved by truncating CBM. They can degrade the pretreated konjac flour and produce prebiotics. In addition, they had excellent stability under simulative gastric fluid and simulative prilling process. All these properties make these recombinant β-mannanases potential additives for use in the food and feed industries.

journal_name

Enzyme Microb Technol

journal_title

Enzyme and microbial technology

authors

Tang CD,Shi HL,Tang QH,Zhou JS,Yao LG,Jiao ZJ,Kan YC

doi

10.1016/j.enzmictec.2016.08.003

subject

Has Abstract

pub_date

2016-11-01 00:00:00

pages

99-104

eissn

0141-0229

issn

1879-0909

pii

S0141-0229(16)30147-8

journal_volume

93-94

pub_type

杂志文章

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