Enzymatic synthesis of phenethyl ester from phenethyl alcohol with acyl donors.

abstract：:The objective of this study was to screen and identify α-amylase inhibitor peptides from Pinto bean. Five Pinto bean bioactive peptides were successfully identified: PPHMLP (P1), PLPWGAGF (P3), PPHMGGP (P6), PLPLHMLP (P7) and LSSLEMGSLGALFVCM (P9). Based on ELISA results, their promising optical density values were 1....

journal_title：Enzyme and microbial technology

authors： Ngoh YY,Lim TS,Gan CY

更新日期：2016-07-01 00:00:00

abstract：:We demonstrate the feasibility of growing crystals of protein in volumes as small as 1 nanoliter. Advances in the handling of very small volumes (i.e. through inkjet and other technologies) open the way towards fully automated systems. The rationale for these experiments is the desire to develop a system that speeds u...

journal_title：Enzyme and microbial technology

authors： Kuil ME,Bodenstaff ER,Hoedemaeker FJ,Abrahams JP

更新日期：2002-03-13 00:00:00

abstract：:By using very active and very stable penicillin G acylase (PGA)--agarose derivatives we have studied the industrial design of equilibrium-controlled synthesis of lactamic antibiotics. In the presence of high concentrations of organic cosolvents we have carried out the direct enzymatic condensation of phenylacetic acid...

journal_title：Enzyme and microbial technology

authors： Fernandez-Lafuente R,Rosell CM,Guisán JM

更新日期：1991-11-01 00:00:00

abstract：:Catalase was covalently immobilized onto florisil via glutaraldehyde (GA) and glutaraldehyde+6-amino hexanoic acid (6-AHA) (as a spacer arm). Immobilizations of catalase onto modified supports were optimized to improve the efficiency of the overall immobilization procedures. The V(max) values of catalase immobilized v...

journal_title：Enzyme and microbial technology

authors： Alptekin O,Tükel SS,Yildirim D,Alagöz D

更新日期：2011-12-10 00:00:00

abstract：:A reactive water-soluble polymer was synthesized by copolymerizing N-isopropylacrylamide and glycidyl acrylate. The reactive polymer could react with the amino groups of enzymes/proteins or other ligands to form an affinity polymer. As a model, the reactive polymer was allowed to react with paraaminobenzamidine, a str...

journal_title：Enzyme and microbial technology

authors： Nguyen AL,Luong JH

更新日期：1990-09-01 00:00:00

abstract：:We report a new colorimetric assay to quantify endo-polygalacturonase activity, which hydrolyzes polygalacturonic acid to produce smaller chains of galacturonate. Some of the reported polygalacturonase assays measure the activity by detecting the appearance of reducing ends such as the Somogyi-Nelson method. As a resu...

journal_title：Enzyme and microbial technology

authors： Torres S,Sayago JE,Ordoñez RM,Isla MI

更新日期：2011-02-08 00:00:00

abstract：:Based on a differential pre-screening of 44 white-rot fungi on a lignocellulose-supplemented minimal medium, four basidiomycetes were selected for further study: Ganoderma lucidum, Polyporus brumalis, Polyporus ciliatus and Trametes versicolor. Only G. lucidum was able to grow vividly on malt extract or minimal media ...

journal_title：Enzyme and microbial technology

authors： Sitarz AK,Mikkelsen JD,Højrup P,Meyer AS

更新日期：2013-12-10 00:00:00

abstract：:Addition of non-ionic surfactants (NIS) is known to accelerate enzymatic lignocellulose hydrolysis. The mechanism behind this accelerating effect is still not elucidated but has been hypothesized to originate from favorable NIS-lignin interactions which alleviate non-productive adsorption of cellulases to lignin. In t...

journal_title：Enzyme and microbial technology

authors： Olsen SN,Bohlin C,Murphy L,Borch K,McFarland KC,Sweeny MD,Westh P

更新日期：2011-09-10 00:00:00

abstract：:Peptide T has a sequence (Ala-Ser-Thr-Thr-Thr-Asn-Tyr-Thr) belonging to HIV envelope that is involved in the interaction with CD(4) receptor of T lymphocytes. Research of protease activities towards this peptide is very relevant for AIDS therapy. Characterization of specificity of subtilisin Carlsberg towards this ver...

journal_title：Enzyme and microbial technology

authors： Nedjar-Arroumea N,Paon M,Koralewski F,Friboulet A,Kouach M,Briand G,Leroy Y,Guillochon D

更新日期：2000-03-01 00:00:00

abstract：:The gene encoding a cold-adapted phospholipase A(1) (PLA(1)) from a psychrotrophic, glacier soil bacterium Serratia sp. xjF1 was cloned by two-step PCR (general PCR and TAIL-PCR). The full-length fragment comprised two open reading frames plA and plS. The gene product of plA encoding 320 amino acids with a molecular w...

journal_title：Enzyme and microbial technology

authors： Fu J,Huang H,Meng K,Yuan T,Yao B,Shi Y,Ouyang P

更新日期：2008-01-01 00:00:00

abstract：:Spodoptera frugiperda insect cells (IPLB-Sf21-AE) (Sf21), infected with baculovirus expression vectors during their exponential growth phase, are commonly used to produce a variety of heterologous recombinant proteins. In the present study the culture conditions of these insect cells were studied to establish high-den...

journal_title：Enzyme and microbial technology

authors： Deutschmann SM,Jäger V

更新日期：1994-06-01 00:00:00

abstract：:Global substitution of canonical amino acids (cAAs) with noncanonical (ncAAs) counterparts in proteins whose function is dependent on post-translational events such as cofactor binding is still a methodically challenging and difficult task as ncAA insertion generally interferes with the cofactor biosynthesis machinery...

journal_title：Enzyme and microbial technology

authors： Völler JS,Thi To TM,Biava H,Koksch B,Budisa N

更新日期：2017-11-01 00:00:00

abstract：:Biocatalytic peptide synthesis will benefit from enzymes that are active at low water levels in organic solvent compositions that allow good substrate and product solubility. To explore the use of proteases from thermophiles for peptide synthesis under such conditions, putative protease genes of the subtilase class we...

journal_title：Enzyme and microbial technology

authors： Toplak A,Nuijens T,Quaedflieg PJ,Wu B,Janssen DB

更新日期：2015-06-01 00:00:00

abstract：:We have examined the chain length selectivity for a series of acyl donors by lipase B from Candida antarctica (CalB). CalB accepted aliphatic diesters of C4, C6 and C12 chain lengths equally. The introduction of a carbon-carbon double bond into the C4 esters dramatically lowered the rate constant associated with polym...

journal_title：Enzyme and microbial technology

authors： Frampton MB,Zelisko PM

更新日期：2014-05-10 00:00:00

abstract：:This work describes for the first time the green synthesis of neopentyl glycol diheptanoate in a solvent-free medium via an enzymatic pathway. The process has been carried out in an open-air reactor in order to ease water removal through evaporation and shift the chemical equilibrium towards product formation. The inh...

journal_title：Enzyme and microbial technology

authors： Serrano-Arnaldos M,García-Martínez JJ,Ortega-Requena S,Bastida J,Máximo F,Montiel MC

更新日期：2020-01-01 00:00:00

abstract：:Effects of pulse and step changes in major metabolite concentrations such as glucose, glutamine, lactic acid, and ammonium on behavior of T-cell hybridomas in continuous culture were investigated in order to develop a strategy to maximize specific rate of lymphokine formation. Step increases in glucose, lactic acid, a...

journal_title：Enzyme and microbial technology

authors： Shaughnessy TS,Kargi F

更新日期：1990-09-01 00:00:00

abstract：:Developing a strain with high docosahexaenoic acid (DHA) yield and stable fermenting-performance is an imperative way to improve DHA production using Aurantiochytrium sp., a microorganism with two fatty acid synthesis pathways: polyketide synthase (PKS) pathway and Type I fatty acid synthase (FAS) pathway. This study ...

journal_title：Enzyme and microbial technology

authors： Cheng YR,Sun ZJ,Cui GZ,Song X,Cui Q

更新日期：2016-11-01 00:00:00

abstract：:Isolates from gardening waste compost and 38 culture collection microbes were grown on agar plates at pH 4.0 with the cutinase model substrate polycaprolactone as a carbon source. The strains showing polycaprolactone hydrolysis were cultivated in liquid at acidic pH and the cultivations were monitored by assaying the ...

journal_title：Enzyme and microbial technology

authors： Nyyssölä A,Pihlajaniemi V,Järvinen R,Mikander S,Kontkanen H,Kruus K,Kallio H,Buchert J

更新日期：2013-04-10 00:00:00

abstract：:A novel endoglucanase active on 1,3-1,4-beta-D-glucans was purified to apparent homogeneity from submerged cultures of the moderately thermophilic aerobic fungus Talaromyces emersonii CBS 814.70. The enzyme is a single subunit glycoprotein with M(r) and pI values of 40.7 +/- 0.3 kDa and 4.4, respectively, and an estim...

journal_title：Enzyme and microbial technology

authors： Murray PG,Grassick A,Laffey CD,Cuffe MM,Higgins T,Savage AV,Planas A,Tuohy MG

更新日期：2001-07-05 00:00:00

abstract：:An improved amperometric l-lactate biosensor was constructed based on covalent immobilization of lactate oxidase (LOx) from Pediococcus species onto carboxylated multiwalled carbon nanotubes (cMWCNT)/copper nanoparticles (CuNPs)/polyaniline (PANI) hybrid film electrodeposited on the surface of a pencil graphite electr...

journal_title：Enzyme and microbial technology

authors： Dagar K,Pundir CS

更新日期：2017-01-01 00:00:00

abstract：:Enzymatic production of cephalexin using immobilized penicillin G acylase was studied. The factors that affect the conversion of 7-amino-3-deacetoxycephalosporanic acid (7-ADCA) to cephalexin, such as pH; temperature; concentrations of 7-ADCA, D(-)-phenylglycine methyl ester (PGME), and immobilized penicillin G acylas...

journal_title：Enzyme and microbial technology

authors： Maladkar NK

更新日期：1994-08-01 00:00:00

abstract：:Ergosterol, like cholesterol, has many beneficial physiological activities, and because it has no adverse clinical problem with cholesterol, it is a substance that can be used as a biomaterial in the food and cosmetics industry. However, ergosterol has low oil solubility and is easily crystallized, which is problemati...

journal_title：Enzyme and microbial technology

authors： Park SH,Kim HK

更新日期：2020-09-01 00:00:00

abstract：:Laccases (benzenediol oxygen oxidoreductases, EC 1.10.3.2) are important multicopper enzymes that are used in many biotechnological processes. A recombinant form of laccase from Bacillus sp. HR03 was overexpressed in Escherichia coli BL-21(DE3). Inclusion body (IB) formation happens quite often during recombinant prot...

journal_title：Enzyme and microbial technology

authors： Mollania N,Khajeh K,Ranjbar B,Rashno F,Akbari N,Fathi-Roudsari M

更新日期：2013-05-10 00:00:00

abstract：:Zygosaccharomyces rouxii is an important yeast in the formation of flavor in soy sauce. In this study, we investigated the separate effects of exogenous threonine, cystathionine, and the branched-chain amino acids on the metabolism of Z. rouxii. The addition of these amino acids had significant effects on both Z. roux...

journal_title：Enzyme and microbial technology

authors： van der Sluis C,Wolken WA,Giuseppin ML,Tramper J,Wijffels RH

更新日期：2000-02-01 00:00:00

abstract：:To increase the efficiency of enzyme cocktails in deconstructing cellulose and hemicelluloses present in the plant cell wall, a combination of enzymes with complementary activities is required. Xylan is the main hemicellulose component of energy crops and for its complete hydrolysis a system consisting of several enzy...

journal_title：Enzyme and microbial technology

authors： Cintra LC,da Costa IC,de Oliveira ICM,Fernandes AG,Faria SP,Jesuíno RSA,Ravanal MC,Eyzaguirre J,Ramos LP,de Faria FP,Ulhoa CJ

更新日期：2020-02-01 00:00:00

abstract：:Direct cellobiose production from cellulose by a genetically modified fungus-Neurospora crassa, was explored in this study. A library of N. crassa sextuple beta-glucosidase (bgl) gene deletion strains was constructed. Various concentrations of cellobiose were detected in the culture broth of the N. crassa sextuple bet...

journal_title：Enzyme and microbial technology

authors： Wu W,Hildebrand A,Kasuga T,Xiong X,Fan Z

更新日期：2013-03-05 00:00:00

abstract：:Recently enzymatic catalysts have replaced organic and organometallic catalysts in the synthesis of bio-resorbable polymers. Enzymatic polymerization is considered as an alternative to conventional polymerization as they are less toxic, environmental friendly and can operate under mild conditions. In this research, th...

journal_title：Enzyme and microbial technology

authors： Pakalapati H,Tariq MA,Arumugasamy SK

更新日期：2019-03-01 00:00:00

abstract：:The effect of intense pulsed light (IPL) irradiation on Chromobacterium viscosum lipase was investigated with a primary focus on catalytic activity and molecular structure. During IPL irradiation, lipase activity decreased significantly with increasing pulse fluence (Fp) and exposure time (te). IPL-induced deactivatio...

journal_title：Enzyme and microbial technology

authors： Jeon MS,Park KM,Yu H,Park JY,Chang PS

更新日期：2019-05-01 00:00:00

abstract：:Gamma glutamyl transpeptidase from Bacillus pumilus KS12 (GGTBP) was cloned, expressed in pET-28-E. coli expression system as a heterodimeric enzyme with molecular weights of 45 and 20 kDa for large and small subunit, respectively. It was purified by nickel affinity chromatography with hydrolytic and transpeptidase ac...

journal_title：Enzyme and microbial technology

authors： Murty NA,Tiwary E,Sharma R,Nair N,Gupta R

更新日期：2012-03-10 00:00:00

abstract：:Kinetic resolution of sec-alcohols is a green process with biocatalyst. Candida antarctica lipase B (CALB) displayed on Pichia pastoris cell-surface (Pp-CALB) was characterized in kinetic resolution of sec-alcohols with different structures. The reaction parameters including acyl donors, molar ratio of substrates, sol...

journal_title：Enzyme and microbial technology

authors： Zhang K,Pan Z,Diao Z,Liang S,Han S,Zheng S,Lin Y

更新日期：2018-03-01 00:00:00