Effect of threonine, cystathionine, and the branched-chain amino acids on the metabolism of Zygosaccharomyces rouxii*

abstract：:This review focuses on the description of recently discovered esterase enzymes involved in xylan degradation (acetyl xylan, feruloyl, and p-coumaroyl esterases). The occurrence of these enzymes in various microorganisms, assays used for determination of their activity, induction and production on different substrates,...

journal_title：Enzyme and microbial technology

authors： Christov LP,Prior BA

更新日期：1993-06-01 00:00:00

abstract：:In the present investigation, the bacterium Escherichia coli (VM1) was isolated from a marine sediments of Ennore coastal water along southeast coast of India was exploited for its capability of Ag NPs synthesis. The derived nanoparticles were characterized employing ultraviolet-visible (UV-vis) spectroscopy, Fourier ...

journal_title：Enzyme and microbial technology

authors： Maharani V,Sundaramanickam A,Balasubramanian T

更新日期：2016-12-01 00:00:00

abstract：:Solid-liquid enzyme reactions constitute important processes in biochemical industries. The isomerization of d-glucose to d-fructose, using the immobilized glucose isomerase (Sweetzyme T), as a typical example of solid-liquid catalyzed reactions has been carried out in one stage and multi-stage novel type of impinging...

journal_title：Enzyme and microbial technology

authors： Fatourehchi N,Sohrabi M,Dabir B,Royaee SJ,Haji Malayeri A

更新日期：2014-02-05 00:00:00

abstract：:The cell wall integrity and stress response component (WSC) domain was first described in the Wsc-family protein of the yeast Saccharomyces cerevisiae, and later found in diverse eukaryotic organisms. Due solely to their presence in the Wsc-family proteins working as a plasma membrane sensor for surface stress and in ...

journal_title：Enzyme and microbial technology

authors： Oide S,Tanaka Y,Watanabe A,Inui M

更新日期：2019-06-01 00:00:00

abstract：:Two GH43 β-xylosidases, RS223-BX from a rice straw metagenomic library, and BoXA from Bacteroides ovatus, that share similar amino acid sequences (81% identical) and 19 of 20 active-site residues, were compared by using site-directed mutagenesis of Asp and His residues implicated in metal binding. Thus, RS223-BX is st...

journal_title：Enzyme and microbial technology

authors： Jordan DB,Stoller JR,Kibblewhite RE,Chan VJ,Lee CC,Wagschal K

更新日期：2018-07-01 00:00:00

abstract：:Gamma glutamyl transpeptidase from Bacillus pumilus KS12 (GGTBP) was cloned, expressed in pET-28-E. coli expression system as a heterodimeric enzyme with molecular weights of 45 and 20 kDa for large and small subunit, respectively. It was purified by nickel affinity chromatography with hydrolytic and transpeptidase ac...

journal_title：Enzyme and microbial technology

authors： Murty NA,Tiwary E,Sharma R,Nair N,Gupta R

更新日期：2012-03-10 00:00:00

abstract：:By using very active and very stable penicillin G acylase (PGA)--agarose derivatives we have studied the industrial design of equilibrium-controlled synthesis of lactamic antibiotics. In the presence of high concentrations of organic cosolvents we have carried out the direct enzymatic condensation of phenylacetic acid...

journal_title：Enzyme and microbial technology

authors： Fernandez-Lafuente R,Rosell CM,Guisán JM

更新日期：1991-11-01 00:00:00

abstract：:Developing a strain with high docosahexaenoic acid (DHA) yield and stable fermenting-performance is an imperative way to improve DHA production using Aurantiochytrium sp., a microorganism with two fatty acid synthesis pathways: polyketide synthase (PKS) pathway and Type I fatty acid synthase (FAS) pathway. This study ...

journal_title：Enzyme and microbial technology

authors： Cheng YR,Sun ZJ,Cui GZ,Song X,Cui Q

更新日期：2016-11-01 00:00:00

abstract：:Conventional lipase screening methods are mostly based on hydrolytic activity, which may not always be the best method to assess the enzyme activity, especially for evaluating synthetic activity. Here we developed a high throughput and visual method to screen clones with high synthetic activity and used it to assess l...

journal_title：Enzyme and microbial technology

authors： Zhang JH,Lin Y,Sun YF,Ye YR,Zheng SP,Han SY

更新日期：2012-05-10 00:00:00

abstract：:The substrate specificities of alpha-chymotrypsin and subtilisins for peptide synthesis in hydrophilic organic solvents were investigated. Chymotrypsin exhibited high specificity to aromatic amino acids as acyl donors, while subtilisin Carlsberg and subtilisin BPN' were specific to aromatic and neutral aliphatic amino...

journal_title：Enzyme and microbial technology

authors： Nagashima T,Watanabe A,Kise H

更新日期：1992-10-01 00:00:00

abstract：:During toluene hydroxylation, catalyzed by Pseudomonas putida UV4 one molecule of oxygen is added to the aromatic ring to produce the dihydroxylated (non-aromatic) ring structure, toluene cis-glycol. Toluene, which is toxic to the cells at aqueous phase concentration above ( approximately 2.4 mmol), is fed to the reac...

journal_title：Enzyme and microbial technology

authors： Carragher JM,McClean WS,Woodley JM,Hack CJ

更新日期：2001-02-01 00:00:00

abstract：:Bacterial chitinases recruited multiple accessory domains for the conversion of recalcitrant polysaccharides to simple soluble sugars/amino sugars. Here, we report detailed properties of a multi-domain GH18 chitinase from Enterobacter cloacae subsp. cloacae (EcChi2) that preferred β-chitin as substrate. EcChi2 exhibit...

journal_title：Enzyme and microbial technology

authors： Mallakuntla MK,Podile AR

更新日期：2021-02-01 00:00:00

abstract：:GH11 xylanase XynJ from Bacillus sp. strain 41M-1 has a β-jellyroll fold composed of eight β strands with a deep active-site cleft. We hypothesized that the thermostability of XynJ will increase if the flexibility of the β strands in the jellyroll structure is decreased without impairing activity. To verify this hypot...

journal_title：Enzyme and microbial technology

authors： Takita T,Nakatani K,Katano Y,Suzuki M,Kojima K,Saka N,Mikami B,Yatsunami R,Nakamura S,Yasukawa K

更新日期：2019-11-01 00:00:00

abstract：:Nine proteins with lignin peroxidase activity were separated from cultures of Phanerochaete chrysosporium INA-12 in glycerol as carbon source and non-nitrogen limited. Four lignin peroxidase isozymes (4, 5, 8, 9) were purified and characterized. Although differences in kinetic parameters could be shown, antibody react...

journal_title：Enzyme and microbial technology

authors： Odier E,Delattre M

更新日期：1990-06-01 00:00:00

abstract：:By using very active and very stable trypsin agarose derivatives, we have optimized the design of the synthesis of a model dipeptide, benzoylarginine leucinamide, by two different strategies: (i) kinetically controlled synthesis (KCS), by using benzoyl arginine ethyl ester and leucinamide as substrates, and (ii) therm...

journal_title：Enzyme and microbial technology

authors： Blanco RM,Alvaro G,Guisán JM

更新日期：1991-07-01 00:00:00

abstract：:Escherichia coli B/pTG201 recombinant cells were immobilized by entrapment in a carrageenan gel and cultivated in nonselective media to investigate the effect of agitation rate on plasmid stability, biomass concentration, and enzyme productivity. These parameters were studied in continuous cultures for free and immobi...

journal_title：Enzyme and microbial technology

authors： Huang J,Dhulster P,Thomas D,Barbotin JN

更新日期：1990-12-01 00:00:00

abstract：:Bacterial spores maintain metabolic dormancy and have high resistance to external pressure. Germination requires degradation of the spore cortex and the participation of germination-specific cortex-lytic enzymes (GSLEs). Previously reported GSLEs have been identified in bacteria and facilitate germination. In this stu...

journal_title：Enzyme and microbial technology

authors： Fu Y,Liang L,Deng S,Wu Y,Yuan Y,Gao M

更新日期：2020-12-01 00:00:00

abstract：:In the present work, NAD(+)-dependent formate dehydrogenase (FDH), encoded by fdh gene from Candida boidinii was successfully displayed on Escherichia coli cell surface using ice nucleation protein (INP) from Pseudomonas borealis DL7 as an anchoring protein. Localization of matlose binding protein (MBP)-INP-FDH fusion...

journal_title：Enzyme and microbial technology

authors： Liu A,Feng R,Liang B

更新日期：2016-09-01 00:00:00

abstract：:The alkane-1-monoxygenase (alkB) complex of Pseudomonas putida GPo1 has been extensively studied in the past and shown to be capable of oxidising aliphatic C(5)-C(12) alkanes to primary alcohols both in the wild-type organism by growth on C(5)-C(12) alkanes as sole carbon source and in vitro. Despite this, successful ...

journal_title：Enzyme and microbial technology

authors： Grant C,Woodley JM,Baganz F

更新日期：2011-05-06 00:00:00

abstract：:Enzymatic production of cephalexin using immobilized penicillin G acylase was studied. The factors that affect the conversion of 7-amino-3-deacetoxycephalosporanic acid (7-ADCA) to cephalexin, such as pH; temperature; concentrations of 7-ADCA, D(-)-phenylglycine methyl ester (PGME), and immobilized penicillin G acylas...

journal_title：Enzyme and microbial technology

authors： Maladkar NK

更新日期：1994-08-01 00:00:00

abstract：:Recently enzymatic catalysts have replaced organic and organometallic catalysts in the synthesis of bio-resorbable polymers. Enzymatic polymerization is considered as an alternative to conventional polymerization as they are less toxic, environmental friendly and can operate under mild conditions. In this research, th...

journal_title：Enzyme and microbial technology

authors： Pakalapati H,Tariq MA,Arumugasamy SK

更新日期：2019-03-01 00:00:00

abstract：:The activities (at pH 7 and 50 degrees C) of purified EGV (Humicola insolens) and CenA (Cellulomonas fimi) were determined on cotton fabrics at high and low levels of mechanical agitation. Similar activity measurements were also made by using the core domains of these cellulases. Activity experiments suggested that th...

journal_title：Enzyme and microbial technology

authors： Azevedo H,Bishop D,Cavaco-Paulo A

更新日期：2000-08-01 00:00:00

abstract：:A methyl parathion hydrolase (MPH) gene, bjmpd, was cloned from a newly isolated MP-degrading bacterial strain, Burkholderia jiangsuensis MP-1T and heterologously expressed in Escherichia coli BL21 (DE3). Although the amino acid sequence of the bjmpd-encoded enzyme, named BjMPH, differed from that of MPH from Pseudomo...

journal_title：Enzyme and microbial technology

authors： Liu XY,Chen FF,Li CX,Luo XJ,Chen Q,Bai YP,Xu JH

更新日期：2016-11-01 00:00:00

abstract：:The heat and mass transfer have been proved to be the important factors in air pressure pulsation for cellulase production. However, as process of enzyme secretion, the cellulase formation has not been studied in the view of microorganism metabolism and metabolic key enzyme activity under air pressure pulsation condit...

journal_title：Enzyme and microbial technology

authors： Chen H,Shao M,Li H

更新日期：2014-03-05 00:00:00

abstract：:Continuous culture and fed-batch fermentations were used to test the behavior of the system Bacillus subtilis DN1885(pCH7) that synthesizes a recombinant beta-1,4-endoglucanase. Continuous culture experiments were focused on the study of the instability aspects of the system as well as determination of the biomass gro...

journal_title：Enzyme and microbial technology

authors： Shene C,Mir N,Andrews BA,Asenjo JA

更新日期：2000-08-01 00:00:00

abstract：:A method to quantify the impact of proteolysis on accumulation of recombinant proteins in E. coli is described. A much smaller intracellular concentration of staphylococcal protein A (SpA) (14.7 mg. g(-1)) compared to the fusion protein SpA-betagalactosidase (138 mg. g(-1)) is explained by a very high proteolysis rate...

journal_title：Enzyme and microbial technology

authors： Rozkov A,Schweder T,Veide A,Enfors S

更新日期：2000-12-01 00:00:00

abstract：:Before applying nanotechnologies in biomedical and environmental areas it is advised to study interactions of nanoparticles and other nanomaterials with biomacromolecule present in living system. Moreover there is scarcity of reports on interactions between nanoparticles and biomaterials. In present report a rapid, ec...

journal_title：Enzyme and microbial technology

authors： Patil CD,Borase HP,Suryawanshi RK,Patil SV

更新日期：2016-10-01 00:00:00

abstract：:To increase the efficiency of enzyme cocktails in deconstructing cellulose and hemicelluloses present in the plant cell wall, a combination of enzymes with complementary activities is required. Xylan is the main hemicellulose component of energy crops and for its complete hydrolysis a system consisting of several enzy...

journal_title：Enzyme and microbial technology

authors： Cintra LC,da Costa IC,de Oliveira ICM,Fernandes AG,Faria SP,Jesuíno RSA,Ravanal MC,Eyzaguirre J,Ramos LP,de Faria FP,Ulhoa CJ

更新日期：2020-02-01 00:00:00

abstract：:Isolates from gardening waste compost and 38 culture collection microbes were grown on agar plates at pH 4.0 with the cutinase model substrate polycaprolactone as a carbon source. The strains showing polycaprolactone hydrolysis were cultivated in liquid at acidic pH and the cultivations were monitored by assaying the ...

journal_title：Enzyme and microbial technology

authors： Nyyssölä A,Pihlajaniemi V,Järvinen R,Mikander S,Kontkanen H,Kruus K,Kallio H,Buchert J

更新日期：2013-04-10 00:00:00

abstract：:An analysis developed by Westgate et al. for the digestible energy of edible and inedible biomass, including hydrolysis and fermentation, is reexamined with state-of-the-art values for the harvest index of hydroponic crops. ...

journal_title：Enzyme and microbial technology

authors： Volk T

更新日期：1993-10-01 00:00:00