Esterases of xylan-degrading microorganisms: production, properties, and significance.

abstract：:Sialic acid aldolases (E.C.4.1.3.3) catalyze the reversible aldol cleavage of N-acetyl-d-neuraminic acid (Neu5Ac) to from N-acetyl-d-mannosamine (ManNAc) and pyruvate. In this study, a sialic acid aldolase (PdNAL) from Peptoclostridium difficile NAP08 was expressed in Escherichia coli BL21 (DE3). This homotetrameric e...

journal_title：Enzyme and microbial technology

authors： Chen Q,Han L,Chen X,Cui Y,Feng J,Wu Q,Zhu D

更新日期：2016-10-01 00:00:00

abstract：:Enzymatic production of cephalexin using immobilized penicillin G acylase was studied. The factors that affect the conversion of 7-amino-3-deacetoxycephalosporanic acid (7-ADCA) to cephalexin, such as pH; temperature; concentrations of 7-ADCA, D(-)-phenylglycine methyl ester (PGME), and immobilized penicillin G acylas...

journal_title：Enzyme and microbial technology

authors： Maladkar NK

更新日期：1994-08-01 00:00:00

abstract：:A colorimetric platform based on enzyme-inorganic hybrid nanoflowers was used for ultraviolet-visible detection of hydrogen peroxide (H2O2). The hybrid nanoflowers were prepared via incubation at room temperature using horseradish peroxidase (HRP) and copper phosphate (Cu3(PO4)2) as the organic and inorganic component...

journal_title：Enzyme and microbial technology

authors： Yang C,Zhang M,Wang W,Wang Y,Tang J

更新日期：2020-10-01 00:00:00

abstract：:Musky macrolactones are an important group of compounds used in high-valued perfumery. An enzymatic cascade reaction including cytochrome P450 hydroxylase and lipase was explored to biosynthesize musky macrolactones. Firstly, fatty acids were hydroxylated by P450 hydroxylase to produce the corresponding ω-hydroxy fatt...

journal_title：Enzyme and microbial technology

authors： Meng S,Guo J,Li Z,Nie K,Xu H,Tan T,Liu L

更新日期：2019-12-01 00:00:00

abstract：:The parameters of biosensors based on the yeast strains Candida maltosa VKM Y-2359, Candida blankii VKM Y-2675, and Debaryomyces hansenii VKM Y-2482 for biochemical oxygen demand (BOD) detection are compared. The catalytic activity of the strains was analyzed in relation to the growth phase. The possibility of using D...

journal_title：Enzyme and microbial technology

authors： Arlyapov V,Kamanin S,Ponamoreva O,Reshetilov A

更新日期：2012-04-05 00:00:00

abstract：:The present study investigated the influence of low-shear modeled microgravity (LSMMG) conditions on Listeria monocytogenes stress response (heat, cold, and acid), membrane fatty acid composition, and virulence potential as well as stress-/virulence-associated gene expression. The results showed that LSMMG-cultivated ...

journal_title：Enzyme and microbial technology

authors： Sheet S,Yesupatham S,Ghosh K,Choi MS,Shim KS,Lee YS

更新日期：2020-02-01 00:00:00

abstract：:Kinetics of nitrification and denitrification of synthetic wastewater was investigated by using two reactors in series. An activated sludge unit was used for nitrification followed by a downflow biofilter for denitrification. Glucose solution was fed to the denitrification column to supply carbon source. Reactors were...

journal_title：Enzyme and microbial technology

authors： Dinçer AR,Kargi F

更新日期：2000-07-01 00:00:00

abstract：:A method to stably immobilize microbes on electrodes was developed. Resting cells of Methylobacterium extorquens AM1(MeAM1) were caged within multiwalled carbon nanotubes (MWNTs)by adding the cells to a water dispersion of MWNTs then allowing the resulting mixture to dry on electrodes. The MeAM1-MWCNTs electrode thus ...

journal_title：Enzyme and microbial technology

authors： Xia HQ,Sakai K,Kitazumi Y,Shirai O,Takagi K,Kano K

更新日期：2018-10-01 00:00:00

abstract：:This work describes for the first time the green synthesis of neopentyl glycol diheptanoate in a solvent-free medium via an enzymatic pathway. The process has been carried out in an open-air reactor in order to ease water removal through evaporation and shift the chemical equilibrium towards product formation. The inh...

journal_title：Enzyme and microbial technology

authors： Serrano-Arnaldos M,García-Martínez JJ,Ortega-Requena S,Bastida J,Máximo F,Montiel MC

更新日期：2020-01-01 00:00:00

abstract：:We report a new colorimetric assay to quantify endo-polygalacturonase activity, which hydrolyzes polygalacturonic acid to produce smaller chains of galacturonate. Some of the reported polygalacturonase assays measure the activity by detecting the appearance of reducing ends such as the Somogyi-Nelson method. As a resu...

journal_title：Enzyme and microbial technology

authors： Torres S,Sayago JE,Ordoñez RM,Isla MI

更新日期：2011-02-08 00:00:00

abstract：:Phenylalanine ammonia-lyase (PAL) gene plays an important role in the synthesis of flavones, lignin, and other bioactive compounds in living organisms. Inonotus baumii, the only known flavone-producing filamentous fungus, is of great importance in the investigation of flavone metabolic pathways. To study the function ...

journal_title：Enzyme and microbial technology

authors： Lin W,Liu A,Weng C,Li H,Sun S,Song A,Zhu H

更新日期：2018-05-01 00:00:00

abstract：:The different physical and chemical properties of lignin might have various effects on the enzymatic hydrolysis of lignocellulosic substrates. In this study, the influence of lignin on enzymatic digestibility of cellulose was assessed. Addition of 20% (4g/L) isolated enzymatic lignin (lignin 2 and 3) and kraft lignin ...

journal_title：Enzyme and microbial technology

authors： Zhang H,Wu S,Xie J

更新日期：2017-06-01 00:00:00

abstract：:Phenethyl ester is an important contributors to food, drinks, cosmetics and perfumery. Phenethyl acetate was synthesized enzymatically from phenethyl alcohol with acyl donors catalyzed by Novozym 435 from Candida antarctica lipase B. Through acyl donor screening, acetic anhydride and vinyl acetate were found to be the...

journal_title：Enzyme and microbial technology

authors： Kim H,Park C

更新日期：2017-05-01 00:00:00

abstract：:NADPH is the key cofactor in L-isoleucine (Ile) biosynthetic pathway. To increase the Ile biosynthesis in Corynebacterium glutamicum ssp. lactofermentum JHI3-156, NADPH supply needs to be enhanced. Here NAD kinase, the key enzyme for the de novo biosynthesis of NADP(+) and NADPH, were cloned and expressed in JHI3-156,...

journal_title：Enzyme and microbial technology

authors： Shi F,Huan X,Wang X,Ning J

更新日期：2012-07-15 00:00:00

abstract：:Growth conditions for mass production of luciferase-bacterial magnetic particles (BMPs) by a recombinant Magnetospirillum magneticum AMB-1 were investigated in a pH-regulated fed-batch culture system. Enrichment of growth medium with L-cysteine, yeast extract and polypeptone enhanced both bacterial growth and BMP prod...

journal_title：Enzyme and microbial technology

authors： Yang C,Takeyama H,Tanaka T,Matsunaga T

更新日期：2001-07-05 00:00:00

abstract：:Effects of different microaerophilic conditions on cell growth, glucose consumption, hydrogen production and cellular metabolism of wild Enterobacter aerogenes strain and polyphosphate kinase (PPK) overexpressing strain were systematically studied in this paper, using NaH(2)PO(4) as the phosphate sources. Under differ...

journal_title：Enzyme and microbial technology

authors： Lu Y,Zhang C,Lai Q,Zhao H,Xing XH

更新日期：2011-02-08 00:00:00

abstract：:l-ribose and d-tagatose are biochemically synthesized using sugar isomerases. The l-arabinose isomerase gene from Shigella flexneri (Sf-AI) was cloned and expressed in Escherichia coli BL-21. Sf-AI was applied for the bioproduction of d-tagatose from d-galactose. l-ribose synthesis was performed by two step isomerizat...

journal_title：Enzyme and microbial technology

authors： Patel MJ,Akhani RC,Patel AT,Dedania SR,Patel DH

更新日期：2017-02-01 00:00:00

abstract：:The fatty acid methyl esters and fatty acid ethyl esters are known as biodiesels which are considered to be renewable, nontoxic and biodegradable biofuels. However, the conventional biodiesels show a high crystallization temperature which is one of the most critical obstacles against the widespread biodiesel usage. Th...

journal_title：Enzyme and microbial technology

authors： Pan H,Zhang L,Li X,Guo D

更新日期：2017-07-01 00:00:00

abstract：:In this work, a biosensor using a glassy carbon electrode modified with gold nanoparticles (AuNPs) and tyrosinase (Tyr) within a dihexadecylphosphate film is proposed. Cystamine and glutaraldehyde crosslinking agents were used as a support for Tyr immobilization. The proposed biosensor was characterized by scanning el...

journal_title：Enzyme and microbial technology

authors： Campanhã Vicentini F,Garcia LL,Figueiredo-Filho LC,Janegitz BC,Fatibello-Filho O

更新日期：2016-03-01 00:00:00

abstract：:Developing a strain with high docosahexaenoic acid (DHA) yield and stable fermenting-performance is an imperative way to improve DHA production using Aurantiochytrium sp., a microorganism with two fatty acid synthesis pathways: polyketide synthase (PKS) pathway and Type I fatty acid synthase (FAS) pathway. This study ...

journal_title：Enzyme and microbial technology

authors： Cheng YR,Sun ZJ,Cui GZ,Song X,Cui Q

更新日期：2016-11-01 00:00:00

abstract：:3-Mercaptopyruvate (3MPy), a structural analog of 3-mercaptopropionic acid, is a precursor compound for biosynthesis of polythioesters in bacteria. The cost-effectiveness and sustainability of the whole process could be greatly improved by using the cysteine degradation pathway for an intracellular supply of 3MPy. Tra...

journal_title：Enzyme and microbial technology

authors： Andreeßen C,Gerlt V,Steinbüchel A

更新日期：2017-04-01 00:00:00

abstract：:The stabilization of fungal cellulases by spray drying, the thermal stability of Penicillium occitanis cellulases and the effect of some additives were studied. We observed that CMCase activity presents a good stability at 50 degrees C, even after 60 h of incubation. On the other hand, beta-glucosidase activity was mo...

journal_title：Enzyme and microbial technology

authors： Belghith H,Ellouz Chaabouni S,Gargouri A

更新日期：2001-02-01 00:00:00

abstract：:Continuous culture and fed-batch fermentations were used to test the behavior of the system Bacillus subtilis DN1885(pCH7) that synthesizes a recombinant beta-1,4-endoglucanase. Continuous culture experiments were focused on the study of the instability aspects of the system as well as determination of the biomass gro...

journal_title：Enzyme and microbial technology

authors： Shene C,Mir N,Andrews BA,Asenjo JA

更新日期：2000-08-01 00:00:00

abstract：:Corynebacterium glutamicum is an important industrial organism for the production of a variety of biological commodities. We discovered a promoter encoded by the gene NCgl2319 in C. glutamicum, which could be induced by benzyl alcohol, could be used as an efficient tunable expression system. In initial attempts, this ...

journal_title：Enzyme and microbial technology

authors： Liu X,Zhao Z,Dong G,Li Y,Peng F,Liu C,Zhang F,Linhardt RJ,Yang Y,Bai Z

更新日期：2020-11-01 00:00:00

abstract：:Biosensors have been extensively studied during the last 20 years, and a myriad of laboratory biosensors have been developed. Improvements are required in biosensor design and performance before they become widely accepted in industrial process monitoring. However, as the biotechnology industry expands, biosensors may...

journal_title：Enzyme and microbial technology

authors： Brooks SL,Higgins IJ,Newman JD,Turner AP

更新日期：1991-12-01 00:00:00

abstract：:An analysis developed by Westgate et al. for the digestible energy of edible and inedible biomass, including hydrolysis and fermentation, is reexamined with state-of-the-art values for the harvest index of hydroponic crops. ...

journal_title：Enzyme and microbial technology

authors： Volk T

更新日期：1993-10-01 00:00:00

abstract：:Operational stability under high temperature is required for enzyme application in industrial processes. Error-prone PCR and B-factor analysis were employed to enhance the thermostability of a xylanase from GH family 11 in this study. Based on the top 10 mutants screened from the random mutation libraries, mutant Xyn3...

journal_title：Enzyme and microbial technology

authors： Xing H,Zou G,Liu C,Chai S,Yan X,Li X,Liu R,Yang Y,Zhou Z

更新日期：2021-02-01 00:00:00

abstract：:Biocatalytic peptide synthesis will benefit from enzymes that are active at low water levels in organic solvent compositions that allow good substrate and product solubility. To explore the use of proteases from thermophiles for peptide synthesis under such conditions, putative protease genes of the subtilase class we...

journal_title：Enzyme and microbial technology

authors： Toplak A,Nuijens T,Quaedflieg PJ,Wu B,Janssen DB

更新日期：2015-06-01 00:00:00

abstract：:Horseradish peroxidase (HRP) was successfully purified from horseradish roots by a two-stage reverse-micellar extraction from the dialyzed aqueous extract. The anionic surfactant AOT dissolved in isooctane was used to produce the reverse-micellar phases. The narrow pH range at which HRP solubilization occurred was exp...

journal_title：Enzyme and microbial technology

authors： Regalado C,Asenjo JA,Pyle DL

更新日期：1996-04-01 00:00:00

abstract：:A method to quantify the impact of proteolysis on accumulation of recombinant proteins in E. coli is described. A much smaller intracellular concentration of staphylococcal protein A (SpA) (14.7 mg. g(-1)) compared to the fusion protein SpA-betagalactosidase (138 mg. g(-1)) is explained by a very high proteolysis rate...

journal_title：Enzyme and microbial technology

authors： Rozkov A,Schweder T,Veide A,Enfors S

更新日期：2000-12-01 00:00:00