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Optimization of sodium dedecyl sulfate (SDS) addition coupled with adenosine triphosphate (ATP) regeneration for glutathione overproduction in high density cultivation of Candida utilis.

Abstract：

:In the previous study, glutathione production was elevated by adding precursor amino acids and adenosine triphosphate (ATP) in high cell density cultivation of Candida utilis. Furthermore, in the present research, glutathione production was further improved by optimizing sodium dedecyl sulfate (SDS) addition coupled with ATP regeneration. Results indicated that with 2g/l ATP added at 60h and 0.8g/l SDS added repeatedly at 60, 63, 66 and 69h, final glutathione yield reached 2286mg/l after 72h cultivation. Moreover, by applying the novel strategies of regenerating ATP by feeding glucose at 6g/(lh) from 60 to 72h coupled with impulse SDS treatments, a total glutathione yield of 2485mg/l was achieved at 72h, which was 8.7% higher than with addition of ATP and SDS, suggesting application of the proposed strategies as being feasible for glutathione overproduction on industrial scales.

journal_name

Enzyme Microb Technol

journal_title

Enzyme and microbial technology

authors

Liang G,Mo Y,Du G

doi

10.1016/j.enzmictec.2010.02.007

subject

Has Abstract

pub_date

2010-05-05 00:00:00

pages

526-33

issue

6

eissn

0141-0229

issn

1879-0909

pii

S0141-0229(10)00040-2

journal_volume

46

pub_type

杂志文章

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