An efficient purification process for sweet potato beta-amylase by affinity precipitation with alginate.

abstract：:Musky macrolactones are an important group of compounds used in high-valued perfumery. An enzymatic cascade reaction including cytochrome P450 hydroxylase and lipase was explored to biosynthesize musky macrolactones. Firstly, fatty acids were hydroxylated by P450 hydroxylase to produce the corresponding ω-hydroxy fatt...

journal_title：Enzyme and microbial technology

authors： Meng S,Guo J,Li Z,Nie K,Xu H,Tan T,Liu L

更新日期：2019-12-01 00:00:00

abstract：:Based on a differential pre-screening of 44 white-rot fungi on a lignocellulose-supplemented minimal medium, four basidiomycetes were selected for further study: Ganoderma lucidum, Polyporus brumalis, Polyporus ciliatus and Trametes versicolor. Only G. lucidum was able to grow vividly on malt extract or minimal media ...

journal_title：Enzyme and microbial technology

authors： Sitarz AK,Mikkelsen JD,Højrup P,Meyer AS

更新日期：2013-12-10 00:00:00

abstract：:An analysis developed by Westgate et al. for the digestible energy of edible and inedible biomass, including hydrolysis and fermentation, is reexamined with state-of-the-art values for the harvest index of hydroponic crops. ...

journal_title：Enzyme and microbial technology

authors： Volk T

更新日期：1993-10-01 00:00:00

abstract：:A method to quantify the impact of proteolysis on accumulation of recombinant proteins in E. coli is described. A much smaller intracellular concentration of staphylococcal protein A (SpA) (14.7 mg. g(-1)) compared to the fusion protein SpA-betagalactosidase (138 mg. g(-1)) is explained by a very high proteolysis rate...

journal_title：Enzyme and microbial technology

authors： Rozkov A,Schweder T,Veide A,Enfors S

更新日期：2000-12-01 00:00:00

abstract：:Advantages in maltose fermentation at extremely low temperatures (5-10°C) using an alcohol resistant and cryotolerant yeast strain (Saccharomyces cerevisiae AXAZ-1) immobilized on porous cellulosic material (or tubular cellulose, TC), produced by delignification of wood sawdust, are reported. Pure maltose and glucose ...

journal_title：Enzyme and microbial technology

authors： Ganatsios V,Koutinas AA,Bekatorou A,Kanellaki M,Nigam P

更新日期：2014-11-01 00:00:00

abstract：:Various recombinant proteins can be produced by the yeast Saccharomyces cerevisiae cell factories; therefore, efficient recombinant protein production techniques are desirable. In this study, to establish an efficient recombinant protein production technique in S. cerevisiae, the secretory production of recombinant pr...

journal_title：Enzyme and microbial technology

authors： Sasaki Y,Mitsui R,Yamada R,Ogino H

更新日期：2019-02-01 00:00:00

abstract：:Phytate is the major storage form of phosphorus in plants. It is present in cereals and raw materials of vegetable origin used in animal and human diets. However, non-ruminant animals have little phytase activity in their guts and, therefore, cannot digest phytate. As a result, almost all dietary phytate is discharged...

journal_title：Enzyme and microbial technology

authors： Xie Z,Fong WP,Tsang PW

更新日期：2020-06-01 00:00:00

abstract：:Horseradish peroxidase (HRP) was successfully purified from horseradish roots by a two-stage reverse-micellar extraction from the dialyzed aqueous extract. The anionic surfactant AOT dissolved in isooctane was used to produce the reverse-micellar phases. The narrow pH range at which HRP solubilization occurred was exp...

journal_title：Enzyme and microbial technology

authors： Regalado C,Asenjo JA,Pyle DL

更新日期：1996-04-01 00:00:00

abstract：:The heat and mass transfer have been proved to be the important factors in air pressure pulsation for cellulase production. However, as process of enzyme secretion, the cellulase formation has not been studied in the view of microorganism metabolism and metabolic key enzyme activity under air pressure pulsation condit...

journal_title：Enzyme and microbial technology

authors： Chen H,Shao M,Li H

更新日期：2014-03-05 00:00:00

abstract：:In the paper, a green and efficient biosynthetical technique was reported for preparing cadmium sulfide (CdS) quantum dots, in which Escherichia coli (E. coli) was chosen as a biomatrix. Fluorescence emission spectra and fluorescent microscopic photographs revealed that as-produced CdS quantum dots had an optimum fluo...

journal_title：Enzyme and microbial technology

authors： Yan ZY,Du QQ,Qian J,Wan DY,Wu SM

更新日期：2017-01-01 00:00:00

abstract：:The D-amino acid oxidase cDNA gene (daao) of Trigonopsis variabilis was prepared by reverse transcriptase-polymerase chain reaction (PCR) and cloned into Escherichia coli expression vector, pTrc99A, under the control of tac promoter. Expression of daao gene significantly affected the growth and morphology of E. coli. ...

journal_title：Enzyme and microbial technology

authors： Lin L,Chien HR,Wang W,Hwang T,Fu H,Hsu W

更新日期：2000-10-01 00:00:00

abstract：:Peptide T has a sequence (Ala-Ser-Thr-Thr-Thr-Asn-Tyr-Thr) belonging to HIV envelope that is involved in the interaction with CD(4) receptor of T lymphocytes. Research of protease activities towards this peptide is very relevant for AIDS therapy. Characterization of specificity of subtilisin Carlsberg towards this ver...

journal_title：Enzyme and microbial technology

authors： Nedjar-Arroumea N,Paon M,Koralewski F,Friboulet A,Kouach M,Briand G,Leroy Y,Guillochon D

更新日期：2000-03-01 00:00:00

abstract：:3-Mercaptopyruvate (3MPy), a structural analog of 3-mercaptopropionic acid, is a precursor compound for biosynthesis of polythioesters in bacteria. The cost-effectiveness and sustainability of the whole process could be greatly improved by using the cysteine degradation pathway for an intracellular supply of 3MPy. Tra...

journal_title：Enzyme and microbial technology

authors： Andreeßen C,Gerlt V,Steinbüchel A

更新日期：2017-04-01 00:00:00

abstract：:Phenylalanine ammonia-lyase (PAL) gene plays an important role in the synthesis of flavones, lignin, and other bioactive compounds in living organisms. Inonotus baumii, the only known flavone-producing filamentous fungus, is of great importance in the investigation of flavone metabolic pathways. To study the function ...

journal_title：Enzyme and microbial technology

authors： Lin W,Liu A,Weng C,Li H,Sun S,Song A,Zhu H

更新日期：2018-05-01 00:00:00

abstract：:Catalase was covalently immobilized onto florisil via glutaraldehyde (GA) and glutaraldehyde+6-amino hexanoic acid (6-AHA) (as a spacer arm). Immobilizations of catalase onto modified supports were optimized to improve the efficiency of the overall immobilization procedures. The V(max) values of catalase immobilized v...

journal_title：Enzyme and microbial technology

authors： Alptekin O,Tükel SS,Yildirim D,Alagöz D

更新日期：2011-12-10 00:00:00

abstract：:Ergosterol, like cholesterol, has many beneficial physiological activities, and because it has no adverse clinical problem with cholesterol, it is a substance that can be used as a biomaterial in the food and cosmetics industry. However, ergosterol has low oil solubility and is easily crystallized, which is problemati...

journal_title：Enzyme and microbial technology

authors： Park SH,Kim HK

更新日期：2020-09-01 00:00:00

abstract：:l-asparaginase is a pharmaceutically and industrially important enzyme as it has potential to treat different cancers and inhibit acrylamide formation in fried and baked food products. In the present study, an attempt was made to screen for new and novel l-asparaginase producers using a widely applied phenol red and b...

journal_title：Enzyme and microbial technology

authors： Mihooliya KN,Nandal J,Swami L,Verma H,Chopra L,Sahoo DK

更新日期：2017-12-01 00:00:00

abstract：:Acetobacter pasteurianus oxidizes glycidol with high activity, comparable to the oxidation of ethanol. The organism has a preference for the S-enantiomer, and the kinetic resolution process obeys a simple relationship, indicating an enantiomeric ratio (E) of 19. The compound is converted into glycidic acid, although a...

journal_title：Enzyme and microbial technology

authors： Geerlof A,Jongejan JA,van Dooren TJ,Racemakers-Franken PC,van den Tweel WJ,Duine JA

更新日期：1994-12-01 00:00:00

abstract：:The selected thermotolerant, lactose-utilizing yeast strain Kluyveromyces marxianus NBIMCC 8362 possesses high specific d-amino acid oxidase activity (60Ug(-1)), which was increased nine-fold (545Ug(-1)) by design of the growth medium and conditions for d-amino oxidase induction. Applying an optimized simple and rapid...

journal_title：Enzyme and microbial technology

authors： Kostova DD,Petrova VY,Kujumdzieva AV

更新日期：2008-01-01 00:00:00

abstract：:The yeast Pichia pastoris is a widely used host for recombinant protein expression, and has recently been engineered for whole-cell biocatalysis. The inducible P(AOX) and constitutive P(GAP) promoters are commonly employed. In this study, the S-adenosyl-L-methionine (SAM) biosynthesis and degradation efficiency of two...

journal_title：Enzyme and microbial technology

authors： Hu X,Chu J,Zhang S,Zhuang Y

更新日期：2014-02-05 00:00:00

abstract：:Human interferon alpha 2b (IFN α2b) is a type I interferon exhibiting antiviral, anti-proliferative and immunomodulatory activities. The clinical outcome of the approved recombinant human IFN α2b drugs in the market suffers from short plasma half-life, rapid clearance and other side effects. Human IFN α2b expression i...

journal_title：Enzyme and microbial technology

authors： Katla S,Yoganand KNR,Hingane S,Ranjith Kumar CT,Anand B,Sivaprakasam S

更新日期：2019-09-01 00:00:00

abstract：:The hydrolytic activity of a thermophilic cyclomaltodextrinase (CMD) from Anoxybacillus flavithermus ZNU-NGA and a representative single mutant were investigated against soluble substrates including α-, β- and γ-cyclomaltodestrines (CDs). Based on the occurrence of arginine (Arg) at position 403 in some homologue prot...

journal_title：Enzyme and microbial technology

authors： Mirzaee Z,Jafarian V,Shirdel SA,Khalifeh K

更新日期：2019-12-01 00:00:00

abstract：:Amoxicillin can be produced in an enzymatic suspension-to-suspension reaction in which the substrate(s) and product(s) are mainly present as solid particles, while the reaction takes place in the liquid phase. During these suspension-to-suspension reactions different subprocesses take place, such as dissolution/crysta...

journal_title：Enzyme and microbial technology

authors： Diender MB,Straathof AJ,van der Does T,Zomerdijk M,Heijnen JJ

更新日期：2000-11-01 00:00:00

abstract：:We generated a bifunctional enzyme chimera containing the xylanase and lichenase coupled with SpyTag between them. Meanwhile, we generated another chimera containing SpyCatcher and elastin-like polypeptides (ELPs). As ELPs could bond to the xylanase-lichenase chimera through SpyTag/SpyCatcher spontaneous reaction in m...

journal_title：Enzyme and microbial technology

authors： Lin Y,Jin W,Wang J,Cai Z,Wu S,Zhang G

更新日期：2018-08-01 00:00:00

abstract：:The parameters of biosensors based on the yeast strains Candida maltosa VKM Y-2359, Candida blankii VKM Y-2675, and Debaryomyces hansenii VKM Y-2482 for biochemical oxygen demand (BOD) detection are compared. The catalytic activity of the strains was analyzed in relation to the growth phase. The possibility of using D...

journal_title：Enzyme and microbial technology

authors： Arlyapov V,Kamanin S,Ponamoreva O,Reshetilov A

更新日期：2012-04-05 00:00:00

abstract：:This study aimed to improve the thermostability of a newly cloned tyrosinase from Streptomyces kathirae SC-1. The POPMuSiC algorithm was applied to predict the folding free energy change (ΔDG) of amino acid substitution. Site-directed mutagenesis was used to construct mutants (Q7K, G234P, and Q7K/G234P), and the mutan...

journal_title：Enzyme and microbial technology

authors： Guo J,Rao Z,Yang T,Man Z,Xu M,Zhang X,Yang ST

更新日期：2015-09-01 00:00:00

abstract：:In the previous study, glutathione production was elevated by adding precursor amino acids and adenosine triphosphate (ATP) in high cell density cultivation of Candida utilis. Furthermore, in the present research, glutathione production was further improved by optimizing sodium dedecyl sulfate (SDS) addition coupled w...

journal_title：Enzyme and microbial technology

authors： Liang G,Mo Y,Du G

更新日期：2010-05-05 00:00:00

abstract：:The present study investigated the influence of low-shear modeled microgravity (LSMMG) conditions on Listeria monocytogenes stress response (heat, cold, and acid), membrane fatty acid composition, and virulence potential as well as stress-/virulence-associated gene expression. The results showed that LSMMG-cultivated ...

journal_title：Enzyme and microbial technology

authors： Sheet S,Yesupatham S,Ghosh K,Choi MS,Shim KS,Lee YS

更新日期：2020-02-01 00:00:00

abstract：:Turanose is a natural isomer of sucrose. It is an emerging functional sweetener of the next generation. Turanose is catalytically synthesized from the sucrose biomass by employing amylosucrase enzyme. In this study, a novel gene encoding amylosucrase (Asmet) has been identified from the metagenome of a thermal aquatic...

journal_title：Enzyme and microbial technology

authors： Agarwal N,Narnoliya LK,Singh SP

更新日期：2019-12-01 00:00:00

abstract：:Nine proteins with lignin peroxidase activity were separated from cultures of Phanerochaete chrysosporium INA-12 in glycerol as carbon source and non-nitrogen limited. Four lignin peroxidase isozymes (4, 5, 8, 9) were purified and characterized. Although differences in kinetic parameters could be shown, antibody react...

journal_title：Enzyme and microbial technology

authors： Odier E,Delattre M

更新日期：1990-06-01 00:00:00